BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 1 / 11 – Dec 20, 2009 Model and Description LB 941 TriStar Multimode Reader platform for these reading technologies: absorption flash luminescence glow luminescence colour luminescence (Chroma-Glo™) BRET (Bioluminescence Resonance Energy Transfer) fluorescence intensity top reading FRET (Fluorescence Resonance Energy Transfer) optimum performance and sensitivity due to dedicated optical system for each reading technology (DOPS) manual selection of optical path clear and opaque 96 and 384 well microplates (maximum dimensions w x l 86.0 mm x 128.2 mm; H 15 +/- 1 mm) up to 4 additional automatic injectors w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing injection into 384 well plates plate shaking: 3 modes (linear, orbital and double orbital), 3 velocities well scanning w/ up to 10,000 individual reading points per well reading time per well adjustable via software: between 0.05 and 600 sec excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h filter slide w/ 5 positions (Ø 15 mm) detection unit: photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting mode) silicon diode, spectral range 200-1000 nm emission filter wheel w/ 8 positions (Ø 25,4 mm) performance: luminescence spectral range 340 - 650 nm detection limit for ATP <6 amol/well detection limit for Firefly Luciferase <3 zmol/well fluorescence (top) spectral range 340 - 680 nm detection limit for Fluorescein <0.3 fmol/well (384) absorbance spectral range 340 - 700 nm 0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm) precision <0,6 % cv (@ 2.5 OD) accuracy <2 % cv (@ 2.5 OD) Total Amount Net BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 2 / 11 – Dec 20, 2009 Model and Description Total Amount Net MikroWin Lite software instrument control definition of reading times and injection volumes set-up of plate layout and selection of plate format selection and definition of special reading sequences like e.g. dual ratio readings, short term and long term kinetics control of priming and cleaning sequences data evaluation free plate layout (samples, standards, controls) assignment of sample names input of dilution factors qualitative cut-off determination w/ up to 3 categories kinetics (delta values, min/max values, Vmax, AUCs, time values) combination of different evaluations free input of user defined formula calculation in up to 5 matrices results & export display in multi-matrix format or in list format graphic display of data integrated statistical functions (e.g. average, standard deviation) matrix or list format of all raw data and calculated data on demand or automatically export as file (LAN connection possible) or to printer The operation of this system requires a computer system LB 941 TriStar Multimode Reader Order No. 49178-10 LB 941 TriStar T Multimode Reader platform for these reading technologies: absorption flash luminescence glow luminescence colour luminescence (Chroma-Glo™) BRET (Bioluminescence Resonance Energy Transfer) fluorescence intensity top reading FRET (Fluorescence Resonance Energy Transfer) optimum performance and sensitivity due to dedicated optical system for each reading technology (DOPS) manual selection of optical path clear and opaque 96 and 384 well microplates (maximum dimensions w x l 86.0 mm x 128.2 mm; H 15 +/- 1 mm) up to 4 additional automatic injectors w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing injection into 384 well plates plate shaking: 3 modes (linear, orbital and double orbital), € BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 3 / 11 – Dec 20, 2009 Model and Description Total Amount Net 3 velocities well scanning w/ up to 10,000 individual reading points per well reading time per well adjustable via software: between 0.05 and 600 sec temperature control user defined between 5 °C above RT and 42 °C incl. Peltier cooling unit for stabilisation of photocathode excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h filter slide w/ 5 positions (Ø 15 mm) detection unit: photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting mode) silicon diode, spectral range 200-1000 nm emission filter wheel w/ 8 positions (Ø 25,4 mm) performance: luminescence spectral range 340 - 650 nm detection limit for ATP <6 amol/well detection limit for Firefly Luciferase <3 zmol/well fluorescence (top) spectral range 340 - 680 nm detection limit for Fluorescein <0.3 