Supplemental Table 11. Gene designations, functions and protein identifiers for key enzyme systems known to be or potentially
involved in methylotrophy
Gene name
Protein/
function
JLW8
Methanol oxidation
mxaF
MDH large subunit
mxaJ
essential for MDH function
mxaG
cytochrome, electron acceptor from MDH
mxaI
MDH small subunit
mxaR
chaperonin
mxaS
unknown
mxaA
essential for Ca2+ insertion into MDH
mxaC
essential for Ca2+ insertion into MDH
mxaK
essential for Ca2+ insertion into MDH
mxaL
essential for Ca2+ insertion into MDH
mxaD
xoxF
unknown
MxaF homolog
Protein identifiers
301
Mmol_1107
Mmol_1541
Mmol_1106
Mmol_1542
Mmol_1105
Mmol_1543
Mmol_1104
Mmol_1544
Mmol_1103
Mmol_1545
Mmol_1102
Mmol_1546
Mmol_1770
Mmol_2048
-
M301_1093
M301_2233
M301_1092
M301_2232
M301_1091
M301_2231
M301_1090
M301_2230
M301_1089
M301_2229
M301_1088
M301_2228
M301_1208
M301_2463
M301_1659
SIP3-4
Msip34_0734
Msip34_0735
Msip34_0736
Msip34_0737
Msip34_0738
Msip34_0870
Msip34_2692
Msip34_0739
Msip34_0869
Msip34_2693
Msip34_0740
Msip34_0868
Msip34_2694
Msip34_0741
Msip34_0867
Msip34_2695
Msip34_0742
Msip34_0866
Msip34_2696
Msip34_0743
Msip34_0865
Msip34_2697
Msip34_0744
Msip34_0016
Msip34_2348
Msip34_2549
Msip34_1813
xoxJ
xoxG
MxaJ homolog
MxaG homolog
Pyrroloquinoline quinone (PQQ) biosynthesis
pqqA
PQQ synthesis
pqqB
pqqC
pqqD
pqqE
pqqF
pqqG
PQQ synthesis
PQQ synthesis
PQQ synthesis
PQQ synthesis
PQQ synthesis
PQQ synthesis
Mmol_2047
Mmol_2045
Mmol_2046
-
M301_2462
M301_2459
M301_2460
M301_2461
-
Msip34_2347
Msip34_2346
Msip34_0015
Msip34_2345
Mmol_0021
Mmol_0459
Mmol_0794
Mmol_0993
Mmol_0994
Mmol_0995
Mmol_0996
Mmol_0997
Mmol_1750
Mmol_1751
M301_0054
M301_1191
M301_2123
M301_2651
M301_1192
M301_1193
M301_1194
M301_1195
M301_2041
M301_2042
Msip34_0020
Msip34_1529
Msip34_2759
Msip34_2777
Msip34_1528
Msip34_1527
Msip34_1596
Msip34_1525
Msip34_1921
Msip34_1922
Methylamine oxidation
(methylamine dehydrogenase, MADH)
mauF
unknown
Mmol_1576
mauB
MADH large subunit
Mmol_1575
mauE
essential for small subunit maturation
Mmol_1574
mauD
essential for small subunit maturation
Mmol_1573
mauA
MADH small subunit
Mmol_1572
mauG
TTQ biosynthesis
Mmol_1571
mauL
unknown
Mmol_1570
mauM
unknown
Mmol_1569
mauN
unknown
Mmol_1568
mauO
cytochrome
Mmol_1567
Methylamine oxidation
(N-methylglutamate pathway)
mgdA
N-methylglutamate DH sub. A
-
-
M301_1421
-
Msip34_2428
mgdB
mgdC
mgdD
gma
mgsA
mgsB
mgsC
N-methylglutamate DH sub. B
N-methylglutamate DH sub. C
N-methylglutamate DH sub. D
g-glutamylmethylamide synthase
N-methylglutamate synthase sub. A
N-methylglutamate synthase sub. B
N-methylglutamate synthase sub. C
H4MPT-linked formaldehyde oxidation
fhcB
Fhc, subunit B
fhcA
Fhc, subunit A
orf
FMN-binding protein
fhcD
Fhc, subunit D
fhcC
Fhc, subunit C
mptG
-RFAP synthase
mtdB
methylene H4MPT dehydrogenase
orfY
unknown
mch
methenyl H4MPT cyclohydrolase
orf5
biosynthesis of H4MPT
orf7
unknown
fae
formaldehyde activating enzyme
orf17
orf1
orf9
pabB
orf21
pcbD
orf22
orf19
orf20
afp
unknown
unknown
biosynthesis of H4MPT
para-aminobenzoate synthase component I
biosynthesis of H4MPT
unknown
biosynthesis of H4MPT
biosynthesis of H4MPT
biosynthesis of H4MPT
dihydromethanopterin reductase
-
M301_1420
M301_1419
M301_1418
M301_1417
M301_1416
M301_1415
M301_1414
Msip34_2427
Msip34_2426
Msip34_2425
