Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Student Author Name:
Team Members Name(s):
Student Author ID #:
Team Members ID #’(s):
Lab Assignment #:
Team Name: Ditz
Lab Assignment Title: Blood Glucose
Date: 2017-04-19
Background
▪
▪
Before this lab, we went in with the understanding our body responds to the consummation of carbohydrates by releasing
insulin and glucagon.
This investigation may add to our knowledge by providing an understanding of the body’s physiological response to an
intake of carbohydrates.
Purpose / Objective(s):
▪
▪
▪
To make observations about our blood glucose levels throughout the 40-minute lab
To record the nature of our blood glucose levels throughout the 40-minute lab
To make calculations about the relationships between the data collected
Hypothesis:
▪
▪
If the subject is given a dose containing sugar, their blood glucose level should reach to normal blood glucose level within
40 minutes of ingesting the dose.
Blood glucose levels will rise after consuming a dose of sugar
Subject(s):
Table #1: Subject characteristics
The following table characterizes the subjects in this study:
Between this group of males and females, age ranged from 18 to 60 and BMI ranged from 19 to 30.
M= Males and F= Females.
MK
VN
MH
BR
MA
Gender
F
F
F
M
F
Age
20
20
23
60
19
BMI
19
22
27
30
28
EH
M
18
24
Page 1 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Instruments:
▪
▪
Lancets
Lancing devices
Test strips
Blood glucose test meter
▪
Materials:
▪
Tissue Paper
Antibacterial hand sanitizer
Sugar
Candies
▪
▪
▪
▪
▪
Instruments / Procedures:
Subjects
Instruments/Materials
▪
All Subjects
▪
▪
▪
▪
▪
▪
▪
▪
Antibacterial hand sanitizer
Tissue Paper
Lancets Attachment 1
Lancing device Attachment
2
Test strips Attachment 1
Blood glucose test meter
Attachment 2
Sugar
Candies
Procedures and Methods
1. Record age, and BMI into the Blood
Glucose data tables
2. Prepared a pinky finger to test blood
glucose by cleaning with antibacterial hand
sanitizer on tissue paper and wiped dry.
3. Prepared a lancing device with a new
lancet.
4. Used the lancing device on the prepared
finger.
5. Used the blood glucose test meter and new
test strip to record our blood glucose level.
6. Each subject had a variation of either
placebo, sugar, or candies.
7. Right after the subject ingested the dose,
repeated 2-6 to record blood glucose.
8. Waited 20 minutes before repeating steps 26 again to record 20-minute post dose blood
glucose.
9. Waited 20 more minutes before repeating
steps 2-6 again to record 40-minute post
dose blood glucose.
Page 2 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Results / Data:
Graphs
▪
Attachment 3
o This graph displays the blood glucose levels for all subjects. All subjects displayed a peak in their blood glucose
levels at 20-minutes post dose then reached within normal blood glucose levels.
▪
Attachment 4
o This graph displays the blood glucose of MK, the subject who ingested placebo, and VN, the subject who
ingested 27g of candy. The subject VN had the largest sugar dose. Using MK as a baseline, initial starts out
the same however after 20-minute dose VN’s blood glucose jumped 33 mg / dL while MK’s only rose 4 mg / dL.
At 40-minutes post dose VN’s post dose continued to climbed.
▪
Attachment 5
o This graph displays the conditions for MK and MH. The subject MK took the placebo and the subject MH took 10g
of sugar. She had the largest peak blood glucose level at 150 mg / dL which is considered pretty high for the normal
person.
▪
Attachment 6
o This graph displays a decrease It is unknown why subject MA’s blood glucose decreased over the 40-minute
lab. This subject ingested sugar and candy yet their blood glucose managed to remain within normal
levels both 20-minutes and 40-minutes post dose.
▪
Attachment 7
o This graph displays the initial blood glucose for all subjects. It expressed a baseline for all subjects that
indicated all subjects’ blood glucose levels were within normal levels, 70-100 mg/dL. These subjects are
likely not diabetic nor at risk of being diabetic.
▪
Attachment 8
o This graph displays the blood glucose of dose of H20 vs dose of candy. Since the dose of candy was potentially the
highest dose of sugar at 27g this should have displayed the highest blood glucose level after 20-minutes post dose.
