FBC Lett., No. 51 (2016)
2016
6
2
旅と研究
野水 基義
東京薬科大学薬学部
4
4 糖脂質関連分子の機能および構造解析
∼蛍光顕微鏡と質量分析装置を効果的に利用して∼
樺山 一哉
大阪大学大学院理学研究科
9 DNA はどこまで（化学的に）入れ換え可能か？
名古屋大学大学院工学研究科
樫田
啓
15
九州大学大学院工学研究院
大阪大学大学院工学研究科
特任助教
与那嶺 雄介
博士前期課程２年
高
靖馳
22
イリノイ大学留学体験記
鳥取大学大学院工学系研究科
稲葉
央
26
第 19 回 生命化学研究会、第 10 回 バイオ関連化学シンポジウム、第 43 回 国際核酸
化学シンポジウム（ISNAC 2016）、第 26 回 バイオ・高分子シンポジウム
受賞、異動
編集後記
4
4
I
8
9
4
I
8
6
4
I
1
I
I
I
I
H
4
I
4
I
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4
9
4
4
4
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6
6
I
9
I
I
4
I
4
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4
4
9
6
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I
4
I9
9
1
4
I
4
4
I
I
4
4
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4
I
69
2016
-3-
5
No. 51 (2016 July)
(
(
)
[email protected]
1.
1996
20
N-
GM3
(1)
GM3
(2)
GM3
GM3
GM3
IR
IR
IR
(3)
GM3
IR
GM3 IR
(4, 5)
(6, 7)
-4-
No. 51 (2016 July)
2.
(8)
Fluorescence
recovery
after
photobleaching
FRAP
FRAP
(ROI)
(
)
1
GM3
IR
caveolin-1
GM3
FRAP
GM3
IR
GM3
IR/ caveolin-1
(4)
-5-
2
No. 51 (2016 July)
FRAP
Y
2
2
FITC
20
(
3A)
Rhodamine-C18
2.0 µl/min
5.0 µl/min
(
3B)
EGFP
CHO-K1
(
4A)
(IR-EGFP)
β-
MβCD
FRAP
FRAP
(
4B)
IR-EGFP
”
-6-
MβCD
(
4C)
No. 51 (2016 July)
3.
2
GM3
GM3
DRM
GM3
TOF-MS
DRM
Triton X-100
MS
1,2-
DCE
(
)
99.99%
MS
(6)
TNF-α
3T3-L1
DRM
DCE
Triton X-100
DRM
LC-MS
MS
Svennerholm
LC-MS
GM3
100 pM 96
TNF-α
2
5
3T3-L1
TNF-α
GM3
2
C57BL/6J
KK
KKAy
C16
C18
(9)
ob/ob
C16
C24
SM
SM
(10)
GM3
LC-MS
2
4.
-7-
No. 51 (2016 July)
LC-MS
5.
1.
Kabayama K, Ito N, Honke K, Igarashi Y, & Inokuchi J (2001) Suppression of integrin expression
and tumorigenicity by sulfation of lactosylceramide in 3LL Lewis lung carcinoma cells. J Biol Chem
276(29):26777-26783.
2.
Tagami S, et al. (2002) Ganglioside GM3 participates in the pathological conditions of insulin
resistance. J Biol Chem 277(5):3085-3092.
3.
Kabayama K, et al. (2005) TNF-α-induced insulin resistance in adipocytes as a membrane
microdomain disorder: involvement of ganglioside GM3. Glycobiology 15(1):21-29.
4.
Kabayama K, et al. (2007) Dissociation of the insulin receptor and caveolin-1 complex by
ganglioside GM3 in the state of insulin resistance. Proc Natl Acad Sci USA 104(34):13678-13683.
5.
Sekimoto J, Kabayama K, Gohara K, & Inokuchi J (2012) Dissociation of the insulin receptor from
caveolae during TNFalpha-induced insulin resistance and its recovery by D-PDMP. FEBS Lett
586(2):191-195.
6.
Suzuki Y & Kabayama K (2012) Convenient and rapid removal of detergent from glycolipids in
detergent-resistant membrane microdomains. J Lipid Res 53(3):599-608.
7.
Kojima H, Suzuki Y, Ito M, & Kabayama K (2015) Structural characterization of neutral
glycosphingolipids from 3T3-L1 Adipocytes. Lipids 50(9):913-917.
8.
Kabayama K (2012) Modulation of growth factor receptors in membrane microdomains. Yakugaku
Zasshi 132(4):417-423.
9.
Tanabe A, et al. (2009) Obesity causes a shift in metabolic flow of gangliosides in adipose tissues.
Biochem Biophys Res Commun 379(2):547-552.
10.
Samad F, Hester KD, Yang G, Hannun YA, & Bielawski J (2006) Altered adipose and plasma
sphingolipid metabolism in obesity: a potential mechanism for cardiovascular and metabolic risk.
Diabetes 55(9):2579-2587.
