AKAP150 Contributes to Enhanced Vascular Tone by
Facilitating Large-Conductance Ca2+-Activated K+ Channel
Remodeling in Hyperglycemia and Diabetes
MellitusNovelty and Significance
by Matthew A. Nystoriak, Madeline Nieves-Cintrón, Patrick J. Nygren, Simon A.
Hinke, C. Blake Nichols, Chao-Yin Chen, Jose L. Puglisi, Leighton T. Izu, Donald M.
Bers, Mark L. Dell’Acqua, John D. Scott, Luis F. Santana, and Manuel F. Navedo
Circulation Research
Volume 114(4):607-615
February 14, 2014
Copyright © American Heart Association, Inc. All rights reserved.
A-kinase anchoring protein 150 (AKAP150) is necessary for enhanced arterial tone and
diminished iberiotoxin-sensitive constriction in high-fat diet (HFD) arteries.
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
A-kinase anchoring protein 150 (AKAP150) is necessary for reduced large-conductance Ca2+activated K+ (BKCa) channel Ca2+ sensitivity and dwell open time in high-fat diet (HFD) arterial
myocytes.
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
A-kinase anchoring protein 150 (AKAP150) is necessary for impaired large-conductance Ca2+activated K+ (BKCa) β1 subunit function and downregulation of β1 subunit expression in highfat diet (HFD) arterial myocytes.
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
Nifedipine and ablation of A-kinase anchoring protein 150 (AKAP150) prevent decreased largeconductance Ca2+-activated K+ (BKCa) channel function by elevated glucose.
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
Anchoring of calcineurin by A-kinase anchoring protein 150 (AKAP150) is necessary for largeconductance Ca2+-activated K+ (BKCa) channel suppression and impaired vasoconstriction in
response to elevated glucose and high-fat diet (HFD).
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
Activation of nuclear factor of activated T-cells c3 isoform (NFATc3) signaling is necessary for
large-conductance Ca2+-activated K+ (BKCa) channel suppression in response to elevated
glucose and high-fat diet (HFD) and proceeds through A-kinase anchoring protein 150
(AKAP150).
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.
Ablation of A-kinase anchoring protein 150 (AKAP150) or disruption of the interaction between
calcineurin (CaN) and AKAP150 attenuates high-fat diet (HFD)–induced elevation of blood
pressure.
Matthew A. Nystoriak et al. Circ Res. 2014;114:607-615
Copyright © American Heart Association, Inc. All rights reserved.