Multiple cloning site (MCS) of OriGene cloning vectors
pCMV6-Entry (TrueORF vector)
Vector Primer VP1.5 >
TTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTAC
T7 promoter
Sac I
(Not for sequencing
EcoR I
GGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGT
Kozac
BamH I Kpn I
Sgf I
Asc I
Hind III
Rsr II Mlu I
CGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGCTAGCGGACCGACGCGT
Not I Xho I
Myc-tag
EcoR V
DDK tag
ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGATGACGACGATAAG
Pme I
Fse I
GTTTAAACGGCCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGG
CCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAA
GGGTGGTCGGAACAGGATTA
< Vector Primer XL39
pCMV6-AC-GFP (TrueORF vector)
Vector Primer VP1.5 >
TTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTAC
T7 promoter
Sac I
(Not for sequencing)
EcoR I
GGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGT
Kozac
BamH I Kpn I
Sgf I
Asc I
Hind III
Rsr II Mlu I
CGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGACCGACGCGT
Not I Xho I
Pme I
ACGCGGCCGCTCGAG---tGFP---GTTTAAACGGCCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGT
GACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAA
GGGTGGTCGGAACAGGATTA
< Vector Primer XL39
pCMV6-XL4, XL5 and pCMV6-Neo (TrueClone vectors)
Vector Primer VP1.5 >
TTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACG
T7 promoter
Sac I
(Not for sequencing)
Not I EcoR I
GTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATT
Xba I
Not I
Sma I
C----TrueClone Insert----CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACATGTGATCCCGGGT
GGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAA
GGGTGGTCGGAACAGGATTA
< Vector Primer XL39
pCMV6-XL6 (TrueClone vector)
Vector Primer VP1.5 >
TTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACG
SP6 Promoter >
Sac I (not for sequencing)
Not I EcoR I
GTGGGAGGTCTATATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATT
Xba I
Not I
Sma I
C----TrueClone Insert----CTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCCTGAACATGTGATCCCGGGT
GGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAA
GGGTGGTCGGAACAGGATTA
< Vector Primer XL39
pCMV6-AC (TrueClone vector)
Vector Primer VP1.5 >
TTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCCCGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTAC
T7 promoter
Sac I
(Not for sequencing)
EcoR I
GGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGT
Kozac
BamH I Kpn I
Sgf I
Asc I
Hind III
Rsr II Mlu I
CGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGACCGACGCGT
Not I
Xho I Pme I
Fse I
TAAGCGGCCGCACTCGAGGTTTAAACGGCCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCCCT
CCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAA
GGGTGGTCGGAACAGGATTA
< Vector Primer XL39