fmol/well (384) absorbance spectral range 340 - 700 nm 0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm) precision <0,6 % cv (@ 2.5 OD) accuracy <2 % cv (@ 2.5 OD) MikroWin Lite software instrument control definition of reading times and injection volumes set-up of plate layout and selection of plate format selection and definition of special reading sequences like e.g. dual ratio readings, short term and long term kinetics control of priming and cleaning sequences data evaluation free plate layout (samples, standards, controls) assignment of sample names input of dilution factors qualitative cut-off determination w/ up to 3 categories kinetics (delta values, min/max values, Vmax, AUCs, time values) combination of different evaluations free input of user defined formula calculation in up to 5 matrices results & export display in multi-matrix format or in list format graphic display of data integrated statistical functions (e.g. average, standard deviation) BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 4 / 11 – Dec 20, 2009 Model and Description Total Amount Net matrix or list format of all raw data and calculated data on demand or automatically export as file (LAN connection possible) or to printer The operation of this system requires a computer system LB 941 TriStar T Multimode Reader Order No. 49178-15 € LB 941 TriStar i Multimode Reader platform for these reading technologies: absorption flash luminescence glow luminescence colour luminescence (Chroma-Glo™) BRET (Bioluminescence Resonance Energy Transfer) fluorescence intensity top reading FRET (Fluorescence Resonance Energy Transfer) optimum performance and sensitivity due to dedicated optical system for each reading technology (DOPS) manual selection of optical path clear and opaque 96 and 384 well microplates (maximum dimensions w x l 86.0 mm x 128.2 mm; H 15 +/- 1 mm) 2 automatic injectors w/ user adjustable volumes (10 – 100 µl) in luminescence & fluorescence reading positions (#3 and #4) JET Injection for fast and complete reagent mixing injection into 384 well plates up to 2 additional automatic injectors w/ user adjustable volumes (10 – 100 µl) with wash&prime plate plate shaking: 3 modes (linear, orbital and double orbital), 3 velocities well scanning w/ up to 10,000 individual reading points per well reading time per well adjustable via software: between 0.05 and 600 sec excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h filter slide w/ 5 positions (Ø 15 mm) detection unit: photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting mode) silicon diode, spectral range 200-1000 nm emission filter wheel w/ 8 positions (Ø 25,4 mm) BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 5 / 11 – Dec 20, 2009 Model and Description Total Amount Net performance: luminescence spectral range 340 - 650 nm detection limit for ATP <6 amol/well detection limit for Firefly Luciferase <3 zmol/well fluorescence (top) spectral range 340 - 680 nm detection limit for Fluorescein <0.3 fmol/well (384) absorbance spectral range 340 - 700 nm 0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm) precision <0,6 % cv (@ 2.5 OD) accuracy <2 % cv (@ 2.5 OD) MikroWin Lite software instrument control definition of reading times and injection volumes set-up of plate layout and selection of plate format selection and definition of special reading sequences like e.g. dual ratio readings, short term and long term kinetics control of priming and cleaning sequences data evaluation free plate layout (samples, standards, controls) assignment of sample names input of dilution factors qualitative cut-off determination w/ up to 3 categories kinetics (delta values, min/max values, Vmax, AUCs, time values) combination of different evaluations free input of user defined formula calculation in up to 5 matrices results & export display in multi-matrix format or in list format graphic display of data integrated statistical functions (e.g. average, standard deviation) matrix or list format of all raw data and calculated data on demand or automatically export as file (LAN connection possible) or to printer The operation of this system requires a computer system LB 941 TriStar i Multimode Reader Order No. 49178-20 LB 941 TriStar Ti Multimode Reader platform for these reading technologies: absorption flash luminescence glow luminescence colour luminescence (Chroma-Glo™) BRET (Bioluminescence Resonance Energy