Document

BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 1 / 11 – Dec 20, 2009
Model and Description
LB 941 TriStar Multimode Reader
platform for these reading technologies:
absorption
flash luminescence
glow luminescence
colour luminescence (Chroma-Glo™)
BRET (Bioluminescence Resonance Energy Transfer)
fluorescence intensity top reading
FRET (Fluorescence Resonance Energy Transfer)
optimum performance and sensitivity due to
dedicated optical system for each reading technology (DOPS)
manual selection of optical path
clear and opaque 96 and 384 well microplates
(maximum dimensions w x l 86.0 mm x 128.2 mm;
H 15 +/- 1 mm)
up to 4 additional automatic injectors
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
plate shaking: 3 modes (linear, orbital and double orbital),
3 velocities
well scanning w/ up to 10,000 individual
reading points per well
reading time per well adjustable via software:
between 0.05 and 600 sec
excitation unit:
Tungsten halogen lamp, 75 W, spectral range 340–700 nm,
typical life time >300 h
filter slide w/ 5 positions (Ø 15 mm)
detection unit:
photomultiplier tube (low noise photocathode, high
cathode sensitivity, digital photon counting mode)
silicon diode, spectral range 200-1000 nm
emission filter wheel w/ 8 positions (Ø 25,4 mm)
performance:
luminescence
spectral range 340 - 650 nm
detection limit for ATP <6 amol/well
detection limit for Firefly Luciferase <3 zmol/well
fluorescence (top)
spectral range 340 - 680 nm
detection limit for Fluorescein <0.3 fmol/well (384)
absorbance
spectral range 340 - 700 nm
0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm)
precision <0,6 % cv (@ 2.5 OD)
accuracy <2 % cv (@ 2.5 OD)
Total Amount Net
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 2 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
MikroWin Lite software
instrument control
definition of reading times and injection volumes
set-up of plate layout and selection of plate format
selection and definition of special reading sequences like
e.g. dual ratio readings, short term and long term kinetics
control of priming and cleaning sequences
data evaluation
free plate layout (samples, standards, controls)
assignment of sample names
input of dilution factors
qualitative cut-off determination w/ up to 3 categories
kinetics (delta values, min/max values, Vmax, AUCs, time values)
combination of different evaluations
free input of user defined formula
calculation in up to 5 matrices
results & export
display in multi-matrix format or in list format
graphic display of data
integrated statistical functions (e.g. average, standard deviation)
matrix or list format of all raw data and calculated data
on demand or automatically
export as file (LAN connection possible) or to printer
The operation of this system requires a computer system
LB 941 TriStar Multimode Reader
Order No. 49178-10
LB 941 TriStar T Multimode Reader
platform for these reading technologies:
absorption
flash luminescence
glow luminescence
colour luminescence (Chroma-Glo™)
BRET (Bioluminescence Resonance Energy Transfer)
fluorescence intensity top reading
FRET (Fluorescence Resonance Energy Transfer)
optimum performance and sensitivity due to
dedicated optical system for each reading technology (DOPS)
manual selection of optical path
clear and opaque 96 and 384 well microplates
(maximum dimensions w x l 86.0 mm x 128.2 mm;
H 15 +/- 1 mm)
up to 4 additional automatic injectors
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
plate shaking: 3 modes (linear, orbital and double orbital),
€
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 3 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
3 velocities
well scanning w/ up to 10,000 individual
reading points per well
reading time per well adjustable via software:
between 0.05 and 600 sec
temperature control user defined
between 5 °C above RT and 42 °C
incl. Peltier cooling unit for stabilisation of photocathode
excitation unit:
Tungsten halogen lamp, 75 W, spectral range 340–700 nm,
typical life time >300 h
filter slide w/ 5 positions (Ø 15 mm)
detection unit:
photomultiplier tube (low noise photocathode, high
cathode sensitivity, digital photon counting mode)
silicon diode, spectral range 200-1000 nm
emission filter wheel w/ 8 positions (Ø 25,4 mm)
performance:
luminescence
spectral range 340 - 650 nm
detection limit for ATP <6 amol/well
detection limit for Firefly Luciferase <3 zmol/well