Msip34_2424
Msip34_2423
Msip34_2422
Msip34_2421
Mmol_0862
Mmol_0861
Mmol_0860
Mmol_0859
Mmol_0858
Mmol_1347
Mmol_1346
Mmol_1345
Mmol_1344
Mmol_1343
Mmol_1342
Mmol_1253
Mmol_2056
Mmol_1336
Mmol_1335
Mmol_1334
Mmol_1333
Mmol_1332
Mmol_1331
Mmol_0899
Mmol_0898
Mmol_0897
Mmol_0896
M301_0913
M301_0912
M301_0911
M301_0910
M301_0909
M301_1557
M301_1556
M301_1555
M301_1554
M301_1553
M301_1552
M301_0896
M301_1343
M301_1545
M301_1544
M301_1543
M301_1542
M301_1541
M301_1540
M301_0953
M301_0952
M301_0951
M301_0950
Msip34_1506
Msip34_1507
Msip34_1508
Msip34_1509
Msip34_1505
Msip34_1504
Msip34_1503
Msip34_1502
Msip34_1501
Msip34_1500
Msip34_1495
Msip34_1496
Msip34_1494
Msip34_1493
Msip34_1492
Msip34_1491
Msip34_1490
Msip34_1489
Msip34_1682
Msip34_1683
Msip34_1684
Msip34_1685
fae2
fae3
formaldehyde activating enzyme homolog
formaldehyde activating enzyme homolog
Formate oxidation
fdh1A
FDH1, dehydrogenase alpha subunit
fdh1B
FDH1, beta subunit
fdh1C
FDH1, gamma subunit
fdh1D
FDH1, accessory protein
fdh1E
FDH1, delta subunit
fdh4A
FDH4, dehydrogenase alpha subunit
fdh4B
FDH4-associated protein
Mmol_0024
-
M301_2758
M301_1423
Msip34_2608
Msip34_2416
Mmol_2033
Mmol_2034
Mmol_2035
Mmol_2032
Mmol_2031
Mmol_0470
Mmol_0469
M301_2447
M301_2448
M301_2449
M301_2446
M301_2445
-
Msip34_1179
Msip34_1178
Msip34_1177
Msip34_1180
Msip34_1181
Msip34_1600
Msip34_1599
Ribulose monophosphate cycle for formaldehyde assimilation/oxidation
hps1
hexulosephosphate synthase
Mmol_0313
hps2
Mmol_1338
hpi
hexulosephosphate isomerase
Mmol_1337
tal
transaldolase
Mmol_1339
pgi
glucose 6-phosphate isomerase
Mmol_0827
zwf
glucose 6-phosphate dehydrogenase
Mmol_1527
pgl
6-phosphogluconolactonase
Mmol_1526
gndA
6-phosphogluconate dehydrogenase (NAD) gndB
6-phosphogluconate dehydrogenase (NADP)
Mmol_0143
edd
6-phosphogluconate dehydratase
Mmol_1727
eda
2-keto 3-deoxy 6-phosphogluconate aldolase
Mmol_1726
tkt
transketolase
Mmol_1980
rpe
ribulosephosphate 3-epimerase
Mmol_2239
ppi
ribose 5-phosphate isomerase
Mmol_0287
Mmol_1429
M301_0304 Msip34_0268
M301_1547 Msip34_1498
M301_1546 Msip34_1497
M301_1548 Msip34_1499
M301_1501 Msip34_1269
M301_1112 Msip34_1093
M301_1114 Msip34_1095
Msip34_1094
M301_2666 M301_2019 Msip34_1897
M301_2018 Msip34_1896
M301_2400 Msip34_0483
M301_2582 Msip34_2516
M301_0182 Msip34_0164
M301_1566 Msip34_1138
Methylcitric acid cycle
prpD
Methylcitrate dehydratase
prpC
Methylcitrate synthase
prpB
Methylisocitrate lyase
cyl1
Citrate lyase-like
M301_0686
M301_0687
M301_0688
M301_0689
Mmol_0748
Mmol_0749
Mmol_0750
Mmol_0751
-
maoC
cyl2
gntR
mdh
sdhC
sdhD
sdhA
sdhB
orf
cyl3
prpE
fum
orf
acn
Dehydratase
Citrate lyase-like
Transcriptional regulator
Malate dehydrogenase
Succinate dehydrogenase, C subunit
Succinate dehydrogenase, D subunit
Succinate dehydrogenase, A subunit
Succinate dehydrogenase, B subunit
Hypothetical protein
Citrate lyase-like
Propionyl-CoA synthase
Fumarase
Hypothetical protein
Aconitate hydratase
MDH, methanol dehydrogenase; H4
Mmol_0752
Mmol_0753
Mmol_0754
Mmol_0756
Mmol_0757
Mmol_0758
Mmol_0759
Mmol_0760
Mmol_0761
Mmol_0762
Mmol_0763
Mmol_0764
Mmol_0765
Mmol_0766
-
M301_0690
M301_0691
M301_0692
M301_0693
M301_0694
M301_0695
M301_0696
M301_0697
M301_0698
M301_0699
M301_0700
M301_0701
M301_0702
M301_0703
Msip34_2203
-
-ribofuranosylaminobenzene.