A) Metabolic Features: Carbon Cycling
Genome Analyzed
Protein Encoded
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Decarbonylase
Acetyl-CoA
Gene
Name or
Subunit
Scaffold(
s)
Gene(s)
AA or NT
Alignment
%ID to
M. Mahii
Ref Seq
Alignment
Length
A
263
12
AA
96%
801
Length
mahii
Ref Seq
(aa)
801
B
263
14
AA
97%
465
465
D
263
16
AA
96%
439
439
E
263
13
AA
94%
170
170
G
263
17
AA
97%
470
470
A
8
11
AA
96%
801
801
B
8
13
AA
97%
465
465
D
8
15
AA
96%
439
439
E
8
12
AA
94%
170
170
G
8
16
AA
97%
470
470
A
1109
2
AA
96%
801
801
B
445
5
AA
97%
465
465
D
445
3
AA
96%
439
439
E
445 &
1109
445
6&1
AA
95%
167
170
1
AA
97%
470
470
44
39
AA
91%
646
646
G
Part of
Metabolism
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Synthetase
Acetyl-CoA
Synthetase
Acetyl-CoA
Synthetase
Acetyl-CoA
Synthetase
Acetyl-CoA
Synthetase
Acetyl-CoA
Synthetase
Pyruvate
Phosphate
Dikinase
Pyruvate Kinase
118
23
AA
94%
490
490
2
15
AA
91%
646
646
0
AA
93%
489
490
291
170 &
171
6
AA
91%
646
646
1536
2
AA
94%
490
490
1216
5
AA
95%
880
885
271
28
AA
96%
477
477
Marcellus Day 328
Mh bin
PEP Synthetase
1358
3
AA
95%
802
802
Methanohalophilus
sp. fracturphilus
Pyruvate
Phosphate
Dikinase
Pyruvate Kinase
5
26
AA
95%
880
885
0
238
AA
96%
477
477
Methanohalophilus
sp. fracturphilus
PEP Synthetase
0
314
AA
96%
880
802
Utica Day 98
Mh bin
Pyruvate
Phosphate
Dikinase
Pyruvate Kinase
108
17
AA
95%
880
885
1314
1
AA
96%
477
477
PEP Synthetase
636
1
AA
96%
802
802
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Acetyl-CoA
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Phosphoenol
-pyruvate
Generation
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Ferredoxin
Oxidoreductase
Pyruvate
Phosphoenol
-pyruvate
Generation
Pyruvate
A
281
5
AA
97%
404
404
B
281
4
AA
98%
292
292
Generation
Pyruvate
D
281
6
AA
94%
86
87
Generation
Pyruvate
G
281
7
AA
97%
181
181
Generation
Pyruvate
A
19
18
AA
97%
404
404
Generation
Pyruvate
B
19
17
AA
98%
292
292
Generation
Pyruvate
D
19
19
AA
94%
86
87
Generation
Pyruvate
G
19
20
AA
97%
181
181
Generation
Pyruvate
A
281
5
AA
97%
404
404
Generation
Pyruvate
B
281
4
AA
98%
292
292
Generation
Pyruvate
D
281
6
AA
94%
86
87
Generation
Pyruvate
G
281
7
AA
97%
181
181
Generation
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Ferredoxin
Oxidoreductase
Pyruvate
Carboxylase
Pyruvate
Carboxylase
Pyruvate
Carboxylase
Pyruvate
Carboxylase
Pyruvate
Carboxylase
Pyruvate
Carboxylase
Citrate
Synthase
Citrate
Synthase
Citrate
Synthase
Aconitase
Pyruvate
A
S.T.
NT
93%
1497
1497
B
193 &
901
173
24
AA
95%
576
576
A
5
57
AA
97%
498
498
B
5
58
AA
95%
576
576
A
635
3
AA
97%
498
498
B
635
2
AA
95%
576
576
330
10
AA
75%
424
425**
Pyruvate ->
Oxaloacetate
Pyruvate ->
Oxaloacetate
Pyruvate ->
Oxaloacetate
Pyruvate ->
Oxaloacetate
Pyruvate ->
Oxaloacetate
Pyruvate ->
Oxaloacetate
TCA
5
94
AA
75%
424
425**
TCA
1373
1
AA
75%
424
425**
TCA
S.T.
