Genome Network ProieCLEntry clone
RIKEN c:one ID:M01C033F08
Vectori pDONR221
G
Accession
● Plasmid DNA purification
Date : 100929
Culture:LB(25 ug/ml Kanamycin) 3 ml → 37° C
Date : 100930
Purification:QIAGEN Miniprep kit→
O/N
dH20 100 ul
● Digestion by restriction enzyme/ConcentrariOn calibration
Date i 100930
DNA concentration(OD.):
3512ワ
DNA
ng/ul
l
Restriction enzyme(Hinc Ⅱ+EcoRV)
ul
05+O.5 ul
Buffer H
l ul
dH20
7 ul
Total
10 ul
Erectrophoresis:
l%agarose gel,l X TAE Buffer
Marker:2-Log DNA Ladder(NEB#N3200L)
kb
)2
たと
〈Expected digestion pattern fronl BC029264.l CDS〉
2263.1205 bD
● Attust plasmid DNA solujon to 25 ng/u: ∼preparajon for shipping∼
Shipped:25 ng/ul,40 ul
Final concentration:25 ng/ul
丁otal
脚
融
］
﹁
Date : =恭01
Operator:3.101001.furu
PrOieCt:GNP
Sample:M01C033F08_M13.C04_101001100K
Resu:t:M01C033F08_M13.C04_101001100K
:nstrumentl CEQ System(Ver・ 9,0.25)
Ana:yzed
Anaiyzed Data
￨
￨￨
古‖ll
Analyzed Data
。相 ︱
６
Analyzed Data
︲
︲
︱
︲
Mon 10ノ 04r1011:lo:36
Pagel l
Operator:31101001.furu
Prolect:GNP
Sample:M01C033F08二 T7.D04_101001100K
Result :M01C033F08二 T7.D04_101001100K
instrumenti CEQ System(Ver・
9・
0.25)
Analyzed Data
︱
︱＝︱
︱︱
ｏ知 ︱︱
”“ ．
3 000TTTOTATC
OAAAAAATo TTTOC^AOTTAAAATCC O T A C TACA TCT■
TCAATCCATOCCTTAT●
。
４
２ 一
誹
。
“¨が２
．“ ¨ ¨ ¨
Analyzed Data
Ａ
Ａ
Ｉ
Ｉ
TAC31CAATTCTO・
CAOCCA●
01
Ｉ
Ｉ
Ｉ
Ｉ
Ｉ
Ｈ
﹁
︱
︲
︱
︲
。︹ ︱
６
Ｔ
。
４ Ｔ
TCTAA● TAAAA●
日
︱
︱
︱
︲
山
５
。
、¨ “ ¨ ¨ ¨
“３
．２
Mon 10′ 04r10 111lo:39
Analyzed IData
Page:1
2010/10/()1 16:47:36
Toxt3
1 GEllEΓ Yヽ
Date 1 2(ヽ
: Nuc]eotide Seqt,once Homology
り1lit Sizc tc)(Eompa re
10 10 01
ri(,k up llocaし
lst IIり ,lootido Sequr‐
io口 ll●
‐
1
nce
: Bて ,029264
Fi le lNlanle
Sequ・
・ rice size
2010/10/01 16:4フ :36
Text 3
い●la l
1 1Tnt】
: 918
2nd Nu● lootide Sequence
i 140」 (]033r08 i113.C01 101001100K fasta
, 7:〕 6
Fi le llame
sequen(e s,to
Unlに Siス o し()Cc nlpa re ‐ ら
PJck ttp lェ )catl()rl ll、 ) ・ , 1
1 607 / 61l bp]
218'
20H
1346/
、
llJT/011T :,core :
ATGAC CTTC〔 :11:AGA Al｀ 1ハ (,CCCll「 !
