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DPA1 20
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A*02011
N Null
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‰ ď9Ù+F9€åä¯LÙ+
L Low Expression
A*020101
A*24020101
¼°L ¼6)/@/ƀåä¯9;ď
S Secreted
¼Č ¼ ¼č"yH¥4GJ4I
synonymous mutation
C Cytoplasma
mutation outside the coding region
A Aberrant
" ;+59 @52!KJ4
H LöI~èª"5#4#/.&
HLA Nomenclature 2002
5 :Á; L2!&6"æ
GJ4I€À9 :Á; "eš'J4I'G9&J@5­ž±—; 6 0%51/i†
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SSP:sequence specific primer
bB (@ £6‹– b
SBT:sequence-based typing
4 6” Î !
@ , $ 0\ x " 0 @ ! £06‹– b0;‚Í b0;
SSCP:single strand conformation polymorphism
v¹0@
RSCA: Reference Strand Mediated Conformation Analysis
ÖÓ
£ÚLCRYGB¬1'+w46
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HLA
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Ăþ ÅĆMY\…"B.A<4<@<,0<® 2³Å/A ˆ¨·°–2A
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ć A5 9/”Ÿ%A*;
DNA resolution 4ª±…#*
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SSOP L
Y
A 4 Å/5OoseÍÓÔemHhs
RFLP L
N
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SSP
L
N
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N
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Y/N
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RSCA H
N
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HLA
A¡4!02? RsNKrQs0VHbrO
Uc\IJG5§û/A
Ąþ ÅĆ
t·ô4Úy¿î“‘ð3|—#GNnoGi]PoùÄƄ4µ„ Ó
2A!0EƒÑ#*¾½Å/A® 2³Å/AƄ4É `YcFsÝ­PoË 213>@µ„ 5•…#Ó2AGno/<Œ$µ„ 32A!0AVHbrO3ÑA
*;35†2¸w¾ç0Gno´Õ4 E(CA§Aö?B*GnoEšì0#
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â#€@~#*`r]EÖ.ó0#<t ”Ÿ%B6 GnoÒ¹4Ð̤?B
A
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ąÿ²#¯å
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sdš¨#.A°†â4asTt·4Xksd3Nj"B.@!4u/ã}Àë#*
¾y E_HdnWHT"'A¾y ދ#*asT4âE›Ñ4cqsQH\jsVs
/º%B614eqsd0ˆ¨#*DAˆ¨®ú5eps\Å= Å0üx#.
@9*cqsQH\j\ns4Þ»?GnoEᄂ™%AUc\<Ñ«"B.A°
¾y?–ñ¾y9/¨Ñ‰à2³Å/A
āþ Å ŽĄ
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denaturation
oѫ"B.A!4
annealing
5³4 +
ã}Àë"B.A
E”Ÿ#*emHhs0Œ$ð
4emHhs/¾y E”Ÿ
#Š 04õ/fZq
[ksepYNSE¢­"'A
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RSCA
#ã}`r]4µ„ Eʙ%
(Reference Strand Mediated Conformation Analysis)
AƄÃ4µ„ 4æÁ4*;
×ÿ7tò¼ÄHʹ{&F-°z CJab!ãzx—
&FE QfNLdPfHÊE! 3Ë5D«YfN7Òç.$2Ã>-?¿ìШ
5­Â2E' 'Jab7Ú=G+6B/18|y2"5
Ćă 7‰þ
Jab8”¦6˜=_WfN5
A*0226 + A*0302 = A*0212 + A*030101
ów!š5%3 C H%5/A*0302 + A*0305 = A*030101 + A*0310
ambiguity
A*0307 + A*2601 = A*030101 + A*2612 locus
/allele
3'1@ĄÚng7Jab7Ú=G+
A*0309 + A*110101 = A*030101 + A*1106 A
179/274
H…—2"5%3!EŒ
Œ ą6 2
A*1104 + A*2601 = A*110101 + A*2609 B
1107/519
A*2428 + A*2601 = A*2404 + A*2603
C
59/133
ë%D£E®µm7 Jab!÷r
A*2432 + A*2601 = A*2404 + A*2501
DRB1 2020/329
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