BIO 311c
Introductory Biology
K. Sathasivan
In Class Activity 9.3 Respiration
Oxidative Phosphorylation and Fermentation
Name ____________________________ Discussion: ____________
A. Multiple Choice Questions
1) Which of the following poison can inhibit the generation of Δ [H+]?
A) A poison that would inhibit ATP synthase.
B) An enzyme that would donate electrons to O2
C) Cyanide like chemical that would block the electron transfer
D) A chemical that would make the inner mitochondrial membrane leaky to ions
E) A chemical that mimics glucose but is not metabolized by hexokinase
2) How does the reduction of pyruvate to lactic acid during fermentation allow glycolysis to
continue in the absence of oxygen?
A) Water is formed during this reaction.
B) This reaction is a kinase reaction.
C) This reaction is coupled to the oxidation of NADH to NAD+.
D) This reaction is coupled to the formation of ATP.
E) This reaction is coupled to the reduction of NAD+ to NADH.
3) When we exercise for a long time without rest, the muscle cells become deprived of oxygen.
Which of the following is not produced in a muscle cell under such conditions?
A) NADH
B) ethanol
C) ATP.
D) pyruvate.
E) lactate.
4) Migratory birds store or carry limited amounts of stored energy or food but fly over long
distances. Which of the following process is most utilized in such situations to make the most
amount of ATP for their flying?
A) glycolysis
B) ethanol fermentation
C) lactate fermentation
D) Krebs cycle
E) oxidative phosphorylation
5)
A)
B)
C)
D)
E)
FADH2 produces less ATP each when compared to NADH because _________________
NADH carries 4 electrons whereas FADH2 carry only 2 electrons.
NADH carries 2 electrons whereas FADH2 carry only 1 electron.
Same membrane protein that accepts electrons from these molecules binds at various sites.
FADH2 allows fewer protons transported across inner membrane and hence makes less ATP.
NADH allows fewer protons transported across inner membrane and hence makes more ATP.
B. Short Answer Questions
1. Propose a hypothesis for chronic fatigue syndrome and how you can overcome this.
2. Why the respiratory poisons such as cyanide and carbon monoxide kill the cells or the
organism? Elaborate as to what happens when the electron transport is blocked and why
other pathways in respiration also stop.
3. While animals will be killed by cyanide, plants are not affected by cyanide. Research and
find out the reasons.
4. Sprinters and marathon runners have different types of muscle tissues that use various
processes to make ATP according to their needs. Speculate as to what type of ATP
synthesis mechanisms operate in such individuals.