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Τηλ: +(357) 22765001
Φαξ: +(357) 22765007
e-mail: [email protected]
Χημικό - Μικροβιολογικό εργαστήριο τροφίμων, νερού και περιβάλλοντος
ΕΡΓΑΣΤΗΡΙΑΚΗ ΕΚΘΕΣΗ/LΑΒΟRAΤΟRγ
ΑΡ. ΕΡΓ. ΕΚΘΕΣIΠ;fREPΟRΤ
Νο.
ΠPOΣj ΑΤΤ. ΤΟ:
ΔΙΕΥΘΥΝΣΗΠΕΛΑΤΗjCLΙΕΝΤ
REPORT
12697
MILITEXBv
ΟΝΟΜΑ ΔΕΙΓΜΑΤΟΣ/ SAMP. ΝΑΜΕ:
ΠΕΡΙΓΡΑΦΗ ΔEIΓMATOΣj SAMPLE DESC. :
ADDRESS:
Amperestraat 3a, 1976 Ijmuiden, Netherlands
AlmondMed Wash 218 - Hand and Skin Washing lotion
Hand and Skin 1ι\fashing lotion
ΆΛΛΕΣ ΛEΠTOMEPEIEΣj OTHER DETAlLS:
In νίνο estinlation of bacteήaΙ reduction after hand washing witll testing
product
ZHTOYMENO/OB~C~
Estimation οΕbacteria110ad οτι artίficially contaminated human l1and afte
hand waslling
ΤΟΠΟΣ Δ/ΛΗΨΙΑΣ/ SAMPLLING ΡΟΙΝΤ:
ΗΜ.. Δ/ΛΗΨΙΑΣ/ SAMPLING DATE:
ΔEIΓMATOΛIΠlTIΠ;f
SAMPLER:
ΚΩΔΙΚΟΣ ΔΕΙΓΜΑΤΟΣ/
SAMPLE CODE:
ΣΥΝΤΟΜΗ ΠΕΡΙΓΡΑΦΗ ΜΕΘΟΔΟΥ/
METHODOLOGY BRIEF DISCRIPllON
ΜΈΘΟΔΟΣ ΕΞΕΤΑΣΗΣ/
ANALYSIS METHOD
ΠΑΡΑΜΕΤΡΟ11
PARAMETER
TPC
--
Rereived at tlle Iab's prenιises - send via courier
Ι
Ι
27/04/2012
E.Papaioannou
ΜΔ-
63
QPAΔIΛHΨIAΣ/SAMP.
ΠΜΕ:
14:30
Ν. Neophytou
ANAΛYTIΠ;f ANALYST:
-01
Artίficial human hand contamination via contact with chicken carcass->
Contact period: 45 seconds-> Air dry-> Hand swabing from whoIe hand
surface including naiIs. ->Hand washing with 1.5 πι! product for 30 seconds
contact time. Hand swabing
ΑΠΟΤΕΛΕΣΜΑΤAL
RESULTS*
ΦΥΣΙΟΛΟΓΙΚΟ
ΟΡΙΟ/ NORMAL
RANGE*
;Μ9ΝΑΔΑ
METPHΣHΣj
MEASUR. UNΠS
150 18593:2004
4.2χ 102
CFU
-
Enterobacteriaceae
NILM-M-21
1.5Χ 102
CFU
-
5taphylococci
NILM-M-20
10
CFU
-
* VALUES ΝΟΤΕD ABOVE 15ΤΗΕ ΑVERAGE OF Α FIVE ΤΙΜΕ REPLICATE PROCEDURE
/ΠΑΡΑΤΗΡΗΣΕΙΣ/
REMARΚS,
T1ιis test report sl1all τιοι be reproduced except in full, ,vitl1out ,vritten approval of the Iaboratory.n1e test report by itself in τιο way constitutes or
implies product approval by tlle body granting accreditation or any otl1er body. The test results relate σηΙΥ to tl1eitem tested. The uncertainty of eadl
equipment or test is avaiJable ση request
END ΟΡ LAB REPORT
__ .~----_-
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·l\ffiΘL.ΑΒ LTD
=-----=-=
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Neophytos Neophytou
Lab Director
Βασιλέως Παύλου 17, Παρισσινότ; 2412 Έγκωμη, Λευκωσία
Http://i-\,,\,,.\'.neolab.com.C)'
NEOLABLTD
Τηλ: +(357) 22765001
Φαξ: +(357) 22765007
e-mail: [email protected]
Χημικό - ΜικροβιολογιΚό εργαστήριο τροφίμων, νερού και περιβάλλοντο;
ΕΡΓΑΣΤΗΡΙΑΚΗ ΕΚΘΕΣΗ/LΑΒΟRAΤΟRΥ
ΑΡ. ΕΡΓ. EΚeEΣm;tREPORT
Νο.
