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Chemi-Lumi One

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L‫ڠا‬อ࢕࠿੄΅ΛΠ
87
ήυΛ΅ϋΈဣ‫ס‬
Chemi-Lumi One࡮Blocking One
Chemi-Lumi One
䊒䊨䊁䉥䊷䊛⎇ⓥ䈏⋓䉖䈮ዷ㐿䈘䉏䈩䈇䉎੹ᣣ䇮᛫ේ᛫૕෻ᔕ䉕೑↪䈚䈢఺∉ቇ⊛ᚻᴺ䈮䉋䉎䉺䊮䊌䉪⾰䈱․⇣⊛ᬌ಴䊶หቯ䈲䇮䊋䉟䉥䊁
䉪䊉䊨䉳䊷ಽ㊁䈮䈍䈇䈩ၮ⋚䈎䈧ᔅⷐਇนᰳ䈭ᛛⴚ䈫䈭䈦䈩䈇䉁䈜䇯ਛ䈪䉅䇮㔚᳇ᵒേᓟ䈱䉺䊮䊌䉪⾰䉕䊜䊮䊑䊧䊮਄䈮ォ౮䈚䈢ᓟ䇮X✢䊐䉞
䊦䊛䈮ኒ⌕䈘䈞䇮ᓸ㊂䉺䊮䊌䉪⾰䉕․⇣⊛䈎䈧㜞ᗵᐲ䈮ᬌ಴䈜䉎ൻቇ⊒శᬌ಴ᛛⴚ䈲䇮ᦨ㜞ፄ䈱ᬌ಴ᚻᲑ䈪䈅䉍䇮ᦨ䉅᳢↪ᕈ䈱㜞䈇ᬌ಴
ᛛⴚ䈪䈜䇯ㄭᐕ䇮⍴ኼ๮䈪䈅䈦䈢ൻቇ⊒శ䈏ᛛⴚᡷ⦟䈘䉏㐳ኼ๮ൻ䈚䇮䊑䊨䉾䊁䉞䊮䉫ᬌ಴䈭䈬䈮ᔕ↪䊶᳢↪䈘䉏䈩䈇䉁䈜䇯䈖䈖䈪䈲ᒰ␠䈪
⽼ᄁਛ䈱ൻቇ⊒శᬌ಴䉨䉾䊃䉕⚫੺䈚䉁䈜䇯
․˴㐳
٨ჺ଺᧓Ὁ᭗ज़ࡇ౨Јỉ҄‫ܖ‬ႆήỿἕἚ
٨˯ẟἢἕἁἂἻỸὅἛỂᅵ᡹ễἙὊἑ౨Ј
⵾ຠ⺑᣿
ᬌ಴ේℂ㩿ㅢᏱᬌ಴㪀
ᬌ಴ේℂ㩿㜞ᗵᐲᬌ಴㪀
䋨䉰䊮䊄䉟䉾䉼ᴺ䋩
䋨䉝䊎䉳䊮㪄䊎䉥䉼䊮♽೑↪䋩
ㆊ㉄ൻ‛
Luminol
⊒శ
ㆊ㉄ൻ‛
Luminol
ൻቇ⊒శ䈱෻ᔕ䊜䉦䊆䉵䊛
⊒శ
ࠛࡦࡂࡦࠨ࡯
ࠛࡦࡂࡦࠨ࡯
21&
ࡆࠝ࠴ࡦ21&
ᰴ᛫૕
ᰴ᛫૕
㧔21&ᮡ⼂᛫૕㧕
ᰴ᛫૕
㧔ࡆࠝ࠴ࡦ᛫૕㧕
ࠕࡆࠫࡦ
ᰴ᛫૕
࠲ࡦࡄࠢ⾰
࠲ࡦࡄࠢ⾰
⊒శ
䊦䊚䊉䊷䊦䋨Luminol䋩෻ᔕ䈫䈲䇮䊦䊚䊉䊷䊦䈫䈇䈉ൻว‛䈏㉄ൻ䈘䉏䈩䇮3-䉝䊚䊉䊐䉺䊦㉄䈮䈭䉎෻ᔕ䈪䈜䇯
