Thrombin Activates the Sarcolemmal Na+-H+ Exchanger
by Masahiro Yasutake, Robert S. Haworth, Anna King, and Metin Avkiran
Circulation Research
Volume 79(4):705-715
October 1, 1996
Copyright © American Heart Association, Inc. All rights reserved.
A, Representative recording of the C-SNARF-1 fluorescence emission intensity ratio (I580/I640)
from a single cell during exposure to nigericin-containing calibration solutions of varying pH
(numbers indicate pH).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
Reverse-transcription PCR analysis of thrombin receptor mRNA expression.
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
Representative pHi recordings (top) and pHi-vs-JH curves (bottom) obtained during two
consecutive acid pulses in control cells (A, n=11) and cells in which 5 U/mL thrombin was
present throughout the second pulse (B, n=5).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
JH values at an identical pHi of 6.9 after consecutive acid pulses in control cells (C, n=11) and in
cells that were exposed to thrombin (0.2 U/mL, n=7; 1 U/mL, n=6; and 5 U/mL, n=5) throughout
the second pulse.
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
JH values at an identical pHi of 6.9 after consecutive acid pulses in control cells (C, n=11) and in
cells that were exposed to SFLLRN (1 μmol/L, n=4; 5 μmol/L, n=4; and 25 μmol/L, n=8)
throughout the second pulse.
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
Recording of the indo 1 fluorescence emission intensity ratio (I405/I485) from a single cell during
consecutive exposure to field stimulation (left), 5 U/mL of thrombin (middle), and 10 mmol/L of
caffeine (right).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
JH values at an identical pHi of 6.9 after consecutive acid pulses in control cells (C) and in cells
that were exposed to 5 U/mL of thrombin (Th) or 25 μmol/L of SFLLRN (SFLL) throughout the
second pulse, in the absence of PKC inhibitor (left) or in the presence of either 100 nmol/L of
GF109203X (GF, middle) or 5 μmol/L of chelerythrine (CH, right).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
A, Representative pHi recording (n=3) from a single myocyte during two consecutive acid pulses
in the presence of thrombin (5 U/mL).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.
A, Representative pHi recording (n=3) from a single myocyte during two consecutive acid pulses
in the presence of SFLLRN (25 μmol/L).
Masahiro Yasutake et al. Circ Res. 1996;79:705-715
Copyright © American Heart Association, Inc. All rights reserved.