Press Release
BERTHOLD TECHNOLOGIES GmbH & Co.KG
Calmbacher Str.22
75323 Bad Wildbad
Deutschland
http://www.berthold.com/bio
[email protected]
Tel.: +49-7081-177-0
Fax: +49-7081-177-100
Mithras2 – Multi-Technology Microplate Reader with
Monochromators
Bad Wildbad, Germany, April 2014
Berthold Technologies introduces the second generation
of its Multimode Microplate Reader Mithras, the Mithras2
LB943 with monochromator and filter technology for
these reading technologies

UV/VIS Absorbance

Fluorescence and FRET

Fluorescence Polarisation

Time-Resolved Fluorescence (TRF and TR-FRET)

Luminescence

BRET and BRET²

AlphaScreen® and AlphaLISA®
Already implemented in the TriStar² the patent-pending optical concept ONE-4-ALL is employed for
sensitive luminescence, fluorescence and absorbance measurements – with performances as in
dedicated instruments – in the Mithras² for filter-based reading. Additionally the instrument is
equipped with two double monochromators with high blocking efficiency and high f-number
(increased transmission) for filter-less measurements of absorbance and fluorescence.
For utmost sensitivity in luminescence a third specially selected and extremely noise-reduced
detector is engaged in the HiSens position which is used for Alpha and BRET readings, too.
Compared to its predecessor the optics has been improved once again to achieve improved
detection limits

Less than 2 amol ATP per well

Less than 0.2 fmol Fluorescein per well

Less than 2 amol Europium per well
Mithras² offers the possibility to use optical filters for luminescence
measurements (of course, for all fluorescence and absorbance readings, too)
enabling the sensitive measurement of BRET (e.g. functional assays in GPCR
research) and multi-coloured luciferases (reporter gene assays). Each
Seite 2
individual filter is coded with an RFID tag and can be positively identified – a significant contribution
to correct measurements and to secure quality standards.
Up to 4 reagent injectors with the proven and highly accurate JET technology complete the unit. The
injector tips are located in reading position
enabling the accurate measurement of very
fast reactions. A new feature is the reagent
compartment at the front which keeps the
reagents within easy reach and which can be
filled with ice to keep them cooled. Reagent
mounts hold small reagent containers safely
in place and a slight inclination of the mount ensures tubings recover the last droplet.
Cellular assays are taken account of by a universal bottom reading function, a temperature control
unit with an incubation compartment for the microplate and gas connectors.
Thus, the Mithras2 is a microplate reader offering comprehensive possibilities and a multitude of
applications. The measurement of enzyme activities, phagocytosis, calcium flux, cell viability,
apoptosis, immunoassays protein and nucleic acid concentrations and protein-protein interactions
are only a few of the manifold fields of application the instrument offers.
The unit is operated with the approved Mikrowin software. Single and multiple endpoint
measurements can be performed as well as kinetic and scanning measurements. The data can be
displayed numerically and graphically and may be exported to EXCEL or printed.
About Berthold Technologies
Berthold Technologies, a family driven company with its headquarters in Bad Wildbad, Germany,
has been providing bioanalytical instruments to the life sciences market for more than 60 years.
The company is offering innovative products through a worldwide network of subsidiaries and
distributors to customers in academic and pharmaceutical research as well as clinical diagnostics.
Contact
Bernd Hutter
BERTHOLD TECHNOLOGIES GmbH & Co. KG
[email protected]
phone: +497081177-0
www.Berthold.com/bio