From: Synthesis and Secretion of Hemoglobin by Retinal Pigment Epithelium
Invest. Ophthalmol. Vis. Sci.. 2009;50(4):1911-1919. doi:10.1167/iovs.07-1372
Figure Legend:
Immunoelectron microscopy of human RPE and Bruch’s membrane. (A) Representative ultrathin sections (50 nm) of
human RPE (n = 2, 81 and 64 years old) revealed the presence of hemoglobin in human RPE cytosol within multiple
round, intermediate-density cytoplasmic granules (arrows). (B, C) At higher magnifications hemoglobin labeling was
confined to the RPE cytoplasm, mainly as intermediate-density granules. (D) Human RPE cells excreted hemoglobin
from the basal side toward Bruch’s membrane (arrow). Excreted hemoglobin accumulated mainly within the inner
collagen layer (ICL) of Bruch’s membrane as electron-dense material (arrowhead). (E) At a higher magnification,
hemoglobin
immunostaining
was observed
within porosome-like
vesicles
(arrows)
lining theCopyright
basal infoldings
RPE
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basal membrane (BM). Some vesicles fused with the BM and released hemoglobin toward the ICL (white arrows). (F)