Curriculum Vitae
Dr. VIJAY KUMAR
M.Sc., PhD (NET)
Contact: +91-7520613320
E-mail: [email protected]
Research Interest:
Applied and Industrial Microbiology, Molecular microbiology, Medical microbiology, Food
Microbiology, Fermentation Technology, Natural products from Plant and Microorganisms
Objective:
To achieve a challenging position in an accredited institute/industry that will harness my knowledge,
experience, expertise and enable me to effectively contribute to the organization/industry and expand
my mental horizon.
Academics:
 Qualified ICAR NET-2013 (Agricultural Microbiology)

PhD: Biotechnology (August 2008-May 2013) Antibacterial metabolites from actinomycetes of
diverse habitats and locations of Uttarakhand” in the Biotechnology Department of H.N.B. Garhwal
Central University, Srinagar, Garhwal . Worked as JRF from (June 2007- May 2009) and SRF from
(June-2009-May 2011).

Post graduation: (2004-2006) Master of Science in Microbiology from H.N.B. Garhwal University,
Garhwal (A Central University).

Graduation: (1999-2002) Bachelor of Science with (Chemistry, Botany and Zoology as majors)
from St. Andrew’s PG College, Gorakhpur, UP
Abstract of PhD thesis:
Searching new antibiotics has increased worldwide because of the serious problem of antibiotic
resistance among the microbes. The recent discovery of novel primary and secondary metabolites
from taxonomically unique population of actinomycetes suggest that these organisms could add a
new dimension to microbial natural product research. The history of new drug discovery processes
shows that novel skeletons have, in the majority of cases, come from natural sources. Today, the
emphasis is on the exploration of unusual and previously ignored ecosystems. Actinomycetes from
several unexplored environments have been intensively studied in last few decades for novel and
potent molecules. In this scenario, this study was focused on solitary wasp mud nest, swallow bird
mud nest, earthworm castings, hot water springs, and plant endophytes as well as soil samples
collected from different locations of Uttarakhand other than unusual habitats. On the basis of the
evidence obtained from the present study some general conclusions can be drawn that the solitary
wasp mud nest, swallow bird mud nest, and earth worm castings are very productive ecosystems with
regard to the occurrence of microbial flora. The results of the present study strongly support the idea
that actinomycetes species isolated from solitary wasp mud nest, swallow bird mud nest and
earthworm castings possess a significant capacity to produce compounds having unique antibacterial
activity as compared to terrestrial isolates. The isolates 8(1)*, MN 9(V), MN 2(6) and EWC 7(2) were
found to be most prominent in the terms of desirable activities. These isolates were found to have
excellent antimicrobial potential against several drug resistant bacterial pathogens. Preliminary
characterization of the metabolites produced by Streptomyces sp. 8(1)* and Streptomyces sp. EWC
7(2) indicates that it may be novel metabolites however further studies are required to elucidate its
structure to confirm novelty. The activity of gentamicin and cefotaxime were enhanced by the
presence of the metabolite produced by Streptomyces sp 7(2) against Gram-positive and Gram
negative bacteria including drug resistant strains. Similarly, the activity of piperacillin, gentamicin and
penicillin showed significantly improved activity against both Gram-positive and Gram-negative
bacterial strains when combined with the metabolite of Streptomyces sp. 8 (1)*. However, further
elaborate chemical, biochemical and pharmacological studies are necessary to develop these active
principles to the level of a drug.
Research Experience
S.B.S.P.G.I. Dehradun, Uttarakhand
June 2009- Feb 2011 (2 Years)
As SRF: Worked as a Senior Research Fellow in the project entitled “Searching potential metabolites
from Actinomycete active against drug resistant bacterial pathogens” funded by Uttarakhand council
for Science and Technology.
S.B.S.P.G.I. Dehradun, Uttarakhand
June 2007- May 2009
(2 Years)
As JRF: Junior Research Fellow in the project entitled “Searching potential metabolite from
Actinomycetes active against drug resistant bacterial pathogens” funded by Uttarakhand council for
Science and Technology.
S.B.S.P.G.I. Dehradun, Uttarakhand
31 January 2006 – 15 June 2006
M.Sc. Dissertation work had been done on the topic: “Isolation, characterization and phylogenetic
analysis of α-class of proteobacteria isolated from root nodules of Mimosa pudica”
Teaching Experience
Doon PG College of Agriculture Science and Technology
19 June 2013 to Till date
As a Assistant professor in Department of Food Technology
27th Feb. 2012 to 8th june 2013
Baba Farid Institute of Technology

