THINGS TO RESEARCH: temperature of wildfires o importance o data tables o the effects (short and long) o how varied it can be (min and max fire temp) o effects on bacteria bacteria of N -cycle o importance o types of bacteria and their role N-cycle o importance in the real world Soil o layers of soil' o why it's important o functions o background info SITES WITH STATISTICS http://www.agf.gov.bc.ca/range/publications/documents/fire2.htm http://databasin.org/maps/new#datasets=41d3c8b8694342b482357480e34ad42b http://www.fs.fed.us/rm/pubs/rmrs_gtr042_4.pdf http://www.talltimbers.org/images/fireecology/Wildfire-PF-ClimateChange6-07.pdf http://news.nationalgeographic.com/news/2013/08/130827-wildfires-yosemite-fire-firefightersvegetation-hotshots-california-drought/ http://www.climatechoices.org/impacts_wildfires/ http://www.energy.ca.gov/2005publications/CEC-500-2005-192/CEC-500-2005-192-SF.PDF http://headwaterseconomics.org/wildfire/temperatures-cost-safety http://www.ucsusa.org/global_warming/science_and_impacts/impacts/global-warming-andwildfire.html http://eco.ib.usp.br/lepac/conservacao/Artigos/Pivello_et_al_2010.pdf http://landresources.montana.edu/Department/PDF/2008_Capstone.pdf http://abe.ufl.edu/carpena/files/pdf/research/reports/chung%20nguyen_dissertation.pdf http://www.nps.gov/yell/naturescience/wildlandfire.htm Research: nitorsomonas- its major source of energy and reducing power is from the oxidation of ammonia to nitrate There’s gas and water in soil that occupy the gaps between minerals. They constantly shift states between liquid and gas to maintain equilibrium Soil particle sizes are split up into three sizes (from largest to smallest): sand, silt, and clay Made of plant nutrients, potassium, calcium, and magnesium www.nerrs.noaa.gov/doc/siteprofile/acebasin/html/envicond/soil/slform.htm Yoooooo, here are some tables we can use. Feel free to add or remove from this list. Neary, D. G., Ryan, K. C., & DeBano, L. F. (2005, September). Wildland fire in ecoysystems: Effects of fire on soil and water [PDF]. Retrieved from http://www.fs.fed.us/rm/pubs/rmrs_gtr042_4.pdf Humans are about 3% nitrogen which is used to make amino acids and proteins Nitrogen Cycle 1. Nitrogen Fixation a. Atmospheric N2 is changed to ammonia (NH3) by nitrogen fixing bacteria in roots 2. Nitrification a. ammonia (NH3) to ammonium (NH4) by adding water to Nitrite (NO2) to Nitrate (NO3) b. performed by bacteria 3. Assimilation a. nitrogen taken into roots and become part of the food chain i. In plants, animals eat plants ii. NO3 (nitrate) changed back into organic nitrogen 4. Ammonification a. when plants and animals decomposed Organic nitrogen in them is changed back into ammonia and ammonium to be recycled 5. Denitrification a. denitrifying bacteria return Nitrates to Atmospheric nitrogen (N2) slideshare.net Picture above is of Nitrification (www.fbas.co.uk/nitrogen%20cycle.jpeg) Lethal Temps for Nitrosomonas (shown above) involved in nitrification 140 degrees C in dry soil 75 C in wet soil Lethal temps for microorganisms in general greater than 50 C - microorganisms begin to die (couldn't find specific temp for Nitrobacter) Nitrosomonas and Nitrobacter are used to convert Ammonia (from N2) in to a form that is more usable for plants Temperature (http://www.bioconlabs.com/nitribactfacts.html) The temperature for optimum growth of nitrifying bacteria is between 7786° F (25-30°C). Growth rate is decreased by 50% at 64° F (18° C). Growth rate is decreased by 75% at 46-50° F. No activity will occur at 39° F (4° C) Nitrifying bacteria will die at 32° F (0° C). Nitrifying bacteria will die at 120° F (49° C) Nitrobacter is less tolerant of low temperatures than Nitrosomonas. In cold water systems, care must be taken to monitor the accumulation of nitrites. Measuring Mircoorganisms http://ohioline.osu.edu/sag-fact/pdf/0016.pdf - there is a table of average biomass that it wouldn't let me copy Soil respiration reflects amount of microorganisms in soil use metabolic quotient also called specific respiratory rate which is defined as the microbial respiration rate per unit microbial biomass o determined by substrate induced respiration (http://www.neri.dk/1_viden/2_Publikationer/3_fagrapporter/rapporter /FR388.pdf) Microbial Biomass (http://soilquality.org.au/factsheets/category/biologicalindicators) o Microbial biomass is an important indicator of soil health because it is closely related to nutrient release from crop residues. The microbial biomass consists mostly of bacteria and fungi, which decompose crop residues and organic matter in soil. This process releases nutrients, such as nitrogen (N), into the soil for plant uptake. About half the microbial biomass is located in the surface 10 cm of soil and most of the nutrient release also occurs here. Microbial Biomass - Queensland o The soil microbial biomass consists mostly of bacteria and fungi, which decompose crop residues and organic matter in soil. The microbial biomass typically makes up less than 5 % of total soil organic matter, but it plays a very large role in a number of key soil functions, including nutrient release, the maintenance of good soil structure and the suppression of plant pathogens. Changes to the microbial biomass can also be an early indicator of changes in total soil organic carbon. Unlike total organic carbon, microbial biomass carbon responds quickly to management changes, and can often be measured before changes in total organic carbon are detected. file:///C:/Users/lightning/Downloads/Blagodatsky_Heinemeyer_Richter_00_ BFS%20(1).pdf- pg 2-5 gives method on how to measure Let me know if u have any questions- Brytani Method In order to conduct this investigation, Samples will be collected from places around the US that have not experienced a fire in at least 10 years (feel free to revise) to eliminate the affects of that fire. For example, samples from Yellowstone National Park will be taken as it has not experienced a fire since 1988, leaving time for the effects of the fire to no long be at play. To measure microorganisms, two samples of soil will be taken. One untreated and one fumigated to kill off organisms. Sugar or an energy sources is added to the untreated sample to increase organism activity. Comparing the Carbon Dioxide released can be used to determine the mass of the organisms in the soil. Carbon Dioxide is measured through a laboratory respirometer which measures carbon dioxide release of samples. (http://www.bioscienceinc.com/product/bi-2000laboratory-respirometer/) for examples the BI2000 Respirometer by bioscience incorporated. (pictures are on the link before this sentence). After finding the carbon dioxide levels released, using the metabolic quotient, the estimate of microorganisms biomass can be found. The majority of the microorganisms are fungi and bacteria. Although this does not specifically give the amount of bacteria that are involved in the Nitrogen cycle, it does provide an estimate.
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