REPORT
12696
MILΠEXBν
ΠPO~AΠ.TO:
ΔΙΕΎΘΥΝΣΗ ΠΕΛΑΤΗ/ CLIENT ADDRESS:
Amperestraat3a, 1976 Ijmuiden, NetherIaηds
AImondMed Wash - Hand and Skin Washing Iotion
ΟΝΟΜΑ ΔEIΓMATOΣj SAMP. ΝΑΜΕ:
ΠΕΡΙΓΡΑΦΗ ΔEIΓMATOΣj SAMPLE DESC.:
Ηετιά and Skin loVashingIotion
ΆΛΛΕΣ ΛEΠTOMEPEIΈΣj OTHER DETAILS:
In vivo quantitation of bacteήal reduction after hand washing with testing
product
ΖΗΤΟΎΜΕΝΟ /OBJECTIVE
Estirnation of bacteήaΙl0ad
before hand washing
ΤΟΠΟΣ Δ/ΛΗΨΙΑΣ!
SAMPLLING ΡΟΙΝΤ:
οτι Ιιιιαιετι hand after artificial contarnination and
Rereived at the Iab's prernises - send via courier
ΗΜ. Δ/ΛΗΨΙΑΣ/ SAMPLING ΌΑΤΕ:
ΔΕΙΓΜΑΤΟΑΗΠΤΗΣ/ SAMPLER:
Κ9....ΔΙΚΟΣΔΕΙΓΜΑΤΟΣ / SAMPLE CODE:
27/04/2012
E.Papaioannou
11.1Δ-63 -01
ΣΥΝΤΟΜΗ ΠΕΡΙΓΡΑΦΗ ΜΕΘΟΔΟΥ/
METHODOLOGY BRIEF DISCRIPTION
ArtificiaI hurnan hand contarnination via contact with chicken carcass->
Contact period: 45 seconds-> Air dry-> Hand swabing ποηι whole hand
surface incIuding nails. ->Hand washing with 1.5 rnI product for 30 seconds
contact tilne. Hand swabing
/
/
ΩPAΔIΛHΨIAΣ/SAMP.
ΤΙΜΕ:
14:30
ΑΝΑΛΥΤΗΣ/ ANALYST:
Ν. Neophytou
-METPHΣIll1
MEASUR. UNΠB
5.6 Χ 105
CFU/ hand surface
-
NILM-M-21
2.1 Χ 104
CFU / l1and surface
-
NILM-M-20
1.7
CFU/ hand surface
-
ΜΕΘΟΔΟΣ ΕΞΕΤΑΣΗΣΙ
ANALYSIS METHOD
ΑΠΟΤΕΑΕΣΜΑΤΑ/
RESULTS:
TPC (Tota1 Plate
Count)
ISO 18593:2004
Enterobacteriaceae
Staphylococci
ΝΟΤΕΟ ABOVE JSΤΗΕ Α VERAGE OF Α F!VE ΤΙΜΕ REPLICATE
ΙΠΑΡ ATHPHΣEIΣj
__ΜΟΝΑΔΑ
ΦΥΣΙΟΑΟΠΚΟ
ΟΡΙΟ/ NORMAL
RANGE*
ΠΑΡ ΑΜΕΤΡΟ4'
PARAMETER
·VALUES
--
χ
103
PROCEDURE
REMARΚS,
ThΊStest report Sllallnot be reproduced except in full, \\':ίthοut1ffitten approval οί the laboratory.The test report by itself in τιο \"ay constίtutes or
ϊmplies product approval by the body granling accreditation οχany otller body. The test results re1ateonly ΙΟ the item testecLThe uncertainty of eacll
equipment or test Ίs available οτιrequest
END OF LAB REPORT
NEq~
~
.~~~
Neophytos Neophytou
. Lab Director
Βασιλέως Παύλου Ιϊ, Παρισσινόξ, 2412 'Εγκωμη, Λευκωσία
Http://;'\'1',,,-\,.neolab.com.c:·
ο
Ne
ΝΕΟΙΑΒ
6'
LTD
Τηλ: +(357) 22765001
Φαξ: +(357) 22765007
e-mail: [email protected]
HAND WASHING ΤΟΤΑΙ ΡΙΑΤΕ COUNT LOG 10 REDUCTlON
ιοο.,
CFU/hand
BEFORE WASHING
ΤΟΤΑΙ ΡΙΑΤΕ COUNT L0910
560000
5.75
420
2.62
AFTER WASHING
BACTERIAL REDUCTION LOG10
3.12
BACTERIAL REDUCTION %
54.36
(----
HAND WASHING ΤΟΤΑΙ ΡΙΑΤΕ COUNT LOG10
REDUCTION
7.00
6.00
5.00
.-t
4.00
Ο
....ι
3.00
c
(!)
