The next generation of optical imagers
3D/4D
high resolution
multispectral
analysis
In Actio
x-ray
Real-Time bioluminescence
and fluorescence Imaging systems
A uniquely modular instrument
for detecting, localizing, quantifying dynamic
bioluminescence or fluorescence signals
both in vivo and in vitro.
Bioluminescence & Fluorescence Imaging
Unmatched Performance & accuracy
Main Applications of
the PhotonIMAGERtm
Biodistribution
Cancer Research
Gene Expression
Infectious desease
Neuroscience
Pharmacokinetics
Stem Cell Research
Real-time imaging capability
The importance of real-time signal acquisition
Bioluminescence signal from mice following subcutaneous injection of
Mesenchymal stem cells expressing Luciferase
Real-Time kinetic curves of the three different
Bioluminescence acquisition of three mice
mice during the same acquisition
at the same time
mouse 1
mouse 2
mouse 3
A pre-determined standard
kinetic curve
is often used as a reference
for determining the acquisition time needed for bioluminescence imaging.
This takes no account of the
differences in signal kinetics
obtained in different animals.
Images taken
every 23ms
Mouse 1
measured
at the signal
plateau of
the Real-Time
kinetic curve of
mouse 1
Mouse 1
measured
at the signal
plateau
of the standard
kinetic curve.
The PhotonIMAGERtm
is the only system which provides precise
control over the time-zone selected for the
measurement of each ROI,
The signal peak
has been missed
both during and following signal acquisition
One modular system for all applications
An upgradeable system with
state-of-the-art technology development
In Actio® module
Macrolens module
for kinetic imaging of freely-moving animals
for higher resolution optical imaging
4View® module
TomoFluo Module
for simultaneous multi-angle acquisition
for accurate 3D fluoescence imaging
3D/4D module
Multispectral module
for 3D and 4D kinetic rendering
of 4-View imaging
for multispectral imaging
X-Ray / StereoCT
for accurate anatomic localization and quantification of signals
Luciferase expressing Hep3B tumor cells injected subcutaneously and in the liver of a mouse
Volumetric bioluminescent signal
reconstruction from the
3D module data using the
M3Vision Analysis Software
Real-Time bioluminescent
Anatomic localization of
signal acquisition
bone metastasis using the
using the 4-View module
X-Ray module
User Friendly
Technical Specifications
PhotonIMAGERtm
RT
PhotonIMAGERtm
Optima
Camera
Sensor
Intensified CCD Camera (18mm)
Intensified CCD Camera (25mm)
Objective Lens
24mm, f/1.4 - 22
50mm, f/1.2 - 16; 35 mm, f/1.4 - 22 (on request)
Operating Temperature
-25°C
-25°C
Detection Spectral Range
370 - 900nm
370 - 900nm
Temporal Resolution
23ms
54 ms (default), 27 or 13ms on request
Minimum Detectable Radiance
<80 photons/s/sr/cm² without binning
<40 photons/s/sr/cm² without binning
Binning
Not needed
Not needed
Dynamic Range
>6.0 orders of magnitude
>6.0 orders of magnitude
CCD Read Noise
No
No
Dark Current Noise
25 e-/s/cm²
25 e-/s/cm²
Field of View (FOV)
Min: 8 x 6cm (standard system)
Min: 3.4 x 2.8mm (with Macrolens)
Min: 8 x 6cm (standard system)
Min: 3.4 x 2.8mm (with Macrolens)
Max: 25,5 x 18cm
Max: 22 x 16.5cm (50mm lens)
32 x 24cm (35mm lens)
110µm (standard system)
5µm (with Macrolens)
45µm (standard system)
2µm (with Macrolens)
Source
150W Halogen lamp
150W Halogen lamp
Fluorescence
Selectable multi-wavelength
Selectable multi-wavelength
Excitation Range
400 - 730 nm or 500 - 750nm
400 - 800 nm
Emission Filters
6 Filters
15 Filters
Performance
Minimum Image Pixel Resolution
Illumination
Filters
Autofluorescence Substraction
Multilabelling Capability
Spectral Unmixing
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Animal Management
Gas Anesthesia
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Heated Stage
25°C - 45°C
25°C - 45°C
Imaging Chamber Size
25 x 26 x 38cm (WxDxH)
50 x 40 x 70cm (WxDxH)
Modules
3D Reconstruction Software
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2D X-Ray Analysis and Registration
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22 x 11cm
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Kinetic Imaging
Simultaneous Multiple Views
InActio Analysis & Registration
X-Ray field of view
Atlas overview & organ quantification
StereoCT
TomoFluo
System Requirements
Operating System
Windows 7
Windows 7
Power Comsumption
1KW @ 150 or 230V
1KW @ 150 or 230V
Dimensions
80 x 58 x 106cm (WxDxH)
87 x 60 x 142cm (WxDxH)
Biospace Lab SA, France
13, rue Georges Auric
75019 Paris France
t + 33 (0)1 44 52 88 10
f + 33 (0)1 44 52 88 39
[email protected]
www.biospacelab.com
Biospace Lab, Inc., USA
185 Alewife Parkway
#410Cambridge, MA
02138, USA
t + 1 201 785 4032
[email protected]