X INCONTRO DEI DOTTORANDI IN ECOLOGIA E SCIENZE DEI SISTEMI ACQUATICI Palermo, 07 – 10 Aprile 2014 Orto Botanico dell’Università di Palermo PROGRAM AND ABSTRACT BOOK Prefazione Cari partecipanti al “X Incontro dei Dottorandi in Ecologia e Scienze dei Sistemi Acquatici”, Fare ricerca in Italia oggi è una sfida titanica. Esiguità di fondi ed eccesso di burocrazia appesantiscono il lavoro del ricercatore distraendolo dal suo reale compito: produrre idee. Questo scenario, aggravatosi nel corso degli ultimi anni, interferisce con la qualità e la quantità della produzione di idee che largamente coincide con la produzione scientifica. Infatti, così come un paese oberato dal carico fiscale stenta a far decollare la sua economia, un ricercatore sepolto in mezzo alle scartoffie non avrà né il tempo né la serenità per fare ricerca. Considerando inoltre che il sistema accademico italiano è largamente malato di nepotismi e, molto spesso, autoreferenziale ne risulta un panorama generale desolante. Peraltro le cure escogitate dal politico di turno per sanare le discrasie del sistema ricerca hanno spesso prodotto più danni che reali vantaggi. Per non parlare dei trattamenti economici riservati agli insegnanti e ai ricercatori in Italia. Coloro che si dedicano alla ricerca devono essere quindi spinti da una passione sconfinata per continuare a svolgere il loro lavoro in modo produttivo/creativo. In coloro che, all’inizio della carriera, si accingono a fare della ricerca la loro scelta di vita questa passione deve essere travolgente (a tal punto dall’essere scambiata talvolta per mancanza di sanità mentale). L’Associazione Italiana di Oceanologia e Limnologia e la Società Italiana di Ecologia nel lanciare l’iniziativa dell’ incontro annuale dei dottorandi in ecologia e scienze dei sistemi acquatici, giunta ormai alla X edizione, hanno riconosciuto la bellezza di questa passione e la necessità di incentivarla e motivarla. La passione e le idee dei dottorandi di oggi rappresentano le potenzialità future di ricerca nel nostro paese. Potenzialità che bisogna coltivare e far crescere, nonostante tutte le difficoltà, e a cui è importante dare la stessa attenzione dovuta alle cose fragili. Come ormai può essere definita tradizione, durante il X incontro dei Dottorandi in Ecologia e in Scienze dei Sistemi acquatici, le idee dei giovani ricercatori saranno presentate, confrontate, discusse ed eventualmente criticate tra pari per produrre una scienza migliore, più solida, indipendente e innovativa. Anche in questa edizione alle presentazioni si affiancano attività tutoriali rivolte all’acquisizione degli strumenti necessari all’esposizione dei risultati ottenuti. Il workshop di apertura sarà quest’anno dedicato all’esplorazione degli strumenti statistici, imprescindibili per l’analisi dei risultati e la validazione delle ipotesi. In particolare, il Dr. Nico Salmaso condurrà i partecipanti alla scoperta di “R”, il potente software di computo statistico e rappresentazione grafica dei risultati liberamente disponibile in Internet. Un ulteriore momento informativo è costituito dal forum su “Ottimizzazione nell’uso dei dati ecologici” volto a fornire ai partecipanti alcune informazioni sui principi di raccolta ed elaborazione dei dati scientifici. Mi auguro che l’incontro possa rappresentare un momento di scambio proficuo ed arricchimento reciproco per tutti voi! Buon lavoro! Il Presidente AIOL Luigi Naselli-Flores Indice PROGRAMMA ................................................................................................................................... 5 PRESENTAZIONI ORALI ................................................................................................................. 8 Biodiversità Stabilità e Funzionamento Ecosistemico ..................................................................... 8 Eco-biologia dei sistemi acquatici ................................................................................................. 15 Ecotossicologia ed Ecologia Microbica ......................................................................................... 22 POSTER ............................................................................................................................................. 29 Biodiversità Stabilità e Funzionamento Ecosistemico ................................................................... 29 Eco-biologia dei sistemi acquatici ................................................................................................. 40 Ecotossicologia ed Ecologia Microbica ......................................................................................... 42 PROGRAMMA X INCONTRO DEI DOTTORANDI IN ECOLOGIA E SCIENZE AMBIENTALI Palermo, 07 – 10 Aprile 2014 Orto Botanico dell’Università di Palermo (Via Lincoln, 2) 07 Aprile, lunedì 08.30-09.30: Registrazione all’Incontro 09.30-10.00: Opening 10.00-18.30: Workshop 19:30: Ice-break 08 Aprile, martedì 09.30-10.45: Presentazioni orali: Ecotossicologia ed Ecologia Microbica 09.30-09.45: Application of innovative methods to evaluate the ecotoxicity of Diethylene glycol Canepa Sara 09.45-10.00: Insights on the toxic effect of Ostreopsis cf. ovata: the role of direct/indirect contact with microalgal cells Giussani Valentina 10.00-10.15: Growth of microalgal biomass on supernatant from biosolids dewatering Marazzi Francesca Alice 10.45-12.45: Tavola Rotonda: “Ottimizzazione nell’uso dei dati ecologici” Relatori: Dott. Bertoni R., Prof. Mazzola A., Prof. Naselli-Flores L., Dott. Salmaso N. Pausa Pranzo 14.45-15.30: Presentazioni orali: Ecotossicologia ed Ecologia Microbica 14.45-15.00: Development of new biosensing techniques to monitor marine pollution Morgana Silvia 15.00-15.15: Evaluation of spatial distribution and accumulation of novel brominated flame retardants, HBCD and PBDES in an Italian subalpine lake using zebra mussel (Dreissena polymorpha) Poma Giulia X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 5 15.15-15.30: Biosurfactant production by bacteria isolated from filter-feeding organisms (Annelida:Polychaeta) Rizzo Carmen 15.30-16.15: Poster corner:Biodiversità Stabilità e Funzionamento Ecosistemico Relatori: Andolina C., Colla S., Conte A., Durante G., Incagnone G., Mazzotta L., Mirasole A., Noè S., Papale M., Rizzo L. 16.15-16.45: Break – Poster display 16.45-17.30: Presentazioni orali: Biodiversità Stabilità e Funzionamento Ecosistemico 16.45-17.00: Study of the macrozoobenthic community within the Grand Harbour, Valletta, Malta D’Alessandro Michela 17.00-17.15: Quantile regression analysis as predictive for lake macroinvertebrate biodiversity Fornaroli Riccardo 17.15-17.30: Effects of chronic bottom trawling disturbance on benthic communities in the Southern Tyrrhenian Sea Mangano Maria Cristina 17.30-18.30: Discussione aperta sui contributi presentati 09 Aprile, mercoledì 09.45-10.30: Presentazioni orali: Biodiversità Stabilità e Funzionamento Ecosistemico 09.45-10.00: Response of macrozoobenthos to synergistic effect of contaminants in the Mar Piccolo of Taranto Nasi Federica 10.00-10.15: Benthic microalgal community composition and trophic status of sediments in the port of Trieste Rogelja Manja 10.15-10.30: A municipality, an ecosystem Visani Ilaria 10.30-11.30: Poster Corner: Ecobiologia dei Sistemi Acquatici & Ecotossicologia Relatori: Bonfardeci A., Capelli C., Cattano C., Di Donato G., Gjoni V., La Marca E.C., Napoli S., Pecoraro C. 11.30-12.00: Break – Poster Display X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 6 12.00-12.45: Presentazioni orali: Ecobiologia dei Sistemi Acquatici 12.00-12.15: Influence of water transparency and lake morphology on the distribution of macrophytes along the depth gradient Azzella Mattia Martin 12.15-12.30: Infection of native crab species and of the invasive Callinectes sapidus by the dynoflagellate Hematodinium spp. in two Mediterranean coastal habitats Carrozzo Leonardo 12.30-12.45: Impacts of dense shelf water cascading in the southern Adriatic Sea: an integrated approach to evaluate small-scale variability on the benthic compartment Conese Ilaria Pausa Pranzo 15.00-16.00: Presentazioni orali: Ecobiologia dei Sistemi Acquatici 15.00-15.15: Relationship between seasonal variation of carbonate coccolithophores in the gulf of Trieste Ingrosso Gianmarco system and 15.15-15.30: MultiBeam EchoSounder (MBES) backscatter signal analysis in marine environment: Applications to study 3D pelagic fish resources Innangi Sara 15.30-15.45: Environmental drivers for the secular evolution of Lake Garda as outlined by paleolimnological reconstructions Milan Manuela 16.00-16.30: Break – Poster Display 16.30-17.30: Discussione aperta sui contributi presentati 17.30-18.30: Premiazioni, Conclusioni e Saluti Finali CENA SOCIALE 10 Aprile, Giovedì 10.30-12.00: Visita guidata all’Orto Botanico X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 7 PRESENTAZIONI ORALI Sessione 1 Biodiversità Stabilità e Funzionamento Ecosistemico X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 8 Study of the macrozoobenthic community within the Grand Harbour, Valletta, Malta D’Alessandro M.1,2, Andaloro F.2, Deidun A.3, Esposito V.1, Giacobbe S.2, Romeo T.1 1 ISPRA, Institute for Environmental Protection and Research, STS Palermo, Laboratory of Milazzo, Via dei Mille, 46 - 98057 Milazzo (ME), Italy. [email protected] 2 ISPRA Institute for Environmental Protection and Research, STS Palermo, c/o Marbela Residence, via Salvatore Puglisi 9,90143 Palermo, Italy 3 University of Malta, Physical Oceanography Unit, Msida MSD 2080, Malta 4 Department of Biological and Environmental Science, University of Messina, Viale Stagno d’Alcontres, 31–98166 S. Agata, Messina, Italy Benthic invertebrates, in view of their rapid and consistent responses to natural or anthropogenic stress, are widely used to assess the status of and anthropogenic impacts on the marine environment. The present study (carried out in the BIODIVALUE Project PO ITALIA-MALTA 2007-2013) reports on the preliminary description of the macrozoobenthic community within the Grand Harbour, of Valletta (Malta, Central Mediterranean), analyzed as a proxy of the degree of sediment pollution. This port is exposed to different levels of anthropogenic pressures and impacts as a result of several activities, such as electrical powerstations, vessel repair and decommissioning, grit-blasting, shipping and maritime traffic. Benthic macrofauna was collected by means of a 0.1 m2 Van Veen grab during June 2013, at six sampling stations from 4.5 to 23 m depth. Univariate and multivariate statistical analyses were applied in order to identify any possible differences in the benthic community structure at the different sampling stations and to investigate any putative correlations between such data and granulometric one; the presence and distribution of plastic debris (marine litter) were also examined. A total of 1385 specimens belonging to 41 families were recorded. Cirratulidae, Corbulidae, Spionidae and Capitellidae were the most abundant families, testifying of a generalized condition of environmental instability, with peaks of disturbance that may be related with human activities. A direct link between benthic assemblages and distribution of plastic debris has not been found. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 9 Quantile regression analysis as predictive for lake macroinvertebrate biodiversity Fornaroli R.1, Cabrini R.1, Zaupa S.2, Bettinetti R.3, Ciampittiello M.2, Boggero A.2 1 DISAT, Università degli Studi di Milano-Bicocca, Piazza della Scienza 1, 20126, Milano, Italy. Tel: 3484940630, [email protected] 2 CNR Institute of Ecosystem Study (ISE), L.go Tonolli 50/52, 28922 Verbania Pallanza, Italy 3 University of Insubria, Teorethical and Applied Science Dept., Via Dunant 3, 21100 Varese, Italy In Italy, the Water Framework Directive introduced major changes to water management, by making water resources more efficient and enforceable, so, this study aims to highlight some of the potential implications of its implementation for lake management. In this respect, the Life+ INHABIT project was crucial in launching the monitoring plan of lake macroinvertebrates, standardized at national level. Quantile regression analysis was focused on nine lakes, located in two Italian regions (Piedmont and Sardinia), sampled through the national standardized protocol. In order to link macroinvertebrate community characteristics to their habitat, we measured twenty-one variables that represent chemical, physical and morphological characteristics of the environment. We evaluated the limiting action of such variables and selected sampling depth and oxygen percent saturation as the two variables that best explained the number of taxa present and the macroinvertebrate community diversity of a site. We provided a set of minimum expected values along the sampling depth gradient. These results should be taken into account in the development of ecological indices that must be able to recognize and separate the effects of anthropogenic pressures from any natural variability. Finally, the selected models are able to predict the potential of community diversity as a function of environmental characteristics. All of this evidence can help water managers in deciding to initially invest resources in those lakes where the biological communities is expected to be impaired and to identify which lakes can be considered pristine or in nearpristine conditions. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 10 Effects of chronic bottom trawling disturbance on benthic communities in the Southern Tyrrhenian Sea Mangano M. C.1,2, Kaiser M. J.2, Porporato E. M. D.1, Spanò N.1 1 DiSBA Dipartimento di Scienze Biologiche ed Ambientali, Università degli Studi di Messina, Viale F. Stagno d'Alcontres 31, 98166, Messina, Italia. Tel.: +39 3489392878, [email protected] 2 School of Ocean Sciences, Bangor University, LL59 5AB, Menai Bridge, Anglesey, U.K. The benthic communities' responses to trawling disturbance were investigated on a fishing ground scale along a gradient of fishing pressure, following a novel approach on the soft bottoms ecosystems off the Southern Tyrrhenian Sea. The Southern Tyrrhenian Sea has been chosen for the central position in the Mediterranean basin. The continental shelf and slope soft bottoms ecosystems were investigated since historically mostly exploited by the trawling fleets operating in the study area. Impacts of otter trawling activity were detected on: 1) mega-epibenthic communities; 2) macro-infaunal communities; 3) population structure and diet of two scavenger species. The analysis on mega-epibenthic communities took advantage of non-target epibenthic species retained from an experimental otter trawl net.The analysis of Vessel Monitoring System data was performed for the first time in the study area, permitting to detect infaunal communities' responses to a gradient of trawling disturbance and long-term Fishery Exclusion Zone. Positive temporal aggregations of the two astropectinid species Astropecten bispinosus and A. irregularis were analysed in exploited areas. Changes in population arrangement and diet composition were interestingly related to fishing activity. The obtained results allow describing the state of health of benthic communities and the magnitude of fishing intensity exerted, additionally providing insight as to how the trawling disturbance effects might be managed in the future, in a context of ecosystem management approach. The research was developed during the PhD period at the Laboratory of Benthic Ecology (DiSBA, University of Messina). Data analyses were performed in collaboration with Fisheries & Conservation Science Group (School of Ocean Sciences, Bangor University). X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 11 Response of macrozoobenthos to synergistic effect of contaminants in the Mar Piccolo of Taranto Nasi F.1,2, Auriemma R.1, Cibic T.1, Del Negro P.1 1 OGS (Istituto Nazionale di Oceanografia e Geofisica Sperimentale), Sezione Oceanografia, v. A. Piccard 54, I-34151, Trieste, Italy. [email protected] 2 Department of Life Sciences, Università degli Studi di Trieste, v. Valerio 28/A, I-34127 Trieste, Italy Since 1960s, the Mar Piccolo has been affected by a wide spectrum of anthropogenic pressures, leading to severe pollution. This environmental contamination likely affects the abundance and composition of benthic communities. In the framework of RITMARE flag project, the resilience of the macrozoobenthic community, affected by chemical contamination, was evaluated. In June 2013, sediments were sampled at four stations to evaluate structural sediment parameters i.e. granulometry, the content of total carbon (TC) and nitrogen (TN), total organic carbon (TOC) and biopolymeric carbon (BPC). Oxygen microprofiles were carried out in the surface sediments. TOC content was generally high ranging from 38.1 mg C g-1 to 42.9 to mg C g-1, while the total macrozoobenthic abundance ranged between 589 ind. m-2 and 1911 ind. m-2. Stations were characterized by the presence of typically marine species or, in contrast, by the presence of species typical of paralic environments. Few alien species, the isopod Paracereis sculpta and the bivalve Arcuatula senhousia, were observed. Almost all encountered taxa are characteristic of areas with a high organic carbon content and low oxygen concentration (e.g. the bivalve Corbula gibba and the polychaete Notomastus latericeus). The composition of macrozoobenthic community at different stations reflects the hydrodynamism of the area and confirms the role of marine waters exchange through the canals of the Mar Grande. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 12 Benthic microalgal community composition and trophic status of sediments in the port of Trieste Rogelja M.1, Cibic T.1, Del Negro P.1 1 OGS (Istituto Nazionale di Oceanografia e Geofisica Sperimentale), Sezione Oceanografia, v. A. Piccard 54, I-34151, Trieste, Italy. Tel.: 0402249740, [email protected] The purpose of this study was to contribute to promote ecosystem-based management and its application in coastal areas, grounded on a better understanding of the ecosystem functionality. In particular, this study aimed to assess the benthic ecosystem trophic status and the response of the microalgal community to multiple and diffuse anthropogenic impacts in a harbour area. Sampling was performed in spring 2013 at 7 sites, characterized by different degrees of impact, inside the Port of Trieste. In each site, the structural characteristics of different types of sediments were defined; measurements of photosyntethically active radiation (PAR), water temperature and salinity were recorded in the water column. The microphytobenthic total abundance and community structure were evaluated and the main energy storage processes (primary production) and dissipation (respiration) were also estimated. Within the microalgal community, taxa resistant to pollutants were identified. At all investigated stations the qualitative composition of the microalgal population was similar and diatoms represented the dominant phylum. We observed 26 genera of diatoms with Nitzschia beeing the most represented. St. 2 and 3 were caracterized by the presence of Nitzschia cfr. commutata, a species more tollerant to pollution, with relative abundances of 22.6% and 14.2%, respectively. Only St. 6 with an extremely high value of net production (64 mgC m-2 h-1) and the barely positive St. 5 (1.53 mgC m-2 h-1) were net autotrophic. At all other sampled stations the benthic system was net heterotrophic. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 13 A municipality, an ecosystem Visani I.1, Aschonitis V.1, Fano E. A1. 1 Department of Life Sciences and Biotechnology , University of Ferrara, Italy. Tel.: 3384872832, [email protected] So far the studies on urban metabolism regard only few cities of the world, mostly large metropolitan areas and cities. With this research we try to go one step forward in this kind of analyses, applying the urban metabolism and Material Flow Analysis theories on small to medium systems. So we think it will be possible to overcome the existing difficulties obtaining universally recognized and comparable data. Moreover small and medium cities are much more widespread than megalopolis so the importance of this target is unquestionable. We tried to elaborate a methodology simple and proper enough to be considered effective but also flexible enough to allow to reflect the peculiarities of the considered area. This approach were applied to the Municipality of Faenza, a medium reality in Northern Italy. Estimating urban metabolism required the formulation of two specific indicators: NAS (Net Addiction to Stock) and DMC (Domestic Material Consumption). As a result of these indicators various conclusions could be drawn, such as the high rate of urbanization of the Municipality in the last years, the total export of waste out of the system and the prevalence of imports over exports, that increase the city dependence from outside territories, both for inputs (energy and materials) and outputs (waste). We also compared these results to Paris and its region (Barles, 2009). In conclusion this is another step in order to better understand urban metabolism. Analyzing medium cities permits to spread the computation and the method applied even for obtaining a better governance. Keywords: Urban metabolism, Sustainable cities, City ecosystem, Material Flow Analysis (MFA), Multidisciplinary approach to urban ecology. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 14 Sessione 2 Eco-biologia dei sistemi acquatici X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 15 Influence of water transparency and lake morphology on the distribution of macrophytes along the depth gradient Azzella M. M.1, Bolpagni R.2, Mjelde M.3, Lombardo P.4 1 Department of Environmental Biology, La Sapienza University of Rome, P.le Aldo Moro 5, 00185, RomeItaly 2 Department of Life Sciences, University of Parma, V.le G.P. Usberti 11/A, Italy 3 Section of Integrated Water Resources Management, Norwegian Institute for Water Research (NIVA), Gaustadalléen 21, N-0349 Oslo, Norway, 4 Department of Civil, Architectural and Environmental Engineering (DICEAA), "M. Giustini" Ecology Lab, University of L'Aquila, Coppito Science Center, I-67100 L'Aquila, Italy Underwater light regime is widely considered the principal determinant of submerged aquatic plant distribution. The majority of previous studies have singled out Secchi disk transparency values (SD) as a key empirical factor to explain the maximum depth of macrophyte colonization (Zc). Few studies have investigated the role of lake morphology in structuring aquatic plants beds. Using two datasets (20 Italian lakes and 173 Norwegian lakes), we explored the relationships among Zc, transparency and morphometric traits to investigate their possible effects on macrophyte depth distribution, also in relation to a possible latitudinal effect. The SD–Zc relationship was best described by a similar polynomial correlation in both Italian and Norwegian lakes, emphasizing the importance of nonlinear analysis on non-transformed data in the description of ecological trends. Our results confirm i) a direct dependence of Zc on SD values and demonstrate ii) a significant role of lake area in the descriptive linear model. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 16 Infection of native crab species and of the invasive Callinectes sapidus by the dynoflagellate Hematodinium spp. in two Mediterranean coastal habitats Carrozzo L.1, Pagliara P.2, Zotti M.2, Mancinelli G.2 1 Department of Biological and Environmental Sciences and Technologies, University of the Salento, S.P. Lecce-Monteroni, 73100 Lecce, Italy, Tel.: +393287965136, [email protected] 2 Department of Biological and Environmental Sciences and Technologies, University of the Salento, S.P. Lecce-Monteroni, 73100 Lecce, Italy The brachyuran assemblage was surveyed monthly in the Torre Colimena basin and Acquatina Lagoon, two coastal habitats located on the Adriatic and Ionian coasts of the Salento peninsula (Southern Italy) using crab traps. In addition, the hemolymph of sampled crab specimens was screened using histological techniques in order to verify the occurrence of dinoflagellates belonging to the genus Hematodinium and the speciesspecificity of the parasite-host interaction. The invasive blue crab Callinectes sapidus was sampled in the Torre Colimena basin during the whole experimental period and no other crab species were ever collected. In contrast, in the Acquatina Lagoon four crab species were sampled, including C. sapidus. Noticeably, the blue crab was collected only in spring and summer months, when the abundance of native crab species declined dramatically. The parasitic dinoflagellate Hematodinium sp., known to determine in C. sapidus the degenerative “bitter crab” syndrome in its native habitats, was ubiquitously detected in the hemolymph of blue crabs from both habitats and in native brachyurans collected in the Acquatina Lagoon. Peak abundances of infected specimens occurred in summer months; in this period, significant inter-site differences in the incidence of the infection were observed. The diverse spectrum of potential ecological effects triggered by the CallinectesHematodinium interaction on native brachyuran species is examined, while the potential controlling role of abiotic conditions characterizing the different habitats is discussed. Keywords: Callinectes sapidus; alien species; Hematodinium sp.; bitter crab syndrome; host-parasite interaction; ecological impact X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 17 Impacts of dense shelf water cascading in the southern Adriatic Sea: an integrated approach to evaluate small-scale variability on the benthic compartment Conese I.1, Langone L.1, Miserocchi S.1, Tesi T.2, Boldrin A.3, Prevedelli D.4 1 CNR–ISMAR – Bologna, 3384021765, [email protected] Stockholm University – Stoccolma 3 4 CNR-ISMAR – Venezia, Università di Modena e ReggioEmilia 2 Southern Adriatic Sea is an area of sinking dense water formed in the northern Adriatic shelf during winter, with strong interannual variability. When dense water reaches continental slope in the southern Adriatic Sea falls into the deep basin (cascading) exporting dissolved and particulate matter to the deepest part (O2, CO2, DOC, POC, etc). During the last decade, the process of cascading of dense water has been studied in the western Adriatic continental margin and particularly in the Bari canyon with moored instrumentation. In February 2012 particular climatic conditions caused a strong cooling of the northern Adriatic Sea with consequent formation of shelf water particularly dense. During this period has been set an experiment to analyze the response of deep marine environment to such an extreme event. The thesis aim is to analyze some aspects of the impact of the dense shelf water cascading in the southern Adriatic Sea. In particular to: - study spatial and temporal variations of fluxes of organic and inorganic particles measured by automatic sediment traps, to evaluate relative importance of lateral advective lateral fluxes (cascading) compared to vertical fluxes of marine particles; - characterize qualitatively and quantitatively the biological component of flux particles, in term of both passive flux and swimmer occurrence, particular evaluating the spatial and temporal mesozooplankton variability; - evaluate the impact of dense shelf water cascading on small-scale variability of benthic habitat in the Bari canyon. First results concerning total mass fluxes and description of mesozooplankton collected by sediment traps are shown. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 18 Relationship between seasonal variation of carbonate system and coccolithophores in the gulf of Trieste Ingrosso G.1,2, Cerino F.2, Fornasaro D.2, Cabrini M.2, Comici C.2, De Vittor C.2, Kralj M.2, Del Negro P.2, Giani M.2 1 University of Trieste, Italy. Tel: +39 040 2249732, [email protected] Istituto Nazionale di Oceanografia e di Geofisica Sperimentale (OGS), Oceanography Section (OCE), Via Auguste Piccard 54, 34151 S.Croce (TS) - Italy 2 The uptake of anthropogenic CO2 emissions by the oceans has caused and will continue to cause a seawater pH decrease. This process, termed as “ocean acidification”, is projected to change drastically the marine carbonate system and represents a threat to marine species, communities, and ecosystems. Coccolithophores are marine calcifying microalgae which are expected to respond to the ongoing changes in seawater CO2 system. However the influence of carbonate chemistry parameters on coccolithophores’ dynamics remains still unclear. The present work investigates the coccolithophore seasonal variation in the Gulf of Trieste during two years of sampling (March 2011-February 2013) in relation to high quality measurements of the carbonate system and other environmental parameters. A strong seasonal variability of the marine CO2 system was found, as a result of different physical and biological processes such as air-sea CO2 gas exchange, riverine inputs, mixing, and net community production. Coccolithophores were more abundant in autumn and winter, whereas during spring-summer periods they were very scarce or undetectable. This seasonal pattern was mainly linked to [HCO3-], which is the preferential form used by coccolithophores for their intracellular calcification. Other related parameters were dissolved inorganic carbon, [CO32-], salinity, and phosphates, whereas the other nutrients did not seem to play a significant role. Our results clearly show that carbonate system could be considered a crucial ecological factor for the determination of coccolithophore abundance and distribution. Nevertheless more investigations are necessary to confirm the robustness of the described relations, also considering the different life stages of these organisms. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 19 MultiBeam EchoSounder (MBES) backscatter signal analysis in marine environment: Applications to study 3D pelagic fish resources Innangi S.1, Bonanno A.2, Tonielli R.1 1 Istituto per l’Ambiente Marino Costiero sede, CNR, Naples, Italy Istituto per l’Ambiente Marino Costiero U.O.S. Capo Granitola, CNR, Mazara, Italy 2 The MultiBeam EchoSounder (MBES) had a remarkable technological development for marine sciences. As a matter of fact, these instruments provide bathymetric, morphological and seafloor composition information. Currently it is possible to study the backscatter of the whole water column, which is useful, for example, to identify fish schools. This PhD research project was aimed to study 3D pelagic fish resources with MBES water column backscatter. This made it possible to characterize the main geometries (i.e. volume, shape, size, etc.) of schools of fish and their spatial distribution. Also in this work were compared data collected with 2D visualization scientific echosounder and 3D ones with MBES, in order to show it is possible to obtain more realistic estimates in the monitored areas. The data was acquired in monitoring of the anchovies (Engraulis encrasicolus; Linneo, 1758) and sardinia (Sardina pilchardus; Walbaum, 1792) fish stocks of the GSA16 (Channel of Sicily), MEDIAS (MEDIterranean Acoustic Surveys), financed by European Data Collection Framework (DCF). X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 20 Environmental drivers for the secular evolution of Lake Garda as outlined by paleolimnological reconstructions Milan M.1,2 1 Sustainable Agro-ecosystems and Bioresources Department, IASMA Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige (TN), Italy. 0461- 650256, [email protected] 2 Department of Ecology and Environmental Science, Umeå University, 901 87 Umeå, Sweden. [email protected] Lake Garda, the largest Italian lake, is divided in two sub-basins: the NW deepest one (350 m) and the SE shallowest one (81 m). A regular monitoring program of lake chemistry and biology has been started in the early 1990s. Before that, only sporadic limnological measurements are available. Lake sediment records provide a complementary source of information to extend the time span of ecological records back into the past, through the reconstruction of secular lake evolution. Radiometrical dating, geochemical (water and LOI content) and biological proxies (algal pigments and diatoms) are being analysed in two short cores (56 cm and 65 cm) retrieved from the deepest point in each basins. The subfossil diatom assemblages analysis show two major changes in both cores. The first one, in mid 1940s, consists of a decrease in benthic taxa. The second change consists of an increase in the relative abundance of planktonic Fragilariaceae around 1960, accompanied by a decrease in the most common centrics (i.e. Cyclotella comensis). The diatom based reconstructions of lake TP concentration in the two lake basins show very stable oligotrophic conditions since the early 1960, followed by a consistent increase peaking in the late 1980s. The limnological data confirm the long-term TP trend. Non-metric multidimensional scaling (NMDS) analyses, coupled with correlation analyses with a set of environmental variables, were performed on sub-fossil diatom assemblages. The analysis outputs indicate the ecological lake evolution as strongly correlated with air temperature, and changes in NAO and EA teleconnection indices. This confirms recent findings (Salmaso and Cerasino, 2012), demonstrating how inter-annual changes in trophic conditions of Lake Garda are largely controlled by climatic fluctuations. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 21 Sessione 3 Ecotossicologia ed Ecologia Microbica X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 22 Application of innovative methods to evaluate the ecotoxicity of Diethylene glycol Canepa S.1, 2, Tornambè A.2, Manfra L.2 1 Università degli Studi di Genova. Tel.: 3477681144, [email protected] ISPRA Istituto Superiore per la Protezione e la Ricerca Ambientale, Via V. Brancati 48, 00144 Roma 2 The present research concern to the application of innovative methods for assessing the ecotoxicity of environmental contaminants. We want to evaluate the long-term toxicity of diethylene glycol (DEG ), an additive largely used during oil and gas exploitation process to prevent hydrate formation and corrosion events. It may be within produced formation water (the main discharge of offshore platforms) and discharged into the sea. Thus, toxicity data are necessary to avoid marine pollution. The ecotoxicological assessment focused on crustaceans, molluscs and fish. The main objectives of the study regards : the selection of a method, among the most recent standardized protocols, which involves the use of marine crustaceans in the Mediterranean Sea; the development of a long-term toxicity test on marine molluscs since the literature offers only guidelines to evaluate the acute and chronic exposure of freshwater species; the assessment of suitable conditions for the application of the protocol reference C14 (CE 440/200) for freshwater species to a native marine species. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 23 Insights on the toxic effect of Ostreopsis cf. ovata: the role of direct/indirect contact with microalgal cells Giussani V.1, Costa E.2, Sbrana F.3, Asnaghi V.1, Vassalli M.3, Faimali F.4, Casabianca S.5, Penna A.5, Ciminiello P.6, Dell’Aversano C.6, Tartaglione L.6, Mazzeo A.6, Garaventa F.2, Lavorano S.7, Chiantore M1 1 DISTAV- University of Genoa. Tel.: +39 010 3538563, [email protected] CNR –Institute of Marine Sciences (ISMAR), Venezia, Italy 3 CNR – The Biophysics Institute (IBF), Genoa, Italy 4 CNR –Institute of Marine Sciences (ISMAR), Genoa, Italy 5 DISB - University of Urbino, Italy 6 University Federico II, Napoli, Italy 7 Acquario di Genova e Costa Edutainment S.p.A, Genova, Italy 2 Ostreopsis cf. ovata is a harmful benthic dinoflagellate, widespread along most of Mediterranean coasts. It produces several toxins and variable amount of mucus that may totally cover substrates, especially during the stationary phase of blooms, in which cells are aggregated. Studies on different aspects of the biology and ecology of Ostreopsis spp. are increasing, yet knowledge on toxicity mechanism are still scarce. In particular, the potential active role of the mucilaginous matrix has not yet been proved, although when mass mortalities have occurred, organisms were reported to be covered by the typical brownish mucilage. In order to better elucidate toxicity dependence of direct/indirect contact with O. cf. ovata cells, we carried out toxicity bioassays exposing Artemia salina nauplii and Aurelia aurita ephyrae to several treatments of O. cf. ovata culture. Our results have reported higher effects when a direct contact between model organisms and intact microalgal cells occurs, triggering further investigation on the discrimination between the role of the cells and its mucous filaments in conveying toxins to the organisms. Results of this second test, combined with chemical analysis, provide evidence, for the first time, of a significant toxic effect (p < 0.01) on A. salina also after exposition to growth medium (devoid of microalgal cells) only when containing O. ovata mucilage. Similar trend has been reported also on A. aurita ephyrae. Moreover, the connection between the thecal plates, pores, trychocysts and the mucilaginous matrix was explored by way of atomic force microscopy (AFM) to investigate cell surface at a sub-nanometer resolution, providing a pioneering description of the inner side of O. cf. ovata thecal plates. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 24 Growth of microalgal biomass on supernatant from biosolids dewatering Marazzi F.1, Fornaroli R.1, Cabrini R.1, Sartori L.1, Canobbio S. 1,Mezzanotte V.1, Ficara E.2 1 Dipartimento di Scienze dell’Ambiente e del Territorio, Università degli Studi di Milano-Bicocca, Piazza della Scienza 1, 20126 Milano, IT 2 Dipartimento ingegneria civile e ambientale, Politecnico di Milano, piazza L. Da Vinci 32, 20133 Milano, IT This study shows the preliminary results for the evaluation of the possible use of a photobioreactor within a wastewater treatment plant (WWTP), growing microalgae on the sludge dewatering supernatant from anaerobic digestate. Microalgae are photosynthetic microorganisms with simple growing requirements that can produce lipids, proteins and carbohydrates in large amounts over short periods of time. At the moment microalgae biomass shows great potential as raw material for producing biofuel, and those organism could bring benefit to WWTPs, which at the moment have some critical point, due to low efficiency. This study has two main objectives: removing nutrients (nitrogen and phosphorus) and produce biomass, which can be used as substrate for biogas production by anaerobic digestion. Preliminary tests are in agreement with literature data. In those experiments microalgal inocula (Chlorella Sp and Scendesmus obliquus) are cultivated in semi-continuous reactor; CO2 is artificially supplied and a mixture of treated effluent and sludge dewatering supernatant is used as feed. Light is provided by fluorescent lamps; sludge dewatering supernatants can be well utilized by microalgae as a growth medium and nitrogen removal efficiency is 77%-82%. Specific tests show that the algal biomass has a biomethane potential similar to that of excess sludge. An other option to reduce the production costs and the greenhouse gas emission is to use the off-gas from the WWTP turbine as source of CO2 for the microalgae. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 25 Development of new biosensing techniques to monitor marine pollution Morgana S.1,2, Pronzato R.2, Faimali M.1 1 CNR––Istituto di Scienze Marine ISMAR Sezione Tecnologie Marine, Via de Marini, 6, 16149 Genova DIPARTIMENTO DI SCIENZE DELLA TERRA, DELL’AMBIENTE E DELLA VITA, Università di Genova, Corso Europa 26, 16132 Genova, Italia. [email protected] 2 In the last few years it has become increasingly important the contribution of ecotoxicological assays to the environmental monitoring, as integration of chemical analyses; which are a highly effective analytical tools, capable of assessing the presence of the whole substances and their derivates. However these analyses do not provide data about the effect of chemicals on biota. In fact, chemical contaminants rarely affect organisms as single substances, but instead cause adverse effects as diverse mixture. Therefore chemical analysis need to be integrated with biological methods that assess the toxicity of the contaminant biologically available. For this reason, in the assessment of water quality, an integrated approach is recommended and new ecotoxicological analytical tools are required. The aim of the present study is the optimization of innovative methodologies of ecotoxicological investigation of the marine environment in situ and ex situ, based on the use of biosensing techniques recently developed by ISMAR-CNR Genoa. In a first approach we will investigate the possibility to use an electrochemical biosensor (ALVIM BETA Biosensore Elettrochimico per la Tossicità delle Acque ) for the transduction of natural biofilm to detect in real time environmental quality alteration, in a simple, quick and cheap way (Pavanello et al., 2011). In addition we will optimize and improve a behavioral test (Swimming Speed Alteration Test), using new different model organisms and reducing time of exposure, through SWIMMING BEHAVIOUR RECORDER (SBR system; Faimali et al., 2006), which can be used in laboratory ecotoxicological testing to evaluate the toxicity associated to toxic compounds and environmental samples. Bibliografia faimali m., garaventa f., piazza v., greco g., corra’ c., magillo f., pittore m., giacco e., gallus l., falugi c., tagliafierro g. (2006). Swimming speed alteration of larvae of Balanus amphitrite (Darwin) as a behavioural end-point toxicological bioassays. Mar. Biol. 149 (1): 87-96. pavanello g., faimali m., pittore m., mollica a., mollica a., mollica a. (2011). Exploiting a new electrochemical sensor for biofilm monitoring and water treatment optimization. Water Research 45, 1651–1658. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 26 Evaluation of spatial distribution and accumulation of novel brominated flame retardants, HBCD and PBDES in an Italian sub-alpine lake using zebra mussel (Dreissena polymorpha) Poma G.1, Binelli A.2, Volta P.3, Roscioli C.1, Guzzella L.1 1 CNR-IRSA, Water Research Institute, Via del Mulino 19, 20861 Brugherio, MB, Italy Tel: +39 039 216941; fax: +39 039 2004692, [email protected] 2 Department of Biology, University of Milan, Milan, Italy 3 CNR- ISE, Institute of Ecosystem Study, Largo Tonolli 50, Verbania VB, Italy Because of the reduction in the use of polybrominated diphenyl ethers (PBDEs) and hexabromocyclododecane (HBCD), novel brominated flame retardants (NBFRs), including 1,2-bis(2,4,6-tribromophenoxy) ethane (BTBPE), decabromodiphenyl ethane (DBDPE), hexabromobenzene (HBB), and pentabromoethylbenzene (PBEB), started to be marketed as alternatives to the banned formulations. In this study, the spatial distribution and accumulation of NBFRs, PBDEs, and HBCD in the biota have been investigated in the littoral compartment of Lake Maggiore (Northern Italy), using zebra mussel and roach as bioindicators. To our knowledge, this is the first study reporting the contamination of NBFRs in the freshwater invertebrate zebra mussel. Contamination of zebra mussel due to PBEB, HBB and BTBPE was low, ranging from 0.9 to 2.9 ng/g l.w., from 1.1 to 2.9 ng/g l.w., and from 3.5 to 9.5 ng/g l.w. respectively. PBEB and BTBPE in roach were always <LOD, while the contamination of HBB ranged from <LOD to 1.74 ng/g l.w., indicating a weak contamination. DBDPE was <LOD in all the considered biological samples. Finally, HBCD was detected in all organic tissues with mean concentrations up to 74.4 ng/g l.w. PBDE results, supported by PCA elaboration, suggested a possible contamination due to the congeners composing the Penta- and Deca-BDE technical formulations, which are present in the Lake Maggiore basin. The biomagnification factor (BMF) values showed that tetra- and penta-BDE biomagnified, while octa-, nona-, and deca-BDE were still bioavailable and detectable in the fish muscles, but they do not biomagnified. Considering the other BFRs, only HBCD showed a moderate biomagnification potential. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 27 Biosurfactant production by bacteria isolated from filter-feeding organisms (Annelida:Polychaeta) Rizzo C.1, Graziano M. 1, Michaud L. 1, De Domenico E. 1, Lo Giudice A. 1 1 Department of Animal Biology and Marine Ecology, University of Messina, Viale Ferdinando Stagno d’Alcontres, 31 – 98166 Messina, Italia. [email protected] Three biosurfactant-producing isolates [i.e. Joostella sp. A8 (J), Alcanivorax sp. A53 (A) and Pseudomonas sp. A6 (P), which were previously isolated from enrichment cultures using polychaetes homogenates)] were inoculated in a mineral medium added with diesel oil for 21 days. Experiments were carried out in both microcosm of individual isolates and consortia (by inoculating J with P or A; consortia J-P and J-A, respectively). Bacterial abundance (by fluorescent in situ hybridization, FISH), biodegradation efficiency (by gaschromatographic analysis) and biosurfactant production in microcosms and co-cultures were monitored. Microcosm assays revealed a decreasing growth rate of the isolates in the order P>A>J. Joostella sp. A8 resulted the most promising isolate as it was able to produce the highest stable emulsion (E24) percentage and hydrofobicity rate (78.33 and 62.67%, respectively,), in addition to a surface tension reduction of 33.43 mN/m. In both consortia isolates achieved similar abundance values. Stable emulsions and hydrofobicity rates had a fluctuant pattern, with the best efficiency that was showed by the J-P consortia. Gas-chromatographic analysis revealed a biodegradation efficiency of 52.75%, 38.22%, 26.83% from A, P and J, respectively, when individually inoculated. Such values increased in consortium experiment achieving percentages of 99.39 e 99.17% in JA and J-P, respectively. Results were useful to compare the efficiencies of well-known biosurfactant producers, such as Pseudomonas spp. and Alcanivorax spp., with respect to a still unknown biosurfactant producer, i.e. Joostella spp. Moreover, the investigations carried out on consortia put on evidence that biodegradation efficiency was improved by the contemporary action of different bacterial species. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 28 POSTER Sessione 1 Biodiversità Stabilità e Funzionamento Ecosistemico X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 29 Using food web metrics as descriptors to evaluate variation of trophic organization at spatial and temporal scales Andolina C.1,2, Franzoi, P.1, Vizzini S.2 1 Department of Environmental Sciences, Informatics and Statistics, Ca' Foscari University of Venice, Italy. +39 3883659542, [email protected] 2 Department of Earth and Marine Sciences, University of Palermo, Italy To deepen the understanding of potential consequences of the environmental change on ecosystems is one of the most important challenges ecologists are currently facing. Interactions between species represent the basic structure on which ecological communities are based: among these, trophic interactions are useful tools to investigate the degree of complexity of the systems. The aim of this project is to characterize the trophic structure of coastal marine ecosystems, with the purpose to detect its variations at spatial and temporal scale and to assess its potential role as indicator of environmental change. In order to evaluate the contribution provided by different habitats to the trophic organization of fish populations and communities and to the width of their ecological niche, we will test food web metrics at different spatial and temporal levels, by examining 1. the trophic niche of an estuarine resident fish, Aphanius fasciatus, in different habitats (salt marsh, unvegetated mudflat, seagrass beds and salt evaporation pond) at inter and intra system level (in Stagnone di Marsala and Venice lagoon); 2. the diet of a migratory species, Sparus aurata, whose different life stages occur in different habitats within and outside the Venice lagoon; 3. the trophic structure of a lagoon area under seagrass restoration process. We expect from this study to obtain important information on lagoon trophic functioning and on selection of appropriate food web descriptors as indicators of environmental conditions also in the light of the Marine Strategy Framework Directive. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 30 The role of offshore mussel culture farms in relation to the structure and function of the adriatic coastal ecosystem Colla S. 1 1 CEMAS, Center for estuarine and coastal marine sciences, dipartimento scienze ambientali, informatica e statistica, Università Ca’ Foscari, castello 2737/b – 30120 Venezia – Italia. [email protected] At present time, offshore mussel culture farms (MCFs) can be considered as one of the major driver affecting both the structure and functioning of the coastal area ecosystem in the Northern Adriatic Sea. The research project will focus on the MCFs, which can be considered as a sort of ‘mesocosms’, summarizing all the climatic, ecological and socio-economic processes going on in this area and allowing us to study them in a conceptually limited condition, although spatial differences will be accounted. MCFs represent also one of the drivers affecting both the structure and functioning of the coastal area in the Northern Adriatic Sea, having important biogeochemical, biological, ecological and socio-economic effects. The goal is to assess the potential role of MCFs in determining the functioning of the coastal ecosystem, also in relation of possible effects on ecological status and goods and services. MCFs could represent a “worked” example that could be extremely useful in relation to the MSFD implementation and definition of ecological status indicators. In fact, at present, MSFD indicators are still under development and their capability to provide information about ecosystem functioning is still under study, thus the project will contribute to these developments. Moreover, this could contribute to the definition of management objectives, identifying the self-sustaining processes to be maintained or restored, in order to increase the system resilience and stimulate an adaptive management. The activity will be focused on the nektonic and benthic compartment, aiming to investigate the role played by mussels in contributing to the energy flows. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 31 New antimicrobial compounds produced by sponge-associated bacteria against opportunistic pathogens Conte A.1, Papale M.1, Rappazzo A.C.1, Fani R.2, De Domenico E1., Lo Giudice A.1 1 2 Department of Biological and Environmental Sciences, University of Messina. Department of Biology, University of Florence. The present research aims at finding new antimicrobial compounds against opportunistic pathogens (such as those involved in the cystic fibrosis) by bacteria isolated from Antarctic sponges. Sponge specimens were collected at Terra Nova Bay (Ross Sea) during the XXIX Italian Expedition to Antarctica (Austral Summer 2013-2014). The internal tissue of sponges was homogenized and serially diluted before plating on marine agar plates. Bacterial strains will be isolated from agar plates and then tested by the cross-streak method against different pathogenic bacteria (targets) and between themselves. Active isolates will be phylogenetically characterized by 16S rRNA gene sequencing and tested by the double plate method to evaluate the production of volatile organic compounds (VOCs). Thin layer chromatography will be applied to preliminarily characterize the antimicrobial compounds by using different standards, and the eventual VOCs will be analyzed by GC-MS. Moreover, active isolates will be physiologically characterized by testing their growth under different conditions (e.g. temperature, pH, carbon source) and the possible production of secondary metabolites will be investigated. Finally, bioinformatics analysis will be used to explore the genetic pathway that may be involved in the production of molecules with antibacterial activity. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 32 Phytoplankton distribution in lagoon ecosystems at different spatial scale Durante G.1, Roselli L.1, Stanca E.1, Basset A.1 1 Department of Biological and Environmental Sciences and Technologies, University of Salento, SP LecceMonteroni, 73100 Lecce, Italy. Tel.: +39 0832 298889; [email protected] Phytoplankton is a very diverse and widely distributed group of solitary or colonial organisms, playing a crucial role in all aquatic environments since they are the foundation of aquatic food webs. Understanding the dynamics of phytoplankton populations and the mechanisms of coexistence of different species, living in environments with a limited amount of resources and sharing same requirements, has challenged phytoplankton community ecologists from 1961. Geographical variations in environmental factors, affecting phytoplankton species coexistence at local scale, are responsible of species diversity at global scale. An important goal to understand the mechanisms that structure phytoplankton communities is to describe the spatial distribution patterns of organisms from local to global scale. Taxonomic composition and numerical abundance are two major components of every community, giving descriptive information on the organization of microalgae assemblages. A hierarchical sampling design was used to investigate variations in phytoplankton richness and composition in lagoon ecosystems at different spatial scale. The principal results are the following: taxonomic composition varies among ecosystems and geographic areas; however, even if everything is not everywhere, the overall taxa richness at different spatial scale, generally, does not show strong variations. These results suggest that: 1. the diversity of phytoplankton species at different spatial scale could be driven by environmental factors, acting as a filter, which selects the diversity from a common species pool and 2. the capacity of a system to support a number of species is more or less unvarying in lagoon ecosystems. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 33 How aquatic organisms cross the “dry ocean” and disperse between ponds? Incagnone G.1 1 Dipartimento di Scienze e Tecnologie Biologiche, Chimiche e Farmaceutiche – Sezione di Botanica ed Ecologia Vegetale - Università di Palermo - [email protected] Mediterranean temporary ponds (MTPs) are shallow water bodies characterized by the alternation of flooded and dry phases; this hydrological trait reflects the semi-arid climatic conditions of Mediterranean area. The dry period is an extreme ecological condition for aquatic organisms and, their biological communities have developed exclusive adaptations to survive. Actually, temporary pools support a high biodiversity. In spite of Habitats Directive 92/43/EEC, MTPs are threatened by human impacts and they are disappearing at a very fast rate This trend strengthen their geographical isolation, and may enhance the local extinction of species. The populations inhabiting MTPs are actually small, isolated and highly fluctuating in numbers. When the water temperature increases, these aquatic organisms generally produce drought-resistant structures (e.g. seeds, oospores, cysts) which can be dispersed by wind, birds, mammals. Dispersion and colonization are the mechanisms through which the conservation of a gene flow among populations occurs, thus contributing to biodiversity preservation. The aim of my research work is to investigate the mechanisms through which the different organisms (e.g. phyto- and zooplankton, macroinvertebrates, macrophytes) spread, in order to give a contribution to the protection of these peculiar environments. In the first year an exhaustive literature search will be carried out to review the status of the art of the knowledge on dispersal and colonization. Then, a few target species (e.g. Isoetes velata, Hemidiaptomus sp., Chara sp. and a few common airborne algae) will be selected and their dispersal abilities and distribution patterns as well as their phylogeography will be comparatively studied. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 34 Influence of inter-specific horizontal interactions and biodiversity on the plant detritus decomposition: an experiment in laboratory mesocosms Mazzotta L.1, Gjoni V.1, Marini G.1, Basset A.1, Pinna M.1 1 Department of Biological and Environmental Sciences and Technologies, Ecology Unit University of Salento, 73100 Lecce, Italy. Tel.: +39 3209771586, [email protected] Studying the relationship between structures and functions in natural ecosystems is one of the most relevant ecological topics. In the last decades has been paid attention to how the mechanisms of community organization and coexistence between species can influence the ecosystem functioning. In the formulation of the niche theory, the competitive relations and coexistence are measured in terms of overlap in the use of limiting resources. Fundamental theories emphasize the role of biodiversity and body-size in the community organization and in the mechanisms of resource partitioning among individuals, population and species (e.g., niche theory, lottery competitive, body-size theory, metabolic theory). In this research we analyzed: i. the role of benthic macroinvertebrate biodiversity on the functional process of plant detritus decomposition comparing experimental conditions without benthic macroinvertebrates (control), mono-specific bi-specific and three-specific conditions; ii. the role of benthic macroinvertebrate inter-specific competition on the functional process of plant detritus decomposition comparing bi-specific conditions characterized by different body-size ratio between species. The experiment was conducted by varying the temperature conditions, in order to correlate the process of plant detritus decomposition, biodiversity of benthic macroinvertebrates and different environmental conditions. Four species Lymnaea truncatula (Muller), Physa acuta (Draparnaud), Gammarus aequicauda (Martynov), Proasellus coxalis (Dollfus), collected in a slightly aquatic habitat, were used in mono-specific and in six bi-specific experimental conditions to obtain a gradient of increasing biodiversity and competitive pressures due to body-size overlapping. The experimental design and the results will be reported in detail in the poster. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 35 Study of the trophic and functional organization of fish assemblages in submarine shallow CO2 vents Mirasole A.1, Vizzini S.¹ 1 S Department of Earth and Marine Sciences, University of Palermo, via Archirafi 18, 90123 Palermo (ITALY) tel.: +39.091.23862877- fax: +39.091.6100278 [email protected] Naturally acidified marine ecosystems are currently investigated to evaluate the effects of ocean acidification on species, communities and ecological processes. Among these, submarine shallow CO2 vents represent a sort of natural mesocosms, where at small spatial scales marked gradient of pH are present. The aim of this project is to study the responses of fish assemblages to pH reduction in Mediterranean shallow CO2 vents by using several descriptors such as community structure, trophic organization, chemical characteristics and composition of carbonic structures. Moreover as in CO2 vents concentration and bio-availability of several trace elements, also toxic ones, may increase due to direct input from the vents and/or to peculiar pH and Eh conditions, another aim is the evaluation of trace element bioaccumulation and biomagnification. As scant information is available in the literature on this topic, the results of this research will contribute to bridge the gap to predict the ecological effects of ocean acidification on fishes, a key biological component whose monitoring is relevant not only from the ecological standpoint but also for the economic one and for the implications on human health. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 36 Spatial and temporal variation in foraging behavior of New Zealand fur seals (Arctocephalus forsteri) using archival tissues Noè S.1, Willis T. J.2, McGill R. A. R.3 1 Laboratorio di Ecologia Marina, Dip.to di Scienze della Terra e del Mare (DiSTeM), Università di Palermo, Italy, email: [email protected], Tel.: 320-9787935 2 School of Biological Sciences, University of Portsmouth, UK 3 NERC Life Sciences Mass Spectrometry Facility, Scottish Universities Environmental Research Centre, University of Glasgow, UK Spatial and temporal variation in foraging strategies of New Zealand fur seals (Arctocephalus forsteri) were investigated from three colonies (Kaikoura: South Island east coast, Tasman Bay: South Island north coast, and Cape Foulwind: South Island west coast) between December 2010 and June 2012, using the analysis of stable isotope ratios of carbon and nitrogen (δ13C and δ15N) of sampled whiskers. We used the isotopic values of basal elements (1 mm sections cut close to the skin) of the facial vibrissae to identify intra- and inter- colony variation in fur seal trophic level. We describe the range in isotope values in foraging lactating females, and differences among colonies that are likely to be a consequence of differences in locally available marine resources. The trophic level of mature female seals is similar to that of carnivorous reef-associated fishes, supporting diet analyses from scats that indicate diet to be primarily arrow squid (Notodarus sloanii) and small schooling fishes. There was no evidence that females compete with fishers for commercially exploited species: the mean value of δ15N was 14.10- 16.84 ‰ (equivalent to predatory reef fishes). There was some isotopic evidence of geographic separation of colonies (mean δ13C for Kaikoura = -15.6, Cape Foulwind = -16.3, Tonga Is = -16.7) indicating that females have limited foraging ranges and that there is limited interchange of individuals among colonies. At Cape Foulwind, δ15N values varied significantly between seasons, with a January (summer) mean of 16.03 ‰ ± 0.11 (SE), and June (winter) mean of 15.09 ‰ ± 0.17 (SE). Differences in trophic level between males, females and pups at Kaikoura were found, with suckling pups having δ15N values considerably higher (2.35 ‰) than females, reflecting pup’s consumption of maternal milk. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 37 Polychlorinated biphenyl degrading bacteria from polar environments Papale M. 1, Conte A. 1, Rappazzo A. C. 1, De Domenico E. 1, Lo Giudice A.1 1 Department of Biological and Environmental Sciences, University of Messina This research is aimed at finding bacterial strains able to degrade persistent pollutants such as polychlorinated biphenyls (PCBs) with a focus on the metabolic pathways underlying the process. Samples of water and sediment were collected from four different polar sites: 1) Byers Peninsula (Antarctica), 2) Terra Nova Bay (Antarctica) 3) High Arctic (Svalbard Islands) and 4) Norwegian Arctic. Antarctic and Arctic bacteria were preliminarily screened for their ability to use PCBs as the sole carbon and energy source at both 4 and 15°C. Results from the screening showed that the 4% of strains were able to grow in the presence of PCBs at almost one temperature tested. Selected strains will be identified by 16S rRNA gene sequencing and their biodegradation potential will be evaluated by gas chromatographic analysis under different conditions (e.g. temperature, salinity, pH). The occurrence of functional genes for PCB biodegradation, in particular the gene bphA, will be determined. Finally, natural samples (i.e. water and sediment) will be also analyzed for PCB concentration and functional gene occurrence. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 38 Functional and molecular diversity of the bacterial community associated to the invasive green alga caulerpa racemosa (chlorophyta, caulerpaceae) Rizzo L.1, Stabili L.1,2,3, Alifano P.1, Fraschetti S.1 1 Dipartimento di Scienze e Tecnologie Biologiche ed Ambientali, Università del Salento, Via Prov.le Lecce Monteroni, Lecce. [email protected] 2 Dipartimento di Scienze Ambientali, Informatica e Statistica, Università Ca’ Foscari di Venezia. 3 Istituto per l’Ambiente Marino Costiero, U.O.S. di Taranto, CNR, Via Roma 3, Taranto. Over 900 species have been reported as non-indigenous species in the Black and Mediterranean Seas. Among them the green alga Caulerpa racemosa (Forsskal) J. Agardh is an invasive species driving critical changes in Mediterranean native benthic habitats. In the present study a survey was undertaken in three sampling sites of Apulia (Italy, Mediterranean Sea) to analyse and compare the bacterial community profiles associated to the surface of C. racemosa. We used the Biolog system-Ecoplates™ (Biolog, Hayward, CA, USA) and polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) to analyse the functional and phylogenetic diversity of the algal associated bacterial assemblages. Culturable heterotrophic bacteria at 22 °C showed a mean value of 2.4 x 105 CFU mL-1 at Torre Guaceto (Italy), 2.2 x 105 CFU mL-1 at Otranto (Italy), 1.4 x 105 CFU mL-1 at Porto Cesareo (Italy). The analysis of the metabolic profiles revealed a similar metabolic diversity in the C. racemosa inocula, containing 18 communal carbon sources consumed in up to 72 h of incubation. The Ez-Taxon analysis of the DNA sequences of SSCP mutual bands demonstrated that there is a distinct group of bacteria (i.e. the genera Shewanella, Marinobacter, Vibrio, Granulosicoccus, unknown Bacteroidetes and unknown Rhodobacteraceae) consistently present on C. racemosa. Our results show that C. racemosa surface might host a specific bacterial community, possibly playing a crucial role for the algal spreading. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 39 Sessione 2 Eco-biologia dei sistemi acquatici X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 40 Molecular biomarkers development and genotoxicity study in talitrus saltator (crustacea, amphipoda) in the evaluation of coastal ecosystems quality Di Donato G.1, Ronci L.1, Setini A.1, De Matthaeis E.1 1 University of Rome “La Sapienza”, Department of Biology and Biotechnology “Charles Darwin”, Viale dell'Università 32, 00185 Rome. This research program involves investigations at different levels: ecological, ecotoxicological and molecular about biotic effects, associated with different types of environmetnal perturbations in sandy beaches. The aim of this study is to provide innovative indicators of environmental stressors, particularly, to highlight the impact of rivers on these ecosystems, in areas close to their mouths. A large number of toxic substances are introduced into marine environment by industrial, agricultural and other anthropogenic activities. In the last decades, the increasing contamination of water bodies, especially in coastal areas has required a deeper knowledge of the environment diffusion of these substances. Environmental quality of sandy beach ecosystems is strongly influenced by chemical property of coastal waters, determined in large part by the release of toxic substances through rivers. The complex nature of coastal environments has led, over time, to the development of ecosystems characterized by specialized and highly adapted communities exclusively present in these habitats. Talitrid amphipods constitute the main animal biomass in sandy beach ecosystems and play an important role in the energy flow among different trophic levels. The destruction of natural habitats by rapidly expanding of human populations often brings to a simplification of the diversity of these habitats. Hence, is prioritary understood the answers of these communities to chemical stress and develop appropriate programs to preserve beach systems. At this purpose, monitoring programs based on biological indicators aims to establish a link between the presence of chemical compounds and their effects on biota. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 41 Sessione 3 Ecotossicologia ed Ecologia Microbica X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 42 Paleoclimatic and Paleoceanographic reconstruction of the Pleistocene – Holocene through the study of planktonic foraminifera of two sediment cores, collected in the Atlantic Ocean, southwestward of the Azores Islands (OCEANOGRAFLU Geological survey, June 2013) Bonfardeci A. 1 1 Earth and Marine Science, University of Palermo. +39 3294790636, [email protected] The study of climate and oceanographic setting in the recent past plays and important role to prevent what we can expect for the future. This research project is based on the study of two sedimentary cores (ATA13-OF-KT1 and ATA13-OF-KT18), collected during OCEANOGRAFLU 2013 oceanographic survey in Atlantic Ocean, southwestward of the Azores Islands, near the Mid-Atlantic ridge. In agreement with members of Paleobiodiversity and Paleoenvironmental Research Center belonging to the Department of Earth History (MNHN) of Paris, I’ll have to study these two cores, under the supervision of Prof. Annachiara Bartolini, member of this research unit, and Prof. Antonio Caruso (DiSTeM, University of Palermo). This research project is based on the analysis of percentages of abundance of planktonic foraminiferal assemblages, contained in these sedimentary cores. The aim is to establish the combined effects of climatic changes and ridge activity on planktonic foraminiferal assemblages, during the Upper Pleistocene – Holocene. These two cores will be sliced every cm and the obtained samples will be analyzed in order to recognize the composition of planktonic foraminiferal assemblages and to estimate the fluctuation in abundance of each species, by counting of 300 specimens for sample. Furthermore geochemical analysis will be performed, regarding: • Mg/Ca and Sr/Ca calcite ratios of foraminiferal shells; • Isotopic composition of foraminiferal shells, regarding δ18O and δ13C; • Radiometric dating (14C) to define a correct “age-model”. All these data will be correlated to carry out a paleoclimatic and paleoceanographic reconstruction and in order to obtain an “event stratigraphy” of this sector of the Atlantic Ocean, during the Upper Pleistocene – Holocene. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 43 Toxin-producing cyanobacteria in the large lakes south of the Alps: detection of new producers and molecular identification methods Capelli C.1,2, Shams S1,3., Cerasino L.1, Cavalieri D.1, Papini A.2, Salmaso N.1 1 Research and Innovation Centre, Fondazione Edmund Mach, Via E. Mach 1, 38010 San Michele all’Adige, Italy. Tel.: +39 0461 615548; E-mail address: [email protected] 2 Department of Biology, Universita` di Firenze, Via La Pira, 4, 50121 Firenze, Italy 3 Human and Environmental Toxicology Group, Department of Biology, University of Konstanz, P.O. Box X918, D-78457, Konstanz, Germany Cyanobacteria produce a huge variety of secondary toxic metabolites that can cause serious health problems in humans and animals. Cyanotoxins include hepatotoxins (e.g., microcystins, MCs), neurotoxins (e.g., anatoxin-a, ATX) and endotoxins. The general aim of the project is to identify, at the species and strain level, cyanobacteria producing toxins, especially anatoxins, in Lake Garda and in the other large and deep lakes south of the Alps. The dominant cyanobacteria in these lakes are represented by Planktothrix rubescens, which is responsible of the production of MCs. Nevertheless, in the last two decades there was a rapid spread of an invasive species, Dolichospermum lemmermannii, which is able to synthesize both MCs and neurotoxins (ATX). This appearance raised questions linked to the potential production of new toxins. Recent research showed significant concentrations of ATX, which, most of the time, were not matched by the presence of sizeable populations of D. lemmermannii, posing problems about the identification of the toxin producers. This emerging risk makes necessary to analyse in detail the causes of ATX production. First we will evaluate toxin concentrations and the dominant toxic cyanobacteria in environmental samples. Secondly we will use metagenomics to study the complexity of the cyanobacterial communities. Hereafter, using a molecular approach, based on high throughput DNA and mRNA sequencing we will detect the presence of ATX encoding genes, both in whole environmental samples and isolated strains. In this last we will even investigate the cell ultrastructure to get further data about the ATX production mechanisms. This multidisciplinary approach will allow to identify potential new anatoxin producers and different toxin encoding genes, as well as to unravel the community structure and the ecological determinants triggering anatoxin production. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 44 CO2 effects on behaviour and reproduction of nesting fish species Cattano C. 1 1 Dipartimento di Scienze della Terra e del Mare (DiSTeM), University of Palermo, Via Archirafi, 28 – 90123 Palermo, Italy. [email protected] Ocean acidification is a global phenomenon linked to the CO2 absorption by the surface of the sea. This increasing process leads to a rise of the CO2 partial pressure (pCO2) that changes the seawater chemistry. It is know that such changes have negative consequences for many marine organisms. However, the effects of acidification on fishes are yet poorly understood. Fishes have been hypothesized to be more physiologically tolerant to elevated CO2. Nevertheless, many researches have described severe effects of altered pCO2 levels on fish behaviour, especially during early life history stages, and only few authors have focused on fish reproduction. The aim of my Ph.D. research is to investigate the effects of different pCO2 levels on the reproduction of nesting fish species, with particular emphasis on reproductive behaviour and reproductive success, and on early life stages development. This research will be conducted in the Vulcano Island coast where a natural gradient of pH and CO2 exists, due to underwater volcanic emissions dominated by CO2. Preliminary observations on fish community composition in this place have shown different distribution patterns of the species along the pCO2 gradient. In particular, some species belonging to genus Symphodus display the same distribution pattern in high pCO2 levels (= ca. 1100 µatm) and in normal pCO2 levels (= ca. 400 µatm). The males of these species build elaborate nests in which several females spawn, and they could be more sensitive to the acidification effects due to territorial habits shown during their reproductive period. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 45 Cross-community scaling of benthic macroinvertebrate guilds in freshwaters of Southern Italy Gjoni V. 1, Mazzotta L.1 , Marini G.1 , Pinna M.1 , Basset A. 1 1 Department of Biological and Environmental Sciences and Technologies, University of Salento, SP Lecce-Monteroni, 73100, Lecce, Italy. [email protected] The most fundamental property of an organism is the body size because it is related to many biological traits, as well as to population abundance and community level characteristics. Many studies have focused on the relationships between abundance and body size in an attempt to quantify the form of these relationships and to understand the processes and mechanisms underlying them. One way to address the relationship between individual body size and abundance at the community level is represented by the cross-community scaling relationship (CCSR), i.e., the relationship between overall density and average individual body size at the community level. In general, CCSRs tend to be well described by power laws where the exponent b tends to be similar to the inverse of the scaling exponent of metabolic rates (b= -0.75). In other words, power law CCSRs are expected to result simply from basic energy balance arguments such as zero-sum competition for limiting resources or space. Field experiments on freshwater benthic macroinvertebrate guilds of Southern Italy (Sardinian rivers) were designed to test the cross-community scaling relationship relevance of body size-related constraints on the organization of detritus-based benthic guilds through the relationship between the average size of an individual in an assemblage and the total community density. The first results are going to be presented in the poster. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 46 Investigations into the ecosystem service value of intertidal bioconstructions in the context of coastal erosion and rock breakdown La Marca E. C.1 1 Department of Earth and Sea Sciences, University of Palermo, Via Archirafi 28, 90123 – Palermo. Tel. +39 091 23862864; email: [email protected] Coastal environment is characterized by the presence of some of the most productive and valued habitats in the world. The lack of knowledge about the ecosystem service provided by these habitats is a limit to the application of the principles of environmental management. The purpose of this research project is to study the ecosystem services supported by coastal bio-constructions, specifically vermetid reefs and mussel beds, against the deterioration of rocky coast. The main hypothesis is that the presence of vermetid reefs and mussel beds partially isolates the underlying rocks from erosive agents and protects them from breakdown processes. The questions which this research aims to answer are: 1) Is it possible to assign a “bio-protective role” to the organisms involved in coastal bioconstructions? 2) If so, what is the value of the ecosystem service provided by vermetid reefs and mussel beds against rock deterioration? 3) Could these species be used such as mechanisms of defense against coastline erosion? If so, how can we encourage their colonization and growth? In order to elucidate the “bioprotective” role of vermetids and mussels and to establish their influence on weathering and erosion processes, the research will carry out controlled laboratory experiments and field monitoring and sampling. Shedding light on these three points would be of relevance in the evaluation of eco-sustainable choices to protect the coastline from deterioration and recession, and, at the same time, it would improve the management and conservation of the considered habitats, as suggested by the international politics of environmental sustainability. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 47 Coccolithophore calcification response to ocean acidification: Vulcano Island, southern Italy as a natural laboratory Napoli S.1 1 Dipartimento di Scienze della Terra e del Mare, Università degli Studi di Palermo, via Archirafi 22/28, 90123 Palermo, Italy. Tel: 3356012610, E-mail: [email protected] More than a third of CO2, produced by human activity and emitted into the atmosphere, is absorbed by the oceans. The main consequence of this phenomenon is the seawater pH lowering that may affect both biotic and abiotic processes. Ocean acidification modifies carbonate chemistry and changes community structure and biodiversity. So far most of investigations deal with benthic organisms that exhibit reduced or abnormal calcification, in response to elevated concentration of CO2. Only a few culture experiments have been carried out to study planktonic calcifier groups. The aim of this PhD project is the study of coccolithophores collected at Levante Bay (Vulcano Island, southern Italy), where an intense CO2 emission is due to volcanic vents. This area represents an important natural laboratory to study planktonic assemblage variations across gradients that parallel the projected pH decrease in the near future. Living coccolithophores will seasonally be sampled and examined in terms of cell concentration, species diversity, coccoliths malformation and dissolution. Results will be compared to seawater physical and chemical properties, among others pH and total alkalinity. In addition, analysis on trace elements will be carried out in order to assess the possible influence of toxic metals on calcification processes. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 48 TROPTUNA: Global population structure and maternal effect in bigeye tuna (Thunnus albacore) Pecoraro C.1,2,Cariani A.1, Bodin N.2, Chassot E.2, Zudaire I.2, Tinti F.1 1 Università di Bologna Institut de recherche pour le développement (IRD) 2 Tropical tunas (TT) account for the greatest part of world tuna catches but their stock biomass declined ca. 60% from 1954 to 2006, increasing the risk of ecological/economic extinctions. Nowadays the population structure of these species is still poorly understood and they have been considered to be highly mobile and consist of a single panmictic spawning population for the purposes of stock assessment and management. My research project “TROPTUNA” was born from the scientific collaboration between the University of Bologna (Prof. Fausto Tinti) and the French Research Institute for the Development (IRD). The TROPTUNA project intend to analyze the genomic variation across Thunnus obesus (BET) populations worldwide, through an advanced population genomic approach based on the Next Generation Sequencing technologies. In order to reveal population and stock structure and to improve the general understanding of ecological and evolutionary responses of BET stocks to environmental changes and to fisheries. According to previous studies that indicated the presence of at least two different Clades in Atlantic and Indian oceans, the second main objective of TROPTUNA is to point out the reproductive dynamics related to the energy allocation in BET spawning females of CE Atlantic and CW Indian populations, addressing the maternal effect hypothesis for BET. In fact despite the recognition of the importance of accounting for maternal effect into fisheries management, no study has been conducted until now to identify and quantify maternal effect for TT in order to evaluate the effects of size-selective catches on the sustainability of their stocks. X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 49 INDICE ALFABETICO DEI PRIMI AUTORI Andolina C. Using food web metrics as descriptors to evaluate variation of trophic organization at spatial and temporal scales 30 Azzella M.M. Influence of water transparency and lake morphology on the distribution of macrophytes along the depth gradient 16 Bonfardeci A. Paleoclimatic and Paleoceanographic reconstruction of the Pleistocene – Holocene through the study of planktonic foraminifera of two sediment cores, collected in the Atlantic Ocean, southwestward of the Azores Islands (OCEANOGRAFLU Geological survey, June 2013) 43 Canepa S. Application of innovative methods to evaluate the ecotoxicity of Diethylene glycol 23 Capelli C. Toxin-producing cyanobacteria in the large lakes south of the Alps: detection of new producers and molecular identification methods 44 Carrozzo L. Infection of native crab species and of the invasive Callinectes sapidus by the dynoflagellate Hematodinium spp. in two Mediterranean coastal habitats 17 Cattano C. CO2 effects on behaviour and reproduction of nesting fish species 45 Colla S. The role of offshore mussel culture farms in relation to the structure and function of the adriatic coastal ecosystem 31 Conese I. Impacts of dense shelf water cascading in the southern Adriatic Sea: an integrated approach to evaluate small-scale variability on the benthic compartment 18 Conte A. New antimicrobial compounds produced by sponge-associated bacteria against opportunistic pathogens 32 D'Alessandro M. Study of the macrozoobenthic community within the Grand Harbour, Valletta, Malta 9 Di Donato G. Molecular biomarkers development and genotoxicity study in talitrus saltator (crustacea, amphipoda) in the evaluation of coastal ecosystems quality 41 Durante G. Phytoplankton distribution in lagoon ecosystems at different spatial scale 33 Fornaroli R. Quantile regression analysis macroinvertebrate biodiversity 10 as predictive X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 for lake 50 Giussani V. Insights on the toxic effect of Ostreopsis cf. ovata: the role of direct/indirect contact with microalgal cells 24 Gjoni V. Cross-community scaling of benthic macroinvertebrate guilds in freshwaters of Southern Italy 46 Incagnone G. How aquatic organisms cross the “dry ocean” and disperse between ponds? 34 Ingrosso G. Relationship between seasonal variation of carbonate system and coccolithophores in the gulf of Trieste 19 Innangi S. MultiBeam EchoSounder (MBES) backscatter signal analysis in marine environment: Applications to study 3D pelagic fish resources 20 La Marca E.C. Investigations into the ecosystem service value of intertidal bioconstructions in the context of coastal erosion and rock breakdown 47 Mangano M.C. Effects of chronic bottom trawling disturbance on benthic communities in the Southern Tyrrhenian Sea 11 Marazzi F. Growth of microalgal biomass on supernatant from biosolids dewatering 25 Mazzotta L. Influence of inter-specific horizontal interactions and biodiversity on the plant detritus decomposition: an experiment in laboratory mesocosms 35 Milan M. Environmental drivers for the secular evolution of Lake Garda as outlined by paleolimnological reconstructions 21 Mirasole A. Study of the trophic and functional organization of fish assemblages in submarine shallow CO₂ vents 36 Morgana S. Development of new biosensing techniques to monitor marine pollution 26 Napoli S. Coccolithophore calcification response to ocean acidification: Vulcano Island, southern Italy as a natural laboratory 48 Nasi F. Response of macrozoobenthos to synergistic contaminants in the Mar Piccolo of Taranto of 12 Noè S. Spatial and temporal variation in foraging behavior of New Zealand fur seals (Arctocephalus forsteri) using archival tissues 37 Papale M. Polychlorinated environments 38 biphenyl degrading bacteria X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 effect from polar 51 Pecoraro C. TROPTUNA: Global population structure and maternal effect in bigeye tuna (Thunnus albacore) 49 Poma G. Evaluation of spatial distribution and accumulation of novel brominated flame retardants, HBCD and PBDES in an Italian subalpine lake using zebra mussel (Dreissena polymorpha) 27 Rizzo C. Biosurfactant production by bacteria isolated from filter-feeding organisms (Annelida:Polychaeta) 28 Rizzo L. Functional and molecular diversity of the bacterial community associated to the invasive green alga caulerpa racemosa (chlorophyta, caulerpaceae) 39 Rogelja M. Benthic microalgal community composition and trophic status of sediments in the port of Trieste 13 Visani I. A municipality, an ecosystem 14 X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 52 COMITATO ORGANIZZATORE Mattia M. Azzella La Sapienza, Università di Roma Edoardo Calizza La Sapienza, Univesità di Roma Camilla Capelli Fondazione E. Mach, Istituto Agrario di S. Michele all'Adige Dipartimento di Biologia - Università di Firenze Giulia Incagnone Università di Palermo Manuela Milan Fondazione E. Mach, Istituto Agrario di S. Michele all'Adige Dipartimento di Ecologia e Scienze Ambientali - Università di Umeå (Svezia) Sajid Pareeth Fondazione E. Mach, Istituto Agrario di S. Michele all'Adige Immagini di Andrea Cattani X Incontro dei dottorandi in Ecologia e Scienze Ambientali, Università di Palermo, 07 – 10 Aprile 2014 53
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