Supplementary Information
350000
250000
a
200000
AMP
100000
MET
150000
EPE
Fluorescence (a. u.)
300000
50000
0
0
2
4
6
8
10
12
14
16
18
20
Time (min)
700000
b
400000
300000
AMP
MET
500000
EPE
Fluorescence (a. u.)
600000
200000
100000
0
0
1
2
3
4
Time (min)
5
6
7
Figure S1. Chromatograms obtained for a standard solution containing AMP, MET and EPE
(8.0 µg/mL, each) using a percentage of acetonitrile in the mobile phase of: (a) 50 % and (b) 70 %,
using an unmodified TRB-35 extractive capillary (30 cm length) for IT-SPME. For other experimental
details, see text.
8
S2
1600000
Fluorescence (a. u.)
1400000
1200000
1000000
800000
600000
50 cm
400000
40 cm
200000
30 cm
0
0
2
4
6
8
Time (min)
Figure S2. Chromatograms obtained for a standard solution containing AMP, MET and EPE
(8.0 µg/mL, each) with TRB-35 extractive capillaries of different length. For other experimental details,
see text.
1400000
AMP
EPE
Fluorescence (a. u.)
1000000
MET
1200000
c
800000
600000
b
400000
200000
a
0
0
1
2
3
4
5
6
7
8
Time (min)
Figure S3. (a) Chromatograms obtained for the reaction mixture (250 µL of buffer and 250 µL of
0.1 mM FMOC), (b) the same reaction mixture after the introduction of a cotton swab for 5.0 min, and
(c) after the introduction of a cotton swab impregnated with 125 µL of a standard solution of EPE
(2.0 µg/mL). For IT-SPME a c-MWCNTs functionalized TRB-35 capillary (40 cm length) was used. For
other experimental details, see text.
S3
Table S1. Response vs. concentration linearity data.
Capillary
Linearity (y = ax + b)
Concentration
Interval (µg/mL)
EPE
AMP
MET
a = (39 ± 3) × 104
a = (606 ± 14) × 103
a = (159 ± 5) × 104
b = (−5 ± 2) × 106
b = (3 ± 9) × 105
b = (−8 ± 3) × 106
R2 = 0.980
R2 = 0.998
R2 = 0.997
a = (23 ± 7) × 104
a = (22 ± 9) × 104
a = (64 ± 8) × 104
b = (2 ± 5) × 106
b = (2 ± 6) × 106
b = (21 ± 14) × 105
TRB-5 c-SWCNTs
R2 = 0.764
R2 = 0.658
R2 = 0.962
L= 36 cm
a = (35 ± 4) × 104
a = (36 ± 8) × 104
a = (97 ± 14) × 104
b = (−27 ± 15) × 104
b = (9 ± 4) × 105
b = (11 ± 8) × 105
R2 = 0.987
R2 = 0.910
R2 = 0.96
a = (117 ± 4) × 103
a = (28 ± 2) × 104
a = (70 ± 5) × 104
b = (−3 ± 3) × 105
b = (11 ± 13) × 104
b = (6 ± 3) × 106
R2 = 0.997
R2 = 0.990
R2 = 0.983
a = (61 ± 17) × 103
a = (10 ± 3) × 104
a = (33 ± 9) × 104
b = (17 ± 11) × 105
b = (2 ± 2) × 106
b = (1 ± 6) × 105
R2 = 0.810
R2 = 0.750
R2 = 0.800
a = (8 ± 2) × 104
a = (15 ± 2) × 104
a = (49 ± 2) × 104
b = (8 ± 1) × 105
b = (3 ± 10) × 104
b = (−52 ± 10) × 104
R2 = 0.910
R2 = 0.972
R2 = 0.997
TRB-35 c-MWCNTs
L= 40 cm
2.0–10.0
2.0–10.0
2.0–8.0
TRB-35 L= 30 cm
2.0–10.0
2.0–10.0
TRB-5 L= 30 cm
2.0–8.0