C(lA(｀
2400 '
l」
ぜ
GGCT―
GCCC TCArCGAハ cc T― r.A(]GCTrlT
8' C(,CATC「 rCTc
121・ T(:ACCA■ GAG CTTCCl:1'ACA AT],AC(,C(｀ T:: C(lACCCAT,ゞ 3 Tl'C´ r(:TTTCA TCTCGACT「 T
′ GA
ψ
ヽC(:Gハ AAA GCCAT]・
じ
ATCTlⅢ l(,TA :,TACTTメヽ
,73' TI:ACC()1'TAC ´
`:CT TTC(lTCA3TC A′
181・ T(3Ar.G(,rTAc TArCir■ ￢cTA CTACTT,島 CT TrCclて :ACrC AS_CGr3AAAA G(lCATTTG`3A
135・
tl・
GAAATCAGAr CACATCGGA■
1・ IACGA`]´ JOA
21lm CAGCTCA(lCT Gβ コヽTATAユ (]A TTTCAC(〕 rTG GF、■ATGA(lAl' CACATCCCAA TTハ
3,3' GTGGAGTGl r(lCCczvヽ
.rCAT TCT(｀
C rl)TcG A螺I(lG
453'
∼
Gr.A`]Aじ
A
]G
rC]｀ C 'r(」
`1'G16(;TC CCA3TTCl‐
ATCAl l]CTCI⊂
301" GTGGハGT(,TT (lCGC′ ヽ
Tて ,TC(:
A`ヽ へGG(lrsTc
ゝ
A TcAGA」 へ7GCC 'GT(｀ AT()CTT TCACCrご
GG′rTCGノ 、
Tr」
T(:Gr('lCGTc (lCACTTCCTG
1l cノ 、 cACGCCC
(ぢ
AAACACC(:Aて
(・ C― G`)ACCA
i」
CAC lAAIヽ GT'Al,TT
Ti′ ヽ
/・
`]GCTCTACTTT TACAC2ヽ
4,1" ACTPAiCCC'r(,GCc(,T(AT(, G■ へ CGTGGAG CClGTACT17. .I.ACACATハ
698' ATCCAAAl・
ll
CGC
TA´ rA GTCCCCiGT(,A TCA「 rAA(:cAコ ヘ (,TGA3′ ヽ
(lCA(:G(16AC GGλ A理 ヽ
(〕
rr iぜ
GcAcTC(じ AT TCAGAル ヽGGA GTCTAl・ CrGA
CAGTCATCAT lCAACrGGGG
195" ATCCAAArTT l'暉 cACTCCAT T(｀ Aけ AAAGOヽ GTCTATt3■ (lA CAGTCATC.AT TCハ
758' AGハ し
ヽ:PCCACA TCC′ ヽ
Cr(,GGT CAG(,CAT rAl‐ ATCAl'C・ PATC lATTTA、
,C・「T
ヽG::C(1(:G
コ
CCT【 3CCT(lCC
255" A(フ tヽ T6CA(A TCC:AGTljGGr CACccATrAC ATCAlG′ [ATC 「:ATTT7ヽ C IPl (,CrGCc・ :Gcc
818' AIハ ATTCCCl' ACTTTTACTT ACA′「 T`CC:,'C TCGA(〕
ACl・
rA CACC`FA3Al‐ T GCt'(7:ム TTCT
TT(]JI
315" ACAATTC,CCl' F《 〕TTTlム CTr ACAl'TCC(:]て , lG(:ACACTTA CACCTAGA`:T C(ICCAコ ヽ
,
ヽ
CA13GCAG
ヽrGcc .rGTcATCCTT T(,A('CAAlΓ l T CAGCAGG(CC )♂ノ
361'' ムAGら AI「 G`ハ A rcACハし
5131 ACTCAl'(:`CT GOCCCTCATC G2ヽ へCCIビ GG′
AT― ■♂Lへ TCAA
ACCA(]GGGAC Gt7AAAATArA GTcccGGTGA rCAlAA(1(,AA CCr`ン vAr.Gc
(,C(ATG′「C`rc
rl‐
.´
・1・ ぜ
■
ACAC
,5' cAGハ みGGGAC CAtTA,Gl 6● T GATCA:じ CTAC AAC・ [ATCAAC CTACC(:(lCA6 1′ V■ GTATて
CAlAG lI「 C7(:TTJ.CA TCTCCACT'rT
6,〕
333' CA(:Cl CAC,(,■ ' CAATATAACA J｀ ■rCAGGT・ IC
A(,―
′
ヽ
5'8: 6ACノ ヽ´ゴ
3C(,AG C].み し