䊕䊦䉥䉨䉲䉻䊷䉷䈲䇮ㆊ㉄ൻ᳓⚛䈫䊦䊚䊉䊷䊦䈱෻ᔕ䉕⸅ᇦ䈚䇮㕍⦡䈱Ⱟశ‛⾰3-䉝䊚䊉䊐䉺䊦㉄䉕↢ᚑ䈚䉁䈜䇯
૶↪ᣇᴺ
ㅢᏱᬌ಴ᴺ䊶㜞ᗵᐲᬌ಴ᴺ᭎⇛࿑
㔚᳇ᵒേ䊶䊑䊨䉾䊁䉞䊮䉫
䊑䊨䉾䉨䊮䉫
(BlockingOne૶↪)
1ᰴ᛫૕෻ᔕ
ㅢᏱᬌ಴ᴺ
㜞ᗵᐲᬌ಴ᴺ
PODᮡ⼂᛫૕෻ᔕ
䊎䉥䉼䊮ᮡ⼂᛫૕෻ᔕ
䉴䊃䊧䊒䊃ABC
䊕䊦䉥䉨䉲䉻䊷䉷䉨䉾䊃෻ᔕ
Chemi-LumiOne
⊒శ↪ᷙวᶧ෻ᔕ
ᗵశ䊶⃻௝䊶ቯ⌕
૶↪ᣇᴺ
1
㽲 䉰䊮䊒䊦䇮䊎䉥䉼䊮ᮡ⼂䉺䊮䊌䉪⾰䊙䊷䉦䊷* (⵾ຠ⇟ภ䋺02886-44)䋨䉅䈚䈒䈲㕖䊎䉥䉼䊮ᮡ⼂
䉺䊮䊌䉪⾰䊙䊷䉦䊷䋩䉕㔚᳇ᵒേᓟ䇮䉭䊦䉕⤑䈮ォ౮䈚䇮0.05䋦 Tween 20฽᦭TBS(t-TBS)䉕↪䈇
䈩⤑䉕ᵞᵺ䈚䉁䈜䇯(5ಽ㑆㬍2࿁)
㽳 䊑䊨䉾䉨䊮䉫䊪䊮*3 (⵾ຠ⇟ภ䋺03953-95)䉕↪䈇䈩45ಽ㑆 ቶ᷷䈪䊑䊨䉾䉨䊮䉫ಣℂ䉕ⴕ䈇䉁䈜䇯
㽴 t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯(5ಽ㑆㬍2࿁)
㽵 1ᰴ᛫૕䉕෻ᔕ䈘䈞䉁䈜䇯(ቶ᷷䇮1ᤨ㑆)
㽶 t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯(5ಽ㑆㬍2࿁)
2
㽷 䊎䉥䉼䊮ᮡ⼂᛫૕ (2ᰴ᛫૕)* (䉅䈚䈒䈲PODᮡ⼂᛫૕)䉕෻ᔕ䈘䈞䉁䈜䇯(ቶ᷷1ᤨ㑆)
2
㽸 t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯(5ಽ㑆㬍2࿁)*
㽹 䉴䊃䊧䊒䊃ABC䊕䊦䉥䉨䉲䉻䊷䉷䉨䉾䊃 (⵾ຠ⇟ภ䋺30462-30)䈱Working Solution䉕⺞⵾䈚䇮