Transfer) fluorescence intensity top reading FRET (Fluorescence Resonance Energy Transfer) € BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 6 / 11 – Dec 20, 2009 Model and Description Total Amount Net optimum performance and sensitivity due to dedicated optical system for each reading technology (DOPS) manual selection of optical path clear and opaque 96 and 384 well microplates (maximum dimensions w x l 86.0 mm x 128.2 mm; H 15 +/- 1 mm) 2 automatic injectors w/ user adjustable volumes (10 – 100 µl) in luminescence & fluorescence reading positions (#3 and #4) JET Injection for fast and complete reagent mixing injection into 384 well plates up to 2 additional automatic injectors w/ user adjustable volumes (10 – 100 µl) with wash&prime plate plate shaking: 3 modes (linear, orbital and double orbital), 3 velocities well scanning w/ up to 10,000 individual reading points per well reading time per well adjustable via software: between 0.05 and 600 sec temperature control user defined between 5 °C above RT and 42 °C incl. Peltier cooling unit for stabilisation of photocathode excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h filter slide w/ 5 positions (Ø 15 mm) detection unit: photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting mode) silicon diode, spectral range 200-1000 nm emission filter wheel w/ 8 positions (Ø 25,4 mm) performance: luminescence spectral range 340 - 650 nm detection limit for ATP <6 amol/well detection limit for Firefly Luciferase <3 zmol/well fluorescence (top) spectral range 340 - 680 nm detection limit for Fluorescein <0.3 fmol/well (384) absorbance spectral range 340 - 700 nm 0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm) precision <0,6 % cv (@ 2.5 OD) accuracy <2 % cv (@ 2.5 OD) MikroWin Lite software instrument control definition of reading times and injection volumes BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 7 / 11 – Dec 20, 2009 Model and Description Total Amount Net set-up of plate layout and selection of plate format selection and definition of special reading sequences like e.g. dual ratio readings, short term and long term kinetics control of priming and cleaning sequences data evaluation free plate layout (samples, standards, controls) assignment of sample names input of dilution factors qualitative cut-off determination w/ up to 3 categories kinetics (delta values, min/max values, Vmax, AUCs, time values) combination of different evaluations free input of user defined formula calculation in up to 5 matrices results & export display in multi-matrix format or in list format graphic display of data integrated statistical functions (e.g. average, standard deviation) matrix or list format of all raw data and calculated data on demand or automatically export as file (LAN connection possible) or to printer The operation of this system requires a computer system LB 941 TriStar Ti Multimode Reader Order No. 49178-21 € LB 941 TriStar vTi Multimode Reader platform for these reading technologies: absorption flash luminescence glow luminescence colour luminescence (Chroma-Glo™) BRET (Bioluminescence Resonance Energy Transfer) fluorescence intensity top reading FRET (Fluorescence Resonance Energy Transfer) optimum performance and sensitivity due to dedicated optical system for each reading technology (DOPS) manual selection of optical path clear and opaque microplates (6, 12, 24, 48, 96 and 384 well) (maximum dimensions w x l 86.0 mm x 128.2 mm; H 14 mm - 20 mm) 2 automatic injectors w/ user adjustable volumes (10 – 100 µl) in luminescence & fluorescence reading positions (#3 and #4) JET Injection for fast and complete reagent mixing injection into 384 well plates up to 2 additional automatic injectors w/ user adjustable volumes (10 – 100 µl) with wash&prime plate plate shaking: 3 modes (linear, orbital and double orbital), 3 velocities BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 8 / 11 – Dec 20, 2009 Model and Description Total Amount Net well scanning w/ up to 10,000 individual reading points per well reading time per well adjustable via software: between 0.05 and 600 sec temperature control user defined between 5 °C above RT and 42 °C incl. Peltier cooling unit for stabilisation of photocathode excitation unit: Tungsten halogen lamp, 75 W, spectral range 340–700 nm, typical life time >300 h filter slide w/ 5 positions (Ø 15 mm) detection unit: photomultiplier tube (low noise