NT
94%
2778
2778
TCA
Aconitase
1136 &
1217
7
8
AA
95%
925
925
TCA
Aconitase
622
3&4
AA
96%
925
925
TCA
Isocitrate
Dehydrogenase
Isocitrate
Dehydrogenase
Isocitrate
Dehydrogenase
Alpha
ketoglutarate
ferredoxin
oxidoreductase
330
9
AA
94%
338
338
TCA
5
93
AA
94%
338
338
TCA
1373
2
AA
94%
338
338
TCA
543
18
AA
93%
559
576
TCA
A
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Alpha
ketoglutarate
ferredoxin
oxidoreductase
Alpha
ketoglutarate
ferredoxin
oxidoreductase
Alpha
ketoglutarate
ferredoxin
oxidoreductase
Alpha
ketoglutarate
ferredoxin
oxidoreductase
Alpha
ketoglutarate
ferredoxin
oxidoreductase
Malate
Dehydrogenase
Malate
Dehydrogenase
Malate
Dehydrogenase
Fumarase
B
543
17
AA
94%
284
284
TCA
A
17
5
AA
93%
559
576
TCA
B
17
6
AA
94%
284
284
TCA
A
759
4
AA
93%
559
576
TCA
B
759
3
AA
94%
284
284
TCA
192
41
AA
94%
234
307
TCA
10
35
AA
95%
307
307
TCA
2882
1
AA
94%
307
307
TCA
A
1168
3
AA
83%
283
284
TCA
Fumarase
B
1168
2
AA
74%
191
192
TCA
Fumarase
A
2
56
AA
83%
283
284
TCA
Fumarase
B
2
55
AA
73%
191
192
TCA
Fumarase
A
2074
1&2
AA
83%
275
284
TCA
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Fumarase
B
2074
1
AA
73%
191
192
TCA
Enolase
528
10
AA
99%
425
425
Enolase
3
47
AA
98%
425
425
Enolase
363
4
AA
99%
425
425
Phosphoglycerate Mutase
Phosphoglycerate Mutase
Phosphoglycerate Mutase
Phosphoglycerate Mutase
Phosphoglycerate Mutase
Phosphoglycerate Mutase
Phosphoglycerate Kinase
Phosphoglycerate Kinase
Phosphoglycerate Kinase
Glyceraldehyde-3P Dehydrogenase
Glyceraldehyde-3P Dehydrogenase
Glyceraldehyde-3P Dehydrogenase
Triose-P
Isomerase
Triose-P
Isomerase
907
8
AA
93%
394
394
747
9
AA
92%
514
514
4
109
AA
93%
394
394
4
71
AA
93%
514
514
212
2
AA
93%
394
394
155
15
AA
92%
514
514
642
7
AA
94%
413
413
1
157
AA
94%
413
413
193
3
AA
94%
413
413
263
5
AA
96%
334
334
8
4
AA
96%
334
334
614
3
AA
96%
334
334
1522
3
AA
94%
222
222
6
3
AA
93%
222
222
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Triose-P
Isomerase
Fructose-BP
Aldolase
Fructose-BP
Aldolase
Fructose-BP
Aldolase
Fructose-1,6Bisphosphatase
ADP-specific
phosphofructokina
se
Fructose-1,6Bisphosphatase
ADP-specific
phosphofructokina
se
Fructose-1,6Bisphosphatase
ADP-specific
phosphofructokina
se
Glucose-6-P
Isomerase
Glucose-6-P
Isomerase
Glucose-6-P
Isomerase
D-arabino-Hex-3ulose-6-P
isomerase
D-arabino-Hex-3ulose-6-P
isomerase
D-arabino-Hex-3-
156
2
AA
93%
222
222
1513
5
AA
97%
381
381
4
89
AA
97%
381
381
198
14
AA
97%
381
381
379 &
5151
227
12 & 2
AA
96%
350
362
2
AA
95%
488
491
1
151
AA
96%
362
362
1
163
AA
95%
491
491
94
12
AA
96%
362
362
193
9
AA
95%
491
491
870
2
AA
91%
243
243
1
8
AA
91%
243
243
433
6
AA
91%
243
243
1107
4
AA
94%
200
200
13
32
AA
97%
200
200
181
13
AA
97%
200
200
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Glyconeogen
-esis
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Mh bin
Marcellus Day 328
Mh bin
ulose-6-P
isomerase
3-Hexulose-6-P
Synthase
Methanohalophilus
sp. fracturphilus
68
4
AA
94%
416
416
3-Hexulose-6-P
Synthase
8
63
AA
94%
416
416
Utica Day 98
Mh bin
3-Hexulose-6-P
Synthase
326
4
AA
94%
416
416
Marcellus Day 328
Mh bin
Ribose-5-P
Isomerase
480
6
AA
97%
230
230
Methanohalophilus
sp. fracturphilus
Ribose-5-P
Isomerase
0
140
AA
96%
230
230
Utica Day 98
Mh bin
Ribose-5-P
Isomerase
442
6
AA
97%
230
230
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Aspartate
Transaminase
Aspartate
Transaminase
Aspartate
Transaminase
Aspartate Kinase
1107
5
AA
92%
368
368
13
33
AA
93%
368
368
181
14
AA
92%
368
368
2105
1
AA
