DOC/LP/01/28.02.02
LP-BT 2255
LESSON PLAN
LP Rev. No: 02
Sub Code & Name:
Unit: I
BT2255
Date:
Microbiology
Branch: Biotechnology
Semester : IV
30/12/2013
Page 1 of 6
UNIT-1: Classical genetics
6
Basic of microbial existence; history of microbiology, classification and
nomenclature of microorganism, microscopic examination of microorganisms, light and
electron microscopy; principles of different staining techniques like gram staining, acid
fast, capsular staining, flagellar staining.
OBJECTIVE: To introduce the Basics of Microbiology
Session
No
1.
Introduction to microbiology
Time
(min.)
50
2.
Basic of microbial existence
50
T1(1-4)
SMC, BB & Chalk
3.
History of microbiology
50
T1(12,13)
SMC, BB & Chalk
50
T1(18,19)
SMC, BB & Chalk
4.
Topics to be covered
Classification and nomenclature of
microorganism
Microscopic examination of
5.
microorganisms, light and electron
50
microscopy
Ref
Teaching Method
T1(1,2)
SMC, BB & Chalk
T1(71-74,
76-78)
SMC, BB & Chalk
Principles of different staining
6.
techniques like gram staining, acid fast,
capsular staining, flagellar staining
1
50
T1(98,99)
SMC, BB & Chalk
DOC/LP/01/28.02.02
LP-BT 2255
LESSON PLAN
LP Rev. No: 02
Sub Code & Name:
Unit: II
BT2255
Date:
Microbiology
Branch: Biotechnology
30/12/2013
Semester : IV
Page 2 of 6
UNIT II: MICROBES-STRUCTURE AND MULTIPLICATION
12
Structural organization and multiplication of bacteria, viruses, algae and fungi
with a special mention of life history of Actinomycetes, yeast, Mycoplasma and
Bacteriophage.
OBJECTIVE: To discuss in detail the structures and multiplication of microbes
Session
No
Topics to be covered
Structural organization of
7.
bacteria
Time
(min)
Ref
Teaching Method
50
T3(44-50)
SMC, BB & Chalk
8.
Multiplication of bacteria
50
T3(44-50)
SMC, BB & Chalk
9.
Structural organization of virus
50
T3(358,59)
SMC, BB & Chalk
10.
Replication of viruses
50
T3(4,7)
SMC, BB & Chalk
11.
Structural organization of algae
50
T3(556,57)
SMC, BB & Chalk
12.
Multiplication of algae
50
T3(558)
SMC, BB & Chalk
13.
Structural organization of fungi
50
T3(539,40)
SMC, BB & Chalk
14.
Replication of fungi
50
T3(546-49)
SMC, BB & Chalk
15.
Actinomycetes
50
T3(589-602)
SMC, BB & Chalk
16.
Yeast
50
T3(539)
SMC, BB & Chalk
17.
Mycoplasma
50
T3(504,505)
SMC, BB & Chalk
18.
Bacteriophage
50
T3(371-73)
SMC, BB & Chalk
2
DOC/LP/01/28.02.02
LP-BT 2255
LESSON PLAN
LP Rev. No: 02
Sub Code & Name:
Unit: III
Date:
BT2255 Microbiology
Branch: Biotechnology
Semester : IV
30/12/2013
Page 3 of 6
UNIT III MICROBIAL NUTRITION, GROWTH AND METABOLISM
12
Nutritional requirements of bacteria and different media used for bacterial
culture; growth curve and different methods to quantitate bacterial growth, aerobic and
anaerobic bioenergetics and utilization of energy for biosynthesis of important molecules.
OBJECTIVE: To impart knowledge of microbial growth and their metabolism
Session
No
19.
20.
21.
22.
23.
24.
Topics to be covered
Nutritional requirements of bacteria I
Nutritional requirements of bacteria
II
Different types of media used for
bacterial culture I
Different types of media used for
bacterial culture II
Bacterial growth curve
Different methods to quantitate
bacterial growth
Time
(min)
50
T1(`188-190)
OHP, BB & Chalk
50
T1(`188-190)
OHP, BB & Chalk
50
T1(`188-190)
OHP, BB & Chalk
50
T1(`188-190)
OHP, BB & Chalk
50
T1(207-12)
OHP, BB & Chalk
50
T1(207-12)
OHP, BB & Chalk
Ref
Teaching Method
25.
Aerobic bioenergetics
50
T1(231-33)
OHP, BB & Chalk
26.
