Curriculum Vitae Personal information Surname / First name Address Prota Neli Thorbeckestraat 218, 6702BZ Wageningen, The Netherlands Telephone +31-317843097 Mobile: +31-622075394 Telephone Work +31-317481084 Fax: +31-317418094 E-mail Nationality Date of birth Gender [email protected], [email protected] Italian, Croatian 29 April 1985 Female Professional experience Dates Occupation or position held Project title September 2007 – present PhD fellowship Molecular breeding of insect resistance in vegetable crops Topics: genetic engineering of secondary metabolism pathways; GC/MS analysis of secondary metabolites; enzymatic assay with terpene synthases; developing a strategy for hunting for an unknown gene of the Cyt P450 family; Name and address of employer/supervisor Type of business or sector Dates Occupation or position held Main activities and responsibilities Prof. Dr. H. J. Bouwmeester, Laboratory of plant physiology/Plant Research International, Wageningen University and Research centre, Droevendalsesteeg 1 , 6708BP Wageningen, the Netherlands – Tel. +31317486001 Metabolic regulation, Plant Physiology September 2006 – June 2007 Internship for the Master degree FRET experiments on a Type-1 copper-protein from Streptomyces coelicolor Techniques: modelling of a protein with unknown 3D structure, primers design, PCR, Mutagenesis with Overlap Extension PCR, Agarose gel electrophoresis, Transformation of Escherichia coli, Protein purification, SDS-PAGE, FRET analysis with fluorescence spectrophotometer, Apo-protein preparation and Zn-reconstitution experiments, in vivo labelling of the protein Name and address of employer/supervisor Type of business or sector Dates Occupation or position held Main activities and responsibilities Prof. G. W. Canters, MetProt Group, LIC, Einsteinweg 55, 2333 Leiden, the Netherlands – Tel. +31715274256 Metallo-protein research, Institute of Chemistry April 2005 – July 2005 Internship for the Bachelor degree Expression pattern determination of Protochlorophyllide oxidoreductase in Zea mais during the greening process by monitoring the mRNA level Techniques: DNA and RNA purification from plant tissue, PCR, RT-PCR, Agarose gel electrophoresis Name and address of employer/supervisor Type of business or sector Dates Occupation or position held Prof. N. Rascio and Dr. N. La Rocca, Dipartimento di Biologia, via Colombo 58, 35100 Padova, Italy – Tel. +390498276273 Plant physiology research, Biology department August 2004 – September 2004 Stage -1- Main activities and responsibilities Name and address of employer/supervisor Type of business or sector Molecular biology techniques: PCR, Agarose gel electrophoresis, Automated processes for determining different strands of HBV, HCV and HGV M. Crovato, Dipartimento Medicina di laboratorio, Azienda ospedaliera S. Maria degli Angeli, via Montereale 24, 33710 Pordenone, Italy – Tel. +390434399281 Clinic immunology and virology, Laboratory medicine department Education and training Dates Title of qualification awarded Principal subjects/occupational skills covered October 2005 – July 2007 Master of science in Molecular Biology Thesis title: Towards in vivo redox sensing - Analysis of the redox state of a cupredoxin with FRET using biarsenical derivates of fluorescein and resorufin - Ten months internship in Leiden, the Netherlands - 15 ECT course in Molecular and cell biology of Plants - 26/30 - 11 ECT course in Biophysics - 30/30 - 9 ECT course in Cell biology - 26/30 - 4 ECT course in Biopolimers structure determination (NMR and Crystallography) – 24/30 Name and type of organisation providing education and training Università degli studi di Padova, Italy Level in national or international classification 102/110 Dates Title of qualification awarded Principal subjects/occupational skills covered October 2002 – July 2005 Bachelor of science in Molecular Biology Thesis title: Molecular characterization of the enzyme Protochlorophyllide NADPH oxidoreductase in maize (Zea mais) - Total of 14 ECT in Bioinformatics - Total of 12 ECT in Molecular biology including a practical course - Total of 10 ECT in Cell biology - Total of 6 ECT in Plant development and physiology - 6 ECT course in Genetic engineering - 3 ECT course in Management and organization of Human resources - 5 ECT course in Risks from Physical Chemical and Biological agents - 4 ECT course in Immunology Name and type of organisation providing education and training Università degli studi di Padova, Italy Level in national or international classification 