Supplementary Information
Supplementary Figure 1. Live cell imaging of cyclin B degradation (blue, BG430) and protein
expression (green, YFP) in unperturbed cells +/- Cdh1kd. mCherry-tagged histone H2 is used to
visualize the chromosomes.
Supplementary Figure 2. Addition of Cdk1 inhibitor RO-3306 could not restore diminished
loading of MCM4 in Cdh1-kd cells released from a nocodazole-block.
Supplementary Figure 3. Live cell imaging of a normal centrosome (green dots, GFP) cycle in
U2OS (video 12). RFP-tagged histone H2 is used to visualize chromosomes.
Supplementary Figure S1. Live cell imaging of cyclin B degradation (blue, BG430) and protein
expression (green, YFP) in unperturbed cells +/- Cdh1kd. mCherry-tagged histone H2 is used to
visualize the chromosomes.
Supplementary Figure S2. Live cell imaging of a normal centrosome (green dots, GFP) cycle
in U2OS (video 12). RFP-tagged histone H2 is used to visualize chromosomes.
Supplementary Video S1. S-Phase duration measured by EGFP-PCNA. U2OS cells were
transduced with H2B-RFP (red) and EGFP-PCNA (green). Images were acquired every 10 min
for 16 h on an Olympus IX-81 inverse microscope.
Supplementary Video S2. Mitotic arrest upon treatment with the Plk1-Inhibitor BI6727.
U2OS cells were transduced with H2B-GFP (green) and mCherry-Tubulin (red). Images were
acquired every 10 min for 14.5 h on an Olympus IX-81 inverse microscope.
Supplementary Video S3. Development of 53BP1 foci following mitotic exit. U2OS cells
were transduced with H2B-RFP (red) and transfected with EGFP-53BP1 (green). Images were
acquired every 10 min for 15 h on an Olympus IX-81 inverse microscope.
Supplementary Video S4. Lagging chromosomes and apoptosis during mitosis. U2OS
cells were transduced with H2B-RFP (red). Images were acquired every 10 min for 16.5 h on an
Olympus IX-81 inverse microscope.
Supplementary Video S5. Tripolar mitosis and anaphase bridge. U2OS cells were
transduced with H2B-RFP (red). Images were acquired 5 min for 13.5 h on an Olympus IX-81
inverse microscope.
Supplementary Video S6. Anaphase bridge and micronuclei. U2OS cells were transduced
with H2B-RFP (red). Images were acquired 5 min for 13 h on an Olympus IX-81 inverse
microscope.
Supplementary Video S7. Multipolar mitosis and cell fusion. U2OS cells were transduced
with H2B-RFP (red) and transfected with EGFP-MyrPalm (green). Images were acquired every
10 min for 14.5 h on an Olympus IX-81 inverse microscope.
Supplementary Video S8. Cytokinesis defect. U2OS cells were transduced with H2B-RFP
(red) and transfected with EGFP-MyrPalm (green). Images were acquired every 10 min for 15 h
on an Olympus IX-81 inverse microscope.
Supplementary Video S9. Apoptosis in mitosis. U2OS cells were transduced with H2B-RFP
(red) and transfected with EGFP-MyrPalm (green). Images were acquired every 10 min for 14.5
h on an Olympus IX-81 inverse microscope.
Supplementary Video S10. Supernumerary centrosomes. U2OS cells were transduced with
H2B-RFP (red) and transfected with Centrin-EGFP (green). Images were acquired every 10 min.
for 28 h on an Olympus IX-81 inverse microscope.
Supplementary Video S11. Cell fusion and centrosome clustering. U2OS cells were
transduced with H2B-RFP (red) and transfected with Centrin-EGFP (green). Images were
acquired every 10 min. for 45 h on an Olympus IX-81 inverse microscope.
Supplementary Video S12. Normal centrosome cycle. U2OS cells were transduced with
H2B-RFP (red) and transfected with Centrin-EGFP (green). Images were acquired every 15 min.
for 40 h on an Olympus IX-81 inverse microscope.