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微生物 NAD キナ の構造と機能に関する研究 受 受賞者講演要旨 《農芸

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NAD NAD(H) [NADῌ NADH] !"#$%&'( )*+,-.
gomonas sp. A1 Š( <&Z< !‹`;t~ Methanococcus
jannaschii( „…9 !: Saccharomyces cerevisiae(Œ ;
NADῌ /0)1234 ADP 456$789:;
< NADK +=KŽ* NADK /,+x/7
<=>[email protected]?DE,-. F
$>?h4;apb
NAD(H) G4;AH- NADP(H) [NADPῌ NADPH]
h. ,?i ῌ{|}3Rt~<&Z< NADK @
[email protected]
?I J ?-+KL1L?-. NADP(H) Pn ATP K NAD (H) K 4 ; , - Pn/ATP-
!M( 79:
NAD(H) Z [ & \ [NAD (H) K] V W - X ῍ „ … N9OPQE,-.
NADK ATP ‘K NAD(H) K4;,- ATP-
NAD(H) NADP(H) ?RS+T*
NAD(H)K VW-X ῎{|}‰Rt~ NADK ATP
OP NAD(H)/NADP(H) UQRVW-. X
‘K NADῌ ‘K4;,- ATP-NADK VW
UQY?-G NAD Z[&\ (NADK) NADP(H)
-XK’h ‡4;b
]^9]_`&\ [NADP(H)ase] VW-. NADK 4;a
@ NADK NAD (H) K K•B,-Œ !– 1(. —OD
[ATP 7344; (Pn)] K4;b NADῌ
E. coli NADK(YfjB) ‘GCD NADP(H) V<˜9
ῌ
@
RT*K“A,-”
K4; NADP KM,-. NADP(H)ase NADP(H)
™4š›EœKb-X )12: NADK žFŠGGŸ
K NAD (H) c 4 ; , -. ATP d NADK RK’,[email protected]’ t)* NAD(H)ῌNADP(H) UQ
1950 ! Pn d NADK 1980 !eHfH"gA
+xe+K,-L V?zH%gK
H*h. O NADP(H) RDOOiPj k#l
h. AP NADK F¡x/<|¢;‹;:1IIJK
Kmn o?p$l%gGJ
£ A )12 B Kg*¤h. XHP¥ NADK x/
PH*?Oqh. NADP(H)ase E er,P&
p$lK"LA¦-z1%g?q
VWqh.
*-.
'1I NADK NADP(H)ase k#lGsn
?) Pn §$:4;G4;"9™$ˆMV¨M
(AH t)* NAD(H)/NADP(H) +,-?.uv
h©"#$%&MVW-. ATP ª-O«¬
EwK x/)12$ly0OP,-z1
V M­ (ADP) G®?* !ATP K*h¯M
Go2AHh.
­N+ ADP G®-(. eXV ˆ…~ Pn/ATP-
1.
NADK !"
{|}3Rt~ Micrococcus flavus 1I Pn ATP NADK(Ppnk) Pn K*h©°D NADPῌ «¬?+
­OK±² ³­)- Pn "#$%&a
K,- Pn/ATP-NADK K$4 e<056€
PRKsn’h.
7Kz sn NADK k#l(h. XH1
2.
I ‚0}56ƒ%„8t~ „… )12<&Z<
NADP(H)ase [email protected]
? NADP(H)ase ´RK’,<$µ4]^9]_
NADK (?P2 NADK x/ p $l
`&\[email protected]%PH*hG NADP(H) 4;
G†?qh.
1 I NAD(H)/NADP(H) U Q O O i - ¶„ ·
Bj ,-?9 ‡{|}3Rt~ !ˆ…~ Mycobacterium tuberculosis( {|}‰Rt~ !Escherichia coli, Sphin-
NADP(H)ase &VWqh.
