PE anti-human Ig light chain κ Antibody

Version: 3
Revision Date: 01/22/2016
PE anti-human Ig light chain κ Antibody
Catalog# / Size
316508 / 100 tests
316507 / 25 tests
Clone
MHK-49 (See other available formats)
Other Names
Immunoglobulin light chain kappa
Isotype
Mouse IgG1, κ
Ave. Rating
★★★★★ 0 reviews
Description
The MHK-49 antibody reacts with both soluble and membrane human
immunoglobulin light chain kappa (κ). It does not react with human
immunoglobulin light chain lambda (λ) or heavy chain. The MHK-49
antibody can be used as primary or secondary reagent for
immunofluorescent staining or ELISA analysis.
Reactivity
Human
CD19+ human B-lymphocytes stained with
MHL-38 (anti-λ) FITC and MHK-49 (anti-κ) PE
See PE spectral data
Immunogen
Human Ig cocktail
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA
(origin USA).
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal
conditions. The solution is free of unconjugated PE and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our
Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application
FC - Quality tested
Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow
cytometric analysis. Test size products are transitioning from 20 µl to 5 µl per test. Please
check your vial or your CoA to find the suggested use of this reagent per million cells in 100 µl
staining volume or per 100 µl of whole blood. It is recommended that the reagent be titrated for
optimal performance for each application. Read more at www.biolegend.com/testsize regarding
the test size change.
Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References
1. Lockridge JL, et al. 2013. Biol. Blood Marrow Transplant 9:1310-22. (ELISA)
(PubMed link indicates
BioLegend citation)
Publication Library
RRID
AB_493613 (BioLegend Cat. No. 316508)
AB_493612 (BioLegend Cat. No. 316507)
Antigen Details
Structure
Ig family
Distribution
B cells
Gene ID
3514
3514
UniProt
Documentation
View information about Ig light chain kappa on UniProt.org
Technical data sheet
Certificate of Analysis
Safety Data Sheet
Related Protocols
Cell Surface Immunofluorescence Staining Protocol
Related FAQs
There are no FAQs for this product.
Related Categories
Antibodies and More > Secondary Reagents > anti-human Ig light chain κ > MHK-49 >
Immunology > Human Immunology > CD and Related Molecules > Ig light chain κ > MHK-49 >
Immunology > Human Immunology > B Cells > Ig light chain κ > MKH-49 >
Antibodies and More > Secondary Reagents > anti-human Ig light chain κ >
Other Formats
Description
Clone
Applications
Purified anti-human Ig light chain κ
MHK-49
FC, ELISA
Biotin anti-human Ig light chain κ
MHK-49
FC, ELISA
FITC anti-human Ig light chain κ
MHK-49
FC
APC anti-human Ig light chain κ
MHK-49
FC
Alexa Fluor® 488 anti-human Ig light chain κ
MHK-49
FC
Alexa Fluor® 647 anti-human Ig light chain κ
MHK-49
FC
PerCP/Cy5.5 anti-human Ig light chain κ
MHK-49
FC
Pacific Blue™ anti-human Ig light chain κ
MHK-49
FC
PE/Cy7 anti-human Ig light chain κ
MHK-49
FC
APC/Cy7 anti-human Ig light chain κ
MHK-49
FC
Brilliant Violet 421™ anti-human Ig light chain κ
MHK-49
FC
Purified anti-human Ig light chain κ (MaxPar® Ready)
MHK-49
FC, CyTOF®
Alexa Fluor® 700 anti-human Ig light chain κ
MHK-49
FC
For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or
other violations that may occur with the use of our products.
*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website,
www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or
used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties
without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited
Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or
animal diagnostic, therapeutic or commercial use.
BioLegend Inc., 9727 Pacific Heights Blvd, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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