photocathode, high cathode sensitivity, digital photon counting mode) silicon diode, spectral range 200-1000 nm emission filter wheel w/ 8 positions (Ø 25,4 mm) performance: luminescence spectral range 340 - 650 nm detection limit for ATP <6 amol/well detection limit for Firefly Luciferase <3 zmol/well fluorescence (top) spectral range 340 - 680 nm detection limit for Fluorescein <0.3 fmol/well (384) absorbance spectral range 340 - 700 nm 0 - 3.5 OD @ 400 - 700 nm (0 - 3 OD @ 340 nm) precision <0,6 % cv (@ 2.5 OD) accuracy <2 % cv (@ 2.5 OD) MikroWin Lite software instrument control definition of reading times and injection volumes set-up of plate layout and selection of plate format selection and definition of special reading sequences like e.g. dual ratio readings, short term and long term kinetics control of priming and cleaning sequences data evaluation free plate layout (samples, standards, controls) assignment of sample names input of dilution factors qualitative cut-off determination w/ up to 3 categories kinetics (delta values, min/max values, Vmax, AUCs, time values) combination of different evaluations free input of user defined formula calculation in up to 5 matrices results & export display in multi-matrix format or in list format graphic display of data integrated statistical functions (e.g. average, standard deviation) matrix or list format of all raw data and calculated data BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 page 9 / 11 – Dec 20, 2009 Model and Description Total Amount Net on demand or automatically export as file (LAN connection possible) or to printer The operation of this system requires a computer system LB 941 TriStar vTi Multimode Reader Order No. 49178-25 € Options and accessories Injector #1 pre-reading position luminescence w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing injection into 384 well plates Order No.: 37772-11 € Injector #2 reading position luminescence w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing injection into 384 well plates Order No.: 37772-12 € Injector #3 reading position luminescence w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing injection into 384 well plates Order No.: 37772-13 € Injector #4 reading position fluorescence w/ user adjustable volumes (10 – 100 µl) JET Injection for fast and complete reagent mixing Order No.: 37772-14 € BRET package selected filter pairs for BRET and BRTE² assays 400 nm (Deep Blue C™), 515 nm (GFP²), 480 nm (Coelenterazin) and 530 nm (YFP) Order No.: 39350 BRET1 “high efficiency” package € BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 Model and Description page 10 / 11 – Dec 20, 2009 Total Amount Net Selected BRET filter pairs 480 nm (Coelenterazin) and 540 nm (YFP) with transmission characteristics to double signal for extremely weak experessions Order No.: 53431 € BRET² “high efficiency” package Selected BRET² filter pairs 410 nm (Deep Blue C™) and 515 nm (GFP²) with transmission characteristics to double signal for extremely weak experessions Order No.: 53432 € Additional filter slide “B” for excitation filters 5 positions for filters with ø 15 mm Order No.: 41537 € Additional filter slide “B” for excitation filters 5 positions for filters with ø 15 mm equipped with absorption filters 405 nm, 450 nm, 490 nm, 620 nm Order No.: 41538 € Additional filter slide “C” for excitation filters 5 positions for filters with ø 15 mm Order No. 52539 € Additional filter wheel “B” for emission filters 8 positions for filters with ø 25 mm Order No.: 55384 Additional filter wheel “C” € BERTHOLD-BIO Item Qty. Quotation Texts TriStar LB 941 Model and Description page 11 / 11 – Dec 20, 2009 Total Amount Net for emission filters 8 positions for filters with ø 25 mm Order No.: 55385 € Chroma-Glo™ package selected filter pair 510 nm and 610 nm Order No.: 43544 € Robot Integration Module software patch for control and communication with robot software for integration of the TriStar into laboratory automation systems documentation of the communication language Order No.: 39354sp € Prime&Wash Plate to receive excess liquids from prime and wash activities Order No.: 55018 € Adapter Frame IWAKI clear for unlidded clear Iwaki plates to be used with TriStar vTi (49178-25) Order No.: 54667 € Adapter Frame Falcon 353075 for lidded BD Falcon 353075 to be used with TriStar vTi (49178-25) Order No.: 55616 €
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