Figure 3Three-photon optogenetic excitation at 1300 nm. (a) Representative examples of cultured neurons excited by three-photon excitation. The duration of exposure
is indicated by the shaded area. (b) An example of the response to different excitation frequencies (2, 5, 10 and 20 Hz in ascending order). Exposure is indicated by the
shaded area. (c) An illustration of the patched neuron with the three-photon excitation pattern superimposed. (d) Irradiance map of the spot, assuming 52 mW total
integrated power at the sample. Units of irradiance are W mm−2. (e) Comparison of the 470 nm one-photon (n=19) excitation probability and the 1300 nm three-photon
(n=9) excitation probability for cells expressing CoChR as a function of excitation frequency. Error bars indicate one standard deviation from the mean.
Christopher J Rowlands, Demian Park, Oliver T Bruns, Kiryl D Piatkevich, Dai Fukumura, Rakesh K Jain, Moungi G Bawendi, Edward S Boyden
Wide-field three-photon excitation in biological samples
null,2016,6(5)
http://dx.doi.org/10.1038/lsa.2016.255