Data for Six Dendritic Spines
Size
Maximal Excursion
Average
Between Frames
Overall
Spine
Length Width Length Width Length Width
1
1.0 μm 1.8 μm 6%
7%
26%
22%
2
1.7 μm 1.5 μm 8%
8%
19%
29%
3
1.2 μm 1.7 μm 8%
8%
18%
10%
4
1.1 μm 1.2 μm 18%
11%
39%
21%
5
1.3 μm 1.0 μm 9%
11%
28%
35%
6
1.1 μm 1.0 μm 10%
14%
20%
31%
• Develop anti-bodies
against candidate protein
• Add in gold particleantibody complex
• Use SEM to locate protein