' ) * < & Z < M. jannaschii ; < NADK
# 1 ,-?9;< NADK /,+x/4;apb @ R
24
ºn²FG„Ž»
5Ž\¼½,›
3 Ppnk + NADῌ 0a
™¸· qwxIfyz$ Asp189, Gly187 ^¹š
Arg187 =H‡_ >[email protected]=#$$
*+l|3}& ~Ja+If49g NADK 2 Ppnk +0-Q ^˜B-_
T.¶l· AῌA῍U> BῌB῍AB-+nopqrs+
³$
NAD(H)K +b456-|)`1:Q,d[
T"#$ && a|K|=7R& NADK =6
*>& ~Ja+€L
>MNIf4 NADῌ ‚
(MJ0917) NADK +|)ƒ1:)'
$ U& O„…+†P&
(IMPase) ~Ja+ Gly U>vIf4‚+ 1 If4H‡=7
!"#$ %& IMPase NADP(H)ase NADK <; NAD(H)K ‚+K‡=ˆ$ W+(
'()*& MJ0917 +,-)./01&
<;& NADK =6‰ NADῌ 0a+eb456
IMPase & IMPase 2)"3 4++& 5
-|==:& AB-CnopqrstuD+,
NADP(H)ase 678
-1,6-9
)
Š=& NADK QB-Q)‹Œ& :3cDR
(FBPase) 2):
;<=3/$ MJ 0917 >&
B-+ NADK ;H3 ‹Œ)$
NADPῌ +-
':2)4?
NF& Pn SD,Q-
+ŽT<;& Pn/ATP-NADK U
@NADK NADP(H)aseA <;3&
= Pn SDViLWXY#$ Pn/ATP-
wJ
NADῌ/NADPῌ )BCD'E+FG)H
+] ^
wb4+-_ +0-Q
* )$ ";=& +IJI+KL
)`1& Pn 0a+Q Pn SD,Q ^X!Y]SD,
>& NADK M IMPase MN
Q_ )
;<=$ 36& XY#$
wJ)Z
OP+Q)/*6R& IMPaseNADP(H)
+#$‘J’J>U“k ^
w+b4F_ )
+S!)T$ U& "+IJI IMPase Z[:& %\+N<Q+] U+}& %J
M. jannaschii # $ MJ 0109 Archaeoglobus
+Q^F+S+_`>ab+/$ XY#$
fulgidus #$ AF2372 4& IMPase FBPase %2+V<=
Pn/ATP-
wJ+0-Q)c}*
;<=& %
NADP(H)ase 2):)&'$ W+(>& )*
J+Q+S)c}*&'& %J+Q
XY)Z++ IMPase NADPase '
^F=<<cde+ab=`f)?‰$
,3[4T$
3.
NADK Pn O„…>& ”ghG ^•_ij–G„…kij—lSDm6
ῌ
0ZY Pn/ATP-NADK(Ppnk) +I\]] ^NAD
+-_ + X .0/Q ^0-Q_ )`1 ^2 2_&
ῌ
78”ghGhG˜nG„…–io+–Gp™+,K‡
Gm=6 *.c#4+$ qršst& šuv)
NAD 0a+3XQb456-78=<<cQ
!
,d)-$ NADῌ 0a+e=<<cIf
:$ U& O„…=S*šstC!" U”ghGhG
49g>& :h*+ NADK =B:=;"#*6R& N<Q
˜{›w žO |z& Q-=6 *šst
”ghGhG˜›w
xyœuz=
U
+Iij<;
;<=; A 678 B =
}RU”ghGhG˜kD~–Gp™›w ŸŸz
=k* $ U& ]+-=7R& Iij+
=6€ U:$ O„…=š¡‚ !mU\y
l<;>1m3 ; NE/D short motif ) !
„…¢& „…& Gƒ£& ¤¥+¦U„…\+§=
$ #;+;9g+>& =& Asp189 >[email protected]
U:$ ¨e=cR©„) !mUª…”ghG†‡
qr#$R ^2 3_& Ppnk +AB- ^AῌA῍U> BῌB῍:
›w ^« ˆ‰¬0hG›w_ ­Š®‹z& ”ghGhG˜
2 2_ C+nopqrstuD=SE$ NF& Gly187
›w ŒO¯Az= U:$ °±=3RU& O
>& NADK NAD(H)K =6
*=;"#*6R
„Ž=šC!" U‘On²FG³S’“d+”N
@NADK, Arg; NAD(H)K, Gly U>vIf4A& [email protected]
•z ^”ghGhG˜›w_ 3;8=wŽ–´µ¢+ƒz
pqr+ NADῌ ^=qwxIfyz_ 0a+{G=
F=—C6€ U:$
ῌ
aHI ^2 3_$ NAD NADH qwxIfyz=6
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