WP 4 – Valorisation of algal biomass
Second Consortium Meeting
13-14 June
Stockholm, Sweden
H2020-Water1b-2015
This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement no 689785
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Introduction: WP4
WP4: Valorisation of Algae Biomass
H2020-Water1b-2015
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Biomass Harvesting:
Biomass Refinement:
Animal Feed:
Coatings and Adhesives:
Fillers and pastes:
IBET
Extractis
PMIX
INSP
INSTM/POLIMI
Milestones and Deliverables
Milestones and Deliverables
H2020-Water1b-2015
Milestones
• MS5 Optimal pre-concentration by membrane ultrafiltration and low shear centrifugation iBET M16
sept-17
• MS6 Choice of the best biomass refinement process Extr M16 sept-17
• MS7 Decision on scale up demonstration activities for polymer compounding, pastes and coatings
INSTM M23 apr-18
• MS8 Valorisation activities: polymer compounding, pastes, coatings and animal feed feedstocks
PMIX M36 may-19
Deliverables
• D4.1 Systems design for harvesting and refinement and algae characterisation iBET M14 jul-17
• D4.2 Biomass valorisation report – feasibility Extr M20 jan-18
• D4.3 Biomass valorisation progress report – first generation products INSP M28 sept-18
• D4.4 Final biomass valorisation report (animal feed, resins and residues) INSTM M36 may-19
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iBET
Extractis
Task 4. 2 Biomass refinement and extraction
Objectives
 Efficiency of the pre-treatment (DM in the sludge around 20%)
 Fractionate algae biomass into three fractions consisting in respectively oily phase, aqueous phase
and sludge with, at least, 80% purity of the oily phase and protein content above 65% of the protein
fraction.
 Purity of beta-carotene (≥ 80%) and digestibility of the protein phase equivalent to soy concentrate.
Deliverables
 D4.2 Biomass valorisation report - Feasibility (M20)
H2020-Water1b-2015
Milestones M16
 Choice of the best biomass refinement process
 Verification :
- 80% purity of the oily phase
- Protein content above 65%
- Purity of beta-carotene (≥ 80%).
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Task 4. 2 Biomass refinement and extraction
Description of actions and progress
Q1
Task 4.2
Year 1
Q2
Q3
Q4
Q1
Year 2
Q2
Q3
Q4
Q4
Biomass refinement and extraction
Description of actions
Task 4.2 Pretreatment
Samples from DEMO SITES to be received
Progress
Actions realized
Intensify the best classical conditions /
ultrasounds (design of experiences to test various
parameters at lab scale) / Compare to HPH.
Samples received ( Wet algae from ARAVA and KOTO, Lyophilized algae from ARCHIMEDE)
Bibliography shows that: - some pretreatments can be used alone (HPH and US) - chemical treatment
could affect the nature of the proteins 1 enzyme tested depending on several parameters: VISCOZYME.
Not relevant regarding the results obtained.US and HPH tested alone (according to experimental
designs). Not relevant.
Realization of the proteins solubility curves in order to determinate the optimal pH of solubilization
and precipitation for each algae. EXTRACTIS is also testing HPH and ultrasounds followed by
chemical treatment. Samples (residues and proteins) sent to INSPIRALIA and POLIMI.
Scale up the conditions on a micropilot equipment.
It will be done later once the best candidate will be determined
Characterizations of the initial and final products
(dry matter, proteins, ash, sugars…). Measurement
of the enzymatic activity. HPH
DM, ash and proteins are measured on each supernatant and pellet. Other characterizations are
done on relevant samples (Free and total sugars, lipids) .
Bibliographical survey / Identify the classical
conditions to be tested (acidic and enzymatic
conditions)
Task 4.2b: Three- stage separation
Separation with a three phases decanter
H2020-Water1b-2015
Q1
Year 3
Q2
Q3
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At the moment, two phases are obtained, not three.