As a Assistant professor in Department of Microbiology
April 2009 – December 2009
S.B.S.P.G.I. Dehradun, Uttarakhand

As a teaching assistant in Department of Microbiology, S.B.S. P.G.I. Balawala, Dehradun.
Computer Knowledge:
Well versed with MS-Office (Word, Excel and Power Point), Internet explorer and Mozilla firefox
Operating systems (O/S): Windows 2000/ XP/ Vista
Softwares: SwissPdbViewer, eztaxon, Primer BLAST, BLAST, MINITAB, STATISTICA, MEGA 4, 5 &
6 versions, Phylip, Phyedit
Applications: SigmaPlot-10, Origin, ClustalW
List of Publications
Published/Accepted (16)
1. Vijay Kumar, Alpana Bharti,Vivek kumar Gupta, Omprakash Gusain and Gajraj Singh Bisht
(2012) Actinomycetes from shallow bird nest, mud wasp nest: Isolation and screening of
actinomycetes
for
their
antimicrobial
activity.
World
Journal
of
Microbiology
and
Biotechnology, 28:871-880. (Springer) (Cited 8 times) PMID: 22805807 [PubMed - indexed for
MEDLINE]
2. Vijay Kumar, Alpana Bharti, Omprakash Gusain, Yogesh Kumar Negi and Gajraj Singh Bisht
(2012) Screening of actinomycetes from earthworm castings for their antimicrobial activity and
industrial enzymes. Brazilian Journal of Microbiology, 42: 205-214. (Cited 5 times) PMID:
24031819 [PubMed] PMCID:PMC3768992
3. Vijay Kumar, Alpana Bharti, Omprakash Gusain, Yogesh Kumar Negi and Gajraj Singh Bisht
(2011) Taxonomy and antibacterial activity of moderate salt tolerant, alkaliphillic Streptomyces
sp. MN 9(V) isolated from solitary wasp mud nest. Annals of Microbiology, 62:979-985.
(Springer) (Cited 3 times)
4. Vijay Kumar, Alpana Bharti, Omprakash Gusain and Gajraj Singh Bisht. Scanning electron
microscopy of Streptomyces without using any chemical fixatives (2011) Scanning. 33: 1–4.
DOI: 10.1002.sca.20261. (Willey sciences) (Cited 5 times) PMID: 21732388 [PubMed indexed for MEDLINE] (Cover Image)
5. Sudhir Kumar, Nancy, Devendra Kumar, Vijay Kumar (2012) Evaluating the antibacterial activity
of plant extracts against bacterial pathogens. Journal of Drug Delivery & Therapeutics 2(4):
182-185 (Cited 1 time)
6. Vijay Kumar, Alpana Bharti, Omprakash Gusain and Gajraj Singh Bisht (2010). An improved
method for isolation of genomic DNA from filamentous actinomycetes, Journal of Sci. Engg. &
Tech. Mgt., 2: 10-13. (Cited 9 times)
7. Vijay Kumar, Omprakash Gusain, Ramlal Thakur and Gajraj Singh Bisht (2012). Isolation,
purification and partial characterization of antibacterial agent from halotolerant akaliphilic
Streptomyces sp. EWC7 (2). Proceedings of National Academy of Sciences, India Series B
Biological Sciences. DOI 10.1007/s40011-012-0117-y (Springer) (Cited 2 times)
8. Gajraj Singh Bisht , Alpana Bharti, Vijay Kumar and Omprakash Gusain(2012) Isolation, partial
purification and characterization of broad spectrum antifungal agent from salt-tolerant alkalophilic
Streptomyces sp. IN2-10. Proceedings of National Academy of Sciences, India Series B
Biological Sciences 83 (1): 109-117. DOI:10.1007/s40011-012-0086-1 (Springer). (Cited 4
times)
9. Alpana Bharti, Vijay Kumar, Omprakash Gusain and Gajraj Singh Bisht (2011). Isolation,
characterization and antifungal activity of Streptomyces sp. IN1-9 isolated from Garhwal
Himalayas. Journal of Sci. Engg. & Tech. Mgt., 3: 3-8.
10. Alpana Bharti, Vijay Kumar, Omprakash Gusain and Gajraj Singh Bisht (2010). Antifungal
activity of actinomycetes isolated from Garhwal region, Journal of Sci. Engg. & Tech. Mgt., 2:
3-9. (Cited 8 times)
11. Puspendra Sharma, Vijay Kumar, Bindu Naik, Gajraj Singh Bisht (2013) Isolation, screening and
production of Xylanase from terrestrial actinomycetes isolated from various habitat of
Uttarakhand. EurAsian Journal of Biosciences, 7, 30-40.
12. Urvashi Mundepi, Vijay Kumar, Shyamli Gupta, GRS Bisht, FC Garg, Vivek Kumar Gupta,
(2013) Evaluation of anticandidal potential of some folklore plants. Proceedings of National
Academy of Sciences, India Series B Biological Sciences. DOI: 10.1007/s40011-013-0232-4
(Springer)
13. Mragendata Chaturvedi, Vijay Kumar, Devendra Singh and Sudhir Kumar (2013) Assessment of
microbial load of some common vegetables among two different socioeconomic groups.
International Food Research Journal, 20(5): 2927-2931 (published by Faculty of Food Science
and Technology Universiti Putra, Malaysia)
14. Vijay Kumar, Omprakash Gusain and Gajraj Singh Bisht (2013) Terrestrial actinomycetes from