95%
460
460
Methanohalophilus
sp. fracturphilus
Aspartate Kinase
4
107
AA
95%
460
460
Utica Day 98
Mh bin
Aspartate Kinase
922
1
AA
95%
460
460
Marcellus Day 328
Aspartate
263
19
AA
93%
333
333
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Partial
Pentose
Phosphate
Oxaloacetate
-> Aspartate
Oxaloacetate
-> Aspartate
Oxaloacetate
-> Aspartate
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
Preparation
Mh bin
Marcellus Day 328
Mh bin
Semialdehyde
Dehydrogenase
Aspartate
Semialdehyde
Dehydrogenase
Aspartate
Semialdehyde
Dehydrogenase
2-amino-3,7dideoxy-D-threohept-6-ulosonate
synthase
2-amino-3,7dideoxy-D-threohept-6-ulosonate
synthase
2-amino-3,7dideoxy-D-threohept-6-ulosonate
synthase
3-dehydroquinate
Synthase
Methanohalophilus
sp. fracturphilus
3-dehydroquinate
Synthase
6
8
AA
97%
380
380
Utica Day 98
Mh bin
3-dehydroquinate
Synthase
156
7
AA
96%
380
380
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Pantoate Kinase
242
1&2
AA
90%
290
291
Pantoate Kinase
7
45
AA
90%
290
291
Pantoate Kinase
222
7
AA
90%
290
291
Pantothenate
242
5
AA
91%
247
247
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Shikimate
Pathway
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
8
18
AA
93%
330
333
2101
2
AA
93%
330
333
2021
5
AA
98%
265
265
6
9
AA
99%
265
265
Preparation
Shikimate
Pathway
156
8
AA
98%
265
265
Preparation
Shikimate
Pathway
2021
4
AA
96%
380
380
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
Preparation
Shikimate
Pathway
CoA
Synthesis
CoA
Synthesis
CoA
Synthesis
CoA
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Synthetase
Pantothenate
Synthetase
Pantothenate
Synthetase
Phosphopantothenoyl-cysteine
sythetase/
decarboxylase
Phosphopantothenoyl-cysteine
sythetase/
decarboxylase
Phosphopantothenoyl-cysteine
synthetase/
decarboxylase
Pantetheinephosphate
adenylyltransferase
Pantetheinephosphate
adenylyltransferase
Pantetheinephosphate
adenylyltransferase
Aspartate
Decarboxlase
Aspartate
Decarboxlase
Aspartate
Decarboxlase
Synthesis
CoA
Synthesis
CoA
Synthesis
CoA
Synthesis
7
48
AA
94%
247
247
222
4
AA
94%
247
247
1433
2
AA
92%
402
402
7
44
AA
93%
402
402
CoA
Synthesis
222
8
AA
93%
402
402
CoA
Synthesis
1324
4
AA
92%
155
154
CoA
Synthesis
6
13
AA
92%
155
154
CoA
Synthesis
156
12
AA
91%
155
154
CoA
Synthesis
1849
3
AA
93%
327
327
3
85
AA
93%
327
327
791
1
AA
94%
327
327
Beta-Alanine
synthesis
Beta-Alanine
synthesis
Beta-Alanine
synthesis
S.T. = Stitched together from multiple scaffolds
** = putative citrate synthase protein sequence from Methanococcoides burtonii
B) Metabolic Features: Energy Metabolism
Genome Analyzed
Protein Encoded
Gene
Name or
Subunit
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Trimethylamine
Corrinoid
Methyltransferase
Trimethylamine
Corrinoid
Methyltransferase
Trimethylamine
Corrinoid
Methyltransferase
Trimethylamine
Corrinoid Protein
Trimethylamine
Corrinoid Protein
Trimethylamine
Corrinoid Protein
Dimethylamine
Corrinoid
Methyltransferase
Dimethylamine
Corrinoid
Methyltransferase
Dimethylamine
Corrinoid
Methyltransferase
Dimethylamine
Corrinoid Protein
Dimethylamine
Corrinoid Protein
Mttb
Scaffold(
s)
Gene(s)
AA or NT
Alignment
%ID to
M. Mahii
Ref Seq
Alignment
Length
932
3&4
AA
91%
495
Mttb
Length
mahii
Ref Seq
(aa)
495
Part of
Metabolism
495
Substrate
Catabolism
Substrate
Catabolism
Mttb
2278
2&3
AA
90%
487
495
Substrate
Catabolism
Mttc
932
5
AA
94%
216
216
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Mttc
216
Mttc
1341
1
AA
94%
215
216
Mtbb
4231 &
26017
S.T.
