Anti-PLK1 antibody [Mixed clones, 3G251]

Product datasheet
Anti-PLK1 antibody [Mixed clones, 3G251] ab14210
2 Abreviews 6 References
画像数 2
製品の概要
製品名
Anti-PLK1 antibody [Mixed clones, 3G251]
製品の詳細
Mouse monoclonal [Mixed clones, 3G251] to PLK1
特異性
This antibody reacts specifically with the 68 kDa Plk1 protein. However, cross-reactivity with a
110 kDa protein of unknown identity is sometimes observed.
アプリケーション
適用あり : ICC/IF, Functional Studies, ELISA, IP, WB
種交差性
交差種 : Mouse, Rat, Human, Xenopus laevis
交差が予測される動物種 : Pig
免疫原
Fusion protein:
DPACIPIFWVS KWVDYSDKYG LGYQLCDNS
VGVLFNDST RLILYNDGDSL QYIERDGTESY
LTVSSHPNSLM KKITLLKYFRN YMSEHLLKAGA
NITPREGDELA RLPYLRTWF RTRSAIILH
LSNGSVQINFF QDHTKLILCP LMAAVTYIDE
KRDFRTYRLS LLEEYGCCKE LASRLRYARTM
VDKLLSSRSAS NRLKAS
, corresponding to amino acids 402-603 of Human Plk1.
Run BLAST with
Run BLAST with
ポジティブ･コントロール
HeLa, A431 and NIH 3T3 cells.
特記事項
Isotype is IgG1 kappa and IgG2b kappa. For maximum recovery of product, centrifuge the
original vial after thawing and prior to removing the cap. Further dilutions can be made in assay
buffer.
ab14210 has been shown to preferentially detect centrosomal-associated Plk1. An alternative
antibody, Rabbit polyclonal to Plk1 (ab14209) can be used to detect kinetochore-associated
Plk1.
製品の特性
製品の状態
Liquid
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
バッファー
Preservative: 0.05% Sodium Azide
Constituents: 40% Glycerol, PBS, pH 7.4
精製度
Protein A purified
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一次抗体備考
ab14210 has been shown to preferentially detect centrosomal-associated Plk1. An alternative
antibody, Rabbit polyclonal to Plk1 (ab14209) can be used to detect kinetochore-associated
Plk1.
ポリ /モノ
モノクローナル
クローン名
Mixed clones, 3G251
アイソタイプ
IgG
軽鎖の種類
kappa
アプリケーション
Our Abpromise guarantee covers the use of ab14210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション
Abreviews
特記事項
ICC/IF
Use at an assay dependent concentration.
Functional Studies
Use at an assay dependent dilution. PubMed: 20427271In vitro kinase assay.
ELISA
Use a concentration of 0.1 - 1 µg/ml.
IP
Use a concentration of 2 - 5 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 68 kDa
(predicted molecular weight: 67 kDa).
ターゲット情報
機能
Serine/threonine-protein kinase that performs several important functions throughout M phase of
the cell cycle, including the regulation of centrosome maturation and spindle assembly, the
removal of cohesins from chromosome arms, the inactivation of APC/C inhibitors, and the
regulation of mitotic exit and cytokinesis. Required for recovery after DNA damage checkpoint
and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1.
Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its
centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of
BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through
phosphorylation of TOPORS.
組織特異性
Placenta and colon.
配列類似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC5/Polo subfamily.
Contains 2 POLO box domains.
Contains 1 protein kinase domain.
発生段階
Accumulates to a maximum during the G2 and M phases, declines to a nearly undetectable level
following mitosis and throughout G1 phase, and then begins to accumulate again during S
phase.
翻訳後修飾
Catalytic activity is enhanced by phosphorylation of Thr-210. Phosphorylation at Thr-210 is first
detected on centrosomes in the G2 phase of the cell cycle, peaks in prometaphase and
gradually disappears from centrosomes during anaphase.
Autophosphorylation and phosphorylation of Ser-137 may not be significant for the activation of
PLK1 during mitosis, but may enhance catalytic activity during recovery after DNA damage
checkpoint.
Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) in anaphase and
following DNA damage, leading to its degradation by the proteasome. Ubiquitination is
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mediated via its interaction with FZR1/CDH1. Ubiquitination and subsequent degradation
prevents entry into mitosis and is essential to maintain an efficient G2 DNA damage checkpoint.
細胞内局在
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > centrosome.
During early stages of mitosis, the phosphorylated form is detected on centrosomes and
kinetochores. Localizes to the outer kinetochore. Presence of SGOL1 and interaction with the
phosphorylated form of BUB1 is required for the kinetochore localization.
Anti-PLK1 antibody [Mixed clones, 3G251] 画像
Human U2OS cells were fixed for 10 minutes
in 3.7% formaldehyde (30mM sucrose) and
permeabilized for 5 minutes in PBS-Triton
(0.1%). The fixed cells were blocked for 30
minutes then incubated for 40 minutes with
3µg/ml anti-Pkl1, ab14210, (green) and antiAnti-PLK1 antibody [Mixed clones, 3G251]
Centrin (red). The DNA was counterstained
(ab14210)
with DAPI (blue). All incubations were carried
This image is courtesy of Giulia Guarguaglini and Maria
De Luca
out at room temperature.
The staining pattern shows that ab14210
localizes to the centrosomes during most of
mitosis (upper panel) and the midbody during
telophase (lower panel). The centrosomal pool
of Plk1 can be seen by comparing with centrin,
a centriolar marker. An alternative antibody,
Rabbit polyclonal to Plk1 (ab14209) can be
used to selectively stain the kinetochoreassociated Plk1.
Human U2OS cells were fixed for 10 minutes
in 3.7% formaldehyde (30mM sucrose) and
permeabilized for 5 minutes in PBS-Triton
(0.1%). The fixed cells were blocked for 30
minutes then incubated for 40 minutes with
3µg/ml anti-Pkl1, ab14210, (green) and antiCentrin (red). The DNA was counterstained
with DAPI (blue). All incubations were carried
out at room temperature.
3
The staining pattern shows that ab14210
localizes to the centrosomes during most of
mitosis (upper panel) and the midbody during
telophase (lower panel). The centrosomal pool
of Plk1 can be seen by comparing with centrin,
a centriolar marker. An alternative antibody,
Rabbit polyclonal to Plk1 (ab14209) can be
used to selectively stain the kinetochoreassociated Plk1.
4
Human U2OS cells were fixed and
permeabilized in 4% formaldehyde, 0.2%
Triton X100.The fixed cells were blocked for
30 minutes then incubated for 40 minutes with
3µg/ml anti-Pkl1, ab14210, (green) and antiCentrin (red). The DNA was counterstained
Anti-PLK1 antibody [Mixed clones, 3G251]
with DAPI (blue). All incubations were carried
(ab14210)
out at romm temperature.
This image is courtesy of Giulia Guarguaglini and Maria
De Luca
The staining pattern shows that ab14210
localizes to the centrosomes during most of
mitosis (upper panel) and the midbody during
telophase (lower panel). The centrosomal pool
of Plk1 can be seen by comparing with centrin,
a centriolar marker. An alternative antibody,
Rabbit polyclonal to Plk1 (ab14209) can be
used to selectively stain kinetochoreassociated Plk1.
Human U2OS cells were fixed and
permeabilized in 4% formaldehyde, 0.2%
Triton X100.The fixed cells were blocked for
30 minutes then incubated for 40 minutes with
3µg/ml anti-Pkl1, ab14210, (green) and antiCentrin (red). The DNA was counterstained
with DAPI (blue). All incubations were carried
out at romm temperature.
The staining pattern shows that ab14210
localizes to the centrosomes during most of
mitosis (upper panel) and the midbody during
telophase (lower panel). The centrosomal pool
of Plk1 can be seen by comparing with centrin,
a centriolar marker. An alternative antibody,
Rabbit polyclonal to Plk1 (ab14209) can be
used to selectively stain kinetochoreassociated Plk1.
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