~1'~
2.00
1.00
~ ~~~~.~-~--
0.00
,~~
BEFOREWASHING
AFTER WASHING
ΤΟΤΑL ΡιΑΤΕ COUNT Log10
HAND WASHING Enterobacteriaceae
BEFORE WASHING
L0910
AFTER WASHING
\
ιοο.,
CFU/hand
Enterobacteriaceae
ι
LOG 10 REDUCTlON
21000
4.32
150
2.18
BACTERIAL REDUCTION LOG10
2.15
BACTERIAL REDUCTlON %
49.65
HAND WASHING Enterobacteriaceae
LOG10
REDUCTION
4.00
3.00
----------...--
2.00 t=ΞΞΞΞΞΞΞ
1.00 -1------,-0.00
:Ξ-:ΞΞ:\ΞΞΞΞΞΞΞΞ':
'-~~
_~ΞΞΞΞΞΞ
BEFOREWASHING
AFTER WASHING
Enterobacteriaceae
Βασιλέως Παύλου 17, Παρισmvός, 2412 Έγκωμη, Λευκωσία
Log10
'-
Http://-www.neolab.com.cy
JT!
Ne~!ΩJ
NEOLABLTD
ο
Τηλ: +(357) 22765001
Φαξ: +(357) 22765007
e-mail: [email protected]
Χημικό - Μικροβιολογικό εργαστήριο τροφίμων, νερού και περιβάλλοντος
ΗΑΝΟ WASHING STAPHYlOCOCCΙ lOG 10 REDUCTlON
SΤ APHYLOCOCCI
c
ΙΟ910
HAND WASHING STAPHYLOCOCCI LOGI0
REDUCTION
BEFORE WASHING
AFTER WASHING
SΤAPHYLOCOCCI
ΗΑΝΟ WASHING ννΙΤΗουτ
Logl0
SOAP- MECHANICAl ACTlON lOG 10 REDUCTlON
TPC
C~
ιοο.,
BEFORE WASHING
5.06
νν/οuτ
AFTER WASHING
WIPING - PLAIN WAΤER
4.59
AFTER WIPING
2.75
LOGI0 REDUCTION OF WATER AND WIPING
MECHANICAL ACTION
BEFORE WASHING
AFTER WASHING
WIPING
Βασιλέως Παύλου 17, Παριοοινόι;
ννιοuτ
AFΤER WIPING
- ΡLΑΙΝ WAΤER
2412 Έγκωμη, Λευκωσία
Http:j jwww.neolab.com.cy
NEOLABLTD
Ne
Τηλ: +(357) 22765001
Φαξ: +(357) 22765007
e-mail: [email protected]
Χημικό - Μικροβιολογικό εργαστήριο τροφίμων, νερού και περιβάλλοντος
RESULTS:
The soap product tested (AlmondMed)
Gram negative Enterobacteriaceae
showed a total plate count reduction of 3.12 Log10' The
were reduced 2.15 Log10 and the Gram positive staphylococci
were reduced 2.23Log1o.
The test also provided data as to what is the effect of the hand rubbing procedure during water
washing σπ the bacterialload
present οπ hand skin as well as to what is the effect of the hand
wiping procedure after hand wash. Taking into consideration
can be noted that plain water without
bacterialload
the effect of the mechanical action it
the addition of soap could not reduce significantly the
thus the bacterial reduction observed is mainly due to the use of the soap tested. In
addition a further ca. 50% reduction could be achieved by a following
hand wiping procedure.
CONCLUSION
Due to the lack of internationally
the efficacy of πσπ bactericidal
approved standards σπ specifying the procedure steps of assessing
hand soaps there is πσ threshold value which should be achieved ίπ
order to evaluate the product as effective οτ not. However the obtained values can be assessed by
the laboratory as satisfactory according to the product description.
The product has been evaluated
ίπ vίvσ under conditions which human hands have been contaminated
as well as microbiologicalload.
with animal proteins and fat
The organic matter present οπ hands (animal proteins and fat)
played a significant role ίπ deactivating
active ingredients of the product tested thus it provided
harsh conditions for assessing its efficacy.
LEGAL ΝΟΤΕ
(
--
The current assessment relates only to the samples submitted to the labσratσry and ίπ πσ way
constitutes
preapproved
product οτ batch approval. The methodology
~
and the scale of the assessment has been
--
by the customer.
fn-
1
CJ
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[-------Βασιλέως Παύλου 17, Παρισmνός, 2412 Έγκωμη, Λευκωσία
L
Http://www.neolab.com.c).