AA
95%
462
462
Mtbb
7
27 & 28
AA
95%
463
462
Substrate
Catabolism
Mtbb
S.T.
NT
94%
1389
1389
Substrate
Catabolism
Mtbc
2476,
7155,
10262, &
10769
4231
1
AA
98%
203
203
Mtbc
7
26
AA
97%
203
203
Substrate
Catabolism
Substrate
Catabolism
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Dimethylamine
Corrinoid Protein
Monomethylamine
Corrinoid
Methyltransferase
Monomethylamine
Corrinoid
Methyltransferase
Monomethylamine
Corrinoid
Methyltransferase
Mtbc
2476
2
AA
98%
203
203
Substrate
Catabolism
Substrate
Catabolism
Mtmb
5568 &
7141
S.T.
AA
97%
456
457
Mtmb
S.T.
NT
93%
1376
1376
Substrate
Catabolism
S.T.
NT
93%
1376
1377
Substrate
Catabolism
2
AA
98%
216
217
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Mtmc
Mtmc
8 & 35
S.T.
NT
95%
654
654
Mtmc
2866 &
6414
2853 &
3829
S.T.
NT
95%
654
654
S.T.
NT
93%
1384
1386
Mtab
0
296
AA
96%
461
461
Substrate
Catabolism
Mtab
926
1
AA
96%
461
461
Substrate
Catabolism
Marcellus Day 328
Mh bin
Monomethylamine
Corrinoid Protein
Monomethylamine
Corrinoid Protein
Monomethylamine
Corrinoid Protein
Methanol
Corrinoid
Methyltransferase
Methanol
Corrinoid
Methyltransferase
Methanol
Corrinoid
Methyltransferase
Methanol
Corrinoid Protein
10, 36,
49, 72,
75, & 76
1054,
2866,
7813, &
10693
7141
Mtac
7778
AA
93%
254
254
Substrate
Catabolism
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanol
Corrinoid Protein
Methanol
Corrinoid Protein
Methylamine CoM
methyltransferase
Mtac
0
Orf is
majority of
scaffold
295
AA
93%
254
254
Mtac
926
2
AA
95%
254
254
Mtba
1332 &
8312
S.T.
