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Key words: Brachyspira pilosicoli, bacteremia, intestinal spirochaetosis, blood culture,
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Hampson, D. J., S. L. Oxberry, T. La. 2006. Potential for zoonotic transmission of Brachyspira
pilosicoli. Emerg. Infect. Dis. 12: 869ῌ870
<=> 1999.
KqrGVg U?@
>Ag*] D 45: 201ῌ206.
Mikosza, A. S., D. J. Hampson. 2001. Human intestinal spirochetosis: Brachyspira aalborgi
and/or Brachyspira pilosicoli? Anim. Health
Res. Rev. 2: 101ῌ110.
BC, D , EF 2008. GH 48 ."/001½« Vol. 20
No. 1
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16)
17)
¡¢IJ£¤¥g@¦W
Kq
rGVg K:*/ 26: 836ῌ840.
Smith, J. L. 2005. Colonic spirochetosis in animals and humans. J. Food Protec. 68: 1525ῌ
1532.
Hovind-Hougen, K., A. Birch-Andersen, R. Henrik-Nielsen, et al. 1982. Intestinal spirochetosis:
Morphological characterization and cultivation of the spirochete Brachyspira aalborgi geb.
nov., sp. nov. J. Clin. Microbiol. 16: 1127ῌ1136.
Mikosza, A. S. J., T. La, C. J. Brooke, et al. 1999.
PCR amplification from fixed tissue indicates
frequent involvement of Brachyspira aalborgi
in human intestinal spirochetosis. J. Clin. Microbiol. 37: 2093ῌ2098.
Tanahasi, J., T. Daa, A. Gamachiet, et al. 2008.
Human intestinal spirochetosis in Japan: Its
incidence, clinicopathologic features, and
genotypic indentification. Mod. Pathol. 21:
76ῌ84.
Bait-Merabet, L., T. Arnaud, L. Patrick, et al.
2008. Brachyspira pilosicoli bloodstream infections: Case report and review of the literature.
Ann. Clin. Microbiol. Antimicrob. 25: 7ῌ19.
Trott, D. J., N. S. Jensen, I. S. Girons, et al. 1997.
Identification and characterization of Serpulina
pilosicoli isolates recovered from the blood of
critically ill patients. J.Clin. Microbiol. 35: 482ῌ
485.
8BLM ONO PQ§O¨ 2005 D
G©@
¥* Brachyspira pilosicoli KqrGVgeªR]% 1 ./0«
15: 187ῌ196.
Brooke, C. J., K. Rini Margawani, A. K. Pearson,
et al. 2000. Evaluation of blood culture systems
for detection of the intestinal spirochaete
Brachyspira (Serpulina) pilosicoli in human
blood. J. Med. Microbiol. 49: 1031ῌ1036.
S¬, ®¯° 2007. Helicobacter cinaedi
>Ag >A ¥g T± 37: 336²339
UVW X:§ ³DY´ 1997. Serpulina Zµ¶·[\] ."¸ 124
¹ºb 124: 230.
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Brachyspira pilosicoli isolated from pigs in
Japan. J. Vet. Med. Sci. 66: 875ῌ877.
Kraaz, W., B. Pettersson, U. Thunberg, et al.
2000. Brachyspira aalborgi infection diagnosed
by culture and 16S ribosomal DNA sequencing
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vitro antimicrobial susceptibility of Brachyspira pilosicoli isolates from humans. Antimicrob. Agents Chemother. 47: 2354ῌ2357.
Brachyspira pilosicoli 49
A Case of Bacteremia Caused by Brachyspira pilosicoli
Sumie Chida,1) Hitoshi Asukabe,1) Kiyofumi Ohkusu,2) Takayuki Ezaki2)
1)
2)
Department of Clinical Laboratory, Tokoname Municipal Hospital
Department of Microbiology, Gifu University Graduate School of Medicine
Brachyspira pilosicoli is the etiologic agent of human and animal intestinal spirochetosis and is rarely
implicated as a cause of bacteremia. Here, we describe the case of a B. pilosicoli spirochetermia in a 56year-old male patient su#ering from cardiogenic shock. Blood cultures were positive after 10 days of
incubation. After 4 days, the isolate was subcultured on 5 sheep blood-containing agar plates under
anaerobic conditions at 35 and the growth appeared as a thin film with weak b-hemolysis. Furthermore,
the isolate was identified as B. pilosicoli by means of nucleotide sequencing analysis of bacterial 16S rRNA
gene. This case would be di$cult to be observed unless the extension of incubation time for blood culture.
Vol. 20
No. 1
2010. 49

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