CHM 8304 E
ENZYME KINETICS
Winter 2014
Prof :
Office:
Tel :
E-mail :
Web site:
Jeffrey W. Keillor
D’Iorio 310
(613) 562-5800 extension: 6314
[email protected]
*Please include CHM 8304 in the subject line of all e-mail! *
http://mysite.science.uottawa.ca/jkeillor/English/Teaching.html
*Homework and course notes will be posted at this address.*
Course schedule
Wednesday, 10h00 – 11h30 (FSS 14001)
Friday, 10h00 – 11h30 (CBY E015)
Availability
Please feel free to make an appointment by e-mail, taking care to indicate CHM 8304 in
the subject line. If there is significant demand during the semester, I will set office hours.
EVALUATION
Assignments: 20% (Due one week after each module.)
Four take-home assignments of kinetic analysis.
Mid-term exam: 30% (Saturday, 1 February 2014)
Covering material up to and including Enzyme Kinetics module.
Final exam (cumulative): 50% (Wednesday, 19 February 2014)
Written exam based on all course notes.
ii
Syllabus
1. Enzyme catalysis
a. Enzymes and non-bonding interactions (review)
b. Catalysis (review)
c. Enzyme catalysis and energy diagrams
2. Experimental kinetics
a. Rate vs rate constants
b. Rate laws and molecularity
c. Practical kinetics
d. Kinetic analyses (simple equations)
e. Kinetic analyses (complex reactions)
3. Enzyme kinetics
a. Steady State Approximation
b. Saturation kinetics
c. Pre-steady state kinetics
d. Graphical representations
4. Enzyme inhibition
a. Reversible inhibition, modes of inhibition
b. Slow and/or tight-binding inhibition
c. Irreversible inhibition, affinity markers, suicide inhibition
5. Enzyme kinetics case studies (if time permits)
a. “Perfect” enzymes
b. Chymotrypsin mechanism
c. TS analogue inhibitors
d. Suicide inhibitors
Recommended reading (available in library)
Modern Physical Organic Chemistry, Anslyn & Dougherty
Fundamentals of Enzyme Kinetics, Cornish-Bowden
Enzyme Kientics: Behavior and Analysis of Rapid Equilibrium and Steady-State Systems, Segel