Task 4.2 Biomass refinement and extraction
H2020-Water1b-2015
Last results
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Task 4.2 Biomass refinement and extraction
Sample
Algae
Characterizations
Yields
7
Archimede Spirulina (HPH +pH11)
DM : 98,9%
Ash (%/DM) :22.6
Proteins (%/DM) : 35.7
ND
8
Archimede Spirulina (pH4)
DM : 99,6%
Ash (%/DM) : 3.4
Proteins (%/DM) : 72.2
Solubilisation yield (Prot supernatant 1 / prot algae)= 59%
Global yield (prot powder/prot Algae) = 26%
9
Archimede Tetraselmis (HPH+pH11) DM : 99,9%
Ash (%/DM) : 16.9
Proteins (%/DM) : 20.6
ND
10
Archimede Tetraselmis (pH4)
Solubilisation yield (Prot supernatant 1 / prot algae)= ND
Global yield (prot powder/prot Algae) = 40%
DM : 99,5%
Ash (%/DM) : 4.21
Proteins (%/DM) : 56.2
H2020-Water1b-2015
 2 phases, no lipidic phase
 Last characterizations in progress : free and total sugars, lipids
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Task 4.2 Biomass refinement and extraction
Open issues
Regarding the quantities of lipids requested by PRODUMIX (6kg of lipids which correspond to 300 kg of
biomass):
- at the moment, we are not able to extract lipids.
- Even if the combination HPH or Ultrasounds pretreatment followed by a chemical treatment is
efficient for the proteins extraction, it’s not the case for the lipids.
- Will a third approach be necessary?
- Will the demo sites be able to provide such fresh quantities to EXTRACTIS?
Timing plan
- Optimization: - Proteins solubility curves for each algae realized previously
- Determination of the best acidic and alcaline pH conditions
- Increase the purity of the proteins fraction
- Extraction of lipids by optimizing the HPH parameters (number of passes, number of stages…)
Requirements from
H2020-Water1b-2015
- DEMO SITEs (ARAVA, KOTO and ARCHIMEDE) : algae
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PMIX
INSP
INSTM
Introduction: Our role
WP4.5: High value material fillers & pastes
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H2020-Water1b-2015
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4.5a: Algae-residue characterization and
modification
4.5b: Development of algae-based
biocomposites
4.5c: Development of algae-based 3Dprinting of ceramic materials and liquid
pastes
H2020-Water1b-2015
Introduction: Chemical process
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Microscopy
H2020-Water1b-2015
TGA analysis
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Spirulina
Tetraselmis
~8.6% final weight
~17.0% final weight
Microscopy – Spirulina 5%
Pre-heat
50x
H2020-Water1b-2015
100x
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2h@ 800°C
Microscopy – Tetraselmis 5%
Pre-heat
50x
H2020-Water1b-2015
100x
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2h@ 800°C
Mechanical properties
Composition
Process
modifiers
H2020-Water1b-2015
Chemically
reactive part
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H2020-Water1b-2015
Compression Test
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Compression Test
• Empty cylinder samples
• 3D printed
15 mm
7.5 mm
H2020-Water1b-2015
25 mm
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Compression Test – Printed Pipes: time evolution
20
Compression Strength (MPa)
18
16
14
1d
12
7d
28 d
10
08
06
04
H2020-Water1b-2015
GeoP
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Sp 1
Sp 3
Sp 5
Ttr 1
Ttr 3
Ttr 5
H2020-Water1b-2015
TGA analysis
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Spirulina
Tetraselmis
~8.6% final weight
~17.0% final weight
Compression Test – Printed Pipes: heating
2h @800h
50
45
Compressive Strength (MPa)
40
35
30
7d
7d heat
25
28d
20
28d heat
15
10
05
00
H2020-Water1b-2015
GeoP
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Sp 1
Sp 3
Sp 5
Ttr 1
Ttr 3
Ttr 5
H2020-Water1b-2015
Ball milling
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Compression Test – Printed Pipes: milling
20
24h milling
18
16
Compressive Strength (MPa)
14
12
7d
10
7d mill
08
06
04
02
00
H2020-Water1b-2015
GeoP
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Sp 1
Sp 3
Sp 5
Ttr 1
Ttr 3
Ttr 5
Compression Test
H2020-Water1b-2015
•Milling 24h
•Printing
•Heating 800°C
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Compression Test – Printed Pipes: heating and
milling
45
2h @800h
24h milling
40
Compressive Strength
35
30
25
7d heat
7d heat mill
20
15
10
05
00
H2020-Water1b-2015
GeoP
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Sp 1
Sp 3
Sp 5
Ttr 1
Ttr 3
Ttr 5
Next steps
Nextsteps
• Start 4.5b: Development of algae-based biocomposites
with natural rubber and possibly other polymers
• Complete the last mechanical tests
H2020-Water1b-2015
• Validate the results with algae fractions
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Thank you for your kind attention