NT
96%
1029
1029
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Mtmb
Mtab
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methylamine CoM
methyltransferase
Methylamine CoM
methyltransferase
Methanol CoM
methyltransferase
Methanol CoM
methyltransferase
Methanol CoM
methyltransferase
Reductive
Activation of
methyltransfer
Amines
Reductive
Activation of
methyltransfer
Amines
Reductive
Activation of
methyltransfer
Amines
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Mtba
6
45
AA
97%
342
342
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Substrate
Catabolism
Mtba
1973
2
AA
97%
342
342
Mtaa
248
11
AA
94%
338
338
Mtaa
0
89
AA
93%
338
338
Mtaa
444
9
AA
93%
338
338
Rama
932
2
AA
66%
538
540*
Rama
1
139
AA
66%
538
540*
Substrate
Catabolism
Rama
1406
1
AA
67%
538
540*
Substrate
Catabolism
Mtra
914
6
AA
93%
223
240
Methanogenesis
Mtrb
914
5
AA
96%
107
107
Methanogenesis
Mtrc
914
4
AA
91%
258
271
Methanogenesis
Mtrd
914
3
AA
99%
216
239
Methanogenesis
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
Mtre
914
2
AA
96%
302
302
Methanogenesis
Mtrf
914
7
AA
99%
75
75
Methanogenesis
Mtrg
914
8
AA
99%
77
77
Methanogenesis
Mtrh
914
9
AA
100%
318
318
Methanogenesis
Mtra
12
21
AA
93%
223
240
Methanogenesis
Mtrb
12
20
AA
96%
107
107
Methanogenesis
Mtrc
12
19
AA
91%
258
271
Methanogenesis
Mtrd
12
18
AA
98%
239
239
Methanogenesis
Mtre
12
17
AA
97%
302
302
Methanogenesis
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
opterin : CoM
Methyltransferase
Methyl-
Mtrf
12
22
AA
99%
75
75
Methanogenesis
Mtrg
12
23
AA
99%
77
77
Methanogenesis
Mtrh
12
24
AA
99%
318
318
Methanogenesis
Mtra
67
19
AA
94%
223
240
Methanogenesis
Mtrb
67
20
AA
95%
107
107
Methanogenesis
Mtrc
67
21
AA
91%
258
271
Methanogenesis
Mtrd
67
22
AA
98%
239
239
Methanogenesis
Mtre
67
23
AA
97%
302
302
Methanogenesis
Mtrf
67
18
AA
99%
75
75
Methanogenesis
Mtrg
67
17
AA
99%
77
77
Methanogen-
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Tetrahydromethan
opterin : CoM
Methyltransferase
MethylTetrahydromethan
-opterin : CoM
Methyltransferase
Methylenetetrahydromethanopterin reductase
Methylenetetrahydromethanopterin reductase
Methylenetetrahydromethanopterin reductase
Methylenetetrahydromethanopterin
dehydrogenase
Methylenetetrahydromethanopterin
dehydrogenase
Methylenetetrahydromethanopterin
dehydrogenase
Methylenetetrahydromethanopterin
cyclohydrolase
Methylenetetrahydromethanopterin
cyclohydrolase
Methylene-
esis
Mtrh
67
16
AA
99%
318
318
Methanogenesis
448
10
AA
99%
328
328
Methanogenesis
2
244
AA
99%
328
328
Methanogenesis
520
1
AA
99%
328
328
Methanogenesis
71
8
AA
97%
278
278
Methanogenesis
0
8
AA
97%
278
278
Methanogenesis
392
3
AA
97%
278
278
Methanogenesis
880
13
AA
97%
319
319
Methanogenesis
20
2
AA
97%
319
319
Methanogenesis
448
8
AA
97%
319
319
Methanogen-
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
tetrahydromethanopterin
cyclohydrolase
Formylmethanofuran :
tetrahydromethanopterin
formyltransferase
Formylmethanofuran :
tetrahydromethanopterin
formyltransferase
Formylmethanofuran :
tetrahydromethanopterin
formyltransferase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formyl-
esis
7126 &
28507
S.T.
NT
95%
894
894
Methanogenesis
3
76
AA
98%
297
297
Methanogenesis
967
3
AA
98%
297
297
Methanogenesis
362,
7589, &
15325
5581
S.T.
NT
95%
1700
1758
Methanogenesis
2
AA
97%
423
435
Methanogenesis
C
7378 &
13673
S.T.
