THE ASSESSMENT OF LIPID PEROXIDATION
IN TUMOR-BEARING RATS AFTER PLASMONIC
PHOTOTHERMAL TREATMENT
WORKING GROUP
Alla B. Bucharskaya, Saratov State Medical University , Russia
Galina A. Afanasyeva, Saratov State Medical University, Russia
Alexey V. Ivlichev, Saratov State Medical University, Russia
Dmitry A. Mudrak, Saratov State Medical University, Russia
Nikita A. Navolokin, Saratov State Medical University, Russia
Georgy S. Terentyuk, Saratov State Medical University, Saratov State
University, Russia
Galina N. Maslyakova, Saratov State Medical University, Russia
Boris N. Khlebtsov, Institute of Biochemistry and Physiology of Plants
and Microorganisms RAS, Russia
Nikolai G. Khlebtsov, Institute of Biochemistry and Physiology of Plants
and Microorganisms RAS, Saratov State University, Russia
Alexey N. Bashkatov, National Research Saratov State University, Russia
Elina A. Genina, National Research Saratov State University, Russia
Valery V. Tuchin, National Research Saratov State University, Russia
THE AIM OF STUDY
TEM images of gold nanorods
The aim of study was to assess
the the impact of plasmonic
photothermal treatment (PPTT)
after a single and multiple
intravenous injection of
polyethylene glycol (PEG) coated gold nanorods (GNRs)
on indicators of peroxidation in
the serum of rats with
transplanted liver cancer PC-1.
MATERIALS AND METHODS
The experiments were performed at Centre of Collective
Use of Saratov State Medical University on outbred albino
male rats weighing 180-220 g. GNRs: 41±8 nm (length)
and 10±2 nm (diameter), concentration of 400 μg/ml were synthesized in a laboratory of nanobiotechnologies
(IBPPM RAS). 24 h after last i.v. injection of GNRssuspension, the tumors were irradiated by the NIR 808-nm
diode laser LS-2-N-808-10000 (Laser Systems, Ltd.,
Russia) during 15 min at power density 2.3 W/cm2. The
lipid peroxidation indicators: malondialdehyde (MDA), lipid
hydroperoxide (LHP), the average molecular mass (MSM)
were evaluated by standart spectrophotometric methods in
blood serum of rats.
DESIGN OF EXPERIMENT
The model of liver cancer (cholangiocarcinoma line PC-1)
was reproduced by transplantation of 25% cell
suspension, obtained from the bank of tumor strains of
Russian Cancer Research Center n.a. N.N. Blokhin. When
the tumor reached a diameter of 3.0 ± 0.3 cm3 the rats
were divided into 4 groups (6 animals in group) : group 1
– without treatment, group 2 - with a single injection of 1
mL of GNR-suspension, group 3 - with double injections of
1 mL of GNR-suspension, group 4 - with triple injections of
1 mL of GNR-suspension. The half of animals from 2,3,4,
groups 24 h after last i.v. injection was withdrawn from
the experiment, remaining animals were treated by PPTT
and were withdrawn from the experiment 24 after PPTT.
PC-1 +triple
i.v.GNRs+PPTT
PC-1 +double
i.v.GNRs+PPTT
PC-1 +single
i.v.GNRs+PPTT
PC-1 +triple
i.v.GNRs
PC-1 +double
i.v.GNRs
PC-1 +single
i.v.GNRs
PC-1
control
RESULTS
10
9
8
7
6
5
4
3
2
1
0
MDA
LHP
AWM
The indicators of lipid peroxidation: MDA(pmol/l), GPL
(U/ml), AWM (U/ml) in blood serum
RESULTS
The significant increase in the level of lipid
hydroperoxides (LHP) was found in blood serum
of rats with transplanted liver cancer PC-1 after
single and repeated intravenous injection of
GNRs. Further increase of lipid hydroperoxides
(LHP) was revealed 24 h after PPTT with single
and repeated intravenous injection of GNRs. It
noted along with increased levels of MDA and
indicates the activation of lipid peroxidation as a
result of PPTT exposure on tumor cells.
CONCLUSION
The significant differences in levels of
malondialdehyde (MDA) and lipid hydroperoxides
(LHP) were revealed in blood serum of rats with
transplanted liver cancer after single and repeated
intravenous injection of GNRs and PPT therapy. The
activation of lipid peroxidation confirms the
efficiency of PPTT treatment of transplanted tumors
in animals and allows to recommend the use of
PEGylated gold nanorods as nanosensitizers in
plasmonic photothermal treatment of tumors.
ACKNOWLEDGEMENTS
This study was supported by state task of Russian
Ministry of Health and partially supported by grants
No. 14-13-01167 and 14-15-00186 of the Russian
Scientific Foundation .