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D3.3 Evidence for the human health benefits of fresh fruit

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D3.3
Evidence for the human health benefits of fresh fruit in validated human
digestion, bioavailability and disease model systems (P1, P3, P4)
Results of P1 GROUP

Study I:
Protective effect of Sveva strawberry polyphenol-rich
extract against oxidative stress inducted by AAPH on human dermal
fibroblasts.

Study II: Effects of two different strawberry cultivars (Adria and
Sveva) against doxorubicin (DOX)-induced toxicity in rats.

Study III: Effect of 500 g/day of Alba strawberries supplementation
for 1 month on 23 healthy volunteers.
STUDY I
Anthocyanin-rich strawberry extract protects against oxidative stress damage and improves mitochondrial
functionality in human dermal fibroblasts exposed to oxidant agent.
Francesca Giampieri, José M. Alvarez-Suarez, Luca Mazzoni , Tamara Y. ForbesHernandez, Massimiliano Gasparrini, Ana M. Gonzàlez-Paramàs, Celestino Santos-Buelga,
Josè L. Quiles, Bruno Mezzetti, and Maurizio Battino
Submitted
mAU
2000
mAU
2000
1750
1750
HPLC-DAD-MS
mAU
HPLC-DAD-MS
2000
1500
1000
1750
1000
750
1500
750
1500
HPLC-DAD-MS
analysis
analysis
HPLC-DAD-MS
analysis
1250
500
analysis
250
1250
• content
High content
High
of of
phenolic
compounds
phenolic
compounds
• Relevant
Relevant
total total
• High content of
antioxidant
capacity
antioxidant
capacity
phenolic compounds
• Relevant total
antioxidant capacity
•
1250
•
500
1000
250
750
0
10
500
20
0
30
10
40
20
50
30
60
40
min
50
60
min
250
Incubation
for 1024 20
0
40
50
60
min
for 2430AAPH-induced
stress
hours withIncubation
strawberry
hours with strawberry AAPH-induced stress
extract
extract for 24
Incubation
AAPH-induced stress
hours with strawberry
extract
Incubation for 24 h with
strawberry extract
Cell viability
Cell viability
Cell viability
*
Cell viability
**
*
G/R
**
*
***
*
**
***
#
+
+
#
###
+
+
###
+
###
#
8
6
6
4**
0
+++
###
10
4
** **
G/R
***++ ##
###
8
6
2
+++
###
Lipid peroxidation
10
8
*
Lipid peroxidation
Lipid
peroxidation
10
G/R
*
Lipid peroxidation
AAPH induced stress
4
2
**
2
****
00 0.5 11
2
0
++ ##
+++
++ ##
0
+
2.5**
3
00 0.5 11
00 0.5 11
2
2
2.5 3
Mitochondrial
performance
Mitochondrial performance
mM AAPH
4
7
55
9 10 10
8
6
0.5 mg/ml
5
5mM AAPH
6
4
Mitochondrial performance
**
55 ** 6
4
2.5ctrl
3
**
**
**
**
** **
**
**
**
7
7
8
8
9 10 10
9 10 10
mM AAPH
ctrl
ctrl
0.5 mg/ml
0.5 mg/ml
Mitochondrial performance
Protective effects of Sveva against oxidative stress induced by AAPH
STUDY II
Doxorubicin-Induced Oxidative Stress in Rats Is Efficiently Counteracted by Dietary Anthocyanin Differently
Enriched Strawberry (Fragaria x ananassa Duch.)
Effect of Adria and Sveva against DOX-induced toxicity
STUDY III
One-month strawberry-rich anthocyanin supplementation ameliorates cardiovascular risk, oxidative stress
markers and platelet activation in humans
mAU
2000
•
1750
1500
1250
HPLC-DAD-MS
analysis
•
1000
750
Pelargonidin-3glucoside
500
250
0
10
20
30
40
50
•
High content of pelargonidin3-glucoside
Relevant total antioxidant
capacity
High content of Vitamin C
60 min
Biomarkers of oxidative
stress
FRAP, ORAC, Vit C
=
A) Resting platelet
Uric Acid
MDA, 8-OHdG,
Isoprostane
AFTER STRAWBERRY INTAKE
Significant reduction of spontaneous and oxidative hemolysis compared
to the baseline point, which remained stable after the washout period.
B) Central clustering platelets
Lipid profile
TC, LDL-C, TG
C) Degranulated
platelets
Alba supplementation ameliorates cardiovascular risk
Many thanks to:
Prof. Josè L.Quiles
Dr. Sara Tulipani
Prof. Maurizio Battino
Prof. Bruno Mezzetti
Dr. Franco Capocasa
Dr. Jacopo Diamanti
Dr. Stefano Bompadre
Dr. Josè M. Alvarez Suarez
Dr. Francesca Giampieri
Dr. Massimiliano Gasparrini
Dr. Tamara Y. Forbes Hernandez
Prof .Celestino Santos
Buelga
Dr. Ana Paramas
Prof .Dragana Dekanski
Dr. Slavica Ristic
Dr. Nevena V. Radanjic
Dr. Natasa D. Petronijevic
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