-8-
No. 51 (2016 July)
[email protected]
DNA
DNA
A
T
G
C
DNA
DNA RNA
DNA Origami
Romesberg
[1]
2014
D-ribose
[2]
D-(deoxy)ribose
D-threoninol
D-threoninol
D-ribose
[3]
[3b]
NMR
-9-
No. 51 (2016 July)
DNA
M
DNA
[4]
Tyagi
Molecular Beacon
MB
[5]
MB
In Stem Molecular Beacon
(ISMB)
MB
DNA RNA
[4a]
PLL-g-Dex
ISMB
- 10 -
DNA
No. 51 (2016 July)
ISMB
[4c]
500
Cy3
[4b, 4e]
ISMB
[4e]
RNA
[6]
DNA
p-
4A
[6a]
DNA
4B
G-C
p[2+2]
(A)
(B)
(C)
(B)
Z
340nm
4C
[6c]
[7]
[8]
5A
π
DNA
NMR
- 11 -
No. 51 (2016 July)
(A)
(B)
(C)
5B
[8b]
5C
[8a]
[8c, 8d]
[9]
D-threoninol
P
P-Q
Q
DNA
DNA
- 12 -
P-P Q-Q
No. 51 (2016 July)
DNA
DNA RNA
- 13 -
No. 51 (2016 July)
[1]
a) I. Hirao, Curr. Opin. Chem. Biol. 2006, 10, 622-627; b) D. A. Malyshev, K. Dhami, T. Lavergne, T. Chen,
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- 14 -
No. 51 (2016 July)
ImPACT
[email protected]
2010
2016
ImPACT
nm
DNA
D
MEMS
µm
MOF
bottom-up
top-down
4
Programmable motion of DNA origami mechanisms
A. E. Marras, L. Zhou, H.-J. Su, and C. E. Castro, Proc. Natl. Acad. Sci. USA, 2015, 112, 713–718.
DNA
100 nm
DNA
2
3
DNA
DNA
1a
1b
TEM
11-147
79.7°
29.2 nm
2
1c
TEM
1 DNA
(c)
TEM
-
(d, e) Bennett
- 15 -
(a)
(b)
1
No. 51 (2016 July)
3
Bennett
1d, e
DNA
Programmed switching of single polymer conformation on DNA origami
A. Krissanaprasit, M. Madsen, J. B. Knudsen, D. Gudnason, W. Surareungchai, V. Birkedal, and K. V.
Gothelf, ACS Nano 2016, 10, 2243−2250.
DNA
DNA
DNA
2
APPV
9
DNA-APPV
2a
DNA
DNA
DNA
2b
AFM
DNA
2b
2b
DNA
RgDNA
2c
LgDNA;
2d
2 (a) DNA
DNA
(e)
DNA
(b)
(c)
DNA R DNA
(d)
LrDNA
2e
2
DNA
FRET
6
3D printed microtransporters: Compound micromachines for spatiotemporally controlled delivery of
therapeutic agents
T.-Y. Huang, M. S. Sakar, A. Mao, A. J. Petruska, F. Qiu, X.-B. Chen, S. Kennedy, D. Mooney, and B. J.
- 16 -
No. 51 (2016 July)
Nelson; Adv. Mater. 2015, 27, 6644–6650.
µm
direct laser
writing
(
3 (a)
)
(b)
(
)
(c)
(d)
3a
3b
3c
3d
Cellular cargo delivery: Toward assisted fertilization by sperm-carrying micromotors
M. Medina-Sańchez, L. Schwarz, A. K.Meyer, F. Hebenstreit, and O. G. Schmidt; Nano Lett. 2016, 16,
555−561.
4a
Adv. Mater.
2015
4 (a)
(b) (i)
(c)
(i)
(iii)
70 µm/s
HOS
4bi
4ci
4cii
4ciii
- 17 -
(ii)
(ii)
No. 51 (2016 July)
Top-down
DNA
- 18 -
No. 51 (2016 July)
Gao Jingchi
2
[email protected]
2
Multiplexed labeling of genomic loci with dCas9 and engineered sgRNAs using CRISPRainbow
Ma, H.; Tu, L. C.; Naseri, A.; Huisman, M.; Zhang. S.; Grunwald, D.; Pederson, T. Nat Biotechnol. 2016, 34,
528−530.
CRISPR/Cas9
CRISPR/Cas9
Cas9
dCas9
RNA Single-guide RNA, sgRNA
dCas9
sgRNA
dCas9
CRISPR/Cas9
CRISPRainbow
dCas9
1. CRISPRainbow
sgRNA dCas9
sgRNA
- 19 -
No. 51 (2016 July)
sgRNA/dCas9
sgRNA
MS2 PP7 boxB
MCP
N22
BFP
RFP
PP7
PCP
1
1
GFP
1
sgRNA
MS2
boxB
sgRNA
7
CRISPRainbow
15
CRISPRainbow
sgRNA
dCas9
sgRNA U2OS
2
CRISPRainbow
dCas9
2. CRISPRainbow
Visualization of protein−specific glycosylation inside living cells
Doll, F.; Buntz, A.; Späte A. K.; Schart, V. F.; Timper, A.; Schrimpf, W.; Hauck, C. R.; Zumbusch, A.;
Wittmann, V. Angew. Chem. Int. Ed. 2016, 55, 2262−2266.
