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NEBNext® Multiplex Oligos for Illumina® (Dual Index
Primers Set 1)
Catalog #
E7600S
Size
96 reactions
Concentration
Categories: ChIP-Seq Library Prep, DNA Library Prep, FFPE DNA | More +
Applications: DNA for Illumina®, Illumina® Library Preparation
Product
Information
FAQs &
Tech Tips
Description
Protocols &
Manuals
Quality &
Safety
Legal
Information
Properties and Usage
Description
The NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) contains the adaptor and index primers that are ideally suited for
multiplex sample preparation for next-generation sequencing on the Illumina platform. Each of these components must pass rigorous quality
control standards and are lot controlled, both individually and as a set of reagents.
Dual Index Workflow
Lot Control
The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme
activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component
page
Reagents Supplied
The following reagents are supplied with this product:
Store at (°C)
NEBNext Adaptor for Illumina
-20
USER® Enzyme
-20
NEBNext i501 Primer
-20
NEBNext i502 Primer
-20
NEBNext i503 Primer
-20
NEBNext i504 Primer
-20
NEBNext i505 Primer
-20
NEBNext i506 Primer
-20
NEBNext i507 Primer
-20
NEBNext i508 Primer
-20
NEBNext i701 Primer
-20
NEBNext i702 Primer
-20
NEBNext i703 Primer
-20
NEBNext i704 Primer
-20
NEBNext i705 Primer
-20
NEBNext i706 Primer
-20
Concentration
NEBNext i707 Primer
-20
NEBNext i708 Primer
-20
NEBNext i709 Primer
-20
NEBNext i710 Primer
-20
NEBNext i711 Primer
-20
NEBNext i712 Primer
-20
Properties and Usage
Materials Required but not Supplied
Required Materials Not Included for DNA or ChIP Libraries:
Enzymes and buffers appropriate for DNA or ChIP Illumina library preparation. (DNA: #E7645, #E7370, #E6040, #E6000 ChIP:
#E7645, #E7370, #E6240, #E6200)
Agencourt® AMPure® XP Beads (Beckman Coulter, Inc. #A63881)
Magnetic Stand
DNA LoBind® Tubes (Eppendorf®) / PCR Tubes/96-well PCR Plates
Freshly prepared 80% Ethanol
Nuclease-free Water
0.1X TE (or 10 mM Tris-HCl, pH 7.5–8.0)
10 mM Tris-HCl, pH 8.0
10 mM NaCl
Bioanalyzer® (Agilent Technologies, Inc.)
Bio-Rad® Microseal® (Bio-Rad Laboratories, Inc.) "A" Film or equivalent
Recommended products not supplied:
TruSeq® Index Plate Fixture (Illumina #FC-130-1005) or similar setup
Required Materials Not Included for RNA Libraries:
Enzymes and buffers appropriate for RNA Illumina library preparation. (#E7420, #E7530, #E6110, #E6100)
NEBNext Poly(A) mRNA Magnetic Isolation Module (#E7490), or NEBNext rRNA Depletion Kit (Human, Mouse, Rat) (#E6310 ) or similar
Magnetic Rack (Alpaqua, cat #A001322 or equivalent)
80% Ethanol (freshly prepared)
0.1X TE (or 10 mM Tris-HCl, pH 7.5–8.0)
10 mM NaCl
Agencourt AMPure XP Beads (Beckman Coulter, Inc. #A63881)
Actinomycin D (Sigma# A1410, dissolved in dimethylsulfoxide [DMSO] to 5 μg/µl).
DNA LoBind Tubes/PCR Tubes/96-well PCR Plates
Bioanalyzer (Agilent Technologies, Inc.)
Bio-Rad Microseal (Bio-Rad Laboratories, Inc.) "A" Film or equivalent
Recommended products not supplied:
TruSeq Index Plate Fixture (Illumina #FC-130-1005) or similar setup
Additional materials required for use with #E6110 and #E6100:
3 M Sodium Acetate, pH 5.5
100% Ethanol
PCR Column Purification Kit (Qiagen or other)
DNA Gel Extraction Column Purification Kit
RNeasy® MinElute® Clean Up Kit (Qiagen® #74204)
Storage Temperature
-20°C
FAQs
FAQs
1.
2.
3.
4.
5.
Do NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)come with library prep reagents?
Can I use NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) for library prep from both DNA and RNA samples?
How many libraries can I prepare with the NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1)?
For pooling <96 samples, what combination of indices should I use?
Do I need to spike in custom sequencing primers when sequencing libraries made with NEBNext Multiplex Oligos for Illumina
6.
7.
8.
9.
(Dual Index Primers Set 1) on the Illumina platforms?
Can I perform single read runs and still get both index sequences? *
Can I run both single read and paired-end recipes with dual-indexed libraries? *
How does dual indexing work? *
Is the USER® enzyme in our NEBNext kits identical in concentration to the standalone product?
Protocols
Manuals
Protocols
1. Setting up the PCR Reaction - NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (E7600)
Manuals
The Product Manual includes details for how to use the product, as well as details of its formulation and quality controls. The
following file naming structure is used to name these document files: manual[Catalog Number].
manualE7600
Safety Data Sheet
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
NEBNext Adaptor for Illumina
USER® Enzyme
NEBNext i501 Primer
NEBNext i502 Primer
NEBNext i503 Primer
NEBNext i504 Primer
NEBNext i505 Primer
NEBNext i506 Primer
NEBNext i507 Primer
NEBNext i508 Primer
NEBNext i701 Primer
NEBNext i702 Primer
NEBNext i703 Primer
NEBNext i704 Primer
NEBNext i705 Primer
NEBNext i706 Primer
NEBNext i707 Primer
NEBNext i708 Primer
NEBNext i709 Primer
NEBNext i710 Primer
NEBNext i711 Primer
NEBNext i712 Primer
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.