2
t-TBS䈪10୚Ꮧ㉼䈚䈢ṁᶧ䉕෻ᔕ䈘䈞䉁䈜䇯*
㽺 t-TBS䉕↪䈇䈩⤑䉕ᵞᵺ䈚䉁䈜䇯(5ಽ㑆㬍2࿁)
㽻 Solution A䈫Solution B䉕1䋺1䈱ഀว䈪ᷙว䈚䉁䈜䇯 (ᷙวṁᶧ䈲䊑䊨䉾䊁䉞䊮䉫䈚䈢⤑䈮ኻ䈚
䈩⚂ 0.125ml/cm2䈮䈭䉎㊂䉕૶↪䈚䉁䈜䇯)
㽼 䊒䊤䉴䉼䉾䉪⵾䊤䉾䊒䊐䉞䊦䊛䉕᧍਄䈮ᢝ䈐䇮䈠䈱਄䈮䊑䊨䉾䊁䉞䊮䉫⤑䈱䉺䊮䊌䉪⾰ๆ⌕஥
䈏਄஥䈮䈭䉎䉋䈉䈮⟎䈐䉁䈜䇯
㽽 0.125ml/cm2䈱⊒శ↪ᷙวᶧ䉕⤑䈮タ䈞䇮1ಽ㑆෻ᔕ䈘䈞䉁䈜䇯
㽾 ⤑䈱┵䉕䉨䊛䊪䉟䊒䈮⌕䈔䉎䈭䈬䉕䈚䈩䇮૛ಽ䈭ᷙวᶧ䉕ข䉍㒰䈐䇮⤑䉕චಽ䈮൮䉄䉎⒟ᐲ䈱
ᄢ䈐䈘䈱ᣂ䈚䈇䊒䊤䉴䉼䉾䉪⵾䊤䉾䊒䊐䉞䊦䊛䈮䉺䊮䊌䉪⾰ๆ⌕஥䈱㕙䈏ਅ஥䈮䈭䉎䉋䈉䈮
䊑䊨䉾䊁䉞䊮䉫⤑䉕⟎䈐䉁䈜䇯 (䈖䈱ᤨ䇮⤑䈫䊤䉾䊒䊐䉞䊦䊛䈱㑆䈮᳇ᵃ䈏౉䉌䈭䈇䉋䈉䇮
䉁䈢䇮䊤䉾䊒䊐䉞䊦䊛䈱᛬䉏䉇䉲䊪䈏⴫㕙䈮಴᧪䈭䈇᭽䈮ᵈᗧ䈚䈩䈒䈣䈘䈇䇯)
㽿 䊤䉾䊒䊐䉞䊦䊛䈪䊑䊨䉾䊁䉞䊮䉫⤑䉕൮䉂䇮䉺䊮䊌䉪⾰ๆ⌕஥䈱㕙䉕਄䈮䈚䈩䊐䉞䊦䊛䉦䉶䉾
䊃䈮⟎䈐䇮䊐䉞䊦䊛䉦䉶䉾䊃䉕ᥧቶ䈮⟎䈐䉁䈜䇯
㾀 ᥧቶౝ䈪䇮䊤䉾䊒䊐䉞䊦䊛䈪൮䉖䈣䊑䊨䉾䊁䉞䊮䉫⤑䈱਄䈮X✢䊐䉞䊦䊛䉕⟎䈐䇮䊐䉞䊦䊛䉦
䉶䉾䊃䉕㐽䉄䇮3ಽ㑆ᗵశ䈘䈞䉁䈜䇯
㾁 X✢䊐䉞䊦䊛䉕⃻௝ᶧ䈮ẃ䈔䇮௝䈏⏕⹺䈪䈐䉎䉁䈪⃻௝䈚䉁䈜䇯
㾂 X✢䊐䉞䊦䊛䉕ቯ⌕ᶧ䈮ẃ䈔䇮௝䉕ቯ⌕䈘䈞䉁䈜䇯
㾃 X✢䊐䉞䊦䊛䉕ᥧቶ䈎䉌಴䈚䇮⚿ᨐ䉕⏕⹺䈚䉁䈜䇯
*1) 䊎䉥䉼䊮ᮡ⼂䊙䊷䉦䊷䉕೑↪䈜䉎႐ว䇮䉴䊃䊧䊒䊃ABC♽⹜⮎䈱૶↪䈲ᔅ㗇䈪䈜䇯
*2) ㍈ᢅ䈭ᬌ಴ᴺ䈫䈚䈩䈖䈱ᠲ૞䉕䈗ផᅑ⥌䈚䉁䈜䇯