However this wasn’t the case, subject MH displayed the highest blood glucose at 150 mg / dL with her sugar intake
of 10g.
▪
Attachment 9
o This graph displays the correlation between blood glucose and BMI. There doesn’t seem to be a correlation
between blood glucose and BMI. Generally type two diabetes has a correlation with higher BMI however
my findings show no such correlation.
Analysis / Discussion:
▪
▪
It was determined that after peak over time, blood glucose will decrease to within normal level if the subject is not diabetic.
Our hypothesis was validated; all of the subjects displayed a blood glucose within normal level after 40 minutes post dose.
The chart in Attachment 6 displays The Blood Glucose Levels Over Time for All Subjects.
Conclusions / Further Considerations:
▪
▪
▪
▪
All subjects blood glucose levels vary due to the dose of sugar taken.
There seems to be a correlation between blood glucose and dose of sugar since the more sugar the body takes in, the more
insulin the pancreas has to make and distribute.
This understanding allows us to further expand our knowledge by providing new perspective of a part of the endocrine
system and how sugars affect the human body.
A question this lab has proposed is; if we were to expand our sample size and include diabetics, how would this affect our
Page 3 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
▪
▪
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
results?
Some research that could be done following this experiment is to research; how individuals diagnosed with diabetes, have to
compensate for a body that does not produce insulin.
Another area of investigation to help further our understanding would be to explore artificial insulin and how that helps
diabetics manage their blood glucose levels.
Tables:
Table #2 – Blood Glucose Data
This table displays the blood glucose levels for all the subjects in this experiment.
MK
VN
MH
BR
Glucose
H20
Candy (27g)
10g Sugar
10g Sugar
Dose
BG Initial
86
86
102
107
BG 20 Min
90
119
150
134
Post Dose
BG 40 Min
94
123
120
126
Post Dose
BG Mean
90
109
124
122
MA
10g Sugar +
Candy
112
101
EH
10g Sugar +
Candy
77
103
100
78
104
86
Attachments:
Subjects: MK=1, VN=2, MH=3, BR=4, MA=5, EH=6.
Attachment 1 – Test Strips and Lancets
On the right are three test strips and on the left, are lancets. All six of these instruments were used during the lab to
keep the area sanitary.
Page 4 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Attachment 2 – Blood Glucose Meter and Lancing Device
On the right is a blood glucose meter, which when paired with a test strip measures blood glucose level. On the left is
a lancing device, when prepared with a lancet pricks the right amount of blood to be tested.
Attachment 3 – The Blood Glucose Levels for All Subjects
For all subjects blood glucose is dependent on sugar intake.
Page 5 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Attachment 4 – The Blood Glucose of Dose H20 vs Blood Glucose of Dose Candy (27g)
Subject VN had the highest sugar dose and subject MK was the baseline for blood glucose levels.
Attachment 5 – Conditions for Subjects MK and MH
Subject MH’s condition is compared to the average conditions for all subjects and MH displayed an interesting peak
20-minutes post dose.
Page 6 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Attachment 6 – The Blood Glucose Levels Over Time for All Subjects
All subjects that took a dose of sugar displayed a peak in blood glucose level at 20-minutes post dose except subject
MA.
Attachment 7 – The Initial Blood Glucose for All Subjects
This is the baseline for all subjects indicates that for all subjects’ blood glucose levels started out all over the board
however they were all within normal levels, 70-100 mg/dL.
Page 7 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Attachment 8 – Blood Glucose 20 Minutes Post Dose vs Sugar Dose
There is a strong correlation between blood glucose and the dose of sugar ingested; The higher the sugar dose, the
higher the peak was post 20 minutes.
Attachment 9 – Blood Glucose 20 Minutes Post Dose vs BMI
There’s not much of a correlation between blood glucose and BMI.
Page 8 of 9
Document1
Biology 105 – Human Biology
Laboratory Report
Session 2017:
Sections:
Class Location:
Days / Time:
Instructor:
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
T 9:30 AM – 12:20 PM
LAB
RIDDELL
Spring
70651 4 Units
2210 / 2030.
T Th 8:00 AM – 9:15 AM LEC
Th 9:30 AM – 12:20 PM LAB
RIDDELL
Page 9 of 9
Document1