fluorescence (top)
spectral range 340 - 680 nm
detection limit for Fluorescein <0.3 fmol/well (384)
absorbance
spectral range 340 - 700 nm
0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm)
precision <0,6 % cv (@ 2.5 OD)
accuracy <2 % cv (@ 2.5 OD)
MikroWin Lite software
instrument control
definition of reading times and injection volumes
set-up of plate layout and selection of plate format
selection and definition of special reading sequences like
e.g. dual ratio readings, short term and long term kinetics
control of priming and cleaning sequences
data evaluation
free plate layout (samples, standards, controls)
assignment of sample names
input of dilution factors
qualitative cut-off determination w/ up to 3 categories
kinetics (delta values, min/max values, Vmax, AUCs, time values)
combination of different evaluations
free input of user defined formula
calculation in up to 5 matrices
results & export
display in multi-matrix format or in list format
graphic display of data
integrated statistical functions (e.g. average, standard deviation)
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 4 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
matrix or list format of all raw data and calculated data
on demand or automatically
export as file (LAN connection possible) or to printer
The operation of this system requires a computer system
LB 941 TriStar T Multimode Reader
Order No. 49178-15
€
LB 941 TriStar i Multimode Reader
platform for these reading technologies:
absorption
flash luminescence
glow luminescence
colour luminescence (Chroma-Glo™)
BRET (Bioluminescence Resonance Energy Transfer)
fluorescence intensity top reading
FRET (Fluorescence Resonance Energy Transfer)
optimum performance and sensitivity due to
dedicated optical system for each reading technology (DOPS)
manual selection of optical path
clear and opaque 96 and 384 well microplates
(maximum dimensions w x l 86.0 mm x 128.2 mm;
H 15 +/- 1 mm)
2 automatic injectors w/ user adjustable volumes (10 – 100 µl)
in luminescence & fluorescence reading positions (#3 and #4)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
up to 2 additional automatic injectors w/
user adjustable volumes (10 – 100 µl)
with wash&prime plate
plate shaking: 3 modes (linear, orbital and double orbital),
3 velocities
well scanning w/ up to 10,000 individual
reading points per well
reading time per well adjustable via software:
between 0.05 and 600 sec
excitation unit:
Tungsten halogen lamp, 75 W, spectral range 340–700 nm,
typical life time >300 h
filter slide w/ 5 positions (Ø 15 mm)
detection unit:
photomultiplier tube (low noise photocathode, high
cathode sensitivity, digital photon counting mode)
silicon diode, spectral range 200-1000 nm
emission filter wheel w/ 8 positions (Ø 25,4 mm)
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 5 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
performance:
luminescence
spectral range 340 - 650 nm
detection limit for ATP <6 amol/well
detection limit for Firefly Luciferase <3 zmol/well
fluorescence (top)
spectral range 340 - 680 nm
detection limit for Fluorescein <0.3 fmol/well (384)
absorbance
spectral range 340 - 700 nm
0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm)
precision <0,6 % cv (@ 2.5 OD)
accuracy <2 % cv (@ 2.5 OD)
MikroWin Lite software
instrument control
definition of reading times and injection volumes
set-up of plate layout and selection of plate format
selection and definition of special reading sequences like
e.g. dual ratio readings, short term and long term kinetics
control of priming and cleaning sequences
data evaluation
free plate layout (samples, standards, controls)
assignment of sample names
input of dilution factors
qualitative cut-off determination w/ up to 3 categories
kinetics (delta values, min/max values, Vmax, AUCs, time values)
combination of different evaluations
free input of user defined formula
calculation in up to 5 matrices
results & export
display in multi-matrix format or in list format
graphic display of data
integrated statistical functions (e.g. average, standard deviation)
matrix or list format of all raw data and calculated data
on demand or automatically
export as file (LAN connection possible) or to printer
The operation of this system requires a computer system
LB 941 TriStar i Multimode Reader
Order No. 49178-20
LB 941 TriStar Ti Multimode Reader
platform for these reading technologies:
absorption
