From: Fructose-1,6-Bisphosphate Preserves Adenosine Triphosphate but Not Intracellular pH during Hypoxia in Respiring
Neonatal Rat Brain Slices
Anesthes. 1998;88(2):461-472.
Figure Legend:
Figure 4. This schematic diagram begins at the bottom left, where a sup 13 C label is shown in bold type at the C2 position of
glucose-6-phosphate ([2-sup 13 C]G6P). Naturally abundant carbon,12C, has no magnetic moment, and is nuclear magnetic
resonance (NMR)-invisible. The position of13C in the next step of glycolysis is shown immediately to the bottom right. The label
remains in the C2 position after phosphoglucose isomerase converts G6P into [2-sup 13 C]fructose-6-phosphate ([2-sup 13 C]F6P);
however, when [2-sup 13 C]G6P traverses the pentose phosphate pathway, decarboxylation puts the 13C label in the C1 position,
causing
the formation
[1-sup 13 C]F6P.
Distinctly
different
chemical
shifts
characterize the13
resonance peaks for these two
Date of download:
7/31/2017
Copyright
© 2017
American
Society
of Anesthesiologists.
AllC-NMR
rights reserved.
isotopomers, i.e., these two molecules that are identical except for the location of an isotope label. Measured relative to the