Surface plasmon resonance
Figure1. The configuration of an SPR
[1]
chip
Figure2. The workings of the SPR
[1]
chip
[1] Sabban, Sari. Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high- affinity FcεRI
receptor (PhD thesis), (2011) The University of Sheffield
History
• Woods explaining the concept of surface plasma
[2]
excitation in gratings in 1902 .
• Kretschmann et al. proposed the practical means of
excitation of SPR at the metal–dielectric interface in the
[3]
prism based configuration in 1968 .
• The first major application work utilizing SPR was
[4]
reported in 1983 by Liedberg et al .
[2] R.W. Wood, On a remarkable case of uneven distribution of light in a diffraction grating
spectrum, Phil. Mag. 4 (1902) 396–402.
[3] E. Kretschmann, H. Raether, Radiative decay of non-radiative surface plasmon excited by
light, Z. Naturforsh 23A (1968) 2135–2136.
[4] B. Liedberg, C. Nylander, I. Lundstr¨om, Surface plasmon resonance for gas detection
and biosensing, Sens. Actuators B 4 (1983) 299–304.
Kretschmann configuration
Fig. 3. Three-layer Kretschmann
[5]
configuration for SPR excitation
Here, k0 =2π/λ0 is the wave vector in free
space, λ0 the freespace wavelength, npthe
refractive index of the prism, ns the fractive
index of the dielectric sample, and εm the
[5]
complex dielectric constant of the metal .
[5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface
plasmon resonance sensor. Sensors and Actuators B 122 (2007) 381–388.
SPR setup
Fig. 4. Schematic of prism-based SPR setup
working in angular interrogation mode. LD:
laser diode(670nm); P: polarizer; PD:
[5]
photodiode; ADC: analog-to-digital converter .
[5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface plasmon
resonance sensor Sensors and Actuators B 122 (2007) 381–388.
Result
Fig.6. Light reflection in the
Kretschmann geometry of the
ATR method. Assuming the
structure prism–metal–
dielectric in the y-z plane is
infinite
Fig. 5. Experimental and theoretical SPR reflection
spectra. Dots for experimental spectra and solid
line for theoretical spectra.
2
反射率R=|rpmd|
Fig.7 Log Log plot of
n(solid line) and k(dash
line) vs wavelength for
silver【6】
[6] David W. Lynch and W. R. Hunter.
350-357
Theoretical SPR reflection curves
Fig. 8. SPR reflection curves as functions of incident angle
obtained using Fresnel formulae for two different dielectric media
and three different prism materials: (a) np = 1.597, (b) np =
1.51391 and (c) np = 1.456. Curves A, C, and E are for ns = 1.33,
and curves B, D, and F are for ns = 1.36 [5].
[5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface
plasmon resonance sensor Sensors and Actuators B 122 (2007) 381–388.
Fig. 9. Gold: -e,(w) and e2(W) vs frequency. The solid line is the
Drude model. The data from Ref. 8 are: Bennett and Bennett, *
for both -el and e2; Schulz, 0 for both; Motulevich and Shubin, for
both; Padalka and Shklyarevskii, 0 for both; Bolotin et al., x for both;
Brandli and Sievers, + for both; Weaver et al., A for both.[7].
[7] M. A. Ordal, L. L. Long, R. J. Bell, S. E. Bell, R. R. Bell, R. W. Alexander, Jr., and C. A. Ward.Optical
properties of the metals Al, Co, Cu, Au, Fe, Pb, Ni, Pd,Pt, Ag, Ti, and W in the infrared and far infrared.
APPLIED OPTICS 22 (1983) 1099-1120.
[8]Au: H. E. Bennett and J. M. Bennett, Optical Properties and Electronic Structure of Metals and Alloys, F. Abeles, Ed.(North-Holland, Amsterdam, 1966), p. 75; L. G. Schulz, J. Opt. Soc. Am. 44,
357,362 (1954); G. P. Motulevich and A. A. Shubin, Sov. Phys. JETP 20, 560 (1965); V. G. Padalka and I. N. Shklyarevskii, Opt. Spectrosc. 11, 285 (1961); G. A. Bolotin, A. N. Voloshinskii, M. M.
Kirilbra, M. M. Neskov, A. V. Sokolov, and B. A. Charikov, Fiz. Met. Metalloved. 13, 823 (1962); G. Brandli and A. J. Sievers, Phys. Rev. B 5, 3550 (1972).
Experimental SPR reflection spectra
Fig. 10. Experimental SPR reflection spectra for water and ethanol
solutions with concentrations of 5, 10, 15, 20 and 25 wt.% [5]
Fig. 11. Plot of numerically generated resonance angle as
function of refractive index of dielectric medium obtained for
three different prism materials. The slope of each line
indicates the angular sensitivity [5].
[5] Gaurav Gupta, Jun Kondoh .Tuning and sensitivity enhancement of surface plasmon resonance sensor
Sensors and Actuators B 122 (2007) 381–388.
用表面等离子体共振传感器测量
金胶体溶液的折射率
图12
[9]
SPR传感器系统结构示意图
[9]西安交通大学物理教学实验中心特色实验项目
[9]
图13浓度不同的金溶胶样品SPR角谱实验曲线
实验内容
• 1. 测量三棱镜折射率n(𝛌)。
• 2. 测量液体（如生理盐水、酒精）的折射率ns。
• 3. 测量不同折射率ns时对应共振角𝛉sp。
• 4. 计算金属膜的介电常数。
购买光谱仪
• 添加购置天津港东的WGD-3型光栅光谱仪三
台用于物理实验（上）以及配置3台电脑