OXIDATIVE STRESS MARKERS IN FOLLICULAR
FLUID OF YOUNG WOMEN WITH LOW RESPONSE
IN OVARIAN STIMULATION DURING IN VITRO
FERTILIZATION COMPARED TO FERTILE OOCYTE
DONORS.
R. NUÑEZ-CALONGE1, S.CORTES1, P.CABALLERO1,
E.VARA2, R. KIREEV2, J.FERNANDEZ-TRESGUERRES2.
1 Clínica Tambre, Madrid
2 Dpto. Fisiología, Universidad Complutense Madrid
Introduction
Introduction
Introduction
There are not enough studies analyzing the
relationship between OS and reduced
ovarian reserve in women undergoing
gonadotropin stimulation.
Hypothesis
One of the causes that could lead to low
ovarian reserve in young women could
be oxidative stress.
Objective
To determine the levels of oxidative stress
markers, antioxidant enzymes and cytokines in
the follicular fluid (FF) of young women with
low response in controlled ovarian stimulation
cycles compared with fertile oocytes donors, to
assess the oxidative stress impact on ovarian
reserve .
Methods
30 healthy,
fertile, Donors
(< 35 years old)
COS
rFSH+ antagonist GnRH
25 low
responders
Patients
(< 35 years old)
Methods
2.900 rpm, 10´
•Antioxidant enzymes : Glutathione peroxidase (GPx)
Glutathione transferase (GST)
•Oxidative stress markers: Malondialdehide (MDA)
•Inflammatory markers: Interleukine 6 (IL-6)
Interleukine 8 (IL 8)
Tumoral necrosis factor alpha (TNF α)
Vascular Endotelial Growth Factor (VEFG)
Results
Results
Antioxidant enzymes
p<0.05
Results
Antioxidant enzymes
NS
Results
Oxidative stress marker
p<0.01
Results
Inflammatory markers
p<0.001
Results
Inflammatory markers
NS
Results
Inflammatory markers
NS
Results
Inflammatory markers
NS
Summary
• The antioxidant GST has been found to be decreased in patients with
low response to COS.
•MDA, as one of the most important markers of OS, as well as the
proinflammatory cytoquine IL-6, have been found to be increase in
patients with reduced ovarian reserve.
•However, the relations of some antioxidant enzymes like GPx, and
cytokines (IL-8, TNF-alfa and VEGF) with OS and its implication in
ovarian reserve needs to be addressed.
Conclussions
•This is the first study analysing the relationship between oxidative
stress and low response in young patients undergoing controlled
ovarian stimulation cycles compared with fertile oocytes donors. The
results show that the most plausible explanation for low
response in young women is an increase in oxidative stress.
•Our results demonstrate that different levels of OS markers, oxidant
enzymes and cytokines in low responder patients as compared to
oocyte donors may have a negative impact on ovarian response.
Conclussions
A better knowledge of these alterations
could lead to the development of specific
antioxidant therapies for women with
poor
prognosis
undergoing
IVF
treatment.
THANK YOU FOR YOUR
ATTENTION