NT
91%
812
810
Methanogenesis
D
5581
3
AA
95%
129
129
Methanogenesis
E
362
3
AA
95%
201
201
Methanogenesis
F
362
2
AA
96%
341
341
Methanogenesis
G
1099
7
AA
93%
153
153
Methanogen-
A
B
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
methanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formyl-
esis
A
2
44
AA
98%
585
585
Methanogenesis
B
2
47
AA
97%
435
435
Methanogenesis
C
2
45
AA
93%
263
269
Methanogenesis
D
2
46
AA
98%
129
129
Methanogenesis
E
2
42
AA
94%
199
201
Methanogenesis
F
2
43
AA
95%
341
341
Methanogenesis
G
0
273
AA
92%
153
153
Methanogenesis
A
147
4
AA
98%
585
585
Methanogenesis
B
147
7
AA
97%
435
435
Methanogenesis
C
147
5
AA
93%
269
269
Methanogenesis
D
147
6
AA
98%
129
129
Methanogenesis
E
147
2
AA
94%
199
201
Methanogen-
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
methanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Formylmethanofuran
dehydrogenase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
Heterodisulfide
Reductase
esis
F
147
3
AA
96%
341
341
Methanogenesis
G
183
6
AA
91%
153
153
Methanogenesis
A
2215
2
AA
96%
784
784
B
284
AA
92%
311
311
C
284
9
AA
94%
201
201
D
1987
8
AA
97%
418
418
E
1987
9
AA
98%
258
258
A
0
276
AA
95%
784
784
B
12
27
AA
93%
295
311
C
12
28
AA
92%
161
201
D
5
46
AA
97%
418
418
E
5
47
AA
97%
258
258
A
183
3
AA
95%
784
784
B
67
13
AA
93%
295
311
C
768
2
AA
94%
201
201
D
1616
2
AA
98%
418
418
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Methanogenesis
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Heterodisulfide
Reductase
RNF
E
1616
1
AA
98%
254
258
A
574
18
AA
91%
143
197
RNF
B
4317
AA
93%
265
265
RNF
C
574
14
AA
91%
451
451
RNF
E
574
17
AA
87%
205
205
RNF
G
574
16
AA
66%
189
183
RNF
A
1
131
AA
88%
196
197
RNF
B
1
132
AA
93%
265
265
RNF
C
1
127
AA
92%
451
451
RNF
E
1
130
AA
87%
205
205
RNF
G
1
129
AA
66%
189
183
RNF
A
296
3
AA
88%
196
197
RNF
B
296
2
AA
94%
265
265
RNF
C
296
7
AA
92%
451
451
RNF
E
296
4
AA
87%
205
205
RNF
G
296
5
AA
66%
189
183
F420
Dehydrogenase
F420
Dehydrogenase
A
435
2
AA
86%
132
132
B
5946
2
AA
96%
191
191
Methanogenesis
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
C
5946
1
AA
94%
130
163
C
435
4
AA
75%
163
163
D
435
5
AA
86%
374
374
F
448
9
AA
96%
342
342
H
2992
2
AA
99%
342
342
I
2992
3
AA
97%
137
137
K
435
10
AA
93%
100
100
L
435
11
AA
98%
670
670
M
435
12
AA
97%
479
495
N
435
13
AA
97%
483
483
O
435
14
AA
96%
120
120
A
11
89
AA
86%
132
132
B
11
88
AA
81%
185
191
C
11
87
AA
75%
163
163
D
11
86
AA
86%
374
374
F
2
243
AA
96%
342
342
H
11
85
AA
85%
341
342
I
11
84
AA
90%
137
137
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
F420
Dehydrogenase
ATPase
K
11
81
AA
93%
100
100
L
11
80
AA
98%
670
670
M
11
79
AA
98%
479
495
N
11
78
AA
97%
483
483
O
11
77
AA
96%
120
120
A
946
5
AA
86%
132
132
B
946
4
AA
81%
185
191
C
946
3
AA
75%
163
163
D
946
2
AA
86%
374
374
F
520
2
AA
96%
342
342
H
S.T.
AA
85%
341
342
I
320 &
946
320
2
AA
90%
137
137
K
320
5
AA
93%
100
100
L
320
6
AA
98%
670
670
M
320
7
AA
98%
479
495
N
320
8
AA
97%
483
483
O
320
9
AA
96%
120
120
A
507
13
AA
98%
576
576
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
ATPase
B
507
12
AA
98%
460
460
ATPase
C
S.T.