101/110 Dates Title of qualification awarded Principal subjects/occupational skills covered September 1998 – June 2002 High school degree – Scientific gymnasium Thesis title: Genetic engineering Strong education in Mathematics, Physics and Informatics Name and type of organisation providing education and training Scuola Media Superiore Italiana – Srednja Talijanska Škola, Rijeka, Croatia Level in national or international classification 5/5 Conferences and workshops (more recent at the bottom) Dates Title and place Dates Title and place Presentations 9/10/2006 ANNUAL DUTCH MEETING ON MOLECULAR AND CELLULAR BIOPHYSICS, Lunteren, the Netherlands 11-12/12/2006 50th ANNUAL MEETING PROTEIN RESEARCH STUDY-GROUP, Lunteren, the Netherlands Poster presentation: Lipocyanin, an unusual membrane-bound blue copper protein -2- Dates Title and place Presentations Dates Title and place Dates Title and place Dates Title and place Dates Title and place Dates Title and place 2/03/2007 ALUMNI WORKSHOP, Leiden, the Netherlands Oral presentation: Towards in vivo redox sensing, FRET on Lipocyanin from Streptomyces coelicolor 26/10/2007 PLANT-INSENCT INTERACTION WORKSHOP, Amsterdam SILS, the Netherlands 6/11/2007 EPS Theme 3 symposium, Wageningen, the Netherlands 06/03/2008 Life Science Symposium - Imaging, Leiden, the Netherlands 7-8/04/2008 NOW-ALW Meeting of Experimental Plant Sciences, Lunteren, the Netherlands 24-25/04/2008 GA Symposium – Plants system biology and medicinal plants, Leiden, the Netherlands Courses (more recent on top) Dates Title and place May 2008 – 4 sessions of one day each CAREER ORIENTATION by B. Hertz, Wageningen, the Netherlands Other activity (more recent on top) Dates Title and place February 2008 – Present Teaching Italian course for beginners, ISOW, Wageningen Personal skills and competences Mother tongue(s) Italian, Croatian Other language(s) Self-assessment Understanding European level (*) Listening English C1 Spanish Dutch French Slovenian Proficient user Reading C1 Proficient user B1 Independent User B1 Independent User B1 Independent User A2 Basic User A1 Basic User B1 Independent User B1 Independent User A2 Basic User A1 Basic User A2 Basic User A1 A1 Basic User A2 Basic User A1 Basic User B1 Independent User B1 Independent User A1 Basic User A1 Basic User A1 Basic User A2 Proficient user Basic User C1 Proficient user (*) Common European Framework of Reference for Languages Social skills and competences Writing Spoken production Proficient user Basic User C1 Speaking Spoken interaction Ability to: - Compromise - Influence others - Negotiate - Get people’s attention - Encourage others - Show interest in other people’s ideas and opinions (empathy) -3- C1 Communication skills and competences Personal effectiveness skills and competences Ability to: - Speak in front of broad audiences Sustain a conversation Lead a discussion Express myself fluently in scientific debates Write a scientific report Present my work or that of others in a linear and efficient way Teach Proactiveness; flexibility; ambition; passion in achieving a goal; enthusiastic about progress; interest in a wide variety of subjects; will to travel in Europe and USA; efficient planning; intuition Cognitive skills and competences Good at: - Learning things fast - Learning languages - Structure an oral presentation - Put forward appropriate arguments In general, good memory and skilled in logics, mathematics and grammar Technical skills and competences Knowledge of basic GC/MS analysis and organic extraction of compounds from various plant tissues; Expertise with most of the biochemical and molecular biology techniques, achieved during the various internships; Fluorescence spectroscopy, achieved during the Master internship; Plant propagation by tissue culture and Analysis of plant pigments using paper chromatography, achieved during the course in Molecular and cell biology of Plants Computer skills and competences MS Windows 98, 2000, XP; MS Office: Word, Excel and PowerPoint; Internet explorer and Mozilla firefox; Biological Data Bases; Basics of Origin; basics of Corel PhotoPaint and Adobe Photoshop; basics of programming with Turbo Pascal and Perl; basics of Linux OS. Artistic skills and competences Other skills and competences Driving licence Salsa dance, Piano Hobbies: gardening, cooking, travelling, camping, visiting museums, skiing, swimming, none Additional information Reference: see addresses in the above mentioned internships paragraphs -4-
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