Annexure ‘CD – 01’
FORMAT FOR COURSE CURRICULUM
Course Title: INSTRUMENTAL ANALYTICAL TECHNIQUES
Course Code: to be decided later
Credit Units:4
Course Objectives: The aim of this course unit is to introduce the student to a wide variety of modern analytical
techniques used in environmental science research. It is designed to couple theory of equipment operation with a basic
understanding of the chemical principles involved.
L
T
P/S
SW/F
W
4
-
-
-
Pre-requisites: Basic knowledge of chemistry, physics
Student Learning Outcomes: By the end of the course students will:




Review the basics of the major analytical techniques and how the techniques can be applied in environmental sciences.
Review sample preparation, Instrument standardization and data analysis for each technique.
Calculate working solution volumes and concentrations, generate linear regressions, and execute computerized spreadsheets.
Interpretation and analysis of output data from instruments.
Course Contents/Syllabus:
Weightage (%)
Module I
Sampling and Data Analysis: Sampling of Air, Water and Soil; Sample Preparation: Interferences and Detection
Limits, Quality Control in the Laboratory: Equipment Calibration and maintenance, Matrix spike and Blank samples;
Sampling equipments; Data treatment such as Accuracy, Precision, Standard deviation, Types of errors, Minimization
of errors, Significant figures, Criteria for rejection of data.
10
Module II
15
Physiochemical parameters and Microscopy – Definition and determination of Conductivity, pH, DO, BOD, COD,
Measuring instruments. Principles and application of Gravimetric, Titrimetry, Microscopy: Principle and application of
light, phase contrast, fluorescence, scanning and transmission electron microscopy, atomic force microscopy, confocal
microscopy, fixation and staining.
TOTAL
CREDIT
UNITS
4
Module III
30
Colorimetry and Spectrophotometry – Theory and instrumentation; Theory, instrumentation and application of
Atomic Absorption Spectrometry, Flame Emission Spectrometry and Inductively Coupled Plasma Mass Emission
Spectrometry; X-ray florescence, X-ray diffraction, IR, NMR and ESR spectroscopy.
Module IV
Separation Techniques: Principle and process of solvent extraction, Extraction reagents and Practical applications;
Chromatography – principle and application of Thin layer and Ion exchange chromatography, Column
chromatography), Gas chromatography, Gas-liquid chromatography, GC-MS, High pressure liquid chromatography.
Liquid Columen Chromatography and mass spectrophotometry (LC-MS).
30
Module V
15
Electrophoresis (Paper and Gel) Techniques, solid and liquid scintillation, Auto radiography, Centrifugation and its
types, Ultracentrifugation, Instruments for Nephelometry and Turbidimetry and their applications, DOC analyzer,
Elemental Analyzer, Most Probable Number (MPN) for water quality monitoring.
Pedagogy for Course Delivery: Course will be delivered through both power point presentation and black board teaching. Students will be provided with
reference materials in form of handouts or PPTs. Regular internal assessment will be done through class seminar and class test for evaluating the continuous
progress of students.
Lab/ Practicals details, if applicable: NA
Assessment/ Examination Scheme:
Theory L/T (%)
Lab/Practical/Studio (%)
Total Marks
100%
100
Theory Assessment (L&T):
Continuous Assessment/Internal Assessment
Components (Drop
down)
Weightage (%)
End Term
Examination
Class Test
Home Assignment
Seminar/viva/Quiz
Attendance
10%
5%
10%
5%
Lab/ Practical/ Studio Assessment: NA
Text & References:
 Standard Methods for Examination of water and waste water : A. E. Greenberg, A. D. Eaton; APHA, AWWA,WEF
 Laboratory Manual for the Examination of Water, Waste water and soil : H. H. Rump and H. Krist; V C H Publication
 Instrumental Methods of Analysis: H.H.Willard, L.Merritt, J.A.Dean, F.A. Settle; CBS Publishers & Distributors Pvt. Ltd.
 Environmental Chemistry; A.K. De , New Age International, New Delhi. 2000.
 Analytical Chemistry; W.J. Murphy, American Chemical Society, USA. 1977.
 Principles of Instrumental Analysis,:D. A. Skoog, F. J. Holler and S. R. Grouch. Brook/Cole Pub Co., 2006.
 Environmental Analytical Chemistry, 2nd Edition, F.W. Fifield, P.J. Blackwell Science, Malden, MA, 2000.
 A Textbook of Environmental Chemistry, V. Subramanian, I K International Publishing House Pvt. Ltd,New Delhi.2011
70%