CTC(末梢血循環腫瘍細胞)を簡単にキャプチャ－
できる画期的なデバイス
画期
Filtrattion tank
Nozzle
e/Holder
Screen
nCell® MB-LC Filtration Unit
ScreenCell® は生きた細胞をキャプチャーすることが
できるのでMolecular Biology analysisが可能
a
b
Capsule/Filter
c
d
e
f
Microporous
Filter for
Live Cells
Tissue
Culture
Well
Capsule/Filter
Eppendorf®
Tube
Copyright ScreenCell ©
2
ScreenCell® は従来の細胞診的解析も可能
b
d
January 2012
Microporous
Filter for
Fixed Cells
ScreenCell® Cyto
c
ScreenC
Cell® CC
Filtra
ation tank
Microporous
Filter for
Live Cells
O ring
e
f
Rod
Circular
Coverslip
B
Blood
Waste
W
Protective
Membrane
Collecttion Tube
Noz
zzle/Holder
ScreenCelll® Cyto and
d CC Filtration Unit
a
Copyright ScreenCell ©
Tissue
culture
well
Microscopy
Glass Slide
3
ScreenCell はキャプチャーしたＣＴＣを様々な解析方法に供して
キャ チャ
を様 な解析方法 供
キャラクタリゼ－ションすることが可能です…
Next Generation sequencing
mRNA and protein multiplexes
PCR and non-PCR assays
FISH assays
LINKS
ICC
CTCs are
isolated on
removable
support
January 2012
Cell culture
Copyright ScreenCell ©
Platforms using: - Cartridges,
- Plates
- Slides 4
New applications of CTCs using
ScreenCell’s technology
Applications (b)
Objective
Genetic characterization
• DNA & RNA sequencing
i
(Sanger / next generation
sequencing)
• Non
N
PCR assays
(mRNA multiplexes)
• PCR assays
Identify gene alteration generating
the disease
•HER2 for breast cancer (a)
•EGFR
EGFR (a)
•Alk for lung cancer
•KRAS for colorectal cancer (a)
Protein multiplexes (a)
Phenotypic characterization (a)
Cell culture (xenoengraftment)
Functional characterization
Indicate the presence of tumor stem
cells among CTCs (a)
(a)
(b)
Data available
Copyright ScreenCell ©
5
On any standard platforms using Cartridges, Multi-well plates and Glass slides
特殊なフィルターにより
ＣＴＣを失いません！
Blood cells
HT29
FACS ANALYSIS
Filter
3 ML blood
+ 3000 GFP+
HT29 tumor cells
Collection of
cells on the
filter
Detection of GFP+ HT29
cells on the filter
Vacuum
Left over
ScreenCell device
FACS ANALYSIS
Blood cells
January 2012
Copyright ScreenCell ©
HT29
6
No GFP+ HT29 cells
detected on the left over
Braf sequence analysis of CTCs isolated by size from whole
blood of patients with Melanoma
Primary tumor
Braf V600E mutated
Patient 17
Isolated CTCs
Primary tumor
Braf WT
January 2012
CTCs of Patient 18
Braf V600E mutated
Whole Genome
Amplification
Nextt Generation Seq
quencing
g
Patient 18
I l t d CTM
Isolated
CTMs
Copyright ScreenCell ©
CTCs of Patient 17
Braf Wt
7
Xenoengraftment in immunocompromized mouse
Insertion of the filter under the mouse skin
Dissected tumor and filter in a Petri dish
Filter with O-ring
January 2012
Tumor in situ
Tumor developped
on both sides ofCopyright
ScreenCell filter
ScreenCell ©
Tumor
8