「 GGGCl G′ rrCATCTAC AACT2YrCAAG (:TA(:(lG(〕
♂」
PCAAC(,1,GG
AA NrCS、 cGT`G
8'3' PTCACCAGGA CGC● さ]、CTCA A■ 1ハ カもへGACA GATCTら ハンヽGA
37も ‖rり ]cAc(,AC(,A
CGCCAAGTCA AAT聟 ヽ八
`:ACA GATGTて
938' CTCC(]AClTC TCAAACAACG GGA「
lハ
AACCTATT(:A C(,ACCtt TC´
′
ヽGA AA(」
(1■
ふ
'ATT(:A CCAGCT`rCAハ
GA()GAA TrAGAC(TAA ATGAACACAA ()・ [GTGTTCTT
′
、 ACAA C16T(ヽ TTGI『「
435'l CTGC`IACTTC rcAAA(1ハ ハSG GGArsAC(」 小′ヽriFAG2■ 3(:FAA ″r(:う し
(〕
573' CAt,CA(:'ヽ ま
3メVヽ
(〕
ヽ
コ
ヽ
(3C′ ヽ
′
T
GGアヽ
G
A(1(]TACG GGCAGTA´ ′ヽ
GC(,Gl GATヽ :A TCTACハ ムCTA l'C′ ヽ
￢
ハA C(:G]て ]ヽ ,■■AT(]tら
481・ CA(,CAGハ ま
GAl.CA TCTAヽ
C(」 ■
]A]ヽ
CTA rCAAGGTACC CGCA(11ハ
ル
ヽ
AC
∼
5し
6931 AACら (,ATCt'A′ やヽTTTJ]ら CAC, TCGATTCA(3A ttACt,AGTCrA TGTGACAGTC
753' TGGC(:A■ ヽAT (:CAC ATCCム
G rG(」
バ
「 CATTCAAC
AAGGAGTCTA rGTGACACTC ArCA,TGAAG
GTcACCC ArTACATCAT ,TA.rCTAlilT A¨ CCl TC(lTC
661・ 「Pl‐ G(`GA(:AAT C(:ACATCCAC r(,GGF(:ACCC ATAA(,llTCAT (:TATCiFAl・ Tr A(,(lcTT(lCTG
812' GCJIG―
tて
｀
,ACA ATlCCC■ AC] TTTハI:rTAGA TICCGl(IICC AG'“ 1]TACAC Cl｀ ACACr(3CC
72," GC■ てiC(lt]ACA AT(3CCC
lst NucleoLi(le Sequerice
F」 le Namo
sequerlce Size
2:、
: Bl_()29264 1 tlr〕
d Nucleく )に ld● SOqlュ e
Fi le Nante
sequence SLこ
rlco
: M01C033『
●
t,
: ,18
1)3 T′
[)01 101001100K fasし
: 734
1
a (Cofrpl erno rita ry)
GTACGCバ rl'「 TAArTl'GcAA ACATTrT7・ rc
1058' CAT卜ヽ GG(〕 AT GCATTGACCC CT(,CCl'TTTA CCCCATAG`,A CATCI:CCCAP
TAFTTCCCAIF GrcTcAcCAC Gむ GAC(lGAA● A.rATASTCCC i'G:「 GttrCATA A(lCAACCTGA
601" Aし ヽ(,GCハ TCGA ]ム Tl・「 TCCAC l'CG]」 ●rcACA
ll C CCざゝ、
GAllGTA 〔
)1'iCGCArl・ r TAハ CirT(lcnA AcATTTTT・ rC
49=・ l GCTTTCACA (lAl AC,コ ヽACC CCAAriAl CTF・
133' TATTTC('CAr ttTC′ rcACCAC G(3r‐ ACGGん へ A.rATA(,T(,CC GGTCArGATA A(ICAACCTGA
541・
,98' TCCTTTCACA CATACハA
らTCCAAGlGT
〕
C('CAl' G`『 r.CAAG16.r
い!' CATAハ G(:CAT CCハ frTGAて ,c(, cTCCCll.rTTA G()cCA「 A('(;A CAT(〕 り
ll18'(lA(,ATCCT(:A
ハし
ヽACTT,ヽ A
C15" GA(:ATCCTGA AAA(,TCACCC ACC■ TTCTTG lヽヽAAACTTG CCAJ TA`1'AA●
ヽ
CCATT(lC
Aハコ