NT
88%
1077
1074
ATPase
D
507 &
124
507
11
AA
98%
205
205
ATPase
E
124
27
AA
95%
183
183
ATPase
F
507
14
AA
90%
100
100
ATPase
H
124
24
AA
98%
108
108
ATPase
I
124
25
AA
79%
670
670
ATPase
A
2
181
AA
98%
576
576
ATPase
B
2
180
AA
98%
460
460
ATPase
C
2
183
AA
92%
357
357
ATPase
D
2
179
AA
98%
205
205
ATPase
E
2
184
AA
95%
183
183
ATPase
F
2
182
AA
90%
100
100
ATPase
H
2
187
AA
98%
108
108
ATPase
I
2
186
AA
79%
670
670
ATPase
A
235
10
AA
98%
576
576
ATPase
B
235
9
AA
98%
460
460
ATPase
C
235 &
959
12 & 4
AA
93%
359
357
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
ATPase
D
235
8
AA
98%
205
205
ATPase
E
959
3
AA
93%
183
183
ATPase
F
235
11
AA
91%
100
100
ATPase
H
5201
AA
98%
108
108
ATPase
I
959
1
AA
78%
642
670
Methylornithine
Synthase
3-methylornithineL-Lysine Ligase
3-methylornithylN6L-Lysine
Dehydrogenase
Pyrrolysine t-RNA
Synthetase
Methylornithine
Synthase
3-methylornithineL-Lysine Ligase
3-methylornithylN6L-Lysine
Dehydrogenase
Pyrrolysine t-RNA
Synthetase
Methylornithine
Synthase
3-methylornithineL-Lysine Ligase
3-methylornithylN6-
pylB
1332 &
1457
1332 &
1457
1332 &
1457
2&4
AA
346 & 346
346
3&3
AA
360 & 360
360
4&2
AA
95% &
96%
93% &
90%
95% &
95%
259 & 259
259
1&5
AA
404
48
AA
94% &
94%
96%
405 & 404
pylB
1332 &
1457
6
346
346
pylC
6
47
AA
90%
360
360
pylD
6
46
AA
97%
259
259
pylS
6
49
AA
93%
404
404
pylB
1463
2
AA
96%
346
346
pylC
1463 &
6383
1973 &
6383
S.T.
NT
93%
1085
1083
S.T.
NT
93%
780
780
pylC
pylD
pylS
pylD
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Energy
Conservation
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Pyrrolysine
Biosynthesis
Utica Day 98
Mh bin
L-Lysine
Dehydrogenase
Pyrrolysine t-RNA
pylS
656 &
S.T.
NT
92%
1215
Synthetase
1463
* This reference protein sequence was pulled from Methanosarcina barkeri
C) Metabolic Features: Osmo Protection
Genome Analyzed
Protein Encoded
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Methanohalophilus
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Marcellus Day 328
Mh bin
Methanohalophilus
sp. fracturphilus
Methanohalophilus
Gene
Name or
Subunit
TrkH
TrkA
TrkA
N
terminus
C
terminus
TrkH
TrkA
TrkA
N
terminus
C
terminus
TrkH
TrkA
TrkA
Glycine Sarcosine
Methyltransferase
Sarcosine DMG
Methyltransferase
Glycine Sarcosine
Methyltransferase
Sarcosine DMG
N
terminus
C
terminus
1215
Pyrrolysine
Biosynthesis
Part of
Metabolism
Scaffold(
s)
Gene(s)
AA or NT
Alignment
%ID to
M. Mahii
Ref Seq
Alignment
Length
3598 &
7370
1868
S.T.
NT
92%
1350
Length
mahii
Ref Seq
(aa)
1350
2
AA
93%
444
445
71
41
AA
99%
400
400
3
71
AA
91%
450
479
1
197
AA
93%
444
445
0
62
AA
98%
400
400
267
1
AA
91%
450
479
342
5
AA
93%
444
445
1899
1
AA
98%
336
400
242
10
AA
95%
263
263
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
Potassium
Transport
GB synthesis
242
11
AA
92%
278
278
GB synthesis
7
53
AA
95%
263
263
GB synthesis
7
54
AA
92%
278
278
GB synthesis
sp. fracturphilus
Utica Day 98
Mh bin
Utica Day 98
Mh bin
Marcellus Day 328
Mh bin
Methyltransferase
Glycine Sarcosine
Methyltransferase
Sarcosine DMG
Methyltransferase
Lysine-2,3Aminomutase
Marcellus Day 328
Mh bin
76
2
AA
95%
263
263
GB synthesis
76
3
AA
92%
278
278
GB synthesis
1136
4
AA
98%
358
358
Beta-Lysine
Acetyltransferase
507
8
AA
88%
276
276
Methanohalophilus
sp. fracturphilus
Lysine-2,3Aminomutase
7
11
AA
98%
358
358
Methanohalophilus
sp. fracturphilus
Beta-Lysine
Acetyltransferase
7
176
AA
88%
276
276
Utica Day 98
Mh bin
Lysine-2,3Aminomutase
2159
1
AA
98%
358
358
Utica Day 98
Mh bin
Beta-Lysine
Acetyltransferase
235
5
AA
88%
276
276
N-Acetyl-BLysine
synthesis
N-Acetyl-BLysine
synthesis
N-Acetyl-BLysine
synthesis
N-Acetyl-BLysine
synthesis
N-Acetyl-BLysine
synthesis
N-Acetyl-BLysine
synthesis