diverse locations of Uttarakhnad, India: Isolation and screening for antibacterial activity, Iranian
Journal of Microbiology, 5(3):299-308. PMID: 24475340 [PubMed]
15. Vishal Kumar, Bindu Naik, Vijay Kumar, Devendra Kumar, Mohammad Aslam, Sudhir Kumar
(2014) Evaluation of antibacterial and anti-oxidant activity of some lichens of Uttarakhand
(Accepted in American Journal of Current Biology)
16. Bindu Naik, Vijay Kumar, Rama Chandra Pradhan (2014): Bioprospecting of enophytic
Actinobacterial Species for their pigments having antioxidant property (Accepted for
publication in Proceedings of National Seminar on Indian Agriculture and Rural
Development in Changing Global Scenario, BHU)
Communicated (05)
1. Vijay Kumar, Omprakash Gusain, Gajraj Singh Bisht, Vivek Kumar Gupta and D. Saikia
(2013). Detection of antimycobacterial activity of some actinomycetes by radiometric
BACTEC assay. (Communicated in Indian Journal of Natural products and Resources,
NISCAIR)
2. Avijit Kumar Awasthi, P. Kumar, Sarika Awasthi, Vijay Kumar, Gajraj Singh Bisht (2013) In
Vitro Antimicrobial Activity of Vanda cristata Lindl: A Rare Orchid. (Communicated in Indian
Journal of Natural products and Resources, NISCAIR )
3. Vijay Kumar, Bindu Naik, Omprakash Gusain, Gajraj Singh Bisht (2013) Taxonomy and
antimicrobial activity of a new actinomycete isolate from solitary wasp mud nest.
(Communicated in 3Biotech, Springer)
4. Helly Singh, Vijay Kumar, Bindu Naik, Gajraj Singh Bisht (2013) Screening of endophytic
actinomycetes for their herbicidal activity. (Communicated in Agricultural Research,
Springer)
5. Vijay Kumar, Bindu Naik, Omprakash Gusain, Gajraj Singh Bisht (2013) Taxonomy and
antimicrobial activity of an actinomycetes strain isolated from Swallow bird mud nest.
(communicated in National Academy Science Letters , Springer)
List of conference papers presented (10)
1. Vijay Kumar, Alpana Bharti, Yogesh Kumar Negi, Omprakash Gusain, Gajraj Singh Bisht,
Screening of actinomycetes from
earthworm castings for their
antimicrobial activity and
industrial enzymes in 5thUttarakhand Science and Technology Congress Held at Doon
University, 10th Dec. 2010
2. Alpana Bharti, Vijay Kumar, Omprakash Gusain, Gajraj Singh Bisht, Antifungal activity of
actinomycetes isolated from garhwal region in 5thUttarakhand Science and Technology
Congress Held at Doon University, 10th Dec. 2010
3. Vijay Kumar, Alpana Bharti, Omprakash Gusain, Gajraj Singh Bisht, Simple, fast and reliable
method for extraction of genomic DNA from filamentous actinomycetes” in 50 th Annual
conference of AMI (Association of Microbiologists of India), December, 2009.
4. Gajraj Singh Bisht, Vijay Kumar, Alpana Bharti, Exploring the diversity of antibiotic producing
soil actinomycetes in Uttarakhand 4th Uttarakhand Science and Technology Congress, Nov.
2009
5. Alpana Bharti, Vijay Kumar, Omprakash Gusain, Antifungal potential of Actinomycetes
isolated from Garhwal region in National Symposium S.B.S.P.G.I., Balawala, Dehradun, Feb.
2009
6. Alpana bharti, Vijay Kumar, Yogesh Kumar Negi, Evaluation of antifungal activity of
Pseudomonas fluorescence against Magnaporthe grisea causing the blast disease of finger
millet (Eleusine coracana)” in National Symposium on “Advances in Microbial Diversity and
Disease Management for Sustainable” Ranichauri Oct. 2008.
7. Alpana Bharti, Vijay Kumar, Baljit Singh Saharan, Bacteriocin Production and Optimization
using isolates from curd and milk” in 3rd Uttarakhand Science and Technology Congress, IIT
Roorkee, Nov. 2008
8. Vijay Kumar, Alpana Bharti, Yogesh Kumar Negi, Omprakash Gusain, Gajraj Singh Bisht,
Antibacterial potential of Actinomycetes isolated from Garhwal region in 3 rd Uttarakhand
Science and Technology Congress, IIT, Roorkee, Nov. 2008
9. Vijay Kumar, Piyush Pandey, Anchal Sood, Peter Young, Cyril Bontemps, Different Rhizobia
from root nodules of Mimosa pudica, in National conference on Microbial Diversity: Avenues
and Applications at S.B.S.P.G.I, Balawala, Dehradun, Feb 2007.
10. Bindu Naik, Vijay Kumar, Rama Chandra Pradhan(2014): Bioprospecting of enophytic
Actinobacterial Species for their pigments having antioxidant property, National Seminar on
Indian Agriculture and Rural Development in Changing Global Scenario, BHU.
Cited in some important publications