flash luminescence
glow luminescence
colour luminescence (Chroma-Glo™)
BRET (Bioluminescence Resonance Energy Transfer)
fluorescence intensity top reading
FRET (Fluorescence Resonance Energy Transfer)
€
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 6 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
optimum performance and sensitivity due to
dedicated optical system for each reading technology (DOPS)
manual selection of optical path
clear and opaque 96 and 384 well microplates
(maximum dimensions w x l 86.0 mm x 128.2 mm;
H 15 +/- 1 mm)
2 automatic injectors w/ user adjustable volumes (10 – 100 µl)
in luminescence & fluorescence reading positions (#3 and #4)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
up to 2 additional automatic injectors w/
user adjustable volumes (10 – 100 µl)
with wash&prime plate
plate shaking: 3 modes (linear, orbital and double orbital),
3 velocities
well scanning w/ up to 10,000 individual
reading points per well
reading time per well adjustable via software:
between 0.05 and 600 sec
temperature control user defined
between 5 °C above RT and 42 °C
incl. Peltier cooling unit for stabilisation of photocathode
excitation unit:
Tungsten halogen lamp, 75 W, spectral range 340–700 nm,
typical life time >300 h
filter slide w/ 5 positions (Ø 15 mm)
detection unit:
photomultiplier tube (low noise photocathode, high
cathode sensitivity, digital photon counting mode)
silicon diode, spectral range 200-1000 nm
emission filter wheel w/ 8 positions (Ø 25,4 mm)
performance:
luminescence
spectral range 340 - 650 nm
detection limit for ATP <6 amol/well
detection limit for Firefly Luciferase <3 zmol/well
fluorescence (top)
spectral range 340 - 680 nm
detection limit for Fluorescein <0.3 fmol/well (384)
absorbance
spectral range 340 - 700 nm
0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm)
precision <0,6 % cv (@ 2.5 OD)
accuracy <2 % cv (@ 2.5 OD)
MikroWin Lite software
instrument control
definition of reading times and injection volumes
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 7 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
set-up of plate layout and selection of plate format
selection and definition of special reading sequences like
e.g. dual ratio readings, short term and long term kinetics
control of priming and cleaning sequences
data evaluation
free plate layout (samples, standards, controls)
assignment of sample names
input of dilution factors
qualitative cut-off determination w/ up to 3 categories
kinetics (delta values, min/max values, Vmax, AUCs, time values)
combination of different evaluations
free input of user defined formula
calculation in up to 5 matrices
results & export
display in multi-matrix format or in list format
graphic display of data
integrated statistical functions (e.g. average, standard deviation)
matrix or list format of all raw data and calculated data
on demand or automatically
export as file (LAN connection possible) or to printer
The operation of this system requires a computer system
LB 941 TriStar Ti Multimode Reader
Order No. 49178-21
€
LB 941 TriStar vTi Multimode Reader
platform for these reading technologies:
absorption
flash luminescence
glow luminescence
colour luminescence (Chroma-Glo™)
BRET (Bioluminescence Resonance Energy Transfer)
fluorescence intensity top reading
FRET (Fluorescence Resonance Energy Transfer)
optimum performance and sensitivity due to
dedicated optical system for each reading technology (DOPS)
manual selection of optical path
clear and opaque microplates (6, 12, 24, 48, 96 and 384 well)
(maximum dimensions w x l 86.0 mm x 128.2 mm;
H 14 mm - 20 mm)
2 automatic injectors w/ user adjustable volumes (10 – 100 µl)
in luminescence & fluorescence reading positions (#3 and #4)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
up to 2 additional automatic injectors w/
user adjustable volumes (10 – 100 µl)
with wash&prime plate
plate shaking: 3 modes (linear, orbital and double orbital),
3 velocities
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 8 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
well scanning w/ up to 10,000 individual
reading points per well
reading time per well adjustable via software:
between 0.05 and 600 sec
temperature control user defined
between 5 °C above RT and 42 °C
incl. Peltier cooling unit for stabilisation of photocathode
excitation unit:
Tungsten halogen lamp, 75 W, spectral range 340–700 nm,
