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Production of Engineered long-life and
male-sterile Pelargonium plants
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Key Words
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Pelargonium zonale and Pelargonium peltatum
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pSAG12 and PsEND1 promoter
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Engineered long-lived and male sterile plants
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ipt gene: expressed in long-living plants
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Barnase: gene expressed in pollen free plants
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Agrobacterium tumefaciens
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Delayed senescence
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Anther ablation: abnormalities in anther production
Background
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Flowering plant
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Grown in Europe since 17th century
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More than 200 species of Pelargonium
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--Cultivars: P. zonale
and P. peltatum is the most
popular.
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Species of Pelargonium are considered important
in:
•
Market of ornamental plants
•
Perfume industry
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Background
Pelargonium Breeds
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Pelargonium breeders produce
new flower colors, shapes and
market quality plants via gene
transfer
Long-living plants
Allergen-Free house plants
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Plant Manufacturers
-Agrobacterium
tumefaciens
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Crown Gall Disease
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Important in gene transfer
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Regeneration of plants
In vitro regeneration system
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Pelargonium zonale explant used as a starting
materials.
-P. zonale
– organogenesis
- shoots
-Two
new traits in P. zonale
-Long-life
-
plants
Male sterility
-Promoter
used:
-pSAG12
– Senescence-specific gene promoter
-PsEND1
promoter – Anther specific promoter
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Plant senescence
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Study of aging in plants
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pSAG12 promoter fuses with ipt gene which has an ipt enzyme
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Produce more cytokinin
Delayed Senescence
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Influence of pSAG12:ipt
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Male Sterile Plants
-Why
are they important?
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eliminates pollen allergens
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Prevents gene flow of GM material and related plant species.
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Elimination of pollen allergens
Expression
of barnase
gene
- Anther –
specific
PsEND1
promoter
-Specific
ablation in
plants
-Anther tissues are necessary
- No pollen grains in ablated anthers
- Barnase destroys cells for structural tissues
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Pelargonium zonale explant
Soak it in Agrobacterium
- Plasmid with T-DNA region that
contains
Ipt and Barnase gene under the
control of their promoters
DNA  chromosome
= tumors
Place into Media with
Hormones
- Roots
- Shoots
Plasmid leaves bacteria, goes into
plant cells
Transfer to a pot to fully grow
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RESULTS
pSAG12::ipt transgenic
plants
Delayed leaf senscence
Increased branching
Smaller flowers
Intense coloration
Transgenic lines with
PsEND1::barnase
No pollen grains in
anther structures
References
BioMed Central Limited (2012, August 29). No more sneezing: Allergen-free house plants.
ScienceDaily. Retrieved November 18, 2012, from http://www.sciencedaily.com
/releases/2012/08/120831083315.htm
BioMed Central Limited (31 August 2012). Production of engineered long-life and male sterile
Pelargonium plants. [Research Article] Available at: http://www.biomedcentral.com/14712229/12/156 . [Last Accessed 18 November 2012].
Biomedcentral.com (2012) www.biomedcentral.com - Figure. [online] Available at:
http://www.biomedcentral.com/1471-2229/12/156/figure/F5 [Accessed: 27 Nov 2012].
Maria Lis-Balchin, (2007). Geranium and Pelargonium: The Genera Geranium and Pelargonium. 1st
ed. New York: Taylor & Francis.
Mary Spink. Growing Pelargoniums. [ONLINE] Available at: :
http://www.swanlandnurseries.co.uk/culturalnotes.asp. [Last Accessed 18 November 2012].
Scielo.cl (2007) Electronic Journal of Biotechnology - Influence of 2,4-D and BAP on callus growth and
the subsequent regeneration of somatic embryos in long-term cultures of Pelargonium x domesticum
cv. Madame Layal. [online] Available at:
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007 [Accessed: 18
Nov 2012].