FluoProbes
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FT-091640
Oxidative phosphorylation uncouplers
Introduction
Oxidative phosphorylation combines two simultaneous processes; the electron transport chain and
chemiosmotic coupling. The electron transport chain (also known as the respiratory chain) comprises 4
complexes located in the inner mitochondrial membrane, which pass electrons from one electron acceptor to the
next to generate a transmenbranar redox potential while the electron loss energy Typically, NADH and FADH 2,
produced from glycolysis and the Krebs cycle, release electrons at certain points in the chain.
Uncoupler
Products
CCCP
FCCP (mClCCP)
Enterostatin
Malonoben
Inhibitor
Products
Rotenone .
Biological activity
Cat.#
Oxidative phosphorylation uncoupler
A very potent uncoupler of oxidative phosphorylation in
mitochondria
Binds to β-subunit of F1-ATPase
Oxidative phosphorylation uncoupler
Biological activity
09164A
333861
Inhibits complex I of the mitochondrial electron transport chain
02859A
MV8890
RL2400
Cat.#
Products descriptions
Technical and scientific information
CCCP #09164
Name :
Catalog Number :
Carbonyl cyanide 3-chlorophenylhydrazone
Structure :
Molecular Weight :
Solubility:
Storage:(Z)
CAS: [555-60-2]
MW= 204.62
Soluble to 100 mM in DMSO
Room Temperature, protect from moisture
091640, 500mg
Biological Activity:
Widely used uncoupler of oxidative phosphorylation
References:
Heytler and Prichard (1962) A new class of uncoupling agents - carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Comm. 7 272.
FCCP (mCl CCP) #33361
Name :
Carbonyl cyanide 4(trifluoromethoxy)phenylhydrazone
Catalog Number :
333861, 10mg
Structure :
Molecular Weight :
Solubility:
CAS:[370-86-5]
MW= 251.1
Soluble to 100 mM in DMSO and to
5 mM in ethanol
Room Temperature, protect from moisture
Storage:(Z)
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FT-091640
Biological Activity:
A very potent uncoupler of oxidative phosphorylation in mitochondria
References:
Heytler and Pritchard (1962) A new class of uncoupling agents-carbonyl cyanide phenylhydrazones. Biochem.Biophys.Res.Comm. 7 272.
Connop et al (1999) Novel effects of FCCP [carbonyl cyanide p-(trifluoromethoxy)phenylhydrazone] on amyloid precursor protein
processing. J.Neurochem.72 1457.
Maloneben #RL240
Name :
3,5-Di-tert-butyl-4hydroxybenzylidenemalononitrile
Catalog Number :
FP-RL2400, 10mg
Structure :
Molecular Weight :
Solubility:
Storage:(Z)
CAS:[10537-47-0]
MW= 282.38
Soluble to 75 mM in DMSO
Room Temperature, protect from moisture
Biological Activity:
Potent uncoupler of oxidative phosphorylation
References:
Muraoka and Terada (1972) 3,5-Di-tert-butyl-4-hydroxybenzylidemalonitrile: a new powerful uncoupler of respiratory-chain
phosphorylation. Biochim.Biophys.Acta 275 271.
Terada et al (1975) Some biochemical and physiochemical properties of the potent uncoupler SF 6847 hydroxybenzylidemalonitrile.
Biochim.Biophys.Acta 387 519.
Terada et al (1981) The interaction of highly active uncouplers with mitochondria. Biochim.Biophys.Acta 639 225.
Enterostatin #MV889
Name :
Ala-Pro-Gly-Pro-Arg
Sequence: APGPR
Catalog Number :
MV8890, 2mg
Structure :
Molecular Weight :
Solubility:
CAS:[117830-79-2]
MW= 496.57
Soluble to 100 mM in DMSO and to
5 mM in ethanol
-20°C, protect from moisture
Storage:(Z)
Biological Activity:
N' terminal peptide fragment of procolipase that binds to the β-subunit of F1-ATPase. Activates the ERK and
cAMP signaling pathways, and downregulates expression of Krüppel-like factor 4 (KLF4) and agouti-related
peptide (AgRP) in vitro. Inhibits insulin secretion from pancreatic β-cells by downregulating expression of
dynamin2 and altering protein trafficking. Reduces dietary fat intake via activation of CCK1, induces satiety,
enhances memory-consolidation and exhibits hypocholesterolemic activity in vivo. Orally active. Technical Data
M.Wt:496.57Formula:Storage:Store at -20°CCAS No:[]
References:
Takenaka et al (2008) Enterostatin reduces serum cholesterol levels by way of a CCK(1) receptor-dependent mechanism. Peptides 29 2175.
Park et al (2009) Enterostatin affects cyclic AMP and ERK signaling pathways to regulate Agouti-related Protein (AgRP) expression.
Peptides 30 181.
Park et al (2009) Enterostatin alters protein trafficking to inhibit insulin secretion in Beta-TC6 cells. Peptides In press.
Rotenone #61964
Name :
Catalog Number :
Rotenone
Structure :
Molecular Weight :
CAS:[83-79-4]
MW= 394.42
02859A, 50mg
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FT-091640
Solubility:
Storage:(Z)
®
Soluble to 100 mM in DMSO and to (2R,6aS,12aS)-1,2,12,12a-Tetrahydro-8,9-dimethoxy-2(1-methylethenyl)-[1]benzopyrano[3,4-b]furo[2,35 mM in ethanol
h][1]benzopyran-6(6aH)-one
Room Temperature, protect from moisture
Biological Activity:
Mitochondrial electron transport chain inhibitor (IC50 = 1.7 - 2.2 μM at complex I). Inhibits NADH oxidation by
cardiac sarcoplasmic reticulum (IC50 = 3.4 nM). Commonly used pesticide and induces Parkinsonism in animal
models. Cell-permeable and brain penetrant.
References:
Cherednichenko et al (2004) NADH oxidase activity of rat cardiac sarcoplasmic reticulum regulates calcium-induced calcium release.
Circ.Res. 94 478.
Uversky (2004) Neurotoxicant-induced animal models of Parkinson's disease: understanding the role of rotenone, maneb and paraquat in
neurodegeneration. Cell Tissue Res. 318 225.
Gomez et al (2007) Pesticides and impairment of mitochondrial function in relation with the parkinsonian syndrome. Front.Biosci. 12 1079.
Directions for use
Guidelines for use
Protocol may be found in the literature.
Related / associated products and documents
See Products Highlights, BioSciences Innovations catalogue and e-search tool.
Ordering information
Catalog size quantities and prices may be found at www.interchim.com/
Please inquire for higher quantities (availability, shipment conditions).
For any information, please ask : Interchim; Hotline : +33(0)4 70 03 73 06
Disclaimer : Materials are sold for research use only, and are not intended for food, drug, household, or cosmetic use. Interchim is not
liable for any damage resulting from handling or contact with this product.
Rev.P08E-K12E
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