Supplemental figure 1
A
B
kDa
150
100
75
Wt
Fix-
kDa
150
100
Root
Nodule
- NO3- + NO3- - NO3- + NO3-
C
kDa
75
50
50
37
37
25
SNP (mM)
1
10
150
100
75
50
0
37
25
25
Supplemental Figure1: Western blots of total soluble proteins of M.truncatula root nodules
(10 mg per lane). The position of the molecular weight markers is indicated. A- Total soluble
proteins were extracted from root nodules formed by wild type (WT) and fixJ (Fix-) mutant
strains of S. meliloti. B- Total soluble proteins were extracted from roots and from 21 day old
root nodules collected from plants treated for 2 days with NH4NO3. C-Total soluble proteins
were extracted from root nodules collected from plants treated with increasing concentrations
of SNP.