M Mehravar, S Sardari, P Owlia (2011) Membrane-active metabolites produced by soil
actinomycetes using chromatic phospholipid/polydiacetylene vesicles. Indian Journal of
Experiment Biology, 49:946-952

Antonella
Amore, Olimpia
Pepe, Valeria
Ventorino, Leila
Birolo, Chiara
Giangrande and Vincenza Faraco.(2012) Cloning and recombinant expression of a cellulase
from the cellulolytic strain Streptomyces sp. G12 isolated from compost. Microbial Cell
Factories, 11:164

Bamzadeh Zahra; Baserisalehi, Majid; Bahador, Nima; Hejazi, Seyed Hossein (2013).
Screening of soil Streptomyces and characterization of their bioactive compounds.
HealthMed 8:2293-2300

Mohseni, Mojtaba; Norouzi, Hamed (2013) Antifungal Activity of Actinomycetes Isolated from
Sediments of the Caspian Sea. Journal of Mazandaran University of Medical Sciences 23:
104:79-87

Ramesh Subramani, William Aalbersberg (2013) Culturable rare Actinomycetes: diversity,
isolation
and
marine
natural
product
discovery.
Applied
Microbiology
and
Biotechnology, 97: 9291-9321

Velho-Pereira, Sonashia Kamat, Nandkumar M (2013) Actinobacteriological research in India.
Indian Journal of Experimental Biology 51:573-596

Prakash, S. Ramasubburayan, R. Iyapparaj, P. Kumar, C. Mary, C. Jinitha. (2013) Screening
and partial purification of antifungal metabolite from Streptomyces rochei MSA14: an isolate
from marine mining soil of Southwest coast of India. Indian Journal of Geo-Marine
Sciences

Anne
A.
Madden
, Andrew
Grassetti
,Jonathan-Andrew
N.
Soriano,
Philip
T.
Starks. Actinomycetes with Antimicrobial Activity Isolated from Paper Wasp (Hymenoptera:
Vespidae: Polistinae) Nests. Environmental Entomology 42(4):703-710. 2013

Dong-sheng Wang, Quan-hong Xue, Yun-yan Ma, Xiao-li Wei, Jie Chen, Fei He (2013)
Oligotrophy is Helpful for the Isolation of Bioactive Actinomycetes Indian Journal of
Microbiology 10.1007/s12088-014-0444-1

Palaniappan Sivasankar*, Panchanathan Manivasagan, Packiyaraj Vijayanand, Kannan
Sivakumar,
Shanmugam
Sugesh,
Subramaniam
Poongodi,
Viswanathan
Maharani,
Shanmugam Vijayalakshmi, Thangavel Balasubramanian (2013) Antibacterial and brine
shrimp lethality effect of marine actinobacterium Streptomyces sp. CAS72 against human
pathogenic bacteria. Asian Pac J Trop Dis, 3(4): 286-293

Djinni, I., Defant, A., Kecha, M. and Mancini, I. (2014), Metabolite profile of marine-derived
endophyticStreptomyces
sundarbansensis
WR1L1S8 by
liquid
chromatography–mass
spectrometry and evaluation of culture conditions on antibacterial activity and mycelial growth.
Journal of Applied Microbiology, 116: 39–50. doi: 10.1111/jam.12360