typical life time >300 h
filter slide w/ 5 positions (Ø 15 mm)
detection unit:
photomultiplier tube (low noise photocathode, high
cathode sensitivity, digital photon counting mode)
silicon diode, spectral range 200-1000 nm
emission filter wheel w/ 8 positions (Ø 25,4 mm)
performance:
luminescence
spectral range 340 - 650 nm
detection limit for ATP <6 amol/well
detection limit for Firefly Luciferase <3 zmol/well
fluorescence (top)
spectral range 340 - 680 nm
detection limit for Fluorescein <0.3 fmol/well (384)
absorbance
spectral range 340 - 700 nm
0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm)
precision <0,6 % cv (@ 2.5 OD)
accuracy <2 % cv (@ 2.5 OD)
MikroWin Lite software
instrument control
definition of reading times and injection volumes
set-up of plate layout and selection of plate format
selection and definition of special reading sequences like
e.g. dual ratio readings, short term and long term kinetics
control of priming and cleaning sequences
data evaluation
free plate layout (samples, standards, controls)
assignment of sample names
input of dilution factors
qualitative cut-off determination w/ up to 3 categories
kinetics (delta values, min/max values, Vmax, AUCs, time values)
combination of different evaluations
free input of user defined formula
calculation in up to 5 matrices
results & export
display in multi-matrix format or in list format
graphic display of data
integrated statistical functions (e.g. average, standard deviation)
matrix or list format of all raw data and calculated data
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
page 9 / 11 – Dec 20, 2009
Model and Description
Total Amount Net
on demand or automatically
export as file (LAN connection possible) or to printer
The operation of this system requires a computer system
LB 941 TriStar vTi Multimode Reader
Order No. 49178-25
€
Options and accessories
Injector #1
pre-reading position luminescence
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
Order No.: 37772-11
€
Injector #2
reading position luminescence
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
Order No.: 37772-12
€
Injector #3
reading position luminescence
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
injection into 384 well plates
Order No.: 37772-13
€
Injector #4
reading position fluorescence
w/ user adjustable volumes (10 – 100 µl)
JET Injection for fast and complete reagent mixing
Order No.: 37772-14
€
BRET package
selected filter pairs for BRET and BRTE² assays
400 nm (Deep Blue C™), 515 nm (GFP²),
480 nm (Coelenterazin) and 530 nm (YFP)
Order No.: 39350
BRET1 “high efficiency” package
€
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
Model and Description
page 10 / 11 – Dec 20, 2009
Total Amount Net
Selected BRET filter pairs
480 nm (Coelenterazin) and 540 nm (YFP)
with transmission characteristics
to double signal
for extremely weak experessions
Order No.: 53431
€
BRET² “high efficiency” package
Selected BRET² filter pairs
410 nm (Deep Blue C™) and 515 nm (GFP²)
with transmission characteristics
to double signal
for extremely weak experessions
Order No.: 53432
€
Additional filter slide “B”
for excitation filters
5 positions
for filters with ø 15 mm
Order No.: 41537
€
Additional filter slide “B”
for excitation filters
5 positions
for filters with ø 15 mm
equipped with absorption filters
405 nm, 450 nm, 490 nm, 620 nm
Order No.: 41538
€
Additional filter slide “C”
for excitation filters
5 positions
for filters with ø 15 mm
Order No. 52539
€
Additional filter wheel “B”
for emission filters
8 positions
for filters with ø 25 mm
Order No.: 55384
Additional filter wheel “C”
€
BERTHOLD-BIO
Item
Qty.
Quotation Texts TriStar LB 941
Model and Description
page 11 / 11 – Dec 20, 2009
Total Amount Net
for emission filters
8 positions
for filters with ø 25 mm
Order No.: 55385
€
Chroma-Glo™ package
selected filter pair
510 nm and 610 nm
Order No.: 43544
€
Robot Integration Module
software patch for control and communication
with robot software
for integration of the TriStar into laboratory automation systems
documentation of the communication language
Order No.: 39354sp
€
Prime&Wash Plate
to receive excess liquids
from prime and wash activities
Order No.: 55018
€
Adapter Frame IWAKI clear
for unlidded clear Iwaki plates
to be used with TriStar vTi (49178-25)
Order No.: 54667
€
Adapter Frame Falcon 353075
for lidded BD Falcon 353075
to be used with TriStar vTi (49178-25)
Order No.: 55616
€