◊✲άື 13．研究活動 ICG ࢆ⏝࠸ࡓࣜࣥࣃ⠇⤌⧊ࡢ⺯ග⏬ീฎ⌮ศᯒ Image Analysis and Processing of Indocyanine Green(ICG) in lymph node Tissue ᮤ㯪᫂ 㸦Liming LI㸧 Tel & Fax: 0123-27-6092 E-mail: [email protected] 㻌 The new system developed by the Li Laboratory uses Indocyanine Green (ICG) with excitation wavelength of 785 nm and fluorescence emission wavelength about 830nm.Then use CCD camera and Near-infrared camera to get real time color video and near-infrared video. With using image analysis and processing by computer, doctors can find sentinel lymph node easily. With C# language, the main work of this research is image analysis. With acquisition of color video, near-infrared video and spectrum data, observe and analyze the lymph node tissue in real time. ⫶⒴ࣜࣥࣃ⠇デ᩿⏝㏆㉥እ⥺⺯ග⏬ീ⭡⭍㙾ࢩࢫࢸ࣒ࡣᮤ◊✲ᐊ࡚㛤Ⓨࡉࢀ࡚࠸ࡿࠋ ࡇࡢࢩࢫࢸ࣒ࡼࡾࠊ࣓࡛࢝ࣛᙳࡋࡓࣜࣝࢱ࣒ࡢ⏬ീࢆ⏬ീฎ⌮ࡍࡿࡇ࡛ࠊ་ᖌ ࡣᐜ᫆ࣜࣥࣃ⠇ࢆⓎぢࡍࡿࡇࡀ࡛ࡁࡿࠋᮏ◊✲ࡣ C#ࢆ⏝࠸ࡓ⏬ീฎ⌮ࢆ⾜࠸ࠊྍ ど⏬ീ㏆㉥እ⥺⺯ග⏬ീࢆྜᡂࡋࠊICG ࡢ⺯ගࢫ࣌ࢡࢺࣝྠ୍ࡢ⏬㠃⾲♧࡛ࣜࣝࢱ ࣒⏕య⤌⧊ࢆほ ࡍࡿࠋᐇ㦂ࡣ㇜⫶⤌⧊ࢆ⏝ࡋࡓࠋ㇜⫶⤌⧊ࡢ⫶๓ቨ₢⭷ୗᒙ ICG ⁐ᾮ(⃰ᗘ 110-4M)ࢆὀᑕࡋࠊࣜࣥࣃ⤌⧊ὶࢀ࡚࠸ࡿ ICG ⁐ᾮࢆ༙ᑟయ࣮ࣞࢨ(Ἴ㛗: 785nm ࣃ࣮࣡: 50mW)࡛ບ㉳ࡉࡏࠊ࣓࣮ࢪࣥࢢࣇࣂ㏆㉥እ⥺࣓࢝ࣛࡼࡗ࡚ࠊࣜ ࣝࢱ࣒ࡢ㏆㉥እ⥺⺯ග⏬ീࢆᙳࡋࡓ㸦ᅗ㸯bࠊ▮༳ࡀࣜࣥࣃ⠇࡛࠶ࡿ㸧 ࠋLED ග※ࡢ↷ ᑕࡼࡾࠊྍどࡢ⏬ീࡶᙳࡋࡓ㸦ᅗ㸯a㸧ࠋࡲࡓࠊICG ࡢ⺯ගࢫ࣌ࢡࢺࣝࢆศගჾ CCD ᳨ฟჾ࡛ྲྀᚓࡋࡓ㸦ᅗ␎㸧 ࠋࡑࡋ࡚ࠊࡘࡢ⏬ീࢆྜᡂࡋࠊ⺯ගࢫ࣌ࢡࢺࣝྠࠊࣜࣥ ࣃ⤌⧊ෆࡢ ICG ࡢ⺯ගࢆほ ࡍࡿࡇࡼࡾࠊࣜࣥࣃ⠇ࡢ⨨ࡢほ ࡀฟ᮶ࡓ㸦ᅗ㸯c㸧 ࠋ ྍど⏬ീࡢ⏕ࢫࣆ࣮ࢻࡣ⣙ 10fps(Frames Per Second)࡛࠶ࡾࠊ㏆㉥እ⥺⺯ග⏬ീࡢ⏕ࢫࣆ ࣮ࢻࡣ⣙ 7fps ࡛࠶ࡿࠋྜᡂ⏬ീࡢ⏕ࢫࣆ࣮ࢻࡣ⣙ 5fps ࡛࠶ࡿࠋ᭦ࠊ㏆㉥እ⥺⺯ග⏬ീ ࡼࡾࠊࣜࣥࣃ⟶ࡢὶࢀࡶ᫂☜ほ ࡛ࡁࡓࠋ a b c Fig.1 Color video(a) near-infrared video(b) and composite video(c) of ICG in lymph node ࠙ᩥ⊩ࠚS. Kaneshiro, Visual C# 2010 Perfect master―MICROSOFT VISUAL STUDIO 2010 , Shuwasystem, 2011 - 152 - − 152 −
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