University of Massachusetts Lowell The Department of Electrical and Computer Engineering Subject: Introduction to Biosensors Wireless Optical Sensor Network Project Report Two Team members: Ravi G Bhatia Wenchan Tong Aiken Pang Report Submit Date: 11th April, 2007 Project Overview: Fig 1. Wireless Sensor Project This project is about building a wireless sensor that transmits information obtained from 1550nm wavelength Fiber optic cavity wirelessly using a photodetector and a wireless transceiver module to a computer for processing Biomolecular interactions. Sensor Head is an optical cavity such as a Fabry Perot cavity. Biomolecular interaction changes optical path length. Intensity change is proportional to optical path length and as the path length changes photodetector will detect change in intensity and transmit that information through the wireless module. Fig2 Light Intensity before (original) and after biomolecular interaction Progress Made: We have purchased a photodetector for our project. It is a InGaAs pin photodetector with responsivity from 800nm to 1700nm sitable for our project. The photodetector has a fiber pigtail connector for directly connecting with the fiber sensor tip. Cost of the detector is $269. Fig3: Spectral reponse of DET01FC InGaAs pin photodetector. Reference: “DET01CFC Operating Manual — Fiber Input InGaAs Photodetector” Photodetector Circuitry 3. Fig4: schematic of the photodetector circuit. When light of intensity P is incident on the photodetector, an output voltage appears across the load as shown in fig 2 which is proportional to the intensity of incident light give by. Voltage=P * Responsivity * RL The Responsivity and RL are constants for a given circuit. Responsivity = 0.95 A/W (from datasheet) RL = 50 ohms (recommended by Thorlabs; Ref. datasheet DET01FC) Therefore, The output voltage in our circuit is equal to Voltage = 0.95 * 50 *Intensity = 47.5 * Intensity The voltage across the load resistor is coupled to the wireless module circuit which converts it into digital signal and transmits the signal to a computer for detecting whether the biomolecular interaction has taken place. But first, The wireless module receiver has to be modified to accept the voltage corresponding to a particular light intensity and Convert it into appropriate digital data via the built in ADC. Next the computer software that will decode data and process whether interaction has taken place has to be calibrated for identifying light intensity before the interaction. And the expected intensity change after interaction. Other information such as the extent of the intensity change which might give some information about the concentration of the biomolecules might also have to be programmed into the system Experimental Setup: Initial measurements were carried out using a 1550nm Tunable Laser Source as shown in Fig 5. Laser light of -3dbm power is coupled to optical fiber cable onto an optical cavity show in Fig 6. Reflections form the optical cavity are detected using the purchased photodetector. Biomolecular interaction is simulated by reducing the length of the cavity so the the intensity of light detected by the photodetector changes. Fig. 5 1550nm Tunable Laser Source Fig. 6 Optical Cavity for simulating Biomolecular Interaction Experimental observations In this initial experiment, light reflected form the optical cavity and detected by the purchased detector was observed on digital oscilloscope. For a Load resistance of 20KΩ, the following output was observed as shown in Fig 7 and Fig 8. No output was obtained for a 50Ω resistance recommended by Thorlabs due to the very weak intensity light reflected by optical cavity. Fig. 7 Output before simulated interaction Fig. 8 Output after simulated interaction Experimental Results Results of this initil experiment are tabulated below. Incident Power -3dbm or 0.5mW @ λ=1550nm Photodetector output 44mV Before Biomolecular interaction (simulated) Photodetector output 101mV After Biomolecular interaction (simulated) Voltage change 57mV Table 1: Experimental Results Observations Output voltage is noisy and fluctuates 20-30mV. Noise is superimposed in the signal due vibrations in the cavity or due high load resistance – 20K Ω. Recommended resistance is 50 ohms but photodetector output is too weak to detect with 50ohms. This setup cannot be used to detect weak intensity light and transmit information reliably over the network. One possible solution of interest is to use a Transimpedance Amplifier which will amplify the photodetector signal and give better noise performance. Power amplifier Since the range of output voltage of photodetector is 20-30mv which is not large enough to convert by ADC, a power amplifier is needed to amplify input output the dynamic range of the output voltage. min 20 mv 1v max 30 mv 2v The maximum input of ADC is 2.5v, so the desirable dynamic range of the output of amplifier is 1v-2v. Operational amplifier Among varieties of amplifiers, operational amplifier is a solid state integrated circuit amplifier which employs external feedback for control of its transfer function or gain. 741 chip 741 is a classic integrated circuit operational amplifier chip, made by virtually all IC manufacturers. It is one of the most well know examples of the most versatile electronic building blocks ever created. Because of its good performance, stable function, easily used and cheap price, 741 is used in this project as the power amplifier. Circuit of amplifier V+ = Vin V− = K Vout Vout = G(Vin − K Vout) Vout/Vin = G /(1 + G K) If G is very large, Vout/Vin comes close to 1 + (R2/R1) Parameter: R2=2KΩ , R1=1KΩ So that we can get the gain close to 3 Problems and solutions 1. the fiber needed was broken in the lab 2. output signal of photodetector cannot be the one need for the next process of the project: -The output signal of photodetector below 1V -The signal is DC plus AC rather than just only DC needed For the first problem, an optical fiber cable is made to use. For the second problem, three methods have been tried. 1) Multi-meter: still cannot solve the problem of DC plus AC 2) Analog oscilloscope: has no function of sampling 3) Digital oscilloscope: can solve both problems Wireless Sensor Network (WSN) module ADC In the WSN module, the ADC is a Sample and Hold 12 bits converter. Sample and hold type ADC can provide faster sampling rate then other types (e.g. SigmaDelta). The sampling speed in our module can be 6.5MHz/17 = 382ksps. We use the internal reference voltage for the ADC. The reference voltage can be 1.5V or 2.5V. We will use 2.5V for the prototype. The resolution is equal to (2.5v)/ (2^12) = 0.61mV. NADC is the output digital value of the ADC. Software platform TinyOS was initially developed by the U.C. Berkeley EECS Department. TinyOS is an event based operating system. Tradition OS is using time base. For example Window is a time base. Event based mean every operation in the system is base on event. Events in the WSN are send data, request sensor data. This kind of design can support networked sensors with minimum hardware implementation (fewer timers). Programming Language The language using in TinyOS is “NesC”. It likes C. The NesC has a custom complier. It is used to translate Nesc to C. Timetable WEEK TASK PLAN March 21 - 27 Simulate Biosensor operation for obtaining intensity change March 28 - 31 Finding a correct Photodetector April 2-6 Order InGaAs APD April 9 - 11 Testing InGaAs APD under simulation of Biosensor spec. April 12 - 15 Circuit design, Programming wireless module April 16 - 21 Hardware Assembly and Testing We did fine-tune the timetable because we got the InGaAs APD on 4/6/07(not 3/25/07). We swap the time for simulate biosensor operation with Order InGaAs APD. Budge Wireless sensor node: US$100 each (we need at least two) Photosensor: US$265 each Total cost will be $365
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