㵘㵘PODᮡ⼂2ᰴ᛫૕䋨ㅢᏱ䈱䉰䊮䊄䉟䉾䉼ᴺ䋩䉕૶↪䈜䉎႐ว䈲䇮䈖䈱ᠲ૞䈱ᔅⷐ䈲䈅䉍䉁䈞䉖䇯
*3) 䊑䊨䉾䉨䊮䉫䊪䊮䈲䇮䉝䊎䉳䊮䊶䊎䉥䉼䊮෻ᔕ䉕೑↪䈚䈢ᬌ಴♽䈪䈲䇮ᧄᶧ䈱䉡䉲↱᧪䉺䊮䊌䉪⾰䈫
੤Ꮕ෻ᔕ䈜䉎น⢻ᕈ䈏䈅䉍䉁䈜䇯䉁䈢ᧄᶧ䈮฽᦭䈘䉏䈩䈇䉎䉺䊮䊌䉪⾰䈲䇮♖⵾䉺䊮䊌䉪⾰䉕૶↪䈚䈩
䈇䉁䈜䈏䇮᷹ቯ♽䈱᛫૕䉇㉂⚛ᮡ⼂᛫૕䈭䈬䈫੤Ꮕ෻ᔕ䈜䉎น⢻ᕈ䈏䈅䉍䉁䈜䇯૶↪䈮వ┙䈦䈩䇮
੤Ꮕ෻ᔕ䈱᦭ή䉕䈗⏕⹺䈒䈣䈘䈇䇯
૶↪଀
Chemi-Lumi One䉕↪䈇䈢ᬌ಴⚿ᨐ
Ԙ˴ԙԚԛ
%Ԝԝ
䊧䊷䊮㽲䌾㽷㩷䋺㵘2㱘g / 䊧䊷䊮
C
䋺㵘䉮䊮䊃䊨䊷䊦
: 10 㱘g or 20 㱘g of Drosophila Schneider-2
cell lysate
⤑
: PVDF⤑
ࡉࡠ࠶ࠠࡦࠣ : 5% dry milk / TBS, 30 min, RT
৻ᰴ᛫૕
: anti-Akt H136 (1/1000, Santa Cruz) /
0.25% BSA / TBS, 10 h, 4㷄
ᵞᵺ
: 0.5% Tween-20 / TBS, 10 min x 3, RT
ੑᰴ᛫૕
: anti-rabbit IgG, HRP-conjugated (1/2000) /
0.5% Tween-20 / TBS, 30 min, RT
ᵞᵺ
: 0.5% Tween-20 / TBS, 10 min x 5, RT
㔺శ
: 1 min
ࠨࡦࡊ࡞
࠺࡯࠲ߏឭଏ㧦᧲੩ㇺ⥃ᐥකቇ✚ว⎇ⓥᚲ⮎ℂ⎇ⓥㇱ㐷
˴˴˴˴˴˴˴೨ựᦺᒾవ↢
ࠨࡦࡊ࡞
⤑
ࡉࡠ࠶ࠠࡦࠣ
৻ᰴ᛫૕
ੑᰴ᛫૕
㔺శ
: ࡑ࠙ࠬ⣖᛽಴ᶧ
: ࠾࠻ࡠ࠮࡞ࡠ࡯ࠬ
: 5% ࠬࠠࡓࡒ࡞ࠢ/ 0.05% PBST
