From: Production of Fibronectin and Tenascin Isoforms and Their Role in the Adhesion of Human Immortalized Corneal
Epithelial Cells
Invest. Ophthalmol. Vis. Sci.. 2003;44(8):3317-3325. doi:10.1167/iovs.02-1146
Figure Legend:
Western blot analysis of ECM produced by HCE cells. Western blot analysis of the ECM, produced by deoxycholate
treatment, showed a distinct band of Mr 240,000 with mAb to EDA-Fn (lane 1) and a similar weak band with mAb to OncFn (lane 2). Control Western blot analysis of ECM produced by human embryonic fibroblasts showed a similar M r
240,000 band with mAb to Onc-Fn (lane 3). mAb to both subunits of Tn-C showed a distinct reaction with Tn-CH (lane 4)
and a very weak reaction with Tn-CL (lane 4), whereas mAb against the large subunit of Tn-C showed only the high
molecular weight band (lane 5). Control immunoblots with the two mAbs with Tn-C purified from the culture supernatant
of U251Mg
glioma
cells showed, respectively,
both subunits
of Tn-C
(laneand
6) Ophthalmology
and the high molecular
bandreserved.
Date
of download:
7/31/2017
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in Vision
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3
(lane 7). Left: molecular weight standards of the control protein (10 ).