Supplemental data
Table S1. Genes encoding major enzymes involved in antioxidant catabolism, and the primers
used for RT-PCR
Figure S1. Effects of reductant and PAO on the expressional regulation of stress-responsive genes.
Leaves were treated with 5 mM cysteine (A) or 50 M PAO (B) for 0.5 h, then dehydrated to 70%
of their fresh weight and incubated for 3 h, after which the expression of representative
stress-responsive genes was analyzed using RT-PCR. For each gene, the left lane is the control,
with no chemical treatments, and the right is the chemical treatment; M, weight makers.
(ACT2: forward TGGTACCGGTATGGTGAAGGCT, reverse CAAACGAGGGCTGGAACAAG
AC;COR47: forward ACCAGCGACGACAACAG GGTT, reverse TTCTCCTCCGGATGTTCC
ACTG;ADH1: forward CCACGTATCTTCGGCCATGAAG, reverse CTTCCGGTGCATTCCA
CACTC;RD29B: forward GGCGCACCAGTGTATGAATCCTC, reverse CTGATCATGAGT
TCCCGTCGGT; AAO3: forward CGATCAGGGCGATAGAAGTGGAG, reverse GTCTTCGTC
TGGCAAGGCAAGTG;ATHB-7: forward GCAGAGCCATTCTTGACCATG, reverse GCCAA
GGGTAATTGTTGCTGGAT; LTI30: forward AATCAAACCGGAGTGCAAAAG, reverse GG
TGAACAACGCCAGTATTACC;ABA3:forward CGCTGAAGCTTGTCGGAGAGACT, reverse
TCCAAGTAGCCAAACCAAGAGCC; ERD2: forward TGCACCTTGCCTCTCTTTCTCTC,
reverse CGGAAGCTTAAGTTTGGTGTTGG; ERD10: forward GAGGAATCATCGGCGCC
AGAGAT, reverse AGCGGTGTGGTGTTGACGACTTG; RD29A: forward GTACCGGAGATT
GCTGAGTCTT, reverse ACCGGAGTCAACTTCTCATCAAC; RD19: forward CTCGGTTTC
GTCGTCTGATGTC, reverse CTTGAACCTAGCCGGAGCGTAAC; RD2: forward TTGCCG
GAGGATGAAGAGTACAG, reverse CCAGCTTCTTTTCCAGGAACGAT)
Figure S2. Characteristic analysis of the chromatography-purified PTPase. Activity of the
chromatography purified PTPase was determined using [pTyr1018]-EGF receptor as a substrate as
described in the Materials and Methods. Various effectors were either added or not into the
reaction buffer. The values are expressed as a percentage of the activity with no effector added
into the reaction buffer. Values are means  SE of four samples.
Figure S3. Analysis of ZmRIP1 expression in yeast. (A) SDS/PAGE analysis of the expressed
ZmRIP1. Key: M, molecular weight maker; 1, crude extract of transformed yeast cells cultured in
SC-URA/galactose; 2, crude extract of transformed yeast cells cultured in SC-URA/glucose; 3,
elution of nickel affinity column for extract of yeast cells cultured in SC-URA/galactose; 4,
control (i.e. elution of nickel affinity column for extract of yeast cells transformed with an empty
p426GAL1 vector and cultured in SC-URA/glucose). (B) Effect of DTT on the expressed ZmRIP1
activity in yeast with or without galactose induction. Expressed ZmRIP1 was extracted and
assayed as described in the Materials and Methods. Yeast cells were cultured either in
SC-URA/galactose (galactose inducing) or SC-URA/glucose medium (glucose-cultured control).
ZmRIP1 activity was assayed either for crude extract or for elution through nickel affinity column.
‘+’ and ‘–’ denotes whether a corresponding event exits for a single assay or not, respectively.
Values are means  SE of four samples.
Figure S4. Characterization of ZmRIP1 transgenic seedlings. Wild-type Arabidopsis thaliana
(ecotype Col-o) was transformed with estradiol-inducible pER8-ZmRIP1 as described in the
Materials and Methods. (A) ZmRIP1 expression was evaluated using RT-PCR in different
transgenic lines treated with 10 mol/l estradiol for 24 h. Numbers below each lane indicate
corresponding lines. Key: c, non-transgenic control. (B) ZmRIP1 expression in line 1 treated with
different concentrations of estradiol for different length of time.
Figure S5. Expressional analysis of a range of genes encoding antioxidant enzymes in ZmRIP1
transgenic seedlings. Wild-type Arabidopsis thaliana (ecotype Col-o) was transformed with
estradiol-inducible pER8-ZmRIP1 as described in the Materials and Methods. Gene expression
was evaluated with RT-PCR in wild-type or transgenic seedlings of line 1 with (+) or without (-) 5
μmol/l estradiol treatment.