:anti-APP,Ctm
(1/3,000, overnight, 4͠)
:HRP-conjugated Anti Rabbit
(1/5,000, 1hr, RT)
:3 min
࠺࡯࠲ߏឭଏ㧦ℂൻቇ⎇ⓥᚲ˴
⣖⑼ቇ✚ว⎇ⓥ࠮ࡦ࠲࡯
˴˴
૶↪଀
䉝䊎䉳䊮㪄䊎䉥䉼䊮䉕↪䈇䈢㜞ᗵᐲᬌ಴
ԘԙԚ
ԛ
㽲䊎䉥䉼䊮ᮡ⼂䊙䊷䉦䊷
㽳䊙䉡䉴ⴊᷡ1000୚Ꮧ㉼:0.18㱘g/䊧䊷䊮
㽴䊙䉡䉴ⴊᷡ1000୚Ꮧ㉼:0.36㱘g/䊧䊷䊮
㽵䊙䉡䉴ⴊᷡ1000୚Ꮧ㉼:0.72㱘g/䊧䊷䊮
䊎䉥䉼䊮ᮡ⼂᛫䊙䉡䉴IgG᛫૕䈫෻ᔕᓟ䇮䉴䊃䊧䊒䊃ABC䊕䊦䉥䉨䉲䉻䊷䉷䉨䉾䊃෸䈶
Chemi-Lumi One䋨㔺శᤨ㑆䋺3 min䋩䉕↪䈇ᬌ಴䈚䉁䈚䈢䇯Ꮐ࿑䈱䊂䊷䉺䈎䉌䇮䉝䊎䉳䊮䊎䉥䉼䊮䉕↪䈇䈢ᬌ಴♽䈮䉋䉍䇮ᓸ㊂䈱䉺䊮䊌䉪⾰䉕㍈ᢅ䈮ᬌ಴䈜䉎੐䈏น⢻䈪䈜䇯
䊥䊒䊨䊷䊎䊮䉫䊂䊷䉺
ઁ␠⵾ຠ B Chemi-Lumi One
㽲 䊍䊃ⴊᷡ (1000୚Ꮧ㉼), 䊙䉡䉴ⴊᷡ(1000୚Ꮧ㉼)䈱ᷙว‛ 2.5, 5.0, 10.0 㱘l
䉕SDS䋭PAGE
㽳 䉶䊚䊄䊤䉟䊑䊨䉾䊁䉞䊮䉫 (PVDF⤑)
㽴 䊑䊨䉾䉨䊮䉫 (Blocking One)
㽵 ᛫䊙䉡䉴IgG᛫૕ (PODᮡ⼂)䈪෻ᔕ
㽶 ൻቇ⊒శᬌ಴
㽷 䊥䊒䊨䊷䊎䊮䉫 [Tris✭ⴣᶧ(67.5 mM, pH 6.8)(2䋦 SDS, 100 mM 2-䊜䊦䉦䊒䊃
㵘䉣䉺䊉䊷䊦฽᦭) ]70㷄, 45min
㽸 䊑䊨䉾䉨䊮䉫 (Blocking One)
㽹 ᛫䊍䊃䉝䊦䊑䊚䊮᛫૕ (PODᮡ⼂)䈪෻ᔕ
㽺 ൻቇ⊒శᬌ಴
਄࿑䈱䊂䊷䉺䈎䉌䇮Chemi-Lumi One䈲䊥䊒䊨䊷䊎䊮䉫䉕ⴕ䈦䈩䉅૶↪น⢻䈪䈅䉍,
