Isolation of thermophilic aerobic bacteria with hydrolytic activities
from Algerian terrestrial hot environments
Mohamed Amine GOMRI
Department of Biotechnology, Institute of Nutrition and Food Technologies, University of Constantine, Algeria
Abstract
Thermophilic and hyperthermophilic microorganisms inhabiting hot environments such as terrestrial hot springs
have interesting molecular adaptation capacities and constitute an important source of bioactive molecules
emerging from unique molecular and biochemical mechanisms. Hydrolytic enzymes of these organisms offer
major advantages and give new possibilities to ameliorate existing processes or to create new ones.
With the purpose of studying some of their enzymatic potentials, a hundred of thermophilic, aerobic bacteria
strains were isolated from water and sediment samples taken from different points divided between the regions of
Guelma, Khanchela and Ouargla located in North-Eastern and South-Eastern of Algeria, respectively.
Phenotypic characterization has been realized, and allowed to obtain some morphological, biochemical and
physiological proprieties of these microorganisms, these data led to realize a dendrogram by numerical taxonomy.
Four types of hydrolytic activities were investigated by using seven different substrates.
Strains were generally rods and filaments, Gram positive in most cases; they were moderately thermophiles,
aerobes and neutrophiles. Numerical analyses clustred them into 3 principal phenons, based on similarity between
strains. Screening for hydrolytic activities led to find one or several types of hydrolases in seventy-seven strains,
proteolytic and amylolytic activities were mostly found.
Biography
Mohamed Amine GOMRI is preparing his PhD University of Constantine 1, Algeria. He is an assitant-techear and
researcher at the same university.
[email protected]
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