B. Sadeghya* & N. Hatamia The effect of different conditions of temperature and acidity on
antimicrobial metabolites of Streptomyces sp. isolates C-11 and C-26 against F. subglutinans
Archives Of Phytopathology And Plant Protection DOI: 10.1080/03235408.2013.819655

Devi N D Asha; Pushpaltha, KC (2013) Monitoring and biocontrol of paddy fungal
pathogens collected from Hassan District. Journal of Biopesticides 6:6-13.
Projects I worked in:
1) Searching potential metabolite from Actinomycetes active against drug resistant bacterial
pathogens” funded by Uttarakhand council for Science and Technology.
Subjects Studied:
Chemistry, Botany, Zoology, Biotechnology, Medical Microbiology, Food Microbiology, Bacteriology,
Virology, Mycology, Phycology, Industrial Microbiology, Biochemistry & Microbial physiology,
Recombinant DNA Technology, Molecular biology & Microbial genetics, Immunology, Cellular
Microbiology, Soil Microbiology, Environmental Microbiology, Biostatics and Computer.
Teaching subjects: (B.Sc and M.Sc)
Food Microbiology, Industrial Microbiology, Food Additives, Enzymes in Food processing, Food
Biochemistry and Biotechnology, Fermentation technology, Biochemistry & Microbial physiology,
Recombinant DNA Technology, Molecular biology & Microbial genetics,
Techniques acquainted with:
1. Production, purification, identification, characterization of metabolites and enzymes from
microbial sources.
2. Protein purification techniques like Gel filtration, Ion exchange, PAGE (SDS denaturing, SDSnon denaturing, Native), Western blot etc
3. Agarose gel electrophoresis, Polymerase Chain Reaction
4. Enzyme assay techniques, plasmid isolation, UV- mutagenesis of microbes
5. Screening, isolation, identification and Maintenance of microbial cultures
6. Isolation, identification, purification and characterization of novel compounds.
7. Scanning Electron Microscopy.
8. UV-Visible spectrometry
9. Estimation of proteins, carbohydrates
10. Expertise in handling instruments as
centrifuge machine, speed vac concentrator, Gel
documentation system, UV-Trans illuminator, Multi Mode Microplate reader, compound
microscope, bioreactor
11. Competent cell preparation and transformation with recombinant DNA
12. 16S rRNA sequencing based identification of natural bacterial isolates and phylogenetic
analysis
Workshop attended
1. Participated in Workshop on “Bioinformatics in Molecular Modeling, Data Analysis and
System Biology” Biotech Park, Lucknow, Aug. 2008.
2. Participated in summer school on “Trends in Biology and Biotechnology”, Forest Research
Institute, Dehradun, Uttarakhand, sponsored by State Biotechnology Programme, Govt. of
Uttarakhand, dated 18th to 29 August 2008
3. Participated
Sensitization
workshop
on
Administrative
Responsibilities
for
the
Implementation of Scocietal Research Fellowship (SoRF)-DST, New Delhi; organized by
The National Academy of Sciences, India (NASI), on March 21, 2014 at Allahabad.
Achievements
Editorial board member in:

Executive editor in International Journal of Microbiology research p-ISSN: 0975-5276;
e ISSN:0975-9174

Associate editor in American Journal of Current Biology

Judge the students at 15th Uttarakhand Rajya Satirya Bal Vigyan Congress-2007 on theme –
“Biodiversity: Save nature-Save future” held at Haridwar, Uttarakhand
Work Experience:

Have experience of teaching Biotechnology (PG) to higher secondary classes.

Have worked as a Teaching Assistant for M.Sc. Microbiology in S.B.S.P.G.I.

Have done invigilation duty and Exam script correction for semester examinations in S.B.S. P.G.I
and BFIT

Have trained and assisted M.Sc. students, project staffs, B. Pharma students for their project
works.

Have done official duties for Department of Microbiology of S.B.S.P.G.I. & BFIT

Have done reviewer job for scientific articles published in National and International journals.
Personal Information:
Sex
:
Male
Address
:
C/O Sh. Kailash Semwal
Vill- Suddhowala
Post- Jhajra
Distt- Dehradun, Uttarakhand-248007
Email
:
[email protected]
Mobile no.
:
+91-7520613320
Qualification
:
B.Sc. Biology-2002
M.Sc. Microbiology-2006
PhD: Biotechnology-2013
D.O.B.
:
01/01/1981
Marital Status :
Unmarried
Nationality
:
Indian
Sex
:
Male
Languages known: English, Hindi, Punjabi, Bhojpuri
The above-furnished information is true to the best of my knowledge.
Place: Dehradun
Date: 1/03/2014
(Vijay Kumar)