S1 Table-1: Metabolites in PEHM2 and HM-4 extracts from maize plants that were significantly different under low-P and sufficient-P
conditions and were tentatively identified using GC-MS
Spectru
m No.
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
tR (min)
27.442
17.865
37.395
35.705
18.624
38.715
26.014
16.908
6.23
9.210
15.223
13.893
14.831
16.796
10.041
17.176
17
18
19
20
21
16.850
18.625
40.336
19.847
29.712
Metabolites
Mannose
Ribose
Turanose
Sucrose
Ketocose
Maltose
Lactose
Raffinose
Glycine
Serine
Asparagine
Aspartic acid
Proline
Glutamine
Isoleucine
γ-aminobutyric acid
Phenylalinine
Glucose -PO4
Fructose-PO4
Phosphoric
acid
Inositol-PO4
m/z
Peak no.
ID
M.F
204
217
361
362
103
204
205
171
174
116
188
144
156
158
86
117
166
81
214
167
108
199
155
95
14
31
72
62
68
83
37
85
NIST05 Lib
WILEY8 Lib
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
NIST05 Lib
NIST05 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
NIST05 Lib
C18H44O5Si4
C17H42O5Si4
C33H78O11Si7
C25H45O6Si8
C20H48N2O6Si4
C36H86O11Si8
C36H86O11Si8
C11H22B2O6Si
C11H29NOSi3
C9H23NO3Si2
C16H40NO3Si2
C16H28N2O6
C11H23NO3Si2
C14H34N2O3Si3
C8H17NO2
C10H25NO2Si2
Derivatize
d
TMS
TMS
TMS
TMS
TMS
TMS
TMS
TMS
TMS
TMS
(TMS)3
TMS
TMS
(TMS)3
TMS
TMS
Related metabolism
218
387
315
211
81
97
241
106
NIST05 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
C15H27NO2Si2
C25H64NO9Psi6
C25H64NO9PSi6
C9H22NO4PSi2
TMS
TMS
TMS
TMS
Amino acid metabolism
Glycolisis metabolism
Glycolisis metabolism
Phosphate metabolism
318
182
WILEY8 Lib
C27H69O9PSi4
TMS
Phosphate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Carbohydrate metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
Amino acid metabolism
22
23
24
25
26
27
28
29
30
31
32
33
34
28.363
39.483
22.984
22.300
38.466
37.120
15.983
20.124
28.745
19.930
26.101
11.299
23.764
35
29.178
36
20.593
37
15.848
Glycerol-3PO4
Cadaverine
Thymol
D-Glucitol
Tocopherol
Mannitol
Ketoglutaric
acid
Malonic acid
Gulonic acid
Azelaic acid
Oxalic acid
Maleic acid
Indole-3acetic acid
D-glucuronic
acid
Tricarboxylic
acid
Trihydroxy
butyric acid
38
39
40
41
23.979 Cinnamic acid
Quinolinic
38.563 acid
24.356 Glurcaric acid
21.008 Succinate
147
173
WILEY8 Lib
C12H31NO3PSi3 (TMS)3
NIST05 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
C17H46N2Si4
C28H56O6Si4
C15H32O6
C32H58O2Si
C14H30O5
C12H25NO5Si
TMS
TMS
TMS
TMS
TMS
(TMS)2
Glycerolipid, Carbohydrate
metabolism
Lipid metabolism
Sugar alcohol metabolism
Sugar alcohol metabolism
Sugar alcohol metabolism
Sugar alcohol metabolism
TCA cycle metabolism
174
361
103
502
43
147
134
128
224
213
68
148
217
317
57
146
232
89
175
87
145
34
117
NIST05 Lib
NIST05 Lib
WILEY8 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
C13H28O4Si2
C18H42O6Si4
C15H32O4Si2
C11H20O4Si2
C10H20O4Si2
C17H27NO3Si2
TMS
TMS
TMS
TMS
TMS
TMS
Lipid metabolism
Lipid metabolism
Lipid metabolism
TCA cycle metabolism
TCA cycle metabolism
Lipid metabolism
218
175
NIST05 Lib
C21H50O7Si5
TMS
Lipid metabolism
273
114
WILEY8 Lib
C18H40O7Si4
TMS
TCA cycle metabolism
292
76
NIST05 Lib
C16H40O5Si4
TMS
Lipid metabolism
208
132
NIST05 Lib
C11H12O4
TMS
167
225
NIST05 Lib
C9H14O2Si
TMS
149
147
144
WILEY8 Lib
WILEY8 Lib
C24H58O8Si6
C11H24O4Si2
TMS
TMS
Secondary metabolite
metabolism
Secondary metabolite
metabolism
TCA cycle metabolism
TCA cycle metabolism
42
43
44
45
46
47
48
49
25.610
26.442
19.718
19.611
41.045
41.197
26.334
Mannoic acid
Linolenic acid
Parabanic acid
Isocitric acid
Cholesterol
Stigmasterol
Linoseaure
204
75
100
273
386
83
75
596
142
148
116
103
193
147
173
WILEY8 Lib
NIST05 Lib
NIST05 Lib
NIST05 Lib
NIST05 Lib
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
C22H53NO7Si5
C21H38O2Si
C9H18N2O3Si2
C18H40O7Si4
C27H47O
C29H48O
C21H40O2Si
C42H60O2
TMS
TMS
TMS
(TMS)2
TMS
TMS
TMS
TMS
139
148
WILEY8 Lib
C11H23O2Si2
TMS
167
219
WILEY8 Lib
C12H11F8N2
TMS
230
217
45
142
181
201
26
99
NIST05 Lib
WILEY8 Lib
WILEY8 Lib
NIST05 Lib
C22H45N5O4Si4
C18H36N2O6Si3
C18H45N3O3Si4
C17H44N2O2Si4
(TMS)4
(TMS)4
(TMS)2
TMS
43.581 Spirilloxanthin
50
51
52
53
54
55
29.369 Oxiran
Oxacyclotetra37.984 5-yn-2-one
32.729 Adenosine
29.913 Uridine
11.771 Citrulline
20.366 L-Ornithine
Lipid metabolism
Lipid metabolism
Lipid metabolism
TCA cycle metabolism
Lipid metabolism
Lipid metabolism
Lipid metabolism
Secondary metabolite
metabolism
Secondary metabolite
metabolism
Secondary metabolite
metabolism
Nucleic acid metabolism
Nucleic acid metabolism
N2 metabolism
N2 metabolism
tR -Retention time in minutes.
ID- Identification by either commercially available standard compounds in comparison with mass spectra and NIST05 and WILEY8 library
M.F- Molecular formula of the identified compounds
TMS- Derivatization was done with the help of N-Methyl-N-(trimethylsilyl)trifluoroacetamide (MSTFA)