Reduced and oxidized gluthathione
Application Note
BioPharma
Authors
Introduction
Agilent Technologies, Inc.
HPLC separation of two glutathiones takes four minutes with Agilent Pursuit C18
Conditions
Column
: Agilent Pursuit C18, 4.6 mm x 150 mm, 5 μ
Part No.: A3000150X046
Mobile Phase
: H2O + 0.1% TFA, pH 2.0:CH30H- 90:10
Flow Rate
: 1.0 mL/min
Temperature
: Ambient
Detection
: 215 nm
Peak identification
1. L-Glutathione Reduced (γ-Glu-Cys-Gly)
2. L-Glutathione Oxidized (γ-Glu-Cys-Gly) (γ-Glu-Cys-Gly)
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This information is subject to change without notice.
© Agilent Technologies, Inc. 2011
Printed in the USA
31 October, 2011
First published prior to 11 May, 2010
A02300