Tradicionalni sirevi s područja zapadnog Balkana

Tradicionalni sirevi s područja
Zapadnog Balkana
–
Uspoređivanje mikrobne raznolikosti
Aljoša Trmčič*,
Department of Animal Science, Biotechnical Faculty, University of Ljubljana,
Groblje 3, SI-1230 Domžale, Slovenia
Edita Kasumović,
Faculty of Agriculture and Food Science, University of Sarajevo,
Zmaja od Bosne 8, BH-71000 Sarajevo, Bosnia and Herzegovina
David Cvetanović,
Department of Animal Science, Faculty of Agriculture, University of Novi Sad,
Trg Dositeja Obradovića br. 8, SR-21000 Novi Sad, Serbia
Mirna Mrkonjić Fuka,
Department of Microbiology, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
Jasmina Havranek,
Dairy Science Department, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
* [email protected]
PAŠKI SIR
KRČKI SIR
PIROTSKI SIR
ISTARSKI SIR
SJENIČKI SIR
KRAŠKI SIR
LIVANJSKI SIR
BOVŠKI SIR
TRAVNIČKI SIR
DOLENJSKI SIR
• TEHNOLOŠKA karakterizacija
?
• HEMIJSKA karakterizacija
• MIKROBIOLOŠKA karakterizacija
• TEHNOLOŠKA karakterizacija
?
- LACTOBACILI (Rogosa, 37°C, anaerobno)
- ENTEROKOKI (KAA, 37°C, aerobno)
• HEMIJSKA karakterizacija
- MEZOFILNI LAKTOKOKI (M17, 30°C, aerobno)
- TERMOFILNI LAKTOKOKI (M17, 42°C, aerobno)
• MIKROBIOLOŠKA karakterizacija
• MICROBIOLOGIC characterization
- LAKTOBACILI (Rogosa, 37°C, anaerobno)
- ENTEROKOKI (KAA, 37°C, aerobno)
- MEZOFILNI LAKTOKOKI (M17, 30°C, aerobno)
- TERMOFILNI LAKTOKOKI (M17, 42°C, aerobno)
DNA
Lactobacili
DNA
Enterococci
DNA
Mesophilic
lactococci
DNA
Thermophilic
lactococci
Krk cheese
Slovenija
Livanjski sir
Hrvatska
Travnički sir
PCR
- Dermobacter hominis
- Cellulomonas biazotea
- Sanguibacter keddieii
- Gardnerella vaginalis
- Actinomyces bovis
- Eikenella corrodens
- Erysipelothrix rhusiopathiae/tonsillarum
25%
?
Croatia
Hrvatska
Slovenia
Slovenija
Lactococcus
Streptococcus
Staphylococcus
Lactobacillus
Pediococcus
Lactococcus
Leuconostoc
Corynebacterium
Streptococcus
Not identified
Other
Lactobacillus
Enterococcus
Enterococcus
Bosnia and Herzegovina
Serbia
Srbija
Bosna i Hercegovina
Staphylococcus
Lactococcus
Corynebacterium
Streptococcus
Pediococcus
Leuconostoc
Streptococcus
Other
Lactobacillus
Corynebacterium
Other
Lactobacillus
Enterococcus
Zaključci:
•
Postoje neke karakteristične mikrobiološke osobine koje bi
mogle predstavljati odraz
- specifičnog sastava osnovne sirovine
- tehnološkog postupka
- geografskog područja proizvodnje
•
Večina karakterističnih mikrobioloških osobina je
minornih.
•
Rezultati ove studije ostavljaju dosta prostora za detaljnije
istraživanje tih minornih karakteristika u budučnosti
Hvala na pažnji !
Hvala na pažnji !
Department of Animal Science, Biotechnical Faculty, University of Ljubljana,
Groblje 3, SI-1230 Domžale, Slovenia
Faculty of Agriculture and Food Science, University of Sarajevo,
Zmaja od Bosne 8, BH-71000 Sarajevo, Bosnia and Herzegovina
Department of Animal Science, Faculty of Agriculture, University of Novi Sad,
Trg Dositeja Obradovića br. 8, SR-21000 Novi Sad, Serbia
Department of Microbiology, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
Dairy Science Department, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
* [email protected]
Microbial diversity in Traditional ewe
cheeses from
Western Balkans Region
.
Aljoša Trmčič*,
Department of Animal Science, Biotechnical Faculty, University of Ljubljana,
Groblje 3, SI-1230 Domžale, Slovenia
Edita Kasumović,
Faculty of Agriculture and Food Science, University of Sarajevo,
Zmaja od Bosne 8, BH-71000 Sarajevo, Bosnia and Herzegovina
David Cvetanović,
Department of Animal Science, Faculty of Agriculture, University of Novi Sad,
Trg Dositeja Obradovića br. 8, SR-21000 Novi Sad, Serbia
Mirna Mrkonjić Fuka,
Department of Microbiology, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
Jasmina Havranek,
Dairy Science Department, Faculty of Agriculture, University of Zagreb,
Svetosimunska 25, HR-10000 Zagreb, Croatia
* [email protected]
Enterococcus
Lactobacillus
Streptococcus
Enterococcus faecalis
Lactobacillus plantarum
Streptococcus hyovaginalis
Enterococcus faecium
Lactobacillus coryniformis ssp. torquens
Streptococcus salivarius
Enterococcus haemoperoxidus
Lactobacillus hamsteri
Streptococcus bovis
Enterococcus durans
Lactobacillus bifermentas
Streptococcus vestibularis
Enterococcus psevdoavium
Lactobacillus rhamnosus
Streptococcus mutans
Enterococcus casseliflavus
Lactobacillus alimentarius
Streptococcus arginosus
Enterococcus gilvus
Lactobacillus pentosus
Streptococcus intermedius
Enterococcus hirae
Lactobacillus fructivorans
Streptococcus gallolyticus ssp. gallolyticus
Enterococcus asini
Lactobacillus jensenii
Streptococcus acidominimus
Enterococcus villorum
Lactobacillus brevis
Streptococcus thermophilus
Enterococcus gallinarum
Lactobacillus sake
Lactobacillus hamsteri
Corynobacterium
Pediococcus
Staphylococcus
Corynobacterium xerosis
Pediococcus pentosaceus
Staphylococcus hominis ssp. hominis
Corynobacterium minutissimum
Pediococcus acidilactici
Staphylococcus capitis ssp. ureolyticus
Corynobacterium freneyi
Pediococcus parvulus
Staphylococcus haemolyticus
Carnobacterium gallinarum
Corynebacterium lipophiloflavum
Corynebacterium mobile
Corynebacterium inhibens
Task 1: Standardisation of technological process conditions and
physical characterisitics of the cheeses
Task 2: Characterisation of physico-chemical and sensory
properties of the cheeses
Task 3: Diversity and dynamics of dominant bacterial population in
cheese samples
Task 4: Cheese fingerprinting
Task 5: The evolution of cpecific characterisitics of the cheeses
during the ripening
Task 6: Publishing of regional atals of the traditional ewe’s
cheeses in westeren blakans and Slovenia.