䉁䈢ઁ␠⵾ຠ䈫㆖⦡䈱䈭䈇ᕈ⢻䉕␜䈜੐䈏䇮ታ⸽䈘䉏䈩䈇䉁䈜䇯
⵾ຠౝኈ
Solution A : 䊦䊚䊉䊷䊦ṁᶧ 250 ml
Solution B : ㆊ㉄ൻ‛ṁᶧ
250 ml
䊶ᧄ䉨䉾䊃䈮䈲⣣㘩ᕈ䈱‛⾰䈏฽䉁䉏䈩䈇䉁䈜䇯䈗૶↪䈱㓙䈮䈲ᚻⴼ䇮㒐⼔⌒㏜䉕⌕↪䈚䈩䈒䈣䈘䈇䇯
䊶⹜⮎䉕⺞⵾䈜䉎㓙䈮䈲䇮ᔅ䈝ቶ᷷䈮ᚯ䈚䈩䈎䉌↉䉕㐿ኽ䈚䈩䈒䈣䈘䈇䇯
䊶䊑䊨䉾䊁䉞䊮䉫⤑䈲ᚻⴼ⌕↪䉁䈢䈲䊏䊮䉶䉾䊃䉕↪䈇䈩ข䉍ᛒ䈇䈒䈣䈘䈇䇯
䊶⤑䉕䊤䉾䊒䊐䉞䊦䊛䈪൮䉃㓙䇮⤑䈫䊤䉾䊒䊐䉞䊦䊛䈱㑆䈮᳇ᵃ䈏౉䉌䈭䈇䉋䈉䇮䉁䈢䊤䉾䊒䊐䉞䊦䊛䈱᛬䉏䉇
㩷㩷䉲䊪䈏⴫㕙䈮಴᧪䈭䈇᭽䈮ᵈᗧ䈚䈩䈒䈣䈘䈇䇯
ଔᩰ⴫
⵾ຠฬ
Chemi-Lumi One
ⷙᩰ
⾂ᴺ
⵾ຠ⇟ภ
ኈ㊂
ଔᩰ
SP
಄⬿
05027-20
1 kit
(4,000 cm2)
25,000
Blocking
One One
Chemi-Lumi
䊑䊨䉾䊁䉞䊮䉫ᴺ䉇ELISAᴺ䈭䈬䈱᛫૕䉕↪䈇䈢఺∉ቇ⊛ᬌ಴ᴺ䈮䈍䈇䈩䇮⋡⊛䉺䊮䊌䉪⾰એᄖ䈱䉺䊮䊌䉪⾰╬䈱㕖․⇣⊛
䈭ๆ⌕䉕ᛥ೙䈜䉎䈢䉄䈮䇮䊑䊨䉾䉨䊮䉫ᠲ૞䈲ᔅⷐਇนᰳ䈪䈅䉍䉁䈜䇯ᧄ⵾ຠ䈲㜞ಽሶൻว‛䊶䉡䉲↱᧪䉺䊮䊌䉪⾰䉕ේᢱ
䈫䈚䈢䊑䊨䉾䉨䊮䉫ṁᶧ䈪䈅䉍䇮ᓥ᧪䈱䊑䊨䉾䉨䊮䉫ṁᶧ䈫Ყ䈼䇮ఝ䉏䈢ലᨐ䉕⊒ើ䈚䉁䈜䇯 䉁䈢䇮䊕䊦䉥䉨䉲䉻䊷䉷(POD)
䉇䉝䊦䉦䊥䊖䉴䊐䉜䉺䊷䉷(ALP)䈱㉂⚛ᵴᕈ䈮ᓇ㗀䉕ਈ䈋䈭䈇㒐⣣೷䉕૶↪䈚䈩䈇䉎䈢䉄䇮㐿ኽᓟ䉅಄⬿䈮䈩଻ሽ䈜䉎䈖䈫䈏
น⢻䈪䈜䇯
․˴㐳
٨ἨἿἕỿὅἂỆᙲẴỦ଺᧓ử‫ࠢٻ‬Ệჺ጑
٨᭗Ў‫҄܇‬ӳཋὉỸἉဌஹἑὅἣἁឋửҾ૰ểẲẺૼẲẟἨἿἕỿὅἂ๋෩
٨PODẆALPߩᣞእ෇ࣱỆࢨ᪪ửɨảễẟ᧸Ᏽдỉ̅ဇỂẆ᧏‫ࢸݥ‬ờϬᔺ̬‫܍‬ӧᏡ
૶↪଀
䊑䊨䉾䉨䊮䉫䈱෻ᔕᤨ㑆Ყセ
ᠲ૞᧦ઙ
䋨㩼䋩
᛫૕࿕ቯ䊒䊧䊷䊃䈱૞ᚑ
㪈㪇㪇
㪏㪇
㪍㪇
Blocking one
ᒰ␠ᓥ᧪ຠ
A␠⵾ຠ
㪋㪇
㪉㪇
㪇
㸣㩷㩷䊟䉩᛫䊍䊃䉝䊦䊑䊚䊮᛫૕㩷㩿㫆㫍㪼㫉㫅㫀㪾㪿㫋㪀
ἨἿἕỿὅἂ
㸣㩷㩷ᧄᶧ5୚Ꮧ㉼ᶧ㩷㩿⴫ਛ䈱෻ᔕᤨ㑆㪀
ੑᰴ෻ᔕ
㪇
㪌㪇
㸣㩷㩷PODᮡ⼂᛫䊍䊃䉝䊦䊑䊚䊮᛫૕ (1hr෻ᔕ)
෻ᔕᤨ㑆䋨ಽ䋩
⊒⦡෻ᔕ
਄䈱䉫䊤䊐䈲䊙䉟䉪䊨䊒䊧䊷䊃䈮䈍䈔䉎䊑䊨䉾䉨䊮䉫䈱෻
ᔕᤨ㑆䈫䊑䊨䉾䉨䊮䉫䈱ലᨐ䈱㑐ଥ䉕⴫䈚䈢䉫䊤䊐䈪䈜䇯
ᒰ␠ᓥ᧪ຠ䋨TBS♽䋩ਗ䈶䈮䌁␠⵾ຠ䈫Ყセ䈚䈩⍴ᤨ㑆
䈪䊑䊨䉾䉨䊮䉫䈏ቢੌ䈚䉁䈜䇯
㸣㩷㩷OPD䈮䉋䉍⊒⦡ (20min෻ᔕ)
෻ᔕ஗ᱛ
৻ᰴ෻ᔕ㩿䊍䊃䉝䊦䊑䊚䊮෻ᔕ㪀䉕ⴕ䈦䈩䈇䈭䈇
䈢䉄䇮⊒⦡䈚䈢䉌㕖․⇣ๆ⌕䈏⿠䈖䈦䈩䈇䉎
䈖䈫䉕␜䈜䇯
૶↪਄ߩᵈᗧ
䉝䊎䉳䊮䊶䊎䉥䉼䊮෻ᔕ䉕೑↪䈚䈢ᬌ಴♽䈲䇮ᧄᶧ䈱䉡䉲↱᧪䉺䊮䊌䉪⾰䈫੤Ꮕ෻ᔕ䈜䉎น⢻ᕈ䈏䈅䉍䉁䈜䇯䉁䈢ᧄᶧ䈮฽᦭
䈘䉏䈩䈇䉎䉺䊮䊌䉪⾰䈲䇮♖⵾䉺䊮䊌䉪⾰䉕૶↪䈚䈩䈇䉁䈜䈏䇮᷹ቯ♽䈱᛫૕䉇㉂⚛ᮡ⼂᛫૕䈭䈬䈫੤Ꮕ෻ᔕ䈜䉎น⢻ᕈ䈏
䈅䉍䉁䈜䇯૶↪䈮వ┙䈦䈩䇮੤Ꮕ෻ᔕ䈱᦭ή䉕䈗⏕⹺䈒䈣䈘䈇䇯
ଔᩰ⴫
⵾ຠฬ
Blocking One
ⷙᩰ
⾂ᴺ
⵾ຠ⇟ภ
ኈ㊂
ଔᩰ
SP
಄⬿
03953-95
500 ml
6,000
ߏᵈᗧ˴⹜㛎࡮⎇ⓥ↪એᄖߦߪߏ૶↪ߒߥ޿ߢਅߐ޿‫ޕ‬
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‫⽼ع‬ᄁขᛒᐫ㩷
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