Anaerobic bioenergetics
50
T1(231-33)
OHP, BB & Chalk
50
T1(217-19)
OHP, BB & Chalk
50
T1(217-19)
OHP, BB & Chalk
27.
28.
Utilization of energy for biosynthesis
of smaller molecules
Utilization of energy for biosynthesis
of smaller molecules I
3
29.
30.
Utilization of energy for biosynthesis
of macro molecules II
Utilization of energy for biosynthesis
of organic cell materials
4
50
T1(217-19)
OHP, BB & Chalk
50
T3(225-245)
OHP, BB & Chalk
DOC/LP/01/28.02.02
LP-BT 2255
LESSON PLAN
LP Rev. No: 02
Sub Code & Name:
Unit: IV
Date:
BT2255 Microbiology
Branch: Biotechnology
Semester : IV
30/12/2013
Page 4 of 6
UNIT IV: CONTROL OF MICRO ORGANISMS
6
Physical and chemical control of microorganisms; host - microbe interactions;
anti bacterial anti fungal and anti viral agents, mode of action and resistance to
antibiotics. Clinically important micro organisms.
OBJECTIVE: To impart the knowledge of host – microbe interactions
Session
No
31.
32.
33.
34.
35.
36.
Topics to be covered
Physical control of
microorganisms
Chemical control of
microorganisms
Host - microbe interactions
Anti bacterial anti fungal and
anti viral agents
Mode of action and resistance to
antibiotics
Clinically important micro
organisms
Time
(min)
Ref
50
T1(317)
OHP, BB & Chalk
50
T1(317)
OHP, BB & Chalk
50
T3(717-723)
T3(786-92,
795-96)
T3(786-92,
795-96)
OHP, BB & Chalk
T3(885-910)
OHP, BB & Chalk
50
50
50
5
Teaching Method
OHP, BB & Chalk
OHP, BB & Chalk
DOC/LP/01/28.02.02
LP-BT 2255
LESSON PLAN
LP Rev. No: 02
Sub Code & Name:
Unit: V
BT2255
Date:
Microbiology
Branch: Biotechnology
Semester : IV
30/12/2013
Page 5 of 6
UNIT V INDUSTRIAL AND ENVIRONMENTAL MICROBIOLOGY
9
Primary metabolites; secondary metabolites and their applications Preservation of
food; production of penicillin, alcohol, vit B12; biogas; bioremediation; leaching of ores
by microorganisms; bio fertilizers and bio pesticides; microorganisms and pollution
control.
OBJECTIVE: To impart the knowledge of industrial and environmental biotechnology
Session
No
37.
38.
39.
40.
41.
42.
43.
44.
45.
Topics to be covered
Time
(min)
Ref
50
T3(1068-1075)
SMC, BB & Chalk
50
T3(1068-1075)
SMC, BB & Chalk
50
50
50
50
T3(1028-1032)
T3(1070-1071)
T3(1041-1045)
T3(1075-1077)
SMC, BB & Chalk
SMC, BB & Chalk
SMC, BB & Chalk
SMC, BB & Chalk
50
T3(1081)
SMC, BB & Chalk
50
50
T3(1083)
SMC, BB & Chalk
SMC, BB & Chalk
Primary metabolites and their
applications
Secondary metabolites and
their applications
Preservation of food
Production of penicillin
production of alcohol, vit B12
Biogas; bioremediation
Microorganisms and pollution
control
Biosensor
Review
6
Teaching Method
DOC/LP/01/28.02.02
Course delivery plan:
WEEK
1
UNITS
2
I
3
4
5
6
7
II
8
9
III
10
11
12
13
IV
Text Books:
1. Pelczar MJ, Chan ECS and Krein NR, Microbiology, Tata McGraw-Hill
Edition,New Delhi, India
2. Prescott LM, Harley JP, Lein DA, Microbiology 3rd Edition, WmC.Brown
Publishers, 1996
3. Talaron K, Talaron A, Casita, Pelczar and Reid. Foundations in Microbiology,
W.C. Brown Publishers, 1993
Reference:
1. Mackie and Mccartney, practical medical microbiology, 14th Edition,
Internationalstudent edition, Churchill living stone 1996
Prepared by
Approved by
Mr.N.Sathish
Asst.Professor
Prof. Sulochana somasundaam
HOD of Biotechnology
30/12/2013
30/12/2013
Signature
Name
Designation
Date
7
14
V
15