Glycosylation
N
O-GlcNAc
Protein of interest, POI
3
EGFP
Ac4GlcNCyoc
Ac4GlcNCyoc
Inverse-electron-demand Diels-Alder
reaction, DAinv
FRET
EGFP
EGFP
EGFP
- 20 -
No. 51 (2016 July)
FRET
Fluorescence lifetime
imaging microscopy, FLIM
FLIM
FRET
Ac4GlcNCyoc
EGFP
Ac4GlcNAc
FRET
EGFP
3.
Foxo1 p53 Akt1
4
O-GlcNAc
EGFP
HEK293T
Ac4GlcNCyoc
TAMRA-Tz
FLIM-FRET
FRET
Ac4GlcNAc
EGFP
FRET
Akt1
Akt1
O-GlcNAc
- 21 -
4.
No. 51 (2016 July)
[email protected]
2015
1
2016
2016
UC
2
3
Christopher J. Chang
Chris
Jefferson Chan
Jeff
Jeff
iCeMS
Jeff
University of Illinois at Urbana–Champaign, UIUC
−20
UIUC
Prof. Lu
Prof. Moore
- 22 -
No. 51 (2016 July)
NMR
UIUC
Jeff
Jeff
Jeff
Jeff
Jeff
Jeff
- 23 -
No. 51 (2016 July)
Jeff
individual meeting
Jeff
Genetically-encoded
sensors
Jeff
“proposal competition”
iCeMS
Jeff
Jeff
Lab Olympic
8
2014
SciFinder Future Leaders in Chemistry
https://www.cas.org/products/scifinder/futureleaders-alumni
CAS Chemical Abstract Service
SciFinder
CAS
Mt. Hood
- 24 -
No. 51 (2016 July)
2014
“
”
Mt. Hood
Jeff
Jeff
- 25 -
No. 51 (2016 July)
第 19 回
生命化学研究会
∼生命化学・温故知新∼
https://res.tagen.tohoku.ac.jp/FBC/
2016
8
1
13:30 ~ 2
12:00
(https://www.kgh.ne.jp/10/access/)
60
90
1. JR
2.
)
JAL 291
0800-
ANA 3811
0720-
0935,
0900
3
0845-
1038
545-
731(
)
541
0943-
1020
0804-
0943-
8,000
18,360
E-mail
tsumu @ b.s.osakafu-u.ac.jp @
)
1043
6
20
)
40
6
20
A4
b.s.osakafu-u.ac.jp
tsumu @
)
)
(https://res.tagen.tohoku.ac.jp/FBC/)
)
@
(
599-8531
TEL
1-1
072-254-9834
- 26 -
___
__
No. 51 (2016 July)
第 10 回 バイオ関連化学シンポジウム
（第 31 回 生体機能関連化学シンポジウム、第 19 回 バイオテクノロジー部会シンポジウム）
http://jointsympo.csj.jp/
9
7
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JR
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http://jointsympo.csj.jp/
1-1
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/FAX (0761)51-1650
E-mail: [email protected]
http://jointsympo.csj.jp/
- 27 -
No. 51 (2016 July)
第 43 回国際核酸化学シンポジウム
The 43rd International Symposium on Nucleic Acid Chemistry 2016, ISNAC2016
http://isnac2016.org/
2016
9
27
29
5
860-0047
1. Chemistry of Nucleosides, Nucleotides, and Their Analogues
2. Medicinal Chemistry of Nucleosides and Oligonucleotides
3. DNA/RNA Chemistry and Biochemistry
4. DNA/RNA Structure and Recognition
5. Ribozymes, siRNAs, and miRNAs
6. DNA/RNA Materials and Diagnostics
7. Drug Delivery Systems and Nanotechnology of Oligonucleotides
2016
4
11
6
17
2016
4
11
7
8
2016
4
11
8
12
30,000
10,000
35,000
15,000
http://isnac2016.org/
E-mail: [email protected]
Tel: 096-342-3873, Fax: 096-342-3679
- 28 -
1-14-1
No. 51 (2016 July)
第 26 回 バイオ・高分子シンポジウム
http://www.spsj.or.jp/entry/
7
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)
29
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- 29 -
No. 51 (2016 July)
27
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27
2016
3
2016
3
2016
4
2016
4
2016
5
33
28
28
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2016
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1
E-mail: [email protected]
- 30 -
No. 51 (2016 July)
4
14
16
5
9
6
No. 51